ObjectiveTo study the optimized security strategy for informing cancer patients with the disease,and let patients be fully informed and even more actively cooperate with doctors while they are under treatment.MethodsGrouped with adversity quotient (AQ) score,these cancer patients were in comparison with those who had been informed by other ways without support strategy,and different groups of patients were estimated in different strategies in accordance with self-rating anxiety scale (SAS) and self-rating depression scale (SDS).Results With certain strategies,high and low AQ score groups' SAS and SDS scores stably restored in 1 day to 1 week,and the differences had statistical significance(P ＜ 0.01 ).The patients were well-informed without any difficulty and actively cooperated with treatment.No related factors led to self-mutilation,suicide and other serious incidents.While the SAS and SDS scores of the control group who were not informed by the strategy were not satisfactorily recovered,and the patients could not actively cooperate with the doctors.Especially being notified after 1 d to 1 week,the SAS and SDS scores had no significant improvement,and the differences had no statistical significance compared with themselves ( P ＞ 0.05 ).It is difficult to avoid the occurrence of serious incidents.Conclusion Based on AQ score,it is safe and effective to inform cancer patients of their disease condition by individual strategies.It not only respects patients rights,but also gains the active cooperation of the patients,and is helpful for treatment with minimizing adverse effects.

Cardiovascular Diseases ; etiology ; Chronic Disease ; Humans ; Hypertrophy, Left Ventricular ; etiology ; Kidney Diseases ; complications ; Risk Factors

BACKGROUND:Occurrence and development of diabetic complications is closely related to the severity of oxidative stress imbalance in the body. OBJECTIVE:To investigate the effect of embryonic stem cel s on oxidative stress response of rats with diabetic nephropathy. METHODS:Primarily cultured rat embryonic stem cel s were observed for cel morphology and surface antigen detection. Sprague-Dawley rats were divided into experimental group (two injections of embryonic stem cel s via the tail vein), model group (injection of the same volume of PBS), and normal control group (with no modeling, intraperitoneal injection of sodium citrate-citrate buffer). In the former two groups, the rats were intraperitoneal y injected sodium citrate-citrate buffer diluted streptozotocin to establish diabetic nephropathy models before treatment. At 5 weeks after the last injection, blood glucose level, renal function indicators (urine protein/urine creatinine, blood urea nitrogen and serum creatinine) were tested in each group;contents of malondialdehyde and protein carbonyl were detected in the kidney;the expression level of superoxide dismutase was detected by western blot assay. RESULTS AND CONCLUSION:The embryonic stem cel s were oval or round, with clear boundary and good refraction, and highly expressed Oct-4 and SSEA-1. Compared with the control group, renal biochemical indicators, malondialdehyde and protein carbonyl contents were significantly increased, while the expression level of superoxide dismutase was decreased dramatical y in the model group and experimental group (P<0.05);compared with the model group, the renal biochemical indicators, malondialdehyde and protein carbonyl contents were dropped significantly in the experimental group, but the expression of superoxide dismutase was significantly rebounded (P<0.05). Taken together, embryonic stem cel s can reverse the occurrence and development of diabetic nephropathy by inhibiting oxidative stress in progress.

Objective To study the significance of serum human epididymis protein 4 ( HE4 ) and human cartilage glycoprotein-39(YKL-40) in the diagnosis of ovarian cancer .Methods 100 patients with ovarian cancer , 90 patients with benign tumor of ovary and 50 healthy people were enrolled .Serum HE4 and YKL-40 were detected . Results The positive rates of serum HE 4 in ovarian cancer group , ovarian benign tumor group and healthy group were 86.0%,5.5%,2.0%.The positive rate of serum HE4 in ovarian carcinoma was significantly higher than that in benign ovarian tumor group and the healthy group (χ2 =31.06,30.82,all P<0.01).The positive rates of serum YKL-40 in ovarian cancer group ,ovarian benign tumor group and healthy group were 82.0%,3.3%,2.0%.The positive rate of serum YKL-40 in ovarian carcinoma was significantly higher than that in benign ovarian tumor group and the healthy group(χ2 =30.92,32.06,all P<0.01).Serum HE4 in the diagnosis of ovarian cancer ,the sensitivity was 86.0%,specificity of 95.7%,accuracy of 91.7%.Serum YKL-40 in the diagnosis of ovarian cancer ,the sensitivity was 82.0%,specificity of 97.1%,accuracy of 90.8%.For the joint detection,the sensitivity for diagnosis of ovarian cancer was 92.0%,and the specificity was 93.6%,accuracy was 95.0%,higher than those of the single detection . Conclusion Combined with serum HE 4 and YKL-40 detection can significantly improve the diagnostic accuracy of ovarian cancer patients .

