1. Raspberry ketone attenuates high-fat diet-induced obesity by improving metabolic homeostasis in rats
Ali ALKALADI ; Mohammed BAESHEN ; Haytham ALI ; Mohamed AFIFI ; Ammar AL-FARGA ; Haytham ALI ; Aaser ABDELAZIM ; Mohamed AFIFI ; Aaser ABDELAZIM
Asian Pacific Journal of Tropical Biomedicine 2020;10(1):18-22
To investigate the molecular mechanisms of the antiobese effect of raspberry ketone against high-fat diet fed rats. Methods: Fifty adult male rats were randomly assigned to receive a standard diet, a high fat diet, and the high-fat diet and 0.5%, 1% or 2% raspberry ketone. Body weight, biochemical parameters and gene expression of CCAAT enhancer-binding protein (C/EBP)-d, fatty acid synthase (FAS), acetyl CoA carboxylase (ACC), peroxisome proliferator-activated receptor alpha (PPAR-a), hormone-sensitive lipase (HSL) and hepatic carnitine palmitoyltransferase 1 A (CPT1A) were investigated. Results: Body weight, blood glucose, insulin, total lipids, triacylglycerols, total cholesterol and low-density lipoprotein cholesterol were increased in high-fat diet fed rats. These high fat diet-induced changes were attenuated by treatment with raspberry ketone. High-density lipoprotein cholesterol was decreased in highfat diet fed rats but increased in rats treated with raspberry ketone. Molecular investigations showed induction of gene expression of C/EBP-d , FAS, ACC, CPT1A and inhibition of gene expression of PPAR-a and HSL in high-fat diet fed rats as compared with control. Raspberry ketone treament reversed these changes except CPT1A. Conclusions: Raspberry ketone can prevent obesity induced by a high-fat diet in rats by induction of the expression of enzymes, controlling lipolysis and fatty acids b oxidation as well as inhibition of gene expressions of adipogenic factors.
2. Allolobophora caliginosa coelomic fluid ameliorates gentamicin-induced hepatorenal toxicity in rats
Saad DAJEM ; Kareem MORSY ; Sara ALI ; Omar ABDELRADY ; Nouraldin SALAHALDIN ; Ahmed SOLIMAN ; Yasmin KAMAL ; Ammar ABDELAZIM ; Aya MOHAMED ; Kareem MORSY ; Ayman MOHAMED ; Sohair FAHMY
Asian Pacific Journal of Tropical Biomedicine 2020;10(9):411-416
Objective: To explore the efficacy of earthworm's coelomic fluid against gentamicin-induced hepatic and renal toxicity in rats. Methods: The animals were divided randomly into three groups (n = 6 per group): control, gentamicin, and Allolobophora caliginosa coelomic fluid-treated groups. Toxicity was established after injection of gentamicin daily for 8 days at a dose of 100 mg/kg. Aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total proteins, albumin, creatinine, urea, uric acid, malondialdehyde, glutathione, catalase and histopathology of tissues were investigated in the study. Results: Allolobophora caliginosa coelomic fluid significantly decreased urea, creatinine, uric acid, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and malondialdehyde levels while significantly increasing levels of total proteins, albumin, glutathione and catalase. The histopathological investigation showed partial restoration of renal and hepatic architecture. Conclusions: This study shows the potency of Allolobophora caliginosa coelomic fluid in improving the biochemical and histopathological changes induced by gentamicin in the liver and kidney of the rats.