The tissue engineering technology that developed in the 1980s brings a hope for the regeneration of human organs. It is feasible to construct by-pass vessels, which have no immune resistance but are capable of anticoagulation. At present, a huge number of researches have provided the necessary basis for the construction. However, there are still some difficulties on the points of induction and proliferation of endothelial cells, development of new materials for scaffold and co-culture of seed cells in scaffold. These difficulties have become the bottle neck of tissue engineered blood vessels. The tissue engineering vessels are nowadays absent in clinical practice. With the progress in researches of tissue engineering, it is strongly believed that human organs and tissues can be constructed using tissue engineering technology, thus satisfying the donors of organ transplantation.

AIM: To contrast the effect of small incision phacoemulsification and non-phacoemulsification to treat cataract.
METHODS: Totally 172 patients with cataract were divided into 2 groups ( n = 86 ) randomly. Patients in Group Phaco were treated with phacoemulsification and, while those in Group Siecs were treated with small incision extracapsular cataract surgery and intraocular lens implantation. They were all followed up for 1-3y. Functional examinations were made at 3d;1,6mo and last follow - up. The visual acuity, intraocular pressure, anterior chamber depth, average corneal power ( ACP ) , cylinder ( CYL ) , surface asymmetry index ( SAI ) and complications were contrasted between groups.
RESULTS: At 3d after operation, the visual acuity of Group Phaco was better than that of Group Siecs ( P<0-05), and CYL, SAI and intraocular pressure of Group Phaco were all significantly lower than those of Group Siecs (P<0. 05). At 1mo after operation, the visual acuity of Group Phaco was better than that of Group Siecs ( P<0-05), and CYL, SAI and intraocular pressure of Group Phaco were slightly lower than those of Group Siecs ( P<0. 05);there was no difference between the two group. At 6mo after operation, the visual acuity of Group Phaco was still better than that of Group Siecs (P<0. 05), and CYL, SAI and intraocular pressure of Group Phaco were flat as those of Group Siecs (P<0. 05); there was no difference between groups in those indexes above ( P > 0. 05 ). Furthermore, the APC and anterior chamber depth of each point- in - time had no significant difference between groups (P>0. 05). The incidences of complication were similar in two groups ( P> 0. 05 ); but with individual differences, patients of GradeII and Ⅲ in Group Phaco got lower complication rate (P<0. 05), while those of Grade Ⅳ higher (P<0. 05) than those of Group Siecs.
CONCLUSION: Our research shows that phacoemulsification has high effect in the treatment of patients with GradeII and Ⅲ nuclear hardness, while small incision extracapsular cataract surgery has high effect in the treatment of patients with Grade Ⅳ nuclear hardness.

The purpose of this study is to investigate the effect of Tetramethylpyrazin (TMP) on blood volume (△Er) and Hb oxygen sufficiency(F) of gastric mueosal su. perficial microcirculation in rat by reflectance spectrophotometry, on the formation of stress ulcer and on gastric mucosal blood flow (GMBF) by H_2 gas clearance. The results showed that TMP had no visible effect on △Er and F of normal anethetized rat. In hemorrhagic shock rat, F of gastric mucosa was increased from 13?10 to 22?5 (P

Asymmetric dimethylarginine(ADMA)is an endogenous inhibitor of nitric oxide synthase(NOS).it reduces nitric oxide production. ADMA is correlated with the risk factors of atherosclerosis, such as hypercholesterolemia, hypertension and hyperglycaemia. Accumulating evidence suggests that a derangement of the NOS pathway plays a critical role in atherogenesis and ADMA may participate in the process.

To investigate the correlation between cerebral infarction and carotid atherosclerosis.Methods:Bilateral carotid atherosclerotic plaques,intima-media thickness (IMT),and stiffness coefficient (β) in 60 patients with cerebral infarction were detected by high-frequency color Doppler imaging,and they were compared with the control group (n=60).Results:The detection rate of plaque in the cerebral infarction group was significantly higher than that in the control group (90% vs 28%,P<0.005); the constituent ratio of soft plaques in the cerebral infarction group was significantly higher than that in the control group (P <0.01),and the constituent ratio of flat and hard plaques was significantly lower than that in the control group (P<0.005); IMT and β values in the cerebral infarction group were significantly higher than those in the control group (P<0.01).Furthermore,IMT had significant positive correlation with β values (P<0.01).Conclusions:Carotid atherosclerosis is closely correlated with cerebral infarction.

Objective To evaluate the value of color Doppler flow imaging(CDFI) in the diagonosis of placental site trophoblastic tumor(PSTT). Methods After being examined by transvaginal CDFI, four of six patients underwent curettage and two of them had hysterectomy and attached by chemical treatment. All the patients were verified to have a PSTT by pathology. Results The image of PSTT in CDFI included different size at different site, low resistence index (RI) of vessel around the lesion, and absence of vessel in the lesion. Conclusions The characteristic image in CDFI is helpful to the diagnosis of PSTT.

Subclavian artery occlusion is a common peripheral artery occlusion disease of various causes,with incidence of 1.9％.The most common symptoms are dizziness,ataxia,and hemiplegia that are caused by subclavian steal syndrome.Surgery is the only therapeutic method for symptomatic patients.Open surgery remains an important role despite of the rapid progress in endovascular surgery.Main surgical method includes carotid-subclavian transposition (CST),carotid-subclavian bypass (CSB) and axillary-axillary bypass.Each one is suitable for different lesions and anatomies.Both the effectiveness and safety have been testified.

A protein was isolated by DEAE-cellulose Chromatography and ultracentrifuge from the fruits of Momordica charantid L., and identified by chemical and polyacrylamide gel electrophoresis methods. The molecular weight was 34 000; isoelectric point pH 8.4,N-end amino acid was leucine.It was composed of 17 kinds of amino acids. The protein has been shown to have antifertility action in male rats.

Breast cancer is one of reproduction-endocrine related tumors, so the risk factors including environmental and genetic factors which can disrupt anabolism and function of estradiol may increase the risk of breast cancer. Persistent environmental endocrine disruptor chemical (EDCs) can disrupt anabolism and function of endogenous hormone and the degree of disruption may depend on genetic polymorphism. EDCs and metabolic enzyme genetic polymorphism were discussed in this paper. To study the risk factors of breast cancer, both of the environmental factors and the genetic factors should be included.

Objective To establish culture method for Phyllanthus urinaria. Methods To study the possible effective factors of culture condition by comparing with different explants,sucrose,plant growth substance,and its ratio. Results The inductivity of stem was the highest about 55.56%,but callus of leaves could not be induced.6-BA was the most important factor on callus induction,followed by 2,4-D NAA and sucrose. Conclusion The optimal medium to induce and subculture is MS+2,4-D 0.5 mg/L+6-BA 1.0 mg/L+NAA 0.1 mg/L+sucrose 10 g/L.