1.Evidence for obtaining a second successive semen sample for intrauterine insemination in selected patients: results from 32 consecutive cases.
Alejandra ORTIZ ; Rita ORTIZ ; Evelyn SOTO ; Jonathan HARTMANN ; Alejandro MANZUR ; Marcelo MARCONI
Clinical and Experimental Reproductive Medicine 2016;43(2):102-105
OBJECTIVE: The goal of this study was to compare the semen parameters of two successive samples obtained within an interval of less than 60 minutes from patients planning to undergo intrauterine insemination (IUI) whose first samples exhibited low semen quality. METHODS: Thirty-two consecutive patients were enrolled in the study. On the day of IUI, the semen analysis of the samples initially presented by all patients met at least two of the following criteria: sperm concentration <5×10(6)/mL, total sperm count <10×10(6), progressive sperm motility (a+b) in the native sample <30%, and total motile sperm count (TMSC) <4×10(6). A successive semen sample was obtained no more than 60 minutes after the first sample. RESULTS: Compared to the first sample, the second exhibited significantly (p<0.05) improved sperm concentration, TMSC, progressive motility, and vitality. Regarding TMSC, the most critical parameter on the day of IUI, 23 patients (71.8%) improved it, while nine (28.2%) displayed poorer outcomes. CONCLUSION: In defined cases, requesting a second successive ejaculate on the day of insemination may result in a high percentage of cases in an improvement of the quality of the sample.
Humans
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Insemination*
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Semen Analysis
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Semen*
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Sperm Count
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Sperm Motility
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Spermatozoa
2.Restoration and conservation of anatomic pieces
Camila CÁRDENAS GUERRERO GUZMÁN ; Karen Alejandra PÉREZ DÍAZ ; María Paula RUÍZ DÍAZ ; Valentina Díaz SÁNCHEZ ; Andrés Camilo ARIZA AGUIRRE ; Laura Catalina CANTOR ALFONSO ; Camila Andrea SUÁREZ ORTIZ ; Davide Faliero GONZÁLEZ ÁLVAREZ ; Laura Mariana SIERRA BURGOS ; Yobany QUIJANO BLANCO
Anatomy & Cell Biology 2019;52(3):255-261
In this study, a restoration process was developed with potassium hydroxide (KOH), in order to improve each of the structures for their posterior fixation, through the use of new methods such as the Chilean conservative fixative solution (SFCCh), with exceptional results. Restore anatomical pieces corresponding to corpse and organs, being these last set with the SFCCh. In this work dealt with processes of restoration with potassium hydroxide, sodium chloride, and sodium hypochlorite, the process began with the cleanliness and suture of the structures for subsequent fixing in Chilean conservative fixative solution, making use of a corpse and different anatomical parts. Work based on items found in the database, Elsevier, Science Direct, ProQuest, and MEDLINE. At the end of the process of restoration and conservation of the anatomical pieces, was observed an improvement in muscle pigment with decrease of rigidity in the specimen, additionally a recovery of appearance in the vascular-nervous elements was achieved. The organs were much more malleable and the structures facilitate the identification of specific details, its subsequent immersion in SFCCh allows the longer preservation of the obtained results. The restoration with potassium hydroxide allows the improvement in the appearance of the different anatomical structures and simultaneously to facilitate its study. The SFCCh is an alternative that replaces partially the use of formaldehyde. In addition, it presents toxicity reduction.
Cadaver
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Formaldehyde
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Immersion
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Potassium
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Sodium Hydroxide
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Sodium Hypochlorite
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Sutures