One hundred mandibles of adult Chinese male unearthed in Shanghai without missing teeth, alveolar bone resorption or/and malposition of teeth were studied. Our data showed that the location of the mental foramen below the apex of the lower second premolar was the most common. Our results were compared with those of other investigators. The Clinical significance of iden.tifying the anatomical location of the mental foramen in dental practice is discussed

Objective To summarize analysis postpartum hemorrhage were treated by human recombinant coagulation factor Ⅶ, CARDS and other three amine butyl alcohol, psychological intervention treatment of clinical effects and the patient's quality of life.Methods Our hospital during July 2013 to December 2016, total 100 cases of postpartum hemorrhage patients treated with the control group of 50 patients with card other three amine butyl alcohol with psychological intervention therapy;observation group of 50 cases using human recombinant coagulation factor Ⅶ, CARDS and other three amine butyl alcohol, psychological intervention treatment, two groups of patients were reviewed treatment effect and postpartum quality of life.Results In the bleeding, bleeding time, postpartum anxiety score, depression score indicators, such as observation group is less than the control group, the difference to be markedly, with statistical significance (P<0.05);In the postpartum life quality score, the observation group is higher than the control group, the obvious differences, statistically significant (P<0.05);In terms of adverse reaction happening, observation group and control group difference is not obvious, not statistically significant .Conclusion Human recombinant coagulation factor Ⅶ, CARDS and other three amine butyl alcohol, psychological intervention treatment of postpartum hemorrhage, the clinical curative effect is distinct, can better improve the patients quality of life, improve patients' negative mood, is worthy of popularization and application.

Objective To study the effect of biofeedback electrical stimulation combined with pelvic floor muscle training in the postpartum rehabilitation treatment of pelvic floor function.Methods 320 puerperants who were rechecked at 42d after the delivery in our hospital from September 2011 to January 2013 were divided into two groups,patients in the observation group were treated with biofeedback electrical stimulation combined with pelvic floor muscle training ,patients in the control group were given without treatment intervention,then the pelvic floor muscle tension,vaginal muscle voltage and urinary incontinence rate of two groups at 3 month after the treatment were compared.Results The improvement of pelvic floor muscle tension and vaginal muscle voltage of observation group were significantly better than those of control group (<0.05),the urinary incontinence rate was significantly lower (<0.05) .Conclusion The biofeedback electrical stimulation combined with pelvic floor muscle training can effectively improve the pelvic floor muscle tension and decrease the urinary incontinence rate,so it can improve the life quality of puerperants.

Objective:To study the effect of adriamycin(ADR) as therapeutic agent inducing rabbit posterior capsular lens epithelial cell apoptosis and to assess the relationships between P53,Bcl-2 after lens extracapsular extraction.Methods:Twelve rabbit eyes were injected with 0.2mg ADR(0.4mg?L -1 ) during lens extracapsular extraction performed respectively in the third week and the eighth week after the operation.With TdT-mediated dUTP nick end labeling(TUNEL)and immunohistochemical(SABC) staining.The protein expression cell rate of P53 and Bcl-2 were detected.Results:Positive pericyte muclei labeled by TUNEL increased more significantly than those in the control guoup from the third week to the eighth week after the operation.P53 protein expression was significantly higher(P

Objective:To investigate the relationship among inflammatory reaction and cytokine levels,nitric oxide(NO) content in aqueous humor after alkali burn.Methods:12 adult New Zealand rabbits were divided into two groups(6 rabbits each):normal control group and alkali burn experimental group.Aqueous humor samples were aspirated on the alkali burn 1,3,7,14 and 30 days,and the total number of white blood cells in the aqueous humor and their subset distribution as well as tumor necrosis factor-? (TNF-?) and NO~-_2/NO~-_3 were counted.The data were analyzed using analysis of variance of SPSS software.Results:There was a higher number of inflammatory cells and a higher level of TNF-? and NO~-_2/NO~-_3 in alkali burn eyes than that in the normal control eyes.The number of mononuclear phagocyte and the levels of TNF-?,the content of NO~-_2/NO~-_3 in aqueous humor were the highest in alkali burn group in 7～14 days.The change of TNF-?,NO~-_2/NO~-_3 and inflammatory reaction were basically similar,that is ,the more serious the reaction,the higher the levels of the content.Conclusion:The intraocular inflammation after alkali burn is closely related to the change in cytokine levels and NO content in aqueous humor.

