Objective To observe the auto-limbal epithelium and amniotic membrance transplantation in treatment of Symblepharon.Methods Transplant with auto-limbal epithelium and aminiotie membrance including 23 patients (31eyes) some of them are recurrentcd pterygium,most of them are symblepharons.[ Result ] In the followup period for 3～12 months,Ocular surface reconstruction was successful in 26 of 31 eyes with symb|epharon,but five of them recurrented three months later with lees than pre-transplantation in degree.Conclusion The transplantation with auto-limbal epithelium and membrance transplantation is an effective method for the symblepharon.

Objective To investigate the clinical efficacy of cobamamide combined with Longxuejie in the treatment of herpes zoster in the elderly .Methods 104 elderly patients with herpes zoster were randomly divided into the treatment group and the control group .Both the two groups were given orally famciclovir 250mg,3 times a day for 14 days,intramuscularly injected gland glycosides cobalamin 500mg,1 day,14 days a course of treatment ,the treat-ment group was given Longxuejie enteric-coated tablets 4,3 times a day orally for 14 days.The blisters disappeared , pain relief,and recovery time of completely crusted ,treatment efficacy and neuralgia were compared between the two groups.Results In the treatment group,34 cases were cured,effective in 13 cases,total effective rate was 90.4%, which was significantly higher than 73.1% in the control group,the difference was statistically significant (χ2 =5.216,P<0.05).The blisters disappeared,pain relief,and recovery time of completely crusted in the treatment group were significantly shorter than those in the control group (t =3.216,5.432,4.643,3.654,all P <0.05).No postherpetic neuralgia was observed in the treatment group ,the incidence rate of postherpetic neuralgia of the control group was 13.5%,the difference was statistically significant (χ2 =4.875,P<0.05).Conclusion The Longxuejie combined with cobamamide in the treatment of herpes zoster in elderly patients has reliable effect ,fast improvement of clinical symptoms,a low incidence of postherpetic neuralgia ,it is worthy of clinical application .

OBJECTIVE:To enhancement lawconsciousness and ability of self-protect of pharmacist.METHODS:Analysed the problem which occured in their work from the point of view of law and bring forward some advice.RESULTS &CONCLUSION:Pharmacist should do their work strictly according to the code for avoidance law risk.

Objective To explore the expressions and significances of sICAM-1 and sCD44v6 in peritoneal lavage fluid after laparoscopically assisted radical gastrectomy,in order to evaluate the risk of laparoscopically assisted radical gastrectomy for the peritoneal plant transfer.Methods Ninety-two patients with gastric cancer were randomly divided into the control group and the experimental group.The control group was treated with open laparotomy and the experimental group was treated with laparoscopic surgery.The concentrations of both sICAM-1 and sCD44v6 were detected by enzyme linked immunosorbent assay.The positive rates of tumor cells in the two groups were compared.Results The expressions of sICAM-1,sCD44v6 in the two groups before operation had no significant difference (t =0.198,P =0.543 ; t =0.897,P =0.429).After operation,sICAM-1 and sCD44v6 levels increased significantly,but there was no significant difference between the two groups (t =0.766,P =0.312 ; t =1.092,P =0.129).The positive rates of tumor cells in the two groups before and after operation have no significant differences (x2 =0.789,P =0.287 ; x2 =0.912,P =0.198).Conclusion Laparoscopically assisted radical gastrectomy does not increase the expression of sICAM-1 and sCD44v6 in peritoneal lavage fluid and the risk of peritoneal metastasis.It is worthy of clinical poptdarization and application.

Objective To study the effect of acid burn on rat corneal endothelial damage and its repair.Methods The model of mild,moderate and severe acid burn in cornea was established in 90 Long-Evans rats(n=30 for each degree of acid burn) with 4 rats served as normal controls.Corneal edema,opacity and the healing of epithelium were observed with a slit lamp microscope.The corneal endothelium from half rats of each degree acid burn was stained with trypan blue and alizarin red,and that from the other half with hematoxylin and eosin(HE) immediately,30 min,1,3,7,14,21,28 d after cautery.The endothelial damage and its repair were observed and the number of the leucocytes in cornea was calculated under light microscope.Results No damage was seen in the corneal endothelial cells of mild,moderate acid burn,only a few leucocytes could be seen in the cornea,and the healing of epithelium,the regression of edema were within 7 d.The endothelial cells necrosed and shedded in the wound area by severe acid burn.The endothelial cells in the margin were deformed,elongated and slided to the damaged area during repair,and a great number of leucocytes infiltrated,the healing of corneal epithelium was delayed,the regression of edema was up to 28 d.Conclusion The corneal endothelial cells were damaged in severe acid burn.The secondary inflammatory reaction took part in the damage of corneal endothelial cells after severe acid burn.The damage of endothelial cells delayed the healing of corneal ulcer and edema.

