10 kinds of normal and immunodifi-cient mice were used and they were found to have different levels ofNK activity.The NK activity toHuman to Human,Human to Mouseand Mouse to Mouse hybridomacells was much lower thanthat to YAC-1 cells in all mice andthere were no significant differencesbetween the two kinds of human Bcell hybridomas in susceptibility toNK cells.The results showed thatNK cells had no obvious effects onxenografts of human B cell hybri-domas.

The red blood cell sorbitol levels of 23 healthy subjects and 66 diabetic patients were measured enzymatically. Results showed that erythrocyte sorbitol levels of both IDDM (17) and NIDDM (38) patients with poor control were significantly higher than that of the controls and positively correlated with the coincident plasma glucose concentration, 24-hour urinary glucose excretion and HbA1c. There was no significant difference between the red cell sorbitol concentration of NIDDM with normal blood glucose level and that of the normal group; moreover, red cell sorbitol levels did not sig-nificantly correlated with FBG in both groups. Although there was no significant difference between the FBG of poorly controlled IDDM and NIDDM groups, red blood cell sorbitol levels were significantly different in both groups. This suggested that red blood cell sorbitol level could be used as an indicator of diabetic control.

Objective To investigate the clinical efficacy of cobamamide combined with Longxuejie in the treatment of herpes zoster in the elderly .Methods 104 elderly patients with herpes zoster were randomly divided into the treatment group and the control group .Both the two groups were given orally famciclovir 250mg,3 times a day for 14 days,intramuscularly injected gland glycosides cobalamin 500mg,1 day,14 days a course of treatment ,the treat-ment group was given Longxuejie enteric-coated tablets 4,3 times a day orally for 14 days.The blisters disappeared , pain relief,and recovery time of completely crusted ,treatment efficacy and neuralgia were compared between the two groups.Results In the treatment group,34 cases were cured,effective in 13 cases,total effective rate was 90.4%, which was significantly higher than 73.1% in the control group,the difference was statistically significant (χ2 =5.216,P<0.05).The blisters disappeared,pain relief,and recovery time of completely crusted in the treatment group were significantly shorter than those in the control group (t =3.216,5.432,4.643,3.654,all P <0.05).No postherpetic neuralgia was observed in the treatment group ,the incidence rate of postherpetic neuralgia of the control group was 13.5%,the difference was statistically significant (χ2 =4.875,P<0.05).Conclusion The Longxuejie combined with cobamamide in the treatment of herpes zoster in elderly patients has reliable effect ,fast improvement of clinical symptoms,a low incidence of postherpetic neuralgia ,it is worthy of clinical application .

Objective:To investigate the influences of different OD value and centrifugal parameters on the ultrastructure of Escherichia coli in order to find out the best OD value,speed and time of centrifugation.Methods:According to orthogonal experimental designing principle,the E.coli with different OD600 values are divided into 9 different groups of centrifuge speed and time.The changes of E.coli morphology in each group at different OD600 value,centrifuge time and speed were compared under transmission electron microscope.Results:The different OD600 values and centrifugal parameters have little influence on the ultrastructure of E.coli,but when the OD600 value at 0.8,centrifuge time at 15 min,the cross-section ratio was significantly higher than other groups and was more suitable for observation.Conclusion:The best parameter to prepare the TEM samples of E.coli is under the OD600 value at 0.8 and centrifugation time at 8 minutes and speed at 5 000～12 000 r/min.

Artificial hip replacement is an effective therapy to eliminate hip pain,correct deformity and restore function.With population aging and improving of people life quality,artificial hip replacement has been widely conducted.Over the past 40 years,artificial hip replacement technique has rapidly developed,and minimally invasive technology presents development direction of future artificial hip replacement.Skilled operation experience and instrument application are two key factors to ensure surgery success.The minimally invasive technology arouses more attention from clinical doctors and patients due to minimal invasion,little blood loss and fast recovery.However,not all patients can undergo minimally invasive hip replacement.This paper summarized related studies on minimally invasive hip replacement in domestic,and discussed the indications,surgical approach,complications and prevention.

