ObjectiveTo observe the changes in plasma interleukin-33 (IL-33) in patients with sepsis and its regularity, the association between IL-33 and the infection, and the significance of IL-33 in predicting the prognosis of sepsis.Methods A prospective single-center single-blind clinical study was conducted. Forty patients with sepsis in intensive care unit (ICU) of Shengjing Hospital of China Medical University from May 2012 to January 2013 were enrolled. The patients were divided into general sepsis, severe sepsis and septic shock groups according to the severity of systemic infection and presence of organ dysfunction. The sepsis patients were again divided into 28-day death group and survival group. Ten healthy volunteers and 11 patients with systemic inflammatory response syndrome (SIRS) were enrolled as healthy control and SIRS groups. The levels of procalcitonin (PCT), IL-33, IL-6, IL-1β, tumor necrosis factor-α (TNF-α), and IL-33 receptor sST2 were determined with enzyme linked immunosorbent assay (ELISA) within 3 hours, and 24 hours and 5 days after enrollment to ICU. The acute physiology and chronic health evaluationⅡ (APACHEⅡ) score was calculated. The clinical outcome, length of stay in ICU, and duration of mechanical ventilation were recorded. The relationship between IL-33 and each parameter was analyzed by Spearman analysis. Receiver operating characteristic (ROC) curve was drawn to evaluate IL-33 in predicting the outcome of sepsis.Results Plasma IL-33 in sepsis patients within 3 hours after admission was significantly increased compared with that of the healthy controls and SIRS group (ng/L: 15.43±7.22 vs. 0.67±0.24, 1.25±1.09, bothP< 0.01). Compared with SIRS group, PCT in sepsis group was significantly increased [μg/L: 52.23 (19.69, 73.37) vs. 1.22 (0.69, 3.73),Z = -2.447,P< 0.001]. With exacerbation of illness, APACHEⅡ score, the values of PCT and IL-33 were gradually increased in general sepsis, severe sepsis and septic shock groups, while the length of stay in ICU and the duration of mechanical ventilation were gradually prolonged (P< 0.05 orP< 0.01). The concentration of IL-33 (ng/L) of sepsis patients admitted to ICU within 3 hours, and 24 hours and 5 days of the illness was 15.43±7.22, 11.82±6.16, 5.55±2.25, respectively (F = 4.823,P = 0.004). There was a positive correction between IL-33 within 3 hours after ICU admission and APACHEⅡ score (r = 0.351,P = 0.031), PCT (r = 0.412,P = 0.005), IL-6 (r = 0.535,P = 0.030), IL-1β (r = 0.674,P = 0.030), TNF-α (r = 0.250,P= 0.030), sST2 (r = 0.620,P< 0.001), and length of stay in ICU (r = 0.296,P = 0.013), duration of mechanical ventilation (r = 0.385,P = 0.011). Decreased plasma IL-33 level could be found in the survivors (n = 37,F = 7.798,P< 0.01), and its level in non-survivors (n = 3) was increased (F = 37.283,P> 0.05). The area under the ROC curve (AUC) of IL-33 and PCT in ROC curve were 0.821, 0.829. When the cut-off value of IL-33 was 13.79 ng/L, the sensitivity was 74.2%, the specificity was 79.6%; when the cut-off value of PCT was 4.70μg/L, the sensitivity was 87.5%, and the specificity was 81.4%.Conclusions The concentration of IL-33 3 hours after ICU admission was obviously increased in sepsis patients, and it was positively correlated with PCT, therefore it is valuable in the diagnosis of the infection. In addition plasma IL-33 is related to the severity of sepsis. Its trend of change is valuable in predicting the outcome and in distinguishing sepsis from SIRS.

