1.Glutamate dehydrogenase antigen detection in Plasmodium falciparum infections.
Neira de DOMINIGUEZ ; Alexis RODRIGUEZ-ACOSTA
The Korean Journal of Parasitology 1996;34(4):239-246
The usefulness of malaria diagnosis by Plasmodium falciparum-GDH (NADP+), obtained by affinity chromatography, is demonstrated in ELISA assays, testing IgG antibodies against GDH (NADP+) from patients with acute malaria, who have had two or more episodes of malaria, or from sera of hyperimmune patients. GDH (NADP+) thermal stability was demonstrated in a high heat resistance assay. The immunofluorescence assay demonstrated that anti-culture (P. falciparum) supernatant serum and anti-GDH (NADP+) of Proteus spp, recognized epitopes in Venezuelan isolates, and Colombian and Brasilian malarial strains. The antigen is soluble, with high specificity, is a potent immunogen and is thermoresistant.
parasitology-protozoa
;
antigen
;
enzymes
;
glutamate dehydrogenase
;
malaria
;
diagnosis
;
Plasmodium berghei
;
Plasmodium cathemerium
;
Plasmodium falciparum
;
Plasmodium vivax
;
enzyme-linked immunosorbent assay