1.Antioxidant effects of D-004, a lipid extract from the Roystonea regia fruit, on the plasma of healthy men.
Ernesto LÓPEZ ; Vivian MOLINA ; José ILLNAIT ; Ambar OYARZÁBAL ; Lilia C FERNÁNDEZ ; Rosa MÁS ; Rafael GÁMEZ ; Julio C FERNÁNDEZ ; Sonia JIMÉNEZ ; Meilis MESA ; Ivón HOLLANDS ; Sarahí MENDOZA
Asian Journal of Andrology 2009;11(3):385-392
The aim of this study was to conduct a randomized, double-blind and placebo-controlled study to investigate the effects of D-004, a lipid extract of the Roystonea regia fruit that prevents testosterone- and phenylepinephrine-induced prostate hyperplasia in rodents, on plasma oxidative markers in healthy men. We enrolled male volunteers (20-55 years) in good health and without lower urinary tract symptoms. Thirty-four eligible participants were randomized to placebo or D-004 (320 mg) capsules administered daily for 6 weeks. An interim check-up and a final visit were conducted after 3 and 6 weeks of therapy, respectively. Physical examinations were performed at each visit, and laboratory tests were performed at baseline and at treatment completion. Oxidative variables included plasma malondialdehyde (MDA), total hydroxyperoxides (TOH), sulphydryl (SH) groups and total antioxidant status (TAS). We assessed treatment compliance and addressed adverse experiences (AEs) at weeks 3 and 6. At week 6, with D-004, the mean reductions of plasma MDA (26.7%), TOH (18.8%) and SH groups (31.6%), and the mean increase of TAS (35.3%) were significantly different from those of placebo (P<0.001 for plasma TAS, P<0.0001 for all other comparisons). D-004 did not differ from the placebo in safety indicators. There were two withdrawals (both in the D-004 group), with one due to dyspepsia (the only AE during the trial). In conclusion, D-004 displayed antioxidant effects on plasma oxidative markers in healthy men, which was consistent with findings from laboratory experimental studies.
Adult
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Antioxidants
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administration & dosage
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adverse effects
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Arecaceae
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Biomarkers
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blood
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Humans
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Lipid Peroxidation
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drug effects
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Lipids
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Male
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Middle Aged
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Oxidative Stress
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drug effects
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Placebos
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Plant Extracts
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administration & dosage
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adverse effects
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Prostatic Hyperplasia
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drug therapy
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metabolism
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Young Adult
2.Protective effects of D-005, a lipid extract from Acrocomia crispa fruits, against ischemia/reperfusion-induced acute kidney injury in rats
Ambar OYARZÁBAL-YERA ; Sandra RODRÍGUEZ-SALGUEIRO ; Nelson MERINO-GARCÍA ; Leyanis OCAÑA-NÁPOLES ; Lucía GONZÁLEZ-NÚÑEZ ; Licet MENA-VALDÉS ; Zullyt ZAMORA-RODRÍGUEZ ; José A MEDINA-PÍREZ ; Sonia JIMÉNEZ-DESPAIGNE ; Vivian MOLINA-CUEVAS
Kidney Research and Clinical Practice 2019;38(4):462-471
BACKGROUND: Acute kidney injury (AKI) induced by renal ischemia/reperfusion (IR) is associated with enhanced production of reactive oxygen species in renal tissues. D-005, a lipid extract obtained from Acrocomia crispa fruit, has previously shown antioxidant effects. The aim of this work was to evaluate the effects of D-005 on renal IR-induced AKI in rats.METHODS: Rats were randomized into seven groups including a negative control group (vehicle) without AKI and six groups with renal IR-induced AKI as follows: a positive control (vehicle); D-005 treatment at 25, 100, 200, or 400 mg/kg; and dexamethasone at 3 mg/kg. All treatments were orally administered as single doses 1 hour before AKI induction. Biomarkers (serum creatinine, urea, and uric acid concentrations), oxidative variables, and histopathological AKI changes were evaluated in blood and kidney tissues.RESULTS: All D-005 doses protected against IR-induced AKI in rats by significantly decreasing biomarkers and histopathological AKI changes as assessed by reduced serum concentrations of creatinine, urea, and uric acid. In addition, all D-005 doses decreased tubular damage, as shown by fewer detached cells and casts in the tubular lumen. D-005 reversed oxidation disturbance markers by decreasing malondialdehyde and sulfhydryl group concentrations in plasma and in kidney homogenates and by increasing kidney catalase activity. Dexamethasone, the reference substance, protected against IR-induced AKI in rats by reducing biochemical and histological variables of renal damage in a similar manner.CONCLUSION: Administration of single oral doses of D-005 markedly and significantly protected against renal IRinduced AKI, possibly due to its known antioxidant effects.
Acute Kidney Injury
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Animals
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Antioxidants
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Biomarkers
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Catalase
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Creatinine
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Dexamethasone
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Fruit
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Kidney
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Malondialdehyde
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Plasma
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Rats
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Reactive Oxygen Species
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Urea
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Uric Acid