In this report, an ameloblastoma case, which has been occurred on the left maxilla of a 10 year-old, male, German shepherd, was described on the clinical and morphological features. The mass with the dimensions of 5x2.5 cm was removed totally by maxillectomy and examined by radiography, magnetic resonance (MR) and biopsy. The tumor was histopathologically classified ameloblastoma of follicular type and in some fields showed acanthotic form. The invasiveness of the tumor was also observed to be high. This report is the first case of maxillary ameloblastoma in dogs in Turkey.
Ameloblastoma/*pathology/*veterinary ; Animals ; Dog Diseases/pathology ; Dogs ; Male ; Maxilla/pathology ; Maxillary Neoplasms/*pathology/*veterinary ; Neoplasm Invasiveness/pathology

Maxillofacial images must be examined to find pathologies not identified during clinical examination. Unicystic ameloblastoma (UA) extending to the mandibular body and ramus was neglected on initial panoramic radiographic examination. After orthodontic therapy, a huge lesion was observed clinically and through imaging exams. After the conservative surgery, no recurrence was observed during five years of follow-up. This case emphasized the need for careful evaluation of patient images focusing on the oral diagnosis before any dental treatment planning, including orthodontic therapy.
Ameloblastoma* ; Delayed Diagnosis* ; Dentistry ; Diagnosis, Oral ; Diagnostic Imaging ; Follow-Up Studies ; Humans ; Mandible* ; Pathology ; Recurrence

Adult ; Ameloblastoma ; pathology ; physiopathology ; Dental Implants, Single-Tooth ; adverse effects ; Female ; Humans ; Whole Body Imaging

OBJECTIVETo investigate the proliferative potential of the epithelial cells in odontogenic keratocyst, radicular cyst, dentigerous cyst and ameloblastoma.

METHODSDNA contents and ploidy of basal and spinous cells in keratocyst, radicular cyst, dentigerous cyst, and the peripheral column cells and central reticular cells in ameloblastoma were analysis respectively.

RESULTSThe more and higher DNA contents and the proliferating ploidy of keratocyst and ameloblastoma than those of radicular cyst and dentigerous cyst indicate the active proliferating potential. The spinous cells showed more active proliferating growth than the basal cells of keratocyst. The higher DNA contents of radicular cyst are related to the stimulus of the inflammation. The dentigerous cysts have more di-ploidy cells without active growth potential.

CONCLUSIONSThe active cell proliferating growth in keratocyst and ameloblastoma is probably the pathological basis of their local aggressive biological behavior.

Ameloblastoma ; genetics ; pathology ; Cell Division ; genetics ; DNA ; metabolism ; Humans ; Jaw Neoplasms ; genetics ; pathology ; Odontogenic Cysts ; genetics ; pathology ; Ploidies

Ameloblastoma ; pathology ; Bone Neoplasms ; classification ; genetics ; pathology ; Chondromatosis ; pathology ; Chondrosarcoma ; classification ; pathology ; Enchondromatosis ; pathology ; Humans ; Osteosarcoma ; classification ; pathology ; Sarcoma, Ewing ; pathology ; Societies, Medical ; United States ; World Health Organization

We report a case of adamantinoma of the tibia resembling fibrous dysplasia. The patient was a 55-year-old male, and complained of pain in the right lower leg. Roentgenographs showed a well demarcated osteolytic lesion with small foci of calcification and septation within the diaphysis of the distal tibia. The cortex was partially disrupted. Histologically, initial biopsy specimen showed fibrous connective tissue and trabeculae of immature woven bone, strongly suggestive of fibrous dysplasia. The lesion recurred and the second biopsy revealed nests of spindle cells and tubular epithelial structures embedded in granulation type-fibrous tissue. Immunohistochemically, both the nests of spindle cells and the tubular structures gave a positive reaction for cytokeratin. The present case emphasizes once again that histological diagnosis of fibrous dysplasia of the tibia should be made carefully with exclusion of the possibility of adamantinoma.
Ameloblastoma/*pathology/radiography ; Bone Neoplasms/*pathology/radiography ; Case Report ; Fibrous Dysplasia of Bone/*pathology/radiography ; Human ; Male ; Middle Age ; Tibia/*pathology/radiography

There have been many different histological typing of odontogenic tumours and numerous reports on the epidemiological studies of the odontogenic tumours depending upon various these histological typing. Neoplasms and other tumours related to the odontogenic apparatus was classified into 21 entities by WHO in 1992. The purpose of this study is to get the clinically basic data of the odontogenic tumours in Korean according to this newly made WHO histological classification. The total of 4913 biopsied specimens were reviewed from the dept of Oral Pathology, College of Dentistry, Yonsei University for the period of Jan. 1985. to Dec. 1996. and among them 156 cases were diagnosed into the odontogenic tumours. The following results were obtained: 1. Odontogenic tumours were 156 cases(3.18%) of the total 4913 biopsy cases. Only the 10 different benign entities were observed in 156 cases of odontogenic tumour. 2. The most frequent odontogenic tumour was ameloblastoma(42.3%) and followed odontomas (41.0%), adenomatoid odontogenic tumours (4.5 per cent) and ossifying fibroma(3.8%) in order. 3. Overall male to female ratio was 1.14(53.2%) to 1(46.8%), but female ratio is higher than male in odontoma(53.1%) and myxoma(75.0%) respectively. 4. 134 cases(85.9%) of all odontogenic tumours were observed under the age of forty. Age distribution showed 60 cases(38.5%) in the second decade, 37 cases(23.7%) in the third decade, 22 cases(14%) in the fourth decade and 15 cases(9.6%) in the first decade of life 5. The ratio of odontogenic tumours of the mandible to maxilla was about 2 : 1 . Odontogenic tumours occured predominantly in the molar region(29.6%) and gonial region(17.08%) of the mandible and anterior region(18.8%) of the maxilla. Ameloblastoma occurred mostly in the molar region(45.5%), gonial region(28.1%), ascending ramus region(10.7%) and premolar region(9.1%) of the mandible, while odontomas predominated in the anterior region(44.9%) of the maxilla. 6. Most ameloblastomas were related with more the impacted teeth(62.1%) and root resorption(53.0%) than teeth migration(27.3%). In case of odontoma, teeth impaction(62.5%), teeth migration(75.0%) and root resorption(7.8%) were observed. 7. In the ameloblastomas, facial swelling was the most frequent chief complaint(80.3%) and followed by pain(9.1%).
Age Distribution ; Ameloblastoma ; Bicuspid ; Biopsy ; Classification ; Dentistry ; Epidemiologic Studies* ; Female ; Humans ; Male ; Mandible ; Maxilla ; Molar ; Odontoma ; Pathology, Oral ; Tooth

