Blood Circulation ; Carbon Dioxide ; blood ; Humans ; Medicine, Chinese Traditional ; Meridians ; Qi ; Science

To appraise the preventive and improvable effects of different movements on OA joints,animal models by fixing the left posterior knee joint in extention using plaster cast for 8 weeks in Newzealand rabbits were carried out. The rabbits were randomly divided into different groups, and the X-RAY、range of motion and ligament biomechanical features of the joint were observed.The results showed that proper movement improved the range of motion, restored tensile strength of the ligament around the OA joint.It is evident that movement improves the function of the OA joint and delays the OA exacerbation.The combination of active and passive movement is the best way to achieve good result.

Objective To explore the curative effect of acupuncture and ultrasonic therapy on treatment of the menopausal osteo -porosis patients who suffered from pain in waist and back .Methods 120 cases menopausal osteoporosis patients from outpatients were divided into 2 groups randomly .Acupuncture and ultrasonic therapy were used in the treatment group ,soybean isoflavone was only supplied in the control group .Then pain intension and bone density in lumbar vertebrae were observed .Results Compared with control group ,the pain in treatment group relieved more effective (P< 0 .05) .The bone density in treatment group was higher than that in control group (P< 0 .05) .The efficient of treatment group is higher than control group (P < 0 .05) .Conclusion The method of acupuncture and ultrasonic therapy can improve the bone density and relieve pain .It can treat the menopausal osteoporo-sis effectively .

Objective To explore the relationship of TCM syndromes in chronic heart failure (CHF) with left ventricular mass index (LVMI) and left ventricular ejection fraction (LVEF). Methods Two hundred and ninety-six CHF patients were classified into the syndromes of Qi deficiency (21 patients), Qi deficiency and blood stasis (98), fluid retention due to Qi deficiency and blood stasis (72), blood stasis due to the deficiency of Qi and yang (56), and blood stasis and fluid retention due to the deficiency of Qi and yang (49). LVMI and LVEF in different syndromes were campared.Results LVMI presented an increasing sequence and LVEF presented a decreasing sequence in the syndromes of Qi deficiency, Qi deficiency and blood stasis, fluid retention due to Qi deficiency and blood stasis, blood stasis due to the deficiency of Qi and yang, and blood stasis and fluid retention due to the deficiency of Qi and yang. Between the syndromes of Qi deficiency, Qi deficiency and blood stasis, and fluid retention due to Qi deficiency and blood stasis, there were significant differences of LVMI and LVEF (P0.05), but LVMI and LVEF in these two syndromes differed from those in the former three syndromes (P

Objective:To observe autophagy in human peripheral blood T lymphocyte.Methods:Peripheral blood T lymphocytes of healthy adults were separated with Percoll(1.073 g/ml) and harvested by using nylon column. The cultured cells were divided into control and dexamethasone(DXM) group, and cell morphology was observed through light microscope, electron microscope and fluorescent microscope at 0 and 72 h. And incidence rate of autophagy was analyzed by flow cytometry.Results:①The natural cultured T lymphocytes showed typical morphology of autophagy. ②There was significant statistic difference between 0 and 72 h incidence rate of autophagic T lymphocyte in both control and DXM group. ③There was significant statistic difference in 72 h incidence rate of autophagic T lymphocyte between control and DXM group.Conclusion:Autophagy can be seen in human peripheral blood T lymphocytes, and DXM could induce autophagy.

Objective To explore the relationship between the clinical characteristics and laboratory examinations.Methods Retrospective analysis was performed on the clinical data in 172 patients diagnosed as infectious mononucleosis(IM)in our department recently.Results The onset rate was high from 3 to7 years old.The main clinical presentations included fever,neck lymphadenopathy,pharyngitis,hepatomegaly,and splenomegaly.Some patients accompanied with obvious sonorous.The occurring rate of the complications was 74.4%,especially deteriorated liver function was the most common,next came to the lung infection.The proportion of atypical lymphocytes raised in 73.3% cases.The positive rate of EBV-VCA-IGM was 65.1%.Myocardium ferment chart went up in 27.9% cases,Erythrocyte sedimentation rate increased fast in 61.6%,CRP gently got higher in 62.8%.However,the ratio of atypical lymphocyte and EBV-VCA-IGM positive or negative had little to do with the pathologic condition and complications in IM.Conclusions The clinical progress of most IM is benign and multiple organs can be involved with atypical clinical manifestations.Examination for atypical lymphocytes is an easy and effective means to screen IM.