Objective: To investigate the effect of two modes of one-lung ventilation(OLV) on lung lobectomy in pediatric patients.Metheds: Twenty ASA Ⅰ-Ⅱ patients(8 male,12 female) aged 2~14 year,weighing 10~30 kg,and undergoing lung lobectomy were enrolled in this study.The patients were premedicated with intramuscular Sod Luminal 3~5 mg/kg and Atropine 0.01 mg/kg im,30 minutes before induction.Induction was performed with Dorcum 0.1 mg/kg,Fentanyl 5 ?g/kg and Vecuronium 0.1 mg/kg,maintained with Vecuronium 70 ?g/(kg?h) and Propofol 5mg/(kg?h) iv boluses.Ordinary tracheal tube was inserted into bronchus(14right,6 left) during OLV.First,OLV with volume-control mode maintained 25 min,and then,OLV with pressure-control mode was performed.In first step Vt was set at 8~10 ml/kg and respiratory rate was adjusted to maintain PetCO2 between 30~40 mmHg.In second step the inspiratory pressure was set according to the plateau pressure during step 1,hemodynamics and PaO2 were measured and recorded.Results : During OLV with pressure-control mode PaO2 was significantly increased,as compared with that during volume-control mode(P

Objective: To study the expression of Echinococcus multilocularis 14-3-3 antigen encoding gene in Bacillus Calmette-Guerin(BCG).Methods: The recombinant plasmid pBCG-Em14-3-3 was introduced into BCG by electroporation and the bacteria with pBCG-Em14-3-3 were cultured to logarithm growth period,then induced daily by heating at 45℃ for 30min in the last three days.The expression products were analyzed by SDS-PAGE and Western blot.Results: The Em14-3-3 recombinant protein was successfully expressed by pBCG-Em14-3-3 in BCG as an obvious band of 27kDa by SDS-PAGE,which accounted for 11% of total bacterial protein in BCG.Conclusion: The pBCG-Em14-3-3 could be expressed efficiently in BCG and be combined with specific antibody in mice sera,which suggested that the Em14-3-3 recombinant protein expressed by rBCG-Em14-3-3 vaccine was of specific antigenicity.

Objective:To summarize the anesthesia experience of open heart surgery in 43 infants(weigh less than 5kg) and to explore the related issues with myocardioprotection and brain protection.Methods:Conventional induction of anesthesia,nasotracheal intubation,arteriovenous puncture,cardiopulmonary bypass were established,according to the needs for maintaining the body low temperature(deep,middle,superficial);haemodynamics was maintained with vasoactive agent after aorta was opened.Results:Two infants with transposition of conducting arteries succumbed to stoma leak.One infant with tetralogy of Fallot succumbed to low cardiac output syndrome,bellows complication appeared in 8 cases,Ⅲ.atrial ventricular block 1,and the rest infants recovered well.Mortality was 6.98%in this group.Conclusion:Cardiac anesthesia control in infants with low weight is difficult,death rate is hiqh and complication is complex.Butas long as control is done well,mortality omd complitation can be devreased.

Objective:To study the status quo of quality of life and its influencing factors among Junior High School Students of Three-Gorges Reservoir and Non-Three-Gorges Reservoir in Chongqing,and to provide basis for students' QOL improvement.Methods:672 Three-Gorges Reservoir students and 630 Non-Three-Gorges Reservoir students were tested by using the Quality of Life Scale for Junior High School Students(JHSS-QOLS).Multiple stepwise regression analysis was utilized to study the major factors affecting QOL of students in Chongqing.Results:The quality of life of junior high school students in Three-Gorges Reservoir was higher than that of students in Non-Three-Gorges Reservoir.Physical exercises,sleep,chronic disease,parental rearing style were important influencing factors in quality of life of students.Conclusions:The key steps of improving the quality of life of Junior High School Students should include reducing the learning load of students,the prevention and control of chronic disease and the improvement of parental educational level.

This paper introduces primarily the design of hardware and software systems in infrared automatic handwash basin including the working process of the electric circuit. At the same time, in order not to act mistakenly suffering from exterior interference, the anti-interference function is added to the software, which is also the design technique of this software.