ObjectiveTo explore the ability of inhibition resistibility of multiple displacement amplification (MDA)in samples with inhibitors. To explain the application and value of MDA in forensic medicine by comparing with using magnetic beads methods(MBM)to purify sample.MethodsDifferent concentra-tions of hemoglobin and humid acid(HA)mixed with DNA samples and then divided the samples into MDA group, MBM group and control group.D3S1358locus was amplified and detected by polyacry-lamide gel electrophoresis detection system and AmpF?STR? IdentifilerTM Plus Kit-capillary electrophore-sis detection system.ResultsWhen hemoglobin concentrations exceed 1 ng/μL or HA concentrations ex-ceed 0.1 ng/μL, amplification products could not be obtained by single-locus system in control group. When hemoglobin concentration exceeds 100 ng/μL or HA concentrations exceed 1 ng/μL, the samples could not be amplified by MBM. Inhibitors in different concentrations were amplified successfully in MDA group without any influence from inhibitors.ConclusionMDA has the capability to remove the inhibi-tion of hemoglobin and HA, which is better than MBM and has a certain value in forensic practices.

Objective To observe the efficacy and side effect of olanzapine combined with tropisetron, dexamethasone for the pre-vention of highly emetogenic chemotherapy-induced nausea and vomiting ( CINV) . Methods A total of 78 patients with highly emetogen-ic single day chemotherapy were randomly divided into two groups:trial group ( olanzapine combined with tropisetron, dexamethasone n=40) and control group (Tropisetron Combined with Dexamethasone n=38). The control of acute CINV, delayed CINV and adverse reac-tions were observed. Results The control rates of acute vomiting in trial group and control group were 87. 5% vs 81. 6%, 65. 0% vs 57. 9% in acute nausea, 75. 0% vs 52. 6% in delayed vomiting, 32. 5% vs 13. 2% in delayed nausea. The trial group was better than the control group in delayed vomiting and delayed nausea which there was difference between them (P < 0. 05). There was no statistical differ-ence between the two groups in the incidence of adverse reactions (P>0. 05). Conclusion Olanzapine combined with tropisetron, dexa-methasone for the prevention was significantly better than tropisetron combined with dexamethasone in the control of delayed CINV in pa-tients received highly emetogenic chemotherapy.

Objective To analyze the relative factors of bone marrow suppression after chemotherapy with different-dose carboplatin and paclitaxel (TC) on the patients with ovarian cancer.Methods Sixty-two patients with ovarian cancer admitted in Beijing Obstetrics and Gynecology Hospital from January 2002 to December 2007, using TC regimen ,a total of 196 cycles of chemotherapy ,were divided into two groups by the doses of carboplatin [area under concentration-time curve(AUC) 4 -6 for low-dose,AUC >6 -7 for hight-dose, the carboplatin dose calculated with AUC] or by the doses of paclitaxel (135 -< 150 mg/m2 low-dose,150 - 175 mg/m2 hight-dose). After each TC cycle, the routine blood was test to determine the graduation of the marrow suppression, and then the correlation factors were analyzed with logistic regression. Results (1) The occurrence rate of bone marrow suppression:there were 159 cycles (81.1%) grade 0 - Ⅱ bone marrow suppression, while 37 cycles (18.9%) of grade Ⅲ - Ⅳ. (2) Factors related to bone marrow suppression:the results shown that there were not related to bone marrow suppression,which incluced cellular differentiation, tumor type, height, weight and paclitaxel dose(P>0.05). While,the different cycle, age, the later stages of tumor, serum creatinine concentration, endogenous creatinine clearance rate, AUC values were the relative factors of bone marrow suppression(P =0.000,0.000,0.018,0.033,0.001,0.000). Seven variables were conducted into the logistic regression and the results shown that the different cycles, the age, AUC grades were independent risk factors (P = 0.030,0.043,0.009).(3) When low-dose of paclitaxel was given, the occurrence of bone marrow suppression was related to the carboplatin dose AUC. The higher AUC values for carbopaltin were chosen, the higher of severe bone marrow suppression would happen. (4/14 vs 0,P=0.015). When the dose of high grade of paclitaxel was given, the occurrence of bone marrow suppression in cases with hight-dose carboplatin was statistically significant than that in cases treated with low-dose carboplatin [45.7%(16/35) vs 13.7% (17/124), P=0.000]. Conclusions The independent risk factors of myelosuppression after chemotherapy with TC regime on the patients with ovarian cancer including the cycles, age and AUC values. The carboplatin dose calculating with AUC is related to the occurrence of bone marrow suppression, the higher AUC values for carbopaltin would chosen,the higher of severe bone marrow suppression would be happen.