Objective To research the methods of treating gynecological inflammatory diseases with TCM. Methods By analyzing gynecological etiology and pathogenesis of inflammatory diseases, we explored the functions of pathogenic dampness, heat, blood stasis, and deficiency of genuine Qi on the onset, development and changes of gynecological inflammatory diseases. Results Gynecological inflammatory diseases is mainly caused by external invasion of pathogenic dampness, heat and insect poison and internal disorder of viscera, leading to the generation of internal dampness, heat, blood stasis and turbid fluid. Besides, the deficiency of genuine Qi is also a major reason for this disease. The therapeutic methods should be eliminating dampness, clearing heat, removing blood stasis, and strengthening genuine Qi. Conclusion TCM is effective in treating gynecological inflammatory diseases.

Objective To investigate the effects of electro-acupuncture (EA) combined with repetitive transcranial magnetic stimulation (rTMS) on protein kinase A-cyclic adenosine monophosphate response element binding protein (PKA-CREB) signal transduction system after focal cerebral ischemia in adult rats and to explore the mechanism of EA combined with rTMS in treating ischemic brain injury.Methods The animal model of transient focal ischemia was made by artificial middle cerebral artery occlusion. Seventy-five Wistar rats were randomly divided into normal group, model group, EA group, rTMS group and EA+rTMS group. The expressions of PKA and pCREB in hippocampus were detected and the neurologic impairment rating was observed at the 7th, 14th and 28th days, respectively, after infarction.Results The average gray densities of PKA and pCREB expressions in hippocampus after focal cerebral ischemia in model group were higher at the 7th d, lower at the 28th d than that in normal group (P＜0.05);higher in EA group, rTMS group, EA+rTMS group than that in model group at all time points (P＜0.05), higher in EA+rTMS group than that in EA group and rTMS group at 7th and 14th d(P＜0.05), and there was no difference between EA group and rTMS group(P＞0.05).The improvement of neural motor function was obvious in EA group, rTMS group and EA+rTMS group compared with model group (P＜0.01), especially in EA+rTMS group.Conclusions EA combined with rTMS can promote the functional recovery after cerebral ischemia,enhance the expression of PKA-CREB signal transduction system in hippocampus after focal cerebral ischemia, which might be one of the important mechanisms of EA combined with rTMS in treating ischemia brain injury.

Objective　To investigate whether the p21ras/ERK (MAPK) cascade is involved in regulating the production of TNF-β and IL-2 in Jurkat cells. Methods　Cells were transiently transfected with a dominant negation construct of p21ras (ras15A or ras17N), raf (raf 1-130) or ERK1 (erk1K71R), or transforming p21ras (H-ras61L) or constitutively active raf (raf22W) using lipofectamine. Control cells received a plasmid carrying β-galactosidease. Transfectant cells were stimulated with PMA/PHA and released TNF-β and IL-2 quantitated by ELISA. Results　In control cells, the production of TNF-β and IL-2 was approx. 2 fold by PMA/PHA stimulation (P<0.001), while transfection with a dominant negative mutant construct abrogated the effects of PMA/PHA. Transfection with a constitutively active mutant had no effect on basal production of TNF-β or IL-2, but all of the constitutively active mutants significantly enhanced the response of the cells to PMA/PHA (P<0.05). MEK1 inhibitor PD98059 inhibited the effects of PMA/PHA and active construct genes on ERK activities and cytokine production. Conclusion　These data demonstrate that the p21ras-ERK cascade plays an important role in regulating the production of both TNF-β and IL-2 in Jurkat cells stimulated with PMA/PHA.

Objective Observe the efficacy of the Qudu-lotion for postoperative edema, postoperative analgesia and promoting wound repair. Methods treatment group(60 patients)applying fumigation and washing with the Qudu-lotion twice a day; control group(60 patients)applying fumigation and washing with 1/5000 potassium permanganate solution. Result The efficacy for the edema and pain of treatment group was superior to those of control group. The cure time was(13.54±3.75)days in treatment group and (17.2±4.22) days in control group. Conclusion In the treatment of postoperative edema, postoperative analgesia and promoting wound repair, the Qudu-lotion have more curative effect.