OBJECTIVE:To compare antidiuretic activity of Ootheca Mantidis before and after processing,and to explore the best medicinal part and mechanism of Ootheca Mantidis. METHODS:96 rats were randomly divided into blank group,model group,positive group,Ootheca Mantidis group,Ootheca Mantidis stir-fried with salt group,steamed Ootheca Mantidis group, crude product eggs and egg shell groups,processed product eggs and egg shell groups,with 8 rats in each group,12 groups in to-tal. Except blank group,other groups were given adenine 250 mg/kg,ig,for 4 weeks to induce kidney-yang and diuresis model. From third week,Ootheca Mantidis crude drug group and processed Ootheca Mantidis group were all given relevant medicine 0.11 g(crude drug)/ml i.g,and crude product eggs and egg shell groups and processed product eggs and egg shell groups were given rel-evant medicine,ig,once a day,by mass ratio of eggs to egg shell(cude drug 1∶2.4,salt stir-fried product 1∶1.7,steamed prod-uct 1∶2.1)for consecutive 4 weeks. The urinary volume,body weight,renal index and the serum contents of ADH and ALD were all determined. RESULTS:Compared with blank group,body weight and serum content of ADH and ALD decreased in model group,while renal index and urinary volume increased(P<0.05). After 4 weeks of treatment,compared with model group,body weight and serum content of ADH increased in Ootheca Mantidis groups,while urinary volume and renal index decreased (P<0.05);serum content of ALD increased in treatment groups;there was statistical significance in the serum content of ALD in those groups except Ootheca Mantidis group,Ootheca Mantidis stir-fried with salt group and steamed Ootheca Mantidis group (P<0.05);except for ALD,those index were in descending order of steamed Ootheca Mantidis group>Ootheca Mantidis stir-fried with salt group>Ootheca Mantidis group,and steamed Ootheca Mantidis shell group had best exchange. CONCLUSIONS:The an-tidiuretic activity of Ootheca Mantidis has been enhanced after processing. The egg shell of steamed Ootheca Mantidis is main me-dicinal part. To increase the serum content of ADH might be one of the main mechanism of arresting polyuria.

Female ; HIV Infections ; prevention & control ; transmission ; Humans ; Infant, Newborn ; Infectious Disease Transmission, Vertical

Diagnostic Tests, Routine ; Humans ; Liver Cirrhosis ; diagnosis

Actinomyces ; growth & development ; Coinfection ; Humans ; Maxillary Sinus ; Maxillary Sinusitis ; diagnosis ; microbiology

Objective To analyze and research on the joint effect of depressive symptoms in patients with schizophrenia.Aripiprazole Tablets and Vin Rafa Sin sustained-release tablets.Methods Selected from January 2013 to December 2013 inherent in our hospital and accepted treatment with depression symptom of schizophrenia patients with a total of 82 cases,according to the different treatment would be all of the patients were divided into study group and control group,which group of a total of 44 cases of patients,combined with aripiprazole tablets and the symplectic method sustained-release tablets;control group total 38 cases of patients with aripiprazole tablets.The two groups of patients with treatment time for 10 weeks,in the course of treatment and after treatment of the two groups of patients with curative effect comparison.Results After treatment for 6 weeks,the HAMD scores in treatment group was significantly lower than that in control group (P ＜ 0.05).The cured rate and the total effective rate in treatment group were significantly higher than those of control group (P ＜ 0.05).Tess score in treatment group was significantly lower than that of control group (P ＜ 0.05).After the treatment of 6 weeks,PANSS scores of the treatment group was significantly less than that of control group (P ＜ 0.05).Conclusions Aripiprazole tablets and venlafaxine hydrochloride sustained-release tablets combined with depressive symptoms of schizophrenia patients and quick can effectively improve the quality of life in patients,and relatively few adverse reactions,good curative effect.It is worthy of popularization and application.

Metabolic syndrome (MS) is characterized by high blood glucose,high blood lipid,high blood pressure,and abdominal obesity with insulin resistance as the main characteristics.At present,some epidemiological surveys demonstrate the incidence rates of some malignant neoplasms including prostate cancer,hepatocellular carcinoma,colorectal adenoma,and breast cancer among MS patients were obviously increasing.Patients with MS present a high morbidity of certain tumors.This article summarizes the relationship between MS and malignant neoplasms as well as related mechanisms.

