1.¹²³I-Labeled oxLDL Is Widely Distributed Throughout the Whole Body in Mice
Atushi NAKANO ; Hidekazu KAWASHIMA ; Yoshinori MIYAKE ; Tsutomu ZENIYA ; Akihide YAMAMOTO ; Kazuhiro KOSHINO ; Takashi TEMMA ; Tetsuya FUKUDA ; Yoshiko FUJITA ; Akemi KAKINO ; Shigehiko KANAYA ; Tatsuya SAWAMURA ; Hidehiro IIDA
Nuclear Medicine and Molecular Imaging 2018;52(2):144-153
PURPOSE: Oxidized low-density lipoprotein (oxLDL) plays a key role in endothelial dysfunction, vascular inflammation, and atherogenesis. The aim of this study was to assess blood clearance and in vivo kinetics of radiolabeled oxLDL in mice.METHODS: We synthesized ¹²³I-oxLDL by the iodine monochloride method, and performed an uptake study in CHO cells transfected with lectin-like oxLDL receptor-1 (LOX-1). In addition, we evaluated the consistency between the ¹²³I-oxLDL autoradiogram and the fluorescence image of DiI-oxLDL after intravenous injection for both spleen and liver. Whole-body dynamic planar images were acquired 10 min post injection of ¹²³I-oxLDL to generate regional time-activity curves (TACs) of the liver, heart, lungs, kidney, head, and abdomen. Regional radioactivity for those excised tissues as well as the bladder, stomach, gut, and thyroid were assessed using a gamma counter, yielding percent injected dose (%ID) and dose uptake ratio (DUR). The presence of ¹²³I-oxLDL in serum was assessed by radio-HPLC.RESULTS: The cellular uptakes of ¹²³I-oxLDL were identical to those of DiI-oxLDL, and autoradiograms and fluorescence images also exhibited consistent distributions. TACs after injection of ¹²³I-oxLDL demonstrated extremely fast kinetics. The radioactivity uptake at 10 min postinjection was highest in the liver (40.8 ± 2.4% ID). Notably, radioactivity uptake was equivalent throughout the rest of the body (39.4 ± 2.7% ID). HPLC analysis revealed no remaining ¹²³I-oxLDL or its metabolites in the blood.CONCLUSION: ¹²³I-OxLDL was widely distributed not only in the liver, but also throughout the whole body, providing insight into the pathophysiological effects of oxLDL.
Abdomen
;
Animals
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Atherosclerosis
;
CHO Cells
;
Chromatography, High Pressure Liquid
;
Cricetinae
;
Fluorescence
;
Head Kidney
;
Heart
;
Inflammation
;
Injections, Intravenous
;
Iodine
;
Kinetics
;
Lipoproteins
;
Liver
;
Lung
;
Methods
;
Mice
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Radioactivity
;
Spleen
;
Stomach
;
Thyroid Gland
;
Urinary Bladder
2.Non-invasive continuous blood pressure monitoring using the ClearSight system for pregnant women at high risks of post-partum hemorrhage: comparison with invasive blood pressure monitoring during cesarean section
Takuya MISUGI ; Takashi JURI ; Koichi SUEHIRO ; Kohei KITADA ; Yasushi KURIHARA ; Mie TAHARA ; Akihiro HAMURO ; Akemi NAKANO ; Masayasu KOYAMA ; Takasi MORI ; Daisuke TACHIBANA
Obstetrics & Gynecology Science 2022;65(4):325-334
Objective:
This study aimed to investigate the accuracy and precision of continuous, non-invasive blood pressure obtained using the ClearSight system by comparing it with invasive arterial blood pressure, and to assess the hemodynamic changes using invasive methods and the ClearSight system in patients undergoing cesarean section.
Methods:
Arterial pressure was measured invasively with an intra-arterial catheter and non-invasively using the ClearSight system during cesarean section in patients with placenta previa or placenta accreta. Blood pressure measurements obtained using these two means were then compared.
Results:
Total 1,277 blood pressure measurement pairs were collected from 21 patients. Under Bland-Altman analysis, the ClearSight system demonstrated an acceptable accuracy with a bias and standard deviation of 8.8±13.4 mmHg for systolic blood pressure, -6.3±7.1 mmHg for diastolic blood pressure, and -2.7±8.0 mmHg for median blood pressure. Cardiac index levels were significantly elevated during fetal delivery and 5 minutes after placental removal, and systemic vascular resistance index levels were significantly decreased during fetal delivery and 40 minutes after placental removal.
Conclusion
In patients undergoing cesarean section, the ClearSight system showed excellent accuracy and precision compared to that of the currently used invasive monitoring system.
3.¹²³I-Labeled oxLDL Is Widely Distributed Throughout the Whole Body in Mice
Atushi NAKANO ; Hidekazu KAWASHIMA ; Yoshinori MIYAKE ; Tsutomu ZENIYA ; Akihide YAMAMOTO ; Kazuhiro KOSHINO ; Takashi TEMMA ; Tetsuya FUKUDA ; Yoshiko FUJITA ; Akemi KAKINO ; Shigehiko KANAYA ; Tatsuya SAWAMURA ; Hidehiro IIDA
Nuclear Medicine and Molecular Imaging 2018;52(2):144-153
PURPOSE:
Oxidized low-density lipoprotein (oxLDL) plays a key role in endothelial dysfunction, vascular inflammation, and atherogenesis. The aim of this study was to assess blood clearance and in vivo kinetics of radiolabeled oxLDL in mice.
METHODS:
We synthesized ¹²³I-oxLDL by the iodine monochloride method, and performed an uptake study in CHO cells transfected with lectin-like oxLDL receptor-1 (LOX-1). In addition, we evaluated the consistency between the ¹²³I-oxLDL autoradiogram and the fluorescence image of DiI-oxLDL after intravenous injection for both spleen and liver. Whole-body dynamic planar images were acquired 10 min post injection of ¹²³I-oxLDL to generate regional time-activity curves (TACs) of the liver, heart, lungs, kidney, head, and abdomen. Regional radioactivity for those excised tissues as well as the bladder, stomach, gut, and thyroid were assessed using a gamma counter, yielding percent injected dose (%ID) and dose uptake ratio (DUR). The presence of ¹²³I-oxLDL in serum was assessed by radio-HPLC.
RESULTS:
The cellular uptakes of ¹²³I-oxLDL were identical to those of DiI-oxLDL, and autoradiograms and fluorescence images also exhibited consistent distributions. TACs after injection of ¹²³I-oxLDL demonstrated extremely fast kinetics. The radioactivity uptake at 10 min postinjection was highest in the liver (40.8 ± 2.4% ID). Notably, radioactivity uptake was equivalent throughout the rest of the body (39.4 ± 2.7% ID). HPLC analysis revealed no remaining ¹²³I-oxLDL or its metabolites in the blood.
CONCLUSION
¹²³I-OxLDL was widely distributed not only in the liver, but also throughout the whole body, providing insight into the pathophysiological effects of oxLDL.