Objective To investigate the expression of serum vascular endothelial growth factor (VEGF) in children with Wilms tumor,and clarify the effect of VEGF in the invasion and progression of Wilms tumor.Methods Thirty children with Wilms tumor who underwent surgical treatment were retrospectively analyzed as Wilms tumor group,the control group was 30 children who underwent treatment by cold and fever symptoms.The serum VEGF levels of 2 groups were detected by enzyme-linked immunosorbent assay.Results The serum VEGF level of Wilms tumor group was significantly higher than that of control group [(567.90 ± 189.30) ng/L vs.(55.46 ± 18.49) ng/L,t =14.757,P ＜0.05].The expression of serum VEGF was closely related to NWTS stage,prognosis tissue type and lymph node metastasis (P ＜ 0.01).The expressions of serum VEGF in the NWTS Ⅲ-Ⅳ stage,lymph node metastasis and poor prognosis tissue type Wilms tumor were significantly higher than those of NWTS Ⅰ-Ⅱ stage,without lymph node metastasis,good prognosis tissue type Wilms tumor (P＜ 0.01).Conclusions The expression of serum VEGF is high in children with Wilms tumor,the serum VEGF level is closely related to NWTS stage,prognosis tissue type and lymph node metastasis.The high expression of serum VEGF shows the invasion,progression and poor prognosis in children with Wilms tumor.

The whipworm,Trichuris trichiura,has a worldwide distribution but is more common in tropical and subtropical areas and in regions where sanitation is poor.The common name of whipworm is most descriptive,the thick posterior part of the body forming the stock,and the long thin anterior portion.The adult worma are from 3 to 5 cm long,the female worm is longer than the male worm.On the posterior part is pinkish gray and contains the intestine and reproductive organs. Diagnosis is by demonstraion of the characteristic barrel-shaped eggs (50-54 by 22-23μm)usually found in the stool.But their morphology of adult worms and eggs among the human,domestic and wild animals are quite different to diagnosis.The females are larger(35-55 mm long)than the males(30-45 mm).Each female worm can produce 3,000 to 70,000 eggs daily.The eggs are lemon-like with a plug at each end.Their size are 50-54 μm and 22-23 μm respetively.

Leukemia inhibitory factor(LIF) is a multifunctional cytokine.In recent years,investigations have made some advancement in the effect of LIF on central nervous system.LIF shows a high expression in various central nervous system diseases and related animal models,which shows a neuroprotective effect most time in central nervous system injuries.LIF is defined as a neurotrophic factor.It might provide a new strategy for the therapy of central nervous system diseases to undertake further studies on leukemia inhibitory factor and central nervous system diseases.

Objective To evaluate the results of palliative procedures combined with intervention techniques for tetralogy of Fallot with severe hypoplastic pulmonary artery.Methods From December 2002 to Decembre 2009,complete repair of tetralogy of Fallot were performed in 1586 patients including 18 cases ( 12 male and 6 female) with severe hypoplastic pulmonary artery after 8 - 40 (20 + 11 ) month of palliative operations.The patients'age and body weight were 12 - 72 months and 8.0 - 16.5 kg at the time of complete repair.Associated cardiac anomalies include 3 cases of atrial septal defect,2 cases of absence of left pulmonary artery,1 case of the left pulmonary artery arising from the aorta,1 case of left superior vena cava.Major aortopulmonary collateral arteries were confirmed by angiography in 7 patients.Palliative operation was performed once in 13cases,twice in 4 cases and thrird in 1 patient before complete repair.Palliative operations included 14 procedures of Blalock-Taussig shunt,6 of Waterston shunt,3 of right ventricle outflow tract reconstruction and 3 of balloon pulmonary valvuloplasty.Major aortopulmonary collateral arteries were ligated in 4 cases,unifocalized in 1 and interventional embolized in 2 before complete repair.Results There was no early death.One reoperation was performed because of the obstruction of vascular prosthesis in the next day of shunt operation.Nakata index [ (84 ±40) versus (200 ±81 )] and McGoon ratio were increased after complete repair as compared with before operation [ ( 1.14 + 0.33 ) versus ( 1.77 + 0.51 ),P ＜ 0.001 ].Hemoglobin decreased from ( 175 ±46) g/L to ( 149 ± 15 ) g/L (P ＜0.05) and peripheral oxygen saturation increased from (71 ±9)％ to ( 86 ± 5 ) ％ ( P ＜ 0.05 ).Complete repair were performed in all 18 patients eventually.Conclusion Palliative procedures combined with intervention techniques may ameliorate the growth of pulmonary artery effectively and improve the results of complete repair of tetralogy of Fallot with severe hypoplastic pulmonarv arterv.

As a member of homeobox gene family,HOXA7 is a main gene to control hematopoietie cell proliferation and differentiation.The expression and function of HOXA7 in leukemia ale regulated by many upstream elements.and affected by synergism molecules and other homeobox genes. It can induce leukemia through acting on downstream target genes.HOXA7 affects the clinical manifestation of leukemia and overexpression is closely associated with poor therapeutic reaction and prognosis.