1.Application of Multiple Displacement Amplification in Samples with Inhibitors
Journal of Forensic Medicine 2016;32(5):342-345
ObjectiveTo explore the ability of inhibition resistibility of multiple displacement amplification (MDA)in samples with inhibitors. To explain the application and value of MDA in forensic medicine by comparing with using magnetic beads methods(MBM)to purify sample.MethodsDifferent concentra-tions of hemoglobin and humid acid(HA)mixed with DNA samples and then divided the samples into MDA group, MBM group and control group.D3S1358locus was amplified and detected by polyacry-lamide gel electrophoresis detection system and AmpF?STR? IdentifilerTM Plus Kit-capillary electrophore-sis detection system.ResultsWhen hemoglobin concentrations exceed 1 ng/μL or HA concentrations ex-ceed 0.1 ng/μL, amplification products could not be obtained by single-locus system in control group. When hemoglobin concentration exceeds 100 ng/μL or HA concentrations exceed 1 ng/μL, the samples could not be amplified by MBM. Inhibitors in different concentrations were amplified successfully in MDA group without any influence from inhibitors.ConclusionMDA has the capability to remove the inhibi-tion of hemoglobin and HA, which is better than MBM and has a certain value in forensic practices.
2.Analysis of relative factors of bone marrow suppression after chemotherapy with carboplatin and paclitaxel on the patients with ovarian cancer
Chinese Journal of Obstetrics and Gynecology 2011;46(3):188-192
Objective To analyze the relative factors of bone marrow suppression after chemotherapy with different-dose carboplatin and paclitaxel (TC) on the patients with ovarian cancer.Methods Sixty-two patients with ovarian cancer admitted in Beijing Obstetrics and Gynecology Hospital from January 2002 to December 2007, using TC regimen ,a total of 196 cycles of chemotherapy ,were divided into two groups by the doses of carboplatin [area under concentration-time curve(AUC) 4 -6 for low-dose,AUC >6 -7 for hight-dose, the carboplatin dose calculated with AUC] or by the doses of paclitaxel (135 -< 150 mg/m2 low-dose,150 - 175 mg/m2 hight-dose). After each TC cycle, the routine blood was test to determine the graduation of the marrow suppression, and then the correlation factors were analyzed with logistic regression. Results (1) The occurrence rate of bone marrow suppression:there were 159 cycles (81.1%) grade 0 - Ⅱ bone marrow suppression, while 37 cycles (18.9%) of grade Ⅲ - Ⅳ. (2) Factors related to bone marrow suppression:the results shown that there were not related to bone marrow suppression,which incluced cellular differentiation, tumor type, height, weight and paclitaxel dose(P>0.05). While,the different cycle, age, the later stages of tumor, serum creatinine concentration, endogenous creatinine clearance rate, AUC values were the relative factors of bone marrow suppression(P =0.000,0.000,0.018,0.033,0.001,0.000). Seven variables were conducted into the logistic regression and the results shown that the different cycles, the age, AUC grades were independent risk factors (P = 0.030,0.043,0.009).(3) When low-dose of paclitaxel was given, the occurrence of bone marrow suppression was related to the carboplatin dose AUC. The higher AUC values for carbopaltin were chosen, the higher of severe bone marrow suppression would happen. (4/14 vs 0,P=0.015). When the dose of high grade of paclitaxel was given, the occurrence of bone marrow suppression in cases with hight-dose carboplatin was statistically significant than that in cases treated with low-dose carboplatin [45.7%(16/35) vs 13.7% (17/124), P=0.000]. Conclusions The independent risk factors of myelosuppression after chemotherapy with TC regime on the patients with ovarian cancer including the cycles, age and AUC values. The carboplatin dose calculating with AUC is related to the occurrence of bone marrow suppression, the higher AUC values for carbopaltin would chosen,the higher of severe bone marrow suppression would be happen.
3.Efficacy of Olanzapine Combined with Tropisetron, Dexamethasone for the Prevention of Highly Emetogenic Chemotherapy-induced Nausea and Vomiting
Journal of Medical Research 2015;44(5):143-146
Objective To observe the efficacy and side effect of olanzapine combined with tropisetron, dexamethasone for the pre-vention of highly emetogenic chemotherapy-induced nausea and vomiting ( CINV) . Methods A total of 78 patients with highly emetogen-ic single day chemotherapy were randomly divided into two groups:trial group ( olanzapine combined with tropisetron, dexamethasone n=40) and control group (Tropisetron Combined with Dexamethasone n=38). The control of acute CINV, delayed CINV and adverse reac-tions were observed. Results The control rates of acute vomiting in trial group and control group were 87. 5% vs 81. 6%, 65. 0% vs 57. 9% in acute nausea, 75. 0% vs 52. 6% in delayed vomiting, 32. 5% vs 13. 2% in delayed nausea. The trial group was better than the control group in delayed vomiting and delayed nausea which there was difference between them (P < 0. 05). There was no statistical differ-ence between the two groups in the incidence of adverse reactions (P>0. 05). Conclusion Olanzapine combined with tropisetron, dexa-methasone for the prevention was significantly better than tropisetron combined with dexamethasone in the control of delayed CINV in pa-tients received highly emetogenic chemotherapy.