Bartter Syndrome ; complications ; Diarrhea ; etiology ; Female ; Humans ; Infant ; Male ; Syndrome

Female ; Humans ; Melanosis ; Middle Aged ; Occupational Diseases

Child ; China ; Humans ; Pediatrics ; Periodicals as Topic ; standards ; statistics & numerical data ; Publishing ; standards ; statistics & numerical data ; Quality Control

OBJECTIVE: To prepare and establish a release rate method for the determination of ganciclovir lung-targeted microcapsules.METHODS: The microcapsules were prepared by compound emulsion-dispersion solvent evaporation method.The in vitro drug release amount and release rate of the microcapsules were detected by UV spectrophotometry.RESULTS: The microcapsules were regular in shape with an average particle size of 13.87 ?m.The linear range of ganciclovir was 2～20 ?g?mL-1(r=0.999 9) and its average recovery was 99.96%(RSD=1.68%).The entrapment efficiency of the microcapsules was 63.85% and the drug release amount in vitro reached 40% in 24 hours.CONCLUSIONS: The microcapsules show significant sustained release character.

OBJECTIVE: To establish an HPLC method for the determination of the content of main components in racanisodamine hydrochloride(RH) injection.METHODS: The determination was performed on Phenomenex Luna C18 column with methanol-0.02% triethylamine(50∶50) used as mobile phase at a flow rate of 1.0 mL?min-1.The detecting wavelength was set at 220 nm;the column temperature was set at room temperature and the sample size was 20 ?L.RESULTS: The linear range of RH was 0.053～0.371 ?g?mL-1(r=0.999 9) and the average recover was 101.20%(RSD=0.23%,n=6).CONCLUSIONS: The method is proved to be simple,rapid,accurate and applicable for the determination of the content of RH in RH injection.

OBJECTIVE:To explore a new extraction method of baicalin. METHODS: A new continuous circular extraction equipment was adopted to extract baicalin. L9(43) orthogonal experiment was conducted to optimize the extraction process taking the content of total flavonoids as index with the number of period of batching cycle of solvent, extraction temperature and distillation time as factors. The new continuous circular extraction was compared with the aqueous extraction and acidic deposition method in terms of the yield of extractum, content of total flavonoids and baicalin content and the color of the extract. RESULTS: The best technical conditions for the new extraction method of baicalin were as follows: 2.5 periods in batching cycle of solvent, 50 ℃ in extraction temperature, and 6 h in distillation time. The new continuous circular extraction was significantly better than the aqueous extraction in terms of contents of total flavonoids and baicalin and the color of the extract. CONCLUSION: The new continuous circular extraction method is applicable for the extraction of baicalin.

OBJECTIVE:To analyze the adverse drug reactions(ADR) caused by anti-epileptic medicines used for patients in the Neurological Intensive Care Unit (ICU) for reference of rational use of anti-epileptic medicines. METHODS: The ADR induced by anti-epileptic drugs between May 2006 and Nov. 2007 in neurological ICU patients with secondary epilepsy were analyzed retrospectively. RESULTS: A total of 24 ADR cases (24/50,47.06%) induced by anti-epileptic drugs were reported from Neurological Intensive Care Unit(ICU). The ADR were mainly induced by carbamazepine,valproate sodium,oxcarbazepine and phenobarbital,and the ADR mainly appeared as skin rash,hypolekocytosis,hepatic dysfunction and hyponatremia etc,but all cured after prompt treatment. CONCLUSION: The risk/efficiency ratio should be considered in clinical use of anti-epileptic drugs,and those drugs showing remarkable efficacy and few adverse reactions should be used preferably to reduce the damage to patients caused by ADR.