Objective To observe the efficacy and safety of domestic ziprasidone injection in the treatment of excitement and agitation in acute period of schizophrenia.Methods 60 patients with schizophrenia excited were randomly divided into two groups,30 cases in each group.The treatment group was given ziprasidone 20 to 40mg/day by intramuscular injection,the control group was given haloperidol 10-20mg/day by intramuscular injection,the two groups were observed for three days.The positive and negative symptoms scale(PANSS)in the excitation factor(EC) points rate was used to assess efficacy,symptoms scale(TESS)was used to evaluate the adverse reaction.Before treatment,2,6,24,48,72 hours after treatment,the positive and negative symptoms scale of excitement factor (PANSS-EC)and symptoms scale(TESS)were evaluated.Results 72 hours after treatment,the PANSS -EC score in the ziprasidone group was obviously lower,the points rate was (69 ±18)%,the total effective rate was 83.3%,which of the haloperidol group were (71 ±26)%,86.7%,the differences between the two groups were not statistically significant (P>0.05).The incidence of adverse reactions between the ziprasidone group and haloperidol group had no statistically significant difference(P>0.05).But in terms of the incidence of vertebral body system,the ziprasidone group(4 cases)was obviously less than the haloperidol group(13 cases),the difference was statistically significant(χ2 =6.65,P<0.05).Conclusion Ziprasidone injection is effective in the treatment of agitation in acute phase of schizophrenia,with less side effect,higher safety.

In the present study, packaging system composed of pAAV-CMV-GFP, pAAV-RC and pHelper were transfected into human embryonic kidney 293 cells (HEK293 cells) mediated by polyethyleneimine (PEI) to explore an optimal transfection condition. Different total plasmid DNA dosages (1, 2, 3, 4, 5, 6 μg) and different PEI/Plasmid ratios (1:1, 3:1, 5:1, 7:1) were tested with detection of green fluorescence protein (GFP) with ImagePro Plus6. 0 Software. Then transfection efficiency of the optimized transfection system was further observed for different time periods(12, 24, 36, 48, 60, 72 h). The results showed that total plasmid dosage of 4 μg/well with PEI/plasmid ratio of 3 : 1-5 : 1 was an efficient transfection condition. Transfection efficiency-time curve was an S-shaped curve. Transfection efficiency reached a plateau at 60 h after transfection. The optimized conditions for PEI-mediated transfection at the optimal time result in enhanced transfection efficiency of triple plasmid into HEK293 cells.
Green Fluorescent Proteins ; HEK293 Cells ; Humans ; Plasmids ; Polyethyleneimine ; Transfection ; methods

OBJECTIVE: To evaluate the effect of video-assisted thoracoscopic sympathectomy at the T4 level on plantar hyperhidrosis in the treatment of palmoplantar hyperhidrosis.
 METHODS: The clinical data of 28 patients with primary palmoplantar hyperhidrosis, who were admited in our hospital from June 2009 to May 2014, was analyzed. All patients were qualified to bilateral thoracoscopic transaction of the sympathetic chain at the thoracic level T4. Patients completed a self-administered hyperhidrosis questionnaire and scoