Protein biochips industry has been making significant progress recently. It played a role in the discovery-oriented proteomics, but now the research emphasis turns to the study of important functional proteins. The emergence of the low density protein biochip technique facilitated this study conversion. This technology has advantages of low cross-reaction, high signal intensity and good precision. This paper reviewed various medical and pharmaceutical applications of the low density protein biochips in disease diagnostics and monitoring, drug discovery and testing, as well as molecular interaction and signaling pathways.

Objective:To constract a method of measurement microamount hydrogen sulfide (H_2S) using sensitive sulphur electrode. Methods: According to the physical and chemical characters of H_2S, H_2S, which in the fluids by mean of physical dissolve and chemical shape, is turned to sulphur ion (S 2-) by chemical responses. After the microamount of S 2- was measured by sensitive sulphur electrode, and the concentration of H_2S was converted, a method was constructed to measure the H_2S. It was used to analyze the concentrations of H_2S of plasma in rats and humans, the endogenous concentration of H_2S of cardiovascular tissue in rats, and CSE activity of cardiovascular tissues and cells. Results: The exponential regression of S 2- in the extent including 1 to 80 ?mol/L was found using sensitive sulphur electrod. The H_2S levels of plasma in male and female rats were 40?4 and 41?5 ?mol/L, respectively, and significant difference was not found; those in venous blood plasma of men and women were 33?4 ?mol/L and 35?5 ?mol/L respectively, without significant difference. There were not significant differences in the aortic endogenous levels of H_2S (24?6 and 25?5 nmol/mg pro) and myocardial levels (19?4 and 19?6 nmol/mg protein) between female and male rats. There were no different results of CSE activity in aortal tissue using sensitive sulphur electrode or traditional methods, however, the CSE activity of vascular smooth muscle cells could be accurately measured using sensitive sulphur electrode, which was difficult in using traditional method. Conclusion: The sensitive sulphur electrode assay was fit for the analysis of CSE/H_2S pathway in cardiovascular research.

A strain C4 with ability of bioconversion from steroid RSA to hydrocortisone was selected fron the soil sample. It was identifide as Cuvularia Iunata according its morphological characters of clump and individuals. The optimum culture medium was established by orthogonal experiments :glucose 10g ,soybean powder 10g,water 1000mL, pH6.5. Determined by silica gel chromatography and high-performance liquid chromatography, the bioconversion ratio of hydrocortisone was 34%. At the same tine, the percentage change of RSA,hydrocortisone and other byproducts were measured during the course of bioconversion.

Objective A comparative study of clinical factors and dose-volume histogram (DVH)parameters on the impact of radiation pneumonitis occurred in the three-dimensional conformal radiotherapy of lung cancer (lung target)and mediastinal tumors and esophageal (mediastinal target).Methods Review 83cases lung cancer,mediastinal tumors and esophageal patients,and analyzed with chi-square tests on the correlation of the clinical factors (gender,age,tumor location,stage,chemotherapy) with radiation pneumonitis;have relevance analysis between the DVH parameters of two targets and radiation pneumonitis; lung target and mediastinal target volume DVH parameters compared to the t-test.Results ≥2 radiation pneumonitis was 36.5 ％.≥2 radiation pneumonitis occurred in various clinical factors had no significant effect.DVH parameters of the two targets,V5,V10,V20,V30,whole lung dose (MLD) were significantly related to RP.Two targets of RP patients V5 [(50.9±17.8) ％,(69.9±20.4) ％],V10 [(38.6±15.2) ％,(53.5±18.8) ％] were statistically significant by t-test (t =2.434,P ＜ 0.05),while V20 (t =0.388,P ＞ 0.05),V30 (t =0.005,P ＞ 0.05) and MLD (t =0.138,P ＞0.05) were no significant difference (P ＞ 0.05),so the same results with the two targets of patients without RP obtained.Conclusion In the lung target and mediastinal target of radiotherapy radiation pneumonitis is related with DVH parameters,especially V20,V30 and MLD impact on the occurrence of RP.

Objective To investigate the dynamic expression from gene and protein levels of hypoxia inducible factor-1α(HIF-1α) during the development of hypoxic pulmonary hypertension (HPH) in neonatal rats.Methods A neonatal rat model of HPH was established,normal neonatal rats were enrolled as the control group.At the 3th 、7th、10th and 14th days,we measured the mRVSP through catheterization of right ventricule,collected hearts to figure out the right ventricular hypertrophy index(RVHI),evaluated vascular remodeling by the percentage of medial thickness to outer diameter of the small pulmonary arteries (MT％) and the percentage of medial cross section on area to the total cross section area of the pulmonary small arteries (MA％),observed the expression of HIF-1α by immunochemistry,and measured the expression of HIF-1α in mRNA and protein by RT-PCR and Western blot respectively.Results The mRVSP increased at the 3th day,and sustained until the 14th day (P ＜ 0.01).RVHI MT％ and MA％ increased at the 7th day,and sustained until the 14th day (P ＜0.01).HIF-1α mainly expressed in endothelium and smooth muscle cells in the CHPH group and the HIF-1αmRNA increased significantly on the 3th and 7th days (P ＜ 0.05),and the HIF-1α protein increased significantly on the 7th、10th and 14th days in the CHPH group compared with the control group(P ＜ 0.01).Conclusion The mRVSP increased at the early stage after hypoxic exposure in neonatal rats,followed by vascular remodeling and right ventricular hypertrophy.Both mRNA and protein levels of HIF-1α sustained higher than control group during the vascular remodeling stage,indicating that HIF-1α might be a important factor contributing to the vascular remodeling.

[Summary] During recent years, increasing evidences have indicated that type 2 diabetes mellitus(T2DM) might increase the risk of certain tumors; the process might be not only related with the chronic pathologic status of T2DM such as hyperglycemia, hyperinsulinemia, insulin resistance, dyslipidemia, status of chronic inflammation but also associated with the long-term use of anti-diabetic drugs (i. e. sulfonylureas, biguanides, glitazones, dipeptidyl peptidase-4 inhibitors, glucagon-like peptitide-1 receptor agonists), as well as the use of insulin and insulin analogues. Herewith a system review was made about the recent progress in studying T2DM and tumor risk.

Aim To investigate the effect of melatonin (MT) and pentoxifylline (PTX) on hepatic ischemia reperfusion (I/R) in rat. Methods 128 rats were randomly divided into four groups: control group, MT treated group, PTX treated group and PTX+MT group (combined group). Those operation procedures including occlusion the inflow to the liver 35 minutes, hepatectomy and reperfusion, were underwent by the animals. The following items were investigated: liver function, endothelin 1 (ET 1), malondialdehyde (MDA), superoxide dismutase (SOD), and one week survival. Results The following changes were detected after the liver ischemia: ①Liver function: the increased scopes of ALT and LDH were significantly lower in treatment groups, especially in the combined group than that in control one; ②ET 1: the levers of ET 1 were significantly increased in every group, especially the control group; ③MT function: the increased scopes of MDA and SOD were significantly lower in MT groups (including MT treated and combined groups) than that in others; ④PTX function: the increased scopes of TNF ? were significantly lower in PTX groups (including PTX treated and combined groups) with a higher rate of survival than that in others. Conclusion ①MT could reverse the oxidative damage, but cannot affect the rate of survival; ②PTX can decrease the secretion of cytokine and the one week survival; ③Based on the synergistic effects, the application of MT+PTX may have beneficial effects against the I/R injury to increase the survival.

A strain C4 with ability of bioconversion from steroid RSA to hydrocortisone was selected fron the soil sample. It was identifide as Cuvularia Iunata according its morphological characters of clump and individuals.The optimum culture medium was established by orthogonal experiments:glucose 10g,soybean powder 10g,water 1000mL, pH6.5.Determined by silica gel chromatography and high performance liquid chromatography, the bioconversion ratio of hydrocortisone was 34%.At the same tine, the percentage change of RSA,hydrocortisone and other byproducts were measured during the course of bioconversion.