Objective To observe brain-protection effect of Jiannao Yizhi Capsule on ischemic stroke and explore its mechanism. Methods One hundred and eighty cases of ischemic stroke patients were divided into two groups. Sixty cases in control group were treated with conventional method, one hundred and twenty cases in treatment group were treated with Jiannao Yizhi Capsule on the basis of conventional method. Levels of endothelin (ET), calcitonin gene related-pepitide (CGRP), lipin peroxidation oxidase (LPO) and super oxidase dismutase (SOD) of the two groups were determined. Results There were significant differences in reducing levels of ET and LPO, enhancing levels of CGRP and SOD, improving neurological impairment, reducing levels of blood lipid and blood viscosity between the two groups (P

Objective:To establish mice model with learning and memory deficit induced by cerebral ischemia/reperfusion.Methods:The mice model induced by cerebral ischemia/reperfusion were made by bilateral occlusion of both common carotid arteries and ischemic hypotension/reperfusion.Locomotive activity,step-down test and water maze were used to reflect behavior changes,as well as cell morphologic changes were evaluated,respectively.And total anti-oxidant capacity and activities of SOD(superoxide dismutase)and MDA(malondialdehyde)were also measured.Results:The neuronal damage in model mice was significant,compared with sham group.And locomotive activity of model mice markedly decreased.The escape latency and time of electric shock of model mice significantly increased in step-down test;and 24 hours later the number of errors significantly increased,step-down latency and time of remaining on platform markedly decreased.In maze test,the spatial learning and memory ability of model mice significantly decreased with characteristics of increase in the number of errors and latency at D3,D4 and D5,and the total anti-oxidant capacity and activities of SOD were decreased and MDA level increased in model group compared with sham group,respectively.Conclusion:This model of mice cerebral ischemia/reperfusion is useful for studying learning and memory deficit.

AIM: To determine the content of ginsenoside Rg_1、Re and Rb_1 in Shenfu Injection (Radix et Rhizoma Ginseng Rubra,etc.) by HPLC-ELSD. METHODS: Separation was achieved on a Waters ODS column((4.6) mm?150 mm,5 ?m).Elution was carried out using a gradient of acetonitrile and water.Drift tube temperature:120 ℃,gas flow rate:1.8 mL/min. RESULTS: The calibration curves were linear in the range of 0.322-(3.22) ?g for ginsenoside Rg_1,0.290-2.90 ?g for ginsenoside Re,0.508-5.08 ?g for ginsenoside Rb_1. CONCLUSION: The method is simple,accurate,effective and feasible.It can be used to control the quality of Shenfu Injection.

Objective:To determine the expression of aquaporin 8 and its localization in human WISH cells and to explore the molecular and cellular mechanisms for water absorption across the amniotic membranes.Methods:Human amnion-derive WISH cells were cultured.Western analysis was used to quantify AQP8 expression level.Reverse transcripton-polymerase chain reaction(RT-PCR)was used to quantify AQP8 mRNA expression levels.Immunofluorescence was used to determine localization of AQP8 in WISH cells.Results:AQP8 mRNA protein was found in human WISH cells.By western-blot,the AQP8 proteim was detected at 34ku in human WISH cells.AQP8 labeling was observed in intracellular vesicular structures throughout the cytosol and plasma membrane.Conclusion:The study demonstrates the expression of aquaporin 8 in human WISH cells.These results suggest that aquaporin 8 may be a channel that mediates amniotic fluid resorption by way of intramembranous pathway.

We separated viable tumor-infiltrating lymphocytes (TIL) and reginal lymphaden lymphocytes (RNL) from 37 cases solid human tumors and their reginal lymphadens, by means of combing mechanical and enzymatic digestion and discontinuous density gradients. The total cell recovery were 0.42-78.4?10~6 per gm tumor and 8.8-194?10~6 per gm lymphaden. The purity TIL and RNL fraction were 61.1?15.4% and 82.5?8.2%, respectively. Activated TIL, PBL, RNL and splenic cell (SPC) by rIL-2 expanded 2.58?1.05?10~5 -fold, 2.7?2.6?10~3-fold, 8.25 ?2.85?10~6-fold and 5.64 ? 2.51?10~3-fold in an average of 39.2?11.2 days. Total cell numbers in RNLs and 26/37 (70.3%) of TILs were up to 10~(10).