Objective To analyze the relative factors of bone marrow suppression after chemotherapy with different-dose carboplatin and paclitaxel (TC) on the patients with ovarian cancer.Methods Sixty-two patients with ovarian cancer admitted in Beijing Obstetrics and Gynecology Hospital from January 2002 to December 2007, using TC regimen ,a total of 196 cycles of chemotherapy ,were divided into two groups by the doses of carboplatin [area under concentration-time curve(AUC) 4 -6 for low-dose,AUC >6 -7 for hight-dose, the carboplatin dose calculated with AUC] or by the doses of paclitaxel (135 -< 150 mg/m2 low-dose,150 - 175 mg/m2 hight-dose). After each TC cycle, the routine blood was test to determine the graduation of the marrow suppression, and then the correlation factors were analyzed with logistic regression. Results (1) The occurrence rate of bone marrow suppression:there were 159 cycles (81.1%) grade 0 - Ⅱ bone marrow suppression, while 37 cycles (18.9%) of grade Ⅲ - Ⅳ. (2) Factors related to bone marrow suppression:the results shown that there were not related to bone marrow suppression,which incluced cellular differentiation, tumor type, height, weight and paclitaxel dose(P>0.05). While,the different cycle, age, the later stages of tumor, serum creatinine concentration, endogenous creatinine clearance rate, AUC values were the relative factors of bone marrow suppression(P =0.000,0.000,0.018,0.033,0.001,0.000). Seven variables were conducted into the logistic regression and the results shown that the different cycles, the age, AUC grades were independent risk factors (P = 0.030,0.043,0.009).(3) When low-dose of paclitaxel was given, the occurrence of bone marrow suppression was related to the carboplatin dose AUC. The higher AUC values for carbopaltin were chosen, the higher of severe bone marrow suppression would happen. (4/14 vs 0,P=0.015). When the dose of high grade of paclitaxel was given, the occurrence of bone marrow suppression in cases with hight-dose carboplatin was statistically significant than that in cases treated with low-dose carboplatin [45.7%(16/35) vs 13.7% (17/124), P=0.000]. Conclusions The independent risk factors of myelosuppression after chemotherapy with TC regime on the patients with ovarian cancer including the cycles, age and AUC values. The carboplatin dose calculating with AUC is related to the occurrence of bone marrow suppression, the higher AUC values for carbopaltin would chosen,the higher of severe bone marrow suppression would be happen.

ObjectiveTo explore the ability of inhibition resistibility of multiple displacement amplification (MDA)in samples with inhibitors. To explain the application and value of MDA in forensic medicine by comparing with using magnetic beads methods(MBM)to purify sample.MethodsDifferent concentra-tions of hemoglobin and humid acid(HA)mixed with DNA samples and then divided the samples into MDA group, MBM group and control group.D3S1358locus was amplified and detected by polyacry-lamide gel electrophoresis detection system and AmpF?STR? IdentifilerTM Plus Kit-capillary electrophore-sis detection system.ResultsWhen hemoglobin concentrations exceed 1 ng/μL or HA concentrations ex-ceed 0.1 ng/μL, amplification products could not be obtained by single-locus system in control group. When hemoglobin concentration exceeds 100 ng/μL or HA concentrations exceed 1 ng/μL, the samples could not be amplified by MBM. Inhibitors in different concentrations were amplified successfully in MDA group without any influence from inhibitors.ConclusionMDA has the capability to remove the inhibi-tion of hemoglobin and HA, which is better than MBM and has a certain value in forensic practices.

Objective To observe the efficacy and side effect of olanzapine combined with tropisetron, dexamethasone for the pre-vention of highly emetogenic chemotherapy-induced nausea and vomiting ( CINV) . Methods A total of 78 patients with highly emetogen-ic single day chemotherapy were randomly divided into two groups:trial group ( olanzapine combined with tropisetron, dexamethasone n=40) and control group (Tropisetron Combined with Dexamethasone n=38). The control of acute CINV, delayed CINV and adverse reac-tions were observed. Results The control rates of acute vomiting in trial group and control group were 87. 5% vs 81. 6%, 65. 0% vs 57. 9% in acute nausea, 75. 0% vs 52. 6% in delayed vomiting, 32. 5% vs 13. 2% in delayed nausea. The trial group was better than the control group in delayed vomiting and delayed nausea which there was difference between them (P < 0. 05). There was no statistical differ-ence between the two groups in the incidence of adverse reactions (P>0. 05). Conclusion Olanzapine combined with tropisetron, dexa-methasone for the prevention was significantly better than tropisetron combined with dexamethasone in the control of delayed CINV in pa-tients received highly emetogenic chemotherapy.