OBJECTIVEThe expression of Ki-67 antigen of ameloblastoma was examined in order to investigate the different proliferation activity of histological variants of ameloblastoma and its clinical significance.

METHODS70 cases of different histological specimen of ameloblastoma were analyzed by immunohistochemical method using Ki-67 antibody. The Label Index was calculated in percentage of Ki-67 positive cells after examined with an image analysis system.

RESULTSThe results showed that the Labeled Index in malignant ameloblastoma was the highest 14.72% +/- 2.87%. The Labeled Index in solid ameloblastoma was in the middle, among which the follicular 4.42% +/- 1.05% was higher than the plexiform 3.64% +/- 1.23%. The Labeled Index in mono-cystic ameloblastoma was the lowest 2.21% +/- 1.09%.

CONCLUSIONThe results demonstrated that the proliferation activity varied according to the histological pattern of ameloblastoma. The prognosis with different proliferation activity was also varied accordingly.

Ameloblastoma ; metabolism ; pathology ; Humans ; Image Processing, Computer-Assisted ; Jaw Neoplasms ; metabolism ; pathology ; Ki-67 Antigen ; biosynthesis ; Neoplasm Recurrence, Local ; Odontogenic Tumors ; metabolism ; pathology

OBJECTIVETo clarify the clinicopathological spectrum and biologic behavior of unicystic ameloblastomas (UA).

METHODSRetrospective analysis of 33 UA was undertaken to relate clinicopathological variables to recurrence.

RESULTSThe clinical features of this series were in general agreement with that of the previous reports. UA tended to occur at an earlier age (mean 25.3 years), with a male predilection and predominant mandibular involvement (90.9%). Microscopically, the tumors demonstrated a generally monocystic growth pattern, with 8 being simple cystic, 10 comprising intraluminal nodules, and 15 exhibiting a conspicuous component of infiltrative tumor islands in the cyst capsule. Follow-up of 29 patients revealed 6 recurrences with an average interval of 7 years. Recurrence also related to histologic subtypes of UA, with those invading the fibrous wall having a rate of 35.7% but others 6.7%.

CONCLUSIONSDespite the fact that unicystic ameloblastoma may, in general, compare favorably with its solid or multicystic counterpart in terms of clinical behavior and response to treatment, the subsets of the maxillary lesions or tumors exhibiting mural invasion could have a high risk of recurrence.

Adolescent ; Adult ; Aged ; Ameloblastoma ; pathology ; Child ; Female ; Follow-Up Studies ; Humans ; Jaw Neoplasms ; pathology ; Male ; Middle Aged ; Neoplasm Recurrence, Local ; Odontogenic Cysts ; pathology

OBJECTIVETo study relation of the expression of hTERT mRNA and cyclin A, p53 protein, proliferation cell nuclear antigen (PCNA) in ameloblastoma (AB) and to investigate clinical biological characteristics of AB.

METHODSThe hTERT mRNA was detected by in situ hybridization, cyclin A, p53 protein and PCNA by SP method. Normal oral mucosa and odontogenic keratocyst (OKC) are comparition.

RESULTSThe positive ratio of hTERT mRNA was 1/7 cases in normal oral mucosa. The expression of cyclin A, p53 and PCNA in normal oral mucosa were similar, and the positive cells distributed in stratum basale. In OKC, the positive cells distributed in stratum basale and super-strrtum basale. And the positive ratio of hTERT, cyclin A, p53, PCNA in OKC was 14/16 cases, 4/32 cases, 11/25 cases, 5/9 cases, respectively. In AB, the positive ratio of hTERT mRNA, cyclin A, p53 protein and PCNA was 94.4% (51/54), 66.7% (40/60), 85.7% (42/49) and 78.1% (25/32), respectively. A strong correlation was found between hTERT mRNA with cyclin A, p53 protein and PCNA (r(s) = 0.914, 0.848, 0.804, P < 0.05).

CONCLUSIONSThe expression of hTETR mRNA, cyclin A, p53 and PCNA is different in different tissues and lesions, and correlates with cell differentiation and clinical biology behaviour. Telomerase activity is related to cumulation of p53 protein, related to cell proliferation and differentiation, regulated by cyclin A, and higher in S phase.

Ameloblastoma ; metabolism ; pathology ; Cyclin A ; biosynthesis ; Humans ; Jaw Neoplasms ; metabolism ; pathology ; Proliferating Cell Nuclear Antigen ; biosynthesis ; RNA, Messenger ; biosynthesis ; Telomerase ; biosynthesis ; genetics ; Tumor Suppressor Protein p53 ; biosynthesis