1. Tioxolone niosomes exert antileishmanial effects on Leishmania tropica by promoting promastigote apoptosis and immunomodulation
Maryam HAKIMI PARIZI ; Iraj SHARIFI ; Ali KEYHANI ; Mahshid MOSTAFAVI ; Ahmad KHOSRAVI ; Daryoush GHAFFARI ; Saeedeh FARAJZADEH ; Abbas PARDAKHTY ; Mohammad PARIZI ; Hamid SHARIFI ; Mehdi BAMOROVAT
Asian Pacific Journal of Tropical Medicine 2019;12(8):365-374
Objective: To explore the antileishmanial effect of tioxolone and its niosomal form against Leishmania tropica. Methods: Tioxolone niosomes were prepared by the hydration method and were evaluated for morphology, size, release study, and encapsulation efficiency. The cytotoxicity of tioxolone and its niosomal form was measured by MTT assay, leishmanicidal activity against promastigote and amastigote by MTT assay, apoptosis by flow cytometry, IL-12, IL-10 and metacaspase gene expression levels by q-PCR. Results: Span/Tween 40 and Span/Tween 60 niosomes had good physical stability as depicted in their size distribution curves and high encapsulation efficiency (>99%). The release profile of the entrapped compounds showed Fickian's model of tioxolone delivery based on diffusion through lipid bilayers. With the IC
Result Analysis
Print
Save
E-mail