This paper introduces primarily the design of hardware and software systems in infrared automatic handwash basin including the working process of the electric circuit. At the same time, in order not to act mistakenly suffering from exterior interference, the anti-interference function is added to the software, which is also the design technique of this software.

Artificial hip replacement is an effective therapy to eliminate hip pain,correct deformity and restore function.With population aging and improving of people life quality,artificial hip replacement has been widely conducted.Over the past 40 years,artificial hip replacement technique has rapidly developed,and minimally invasive technology presents development direction of future artificial hip replacement.Skilled operation experience and instrument application are two key factors to ensure surgery success.The minimally invasive technology arouses more attention from clinical doctors and patients due to minimal invasion,little blood loss and fast recovery.However,not all patients can undergo minimally invasive hip replacement.This paper summarized related studies on minimally invasive hip replacement in domestic,and discussed the indications,surgical approach,complications and prevention.

Objective:To study the clinical value of susceptibility weighted imaging(SWI)in brain diseases.Methods:37 cases with blood vessel pathological change of brain diseases were collected and analyzed,including 8 example of angioma cavernosum,10 cerebral infarction,15 brain tumor,2 venous malformation and 2 venous sinus thrombus.Conventional T1WI,T2WI,DWI,the SWI and enhanced T1WI,MRA images were performed to compare the superiority of the SWI images to the others in demonstrating the small hemorrhagic focuses,the small vein,hemosiderin,calcification and other paramagnetic materials.Results:SWI could distinguish the hemorrhage from the blood vessel and discover the smaller hemorrhagic focuses of the angioma cavernosum.It could discover more tiny veins adjacent to the dilated draining vein.SWI could detect small hemorrhages in acute cerebral infarction and small draining veins in brain tumor.And draining veins in deep part and expanded rete venosum in the surfaces of brains could be obviously demonstrated when sinus venosus thrombus occurred.Conclusion:SWI is a new pulse sequence that can demonstrate the abnormal low current capacity blood vessels,the structure of small vein,the tiny hemorrhages as well as ferrugination and calcium deposition sensitively.So it can be applied in the diagnosis and antidiastole of the central nervous system diseases as an important supplement to the MRI conventional sequences.

AIM: To study the mechanism and protective effect of rosmarinic acid from Rosmarinus officinalis extract on acute hepatic injury induced by carbon tetrachloride in mice. METHODS: Mouse model of acute hepatic injury was set up by giving CCl_4 abdominally.The effects on ALT,AST avtivities,TG level in serum and MDA level in liver tissue were examined.Histological change and pathological change were observed by means of hematoxylin and eosin staining.The body weight,liver weight of each mice were measured. RESULTS: Rosmarinic acid significantly reduced the activities of ALT,AST,level of TG in serum and level of MDA in liver tissue,restrained the enlargement of liver and reduced the necrosis in pathological observation. CONCLUSION: Rosmarinic acid shows significant inhibitory effects on liver injury induced by CCl_4 in mice.The mechanisms may be related to its effects of reducing lipid peroxidation product.

Objective:To investigate the influences of different OD value and centrifugal parameters on the ultrastructure of Escherichia coli in order to find out the best OD value,speed and time of centrifugation.Methods:According to orthogonal experimental designing principle,the E.coli with different OD600 values are divided into 9 different groups of centrifuge speed and time.The changes of E.coli morphology in each group at different OD600 value,centrifuge time and speed were compared under transmission electron microscope.Results:The different OD600 values and centrifugal parameters have little influence on the ultrastructure of E.coli,but when the OD600 value at 0.8,centrifuge time at 15 min,the cross-section ratio was significantly higher than other groups and was more suitable for observation.Conclusion:The best parameter to prepare the TEM samples of E.coli is under the OD600 value at 0.8 and centrifugation time at 8 minutes and speed at 5 000～12 000 r/min.