4.Study on In Vitro-In Vivo Correlation of Naftopidil Sustained-release Capsules
China Pharmacy 2001;0(07):-
OBJECTIVE:To study the in vitro-in vivo correlation of sustained-release naftopidil capsule,review the rationality of the designed dissolution conditions METHODS:Artificial gastric juice was selected as the dissolution medium,the dissolution rate of the two naftopidil sustained-release capsules were studied;the 3p97 pharmacokinetic program was used to characterize the in vivo course of the sustained-release capsules after giving dogs a oral single dose of 200mg,after this,the percentage absorbed at different time were calculated The input functions were obtained by using deconvolution technique Then the correlation coefficients of the calibration graph by plotting the dissolution rate to the percentage absorbed or the input functions were calculated The in vitro-in vivo correlations of sustained-release naftopidil capsules were studied by comparing the correlation coefficients calculated to the critical correlation coefficients RESULTS:The correlation coefficients calculated by the two methods were both more than the critical coefficients,the in vitro-in vivo correlations of the two sustained-release naftopidil capsules were good CONCLUSION:The two methods mentioned in this paper to study the in vitro-in vivo correlation were simple and reliable,the results of this study were very useful in the rapid formulation screening,the designing of rational dissolution rate conditions,and the providing theory basic of evaluation of the naftopidil sustained-release capsules
5.Research on the determination of cholesterol efflux from foam cells mediated by apoA-1 using HPLC analysis
Chongqing Medicine 2015;(29):4116-4119,4122
Objective To establish a high performance liquid chromatography (HPLC) method for determining the choles‐terol efflux from macrophage‐derived foam cells mediated by apolipoprotein A‐1(apoA‐1) .Methods Human THP‐1 monocytic cells ,pre‐treated with 160 nmol/L phorbol‐12‐myristate acetate (PMA) for 24 h to differentiate into adherence macrophages ,then incubated with 50 μg/mL acetylated low density lipoprotein (ac‐LDL) for 48 h to induce foam cells formation ,then added apoA‐1 for 24 h .THP‐1‐derived macrophage foam cells were identified by oil red O‐staining ,and the cellular cholesterol content by meas‐ured by HPLC method .Cholesterol efflux from macrophage foam cells was determined by HPLC analysis and liquid scintillation counting ,respectively .Results Oil red O‐stainable lipid droplet accumulation were observed in entire cytoplasm of THP‐1‐derived macrophage foam cells .Measuring cellular cholesterol content showed that free cholesterol ,total cholesterol and cholesterol ester content in macrophage foam cells were increased remarkably than PMA group macrophages (P<0 .01) .After treated with ac‐LDL for 48 h ,the macrophage foam cells accumulated 80 .25 μg/mg cell protein and 47 .65 μg/mg cell protein respectively ,and the cho‐lesterol ester accounted for 59 .38% of the cellular total cholesterol (P<0 .01) .The ratio of cholesterol efflux reached 5 .63% and 7 .08% respectively by HPLC analysis and liquid scintillation counting using apoA‐1 mediation (P<0 .01) .Conclusion Combina‐tion of an enzymatic catalysis and HPLC method for determining cholesterol efflux from foam cells is successfully established in this study , thus providing a technical foundation for the further study of cellular lipid homeostasis .
6.The influence of probucol on hs-CRP and Hcy of patients with non-ST-segment elevation acute coronary syndrome
Chinese Journal of Primary Medicine and Pharmacy 2013;20(14):2159-2161
Objective To explore the influence of probucol on high-sensitive C-reactive protein (hs-CRP)and homocysteine(Hcy) of patients with non-ST-segment elevation acute coronary syndrome.Methods 82 patients with non-ST-segment elevation acute coronary syndrome were randomly divided into two groups,the control group (40 cases) and the treatment group (42 cases).The patients in the control group were treated through the conventional treatment,while the patients in the treatment group were treated by probucol on the basis of conventional therapy.They were all treated for three months.Serum levels of hs-CRP and Hcy were detected before and after treatment.Results Serum levels of hs-CRP and Hcy were all decreased after treatment in both two groups.The hs-CRP and Hcy in the treatment group were significantly improved than those of the control group [(0.82 ± 0.43) mg/L vs (1.05 ± 0.52) mg/L,(10.08 ± 7.18) μnol/L vs (15.73 ± 9.16) μmol/L,t =2.1871,3.1168,all P < 0.05].Conclusion Probucol can significantly decrease the serum levels of hs-CRP and Hcy in patients with non-ST-segment elevation acute coronary syndrome.
