1.Renin-Angiotensin-Aldosterone System (RAAS) Gene Polymorphism as a Risk Factor of Coronary In-Stent Restenosis.
Sung Kee RYU ; Eun Young CHO ; Hyun Young PARK ; Eun Kyoung IM ; Yangsoo JANG ; Gil Ja SHIN ; Won Heum SHIM ; Seung Yun CHO
Yonsei Medical Journal 2002;43(4):461-472
Intimal proliferation is a main cause of in-stent restenosis. Over-excretion of angiotensin I converting enzyme (ACE) and aldosterone is reported to stimulate intimal hyperplasia and the genetic effect of these molecules may alter the process of in-stent restenosis. We hypothesized that the genetic polymorphisms that alter the expression of genes such as ACE I/D, CYP11B2-344C/T, and AGT M235T can affect in-stent restenosis. We analyzed the angiographic and clinical data of 238 patients (272 stents) who underwent coronary stenting and follow-up angiography, and analyzed the genotypes of ACE I/D, CYP11B2-344T/C, and AGT M235T. There was no significant difference in age, sex, or lipid profiles between the patent and restenosis groups. Diabetes mellitus was more frequent in the binary restenosis group. Quantitative computer-assisted angiographic (QCA) analysis revealed that the risk of in-stent restenosis increased with lesion length and was inversely proportional to post- stenting minimal luminal diameter (MLD) and reference diameter. There was no difference in the frequency of binary restenosis between genotypes in each of the three genes. However, follow-up MLD was significantly smaller in the ACE DD genotype than in the ACE II or ID genotypes. Defining restenosis as MLD 2 mm, the restenosis rate was significantly higher in the ACE DD genotype than in the ACE II or ID genotypes. There was no significant synergistic effect between the three gene polymorphisms. In conclusion, while the ACE I/D polymor phism promoted the progress of in-stent restenosis and was of clinical significance, the other potential variables examined did not correlate with in-stent restenosis.
Adult
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Aged
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Aldosterone Synthase/*genetics
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Angiotensinogen/*genetics
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Coronary Restenosis/*etiology/genetics
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Female
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Genotype
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Human
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Logistic Models
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Male
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Middle Age
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Peptidyl-Dipeptidase A/*genetics
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*Polymorphism (Genetics)
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Risk Factors
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*Stents
2.Genetic Analyses of the Chimeric CYP11B1/CYP11B2 Gene in a Korean Family with Glucocorticoid-Remediable Aldosteronism.
Ihn Suk LEE ; Seul Young KIM ; Hye Won JANG ; Min Kyeong KIM ; Ju Hee LEE ; Yun Hyeong LEE ; Young Suk JO
Journal of Korean Medical Science 2010;25(9):1379-1383
Glucocorticoid-remediable aldosteronism (GRA) is an autosomal-dominant inheritable form of hyperaldosteronism with early onset hypertension. GRA is caused by unequal crossing-over of the steroid 11beta-hydroxylase (CYP11B1) and aldosterone synthase (CYP11B2) genes. As a result of chimeric gene duplication, aldosterone is ectopically synthesized in the adrenal zona fasciculata under the control of adrenocorticotropin. Here, we describe three cases of GRA in a Korean family. The proband-a 21-yr-old female-was incidentally found to have high blood pressure (170/108 mmHg). Her 46-yr-old father had been treated twice for cerebral hemorrhage at the ages of 29 and 39 yr. Her 15-yr-old brother had a 2-yr history of hypertension; however, he was never treated. Their laboratory test results showed normokalemia, hyporeninemia, hyperaldosteronism, and a high plasma aldosterone concentration-to-plasma renin activity ratio. Normal saline loading failed to suppress aldosterone secretion. However, dexamethasone administration effectively suppressed their plasma aldosterone concentrations. Following genetic analyses with PCR and direct sequencing to document the chimeric gene and crossover site, respectively, we identified CYP11B1/CYP11B2 and determined the breakpoint of unequal crossover to be located between intron 2 of CYP11B1 and exon 3 of CYP11B2.
Adolescent
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Aldosterone/blood
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Aldosterone Synthase/*genetics
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Asian Continental Ancestry Group/*genetics
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Dexamethasone/therapeutic use
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Family
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Female
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Glucocorticoids/*therapeutic use
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Humans
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Hyperaldosteronism/diagnosis/drug therapy/*genetics
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Hypertension/etiology
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Magnetic Resonance Angiography
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Male
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Middle Aged
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Renin/blood/metabolism
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Republic of Korea
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Sequence Analysis, DNA
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Steroid 11-beta-Hydroxylase/*genetics
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Young Adult