INTRODUCTION: Concerns have been increased about the use of mobile phones in hospitals as they may be vehicles for the transmission of hospital-acquired infections. This study aimed to compare the effectiveness of 70% isopropyl alcohol wipes with bleach-based wipes in decreasing bacterial colony counts of mobile phones of staff nurses. METHODS: Mobile phones of staff nurses in the UERM Hospital were assigned to be disinfected with 70% isopropyl alcohol wipes or bleach-based wipes. Mobile phones were swabbed using standard techniques before and after disinfection with 70% isopropyl alcohol wipes or bleach-based wipes. Post-disinfection colony counts were compared with baseline counts in each group and compared between the two test groups. RESULTS: There was a significant decrease in the post-disinfection mean colony count compared with the mean baseline colony count in both the 70% isopropyl alcohol wipes (p < 0.001) and bleach-based wipes (p = 0.002) groups. The decrease in the 70% isopropyl alcohol wipes group was bigger (121,635 vs 85,769 CFU/mL). The mean post-disinfection colony count of the 70% isopropyl alcohol wipes was significantly lower (p = 0.007) than the other group. CONCLUSION Both 70% isopropyl alcohol wipes and bleach-based wipes are effective in decreasing bacterial colony counts of mobile phones of staff nurses. The alcohol wipes resulted in a greater decrease in colony count compared with the bleach wipes.