BACKGROUND:Coronary drug-eluting stent implantation is the best treatment for acute ST-segment elevation myocardial infarction, but because of many combined risk factors in elderly patients, the mortality rate is increased with aging. OBJECTIVE:To analyze whether age differences is a significant independent predictor of in-hospital clinical outcomes in patients with acute ST-elevation myocardial infarction treated with domestic rapamycin drug-eluting stent implantation. METHODS:A retrospective analysis was done in 307 patients with first acute ST-elevation myocardial infarction treated with emergent rapamycin drug-eluting stent implantation for primary percutaneous coronary intervention. According to the age, there were three groups: non-elderly group (< 65 years old,n=175), low-elderly group (65-74 years old,n=83), and high-elderly group (≥ 75 years old,n=49). Clinical features, mortality, cardiac events during in-hospital stay were compared among three groups. RESULTS AND CONCLUSION: Compared with the non-elderly group, the proportion of female patients and the history of hypertension were higher in the low-elderly or high-elderly group (P < 0.05). The history of smoking was higher in the non-elderly group than the other groups (P < 0.05). The incidences of Kilip classification≥II, three-lesion or left main coronary artery disease were higher in the high-elderly group than the other groups (P < 0.05). Hemoglobin, total cholesterol, triglycerides, low-density lipoprotein, and cholesterol levels were higher in the non-elderly group than the other groups (P < 0.05). Serum creatinine level, incidence of malignant arrhythmia and incidence of in-hospital cardiac death were increased with aging. Successful reperfusion was higher in the high-elderly group than in the low-elderly or non-elderly group. Rate of acute heart failure and combined cardiovascular events were higher in the high-elderly group than in the low-elderly or non-elderly group (P < 0.05). Multivariate logistic regression analysis show that age is an independent risk factor for in-hospital mortality in patients with acute ST-elevation myocardial infarction treated with rapamycin drug-eluting stent implantation.

BACKGROUND:Studies have reported that underin vitro experimental environment, antisense endothelin nucleic acid nanometer carrier can express target nucleic acid and produce RNA interference effect after enfolding by 12-alkylated chitosan nanoparticles, which can effectively inhibit the excessive generation of endothelin from inflammatory cytokines induced by alergen. OBJECTIVE:To investigate the effect of 12-alkylated chitosan nanoparticles enfolding antisense endothelin converting enzyme RNA expression plasmid on airway hyperresponsiveness in asthmatic mouse models. METHODS: Forty Balb/c mice were randomly divided into four groups: control, chitosan nanoparticles, normal saline and plasmid groups. Mice in the chitosan nanoparticles, normal saline and plasmid groups were subjected to sensitization by an intraperitoneal injection with ovalbumin (0, 14 days) and motivation by aerosol inhalation of ovalbumin (24, 25, 26 days) to induce asthma models. Mice in the control group were subjected to sensitization and motivation by the perfusion of normal saline. At 24hours before the first excitation, mice in the control, chitosan nanoparticles, normal saline and plasmid groups were perfused with normal salinevia airway, 12-alkylated chitosan nanoparticles, normal saline and antisense endothelin converting enzyme RNA. At 48 hours after the last excitation, the airway reactivity of mice was detected. After 28 days, bronchoalveolar lavage fluid cytology, lung histopathology, cytokines in spleen cel culture supernatant were detected. RESULTS AND CONCLUSION:Compared with the control group, the total number of plasmid cels, the percentage of eosinophils, eosinophil absolute counts, interleukin-4 levels, endothelin levels and airway hyperresponsiveness of mice in chitosan nanoparticles, normal saline and plasmid groups were increased (P < 0.05), and the lung inflammation was more severe. These indicators in the chitosan nanoparticles group were al lower than those in the normal saline and plasmid groups (P < 0.05) and the degree of inflammation was lighter than that in the saline and plasmid groups. These results demonstrate that 12-alkylated chitosan nanoparticles enfolding antisense endothelin converting enzyme RNA expression plasmid can reduce the synthetic amount of asthma endothelin and inhibit airway responsiveness.

BACKGROUND:Sagittal split ramus osteotomy is one of the most popular surgical procedures for correcting various mandibular deformities. There have been numerous modifications to this technique on the basis of clinical experiences and observation, but relevant anatomical data are very limited. A lot of anatomic studies on the mandible have been performed during the recent 20 years. OBJECTIVE:To entirely understanding the morphology of the mandibular ramus in the sagittal split ramus osteotomy and to explore the research progress in the sagittal split ramus osteotomy. METHODS:An electronic search of CMB and Medline was performed for relevant reviews and papers published from 1991 to 2011. Progress in the sagittal split ramus osteotomy was also analyzed. RESULTS AND CONCLUSION:Total y 26 articles related to the sagittal split ramus osteotomy were enrol ed. The keys to avoiding complications due to the sagittal split ramus osteotomy are as fol ows:distribution of cancel ous bone in the mandibular uvula area, distribution of cancel ous bone between the mandibular canal and buccal cortical bone during splitting progress, as wel as location and trend of the mandibular canal. Pre-operative CT test contributes to a successful sagittal split ramus osteotomy and a reduction in complications, and the anatomical measurement can be applied clinical y to a greater extent.