Female ; Humans ; Melanosis ; Middle Aged ; Occupational Diseases

Child ; China ; Humans ; Pediatrics ; Periodicals as Topic ; standards ; statistics & numerical data ; Publishing ; standards ; statistics & numerical data ; Quality Control

Bartter Syndrome ; complications ; Diarrhea ; etiology ; Female ; Humans ; Infant ; Male ; Syndrome

BACKGROUND:Mesencephalon-derived neural stem cells(NSCs)differentiate into dopaminergic neurons in hypoxia and transgenic condition with body culture morphology and function of adequate maturity of dopamine neurons.Mesencephalon-derived NSCs become ideal seed cells for the treatment of Parkinson's disease with the rapid development of stem cell transplantation in the past few years.OBJECTIVE:To review research progress of mesencephalon-derived NSCs transgene for treating Parkinson's disease.METHODS:PubMed database was retrieved in computer(1992-01/2006-12),with the key words of "Neural Stem Cell(NSC),Mesencephalic Neural Stem Cell(M-NSC),Neural Stem Cell Transplant,Parkinson's Disease(PD)".Simultaneously,China Journal Full-Text Database(2003-01/2008-12)was retrieved with the same key words.RESULTS AND CONCLUSION:The NSCs and in the basic research of M-NSCs and M-NSCs transplantation in the treatment of PD-related documents were selected.A total of 83 documents were collected.Following reading titles and abstracts,14 studies with irrelative objectives and contents,16 studies with repetitive contents and 53 articles of Meta analysis were excluded.Totally 47 literatures were included.The main pathologic changes of Parkinson's disease are a dopaminergic neuron degeneration of necrosis in the brain substantia nigra.Using genetically modified neural stem cells in treatment of Parkinson's disease is the most recognized as the most promising treatment.In the hypoxia conditions,transgenic culture of brain-derived neural stem cells can be effectively induced to differentiate into dopaminergic neurons,provide experimental basis for in vitro sufficient amount of dopamine neuron transplantation in the treatment of PD.Basic research of neural stem cells have achieved certain results,but clinical and experimental study of neural stem cell transplantation in the treatment of PD is still stuck in the laboratory stage.Mesencephalic neural stem cell biological characteristics,isolation,proliferation and differentiation are subject to further study.In addition,biological safety problems using mesencephalic neural stem cells in the human body gradually attracted the attention of the people.In vitro neural stem cells of many passages would occur,and will bring certain side effects,and tumorigenicity remains in further exploration.

Objective To explore the effects of lipoteichoic acid(LTA)on phagocytosis of peritoneal maerophages and NO production in vitro.Methods The peritoneal macrophages that were isolated sterilely from Kunming mice were divided into two groups:co-culture with RPMI 1640,and pretreatment with dexamethasone(DEX).Then,the LTA isolated from BFA with different final concentrations(5,10,20 and 40 ug/ml) was added.After the peritoneal macrophages were cultured for 4 and 24 h,the effects of LTA on cytophagocytesis and NO production in vitro were determined respectively by using neutral red and NO assay kit.Results LTA with final concentrations of 5,10,20 and 40/μg/mL significantly promoted the phagocytosis of peritoneal macrophages and production of NO(P<0.05).It also effectively antagonized the inhibitory response of DEX against peritoneal macrophages(P<0.05).The higher concentration of LTA,the stronger effect.Conclusion Not only can LTA stimulate and activate the peritoneal macrophages significantly,but also antagonize the inhibition from DEX in vitro.

Child ; Child, Preschool ; China ; epidemiology ; Evidence-Based Medicine ; Humans ; Kidney Diseases ; diagnosis ; epidemiology ; therapy ; Practice Guidelines as Topic

Neoadjuvant chemoradiotherapy (CRT) has become a standard treatment of locally advanced rectal carcinomas. There is a close correlation between histopathological regression after preoperative CRT and prognosis. To identify the markers for histopathological regression is significant for clinical treatment. Different strategies of chemoradiotherapy, application of targeted drugs in neoadjuvant chemothercepy and internal from neoodjuvant to operation are correlated with histopathological regression.