7.Research progress in Cre recombinase mouse models in ophthalmology
Chinese Journal of Experimental Ophthalmology 2014;32(5):471-475
Cre/LoxP site specific recombinase system is an effective tool for gene recombination in the body to produce specific gene deletion or inversion.The study of gene function is the experimental basis for the development of experimental animal models.Study the Cre/LoxP system,compare the different function characteristics of mouse models for Cre recombinase system in the research of ophthalmology,epecially in the tissue of retina,such as retinal pigment epithelium (RPE) cells,Müller cells and photoreceptor cells,get the Cre transgenic mouse model to establish proper experimental animal models of ophthalmology will lay a solid foundation for the research of the lack of single gene for a particular organization study.Here,the research progress of Cre/LoxP system,the Cre transgenic mouse models and eye specific Cre recombinant enzyme model mice will be summarized.
8.THE VOLAR SUPERFICIAL ARTERIES OF THE HAND IN CHINESE
Acta Anatomica Sinica 1955;0(03):-
A total of 181 upper limbs (73 adults, 108 children) of Chinese were studied. The main results are as follows:1. The types of the superficial volar arch: the ulnar arterial type (U-type) occurred in 36.5?3.58%, the radial-ulnar arterial type (RU-type) in 50.8?3.72%,the median-ulnar arterial type (MU-type) in 10.5+2.28% and the radial-medianulnar type (RMU-type) in 2.2?1.09%. There are obvious differences between adults and children in the U and RU types. The occurrence of U-type in adults is twice as that in children and that of the RU-type is vice versa. We suggest that it might be the result of the pressure of the thenar. The U-type in males is more than that in females, it might result from the same factor.2. The arrangements of the branches of the superficial volar arch are divided into four types: the juxtaposed type occurred in 61.3?3.62%, the common trunk type in 26.0?3.26%, the intervalic type in 8.3?2.05% and the mixed type in 4.4?1.52%. It was found that the common trunks occur more often in the ulnar side, and the intervalic in the radial side of the hand. These, too, might be due to the pressure of the thenar. The number of the branchs of the superficial volar arch is 2~6; among them, the 4-branch type takes major part (53.0?3.71%). The next one is 5-branch type (30.9?3.43%).3. The distribution of arteries derived from the superficial volar arch are divided into two types: the juxtaposed type and the intervalic type occurred in 87.3?2.47% and 12.7?2.47% respectively. The position of the interval is more common in the index finger and the finger artery derived from the superficial volar arch is gradually decreased from the ulnar side to the radial side.
9.ULTRASTRUCTURE OF CARDIOMYOCYTES AND IMMUNOCYTOCHEMICAL LOCALIZATION OF ATRIAL NATRIURETIC PEPTIDE IN RAT ANTERIOR VENAE CAVAE
Acta Anatomica Sinica 1989;0(S1):-
The wall of rat anterior venae cavae has cardiomyocytes running in different directions. These cardiomyocytes were connected by the intercalated discs. Some myocytes in the inner layer of the AVC near the atrium contained specific granules, which were very similar to those in the atrial myocytes in shape and distribution, but the number of the specific granules in the myocytes of venae was much less than that in the myocytes of atrium. Well-developed Golgi complexes, rough surfaced endoplasmic reticulum and multivesicular bodies were frequently found near the specific granules. Immuno-electron microscopy of the specific granules of the venae by using rabbit anti-synthetic rat ANP (1-28) serum, postembedding method and immunogold staining disclosed gold particles on the specific granules of myocytes in the venae were found in the atrial specific granules. It was also shown that the diameter of the labelled granules was somewhat related to the number of the labelling gold particles. No gold particles of the myocytes of the venae when the section was beforehand treated with the solution containing a combination of rat ANP and rabbit anti-ANP serum. The result indicated the presence of atrial natriuretic peptide (ANP) in the specific granules of myocytes of rat anterior venae cavae as well as in those of the atrium. The formation process of the specific granules and the possible way of the release of their contents were discussed.
10.Studies on the Preventive Action of Trichoderma spp. Against Rhizoctonia Damping-off of American Ginseng (Panax quinquefolium )
Chinese Traditional and Herbal Drugs 1994;0(02):-
Trichoderma spp.showed strong antagonistic action against Rhizoctonia solani in screening tests. In green house and field experiments, its effect in the prevention of rhizoctonia damping off of Panax quinquefolium was over 60%, which is superior than other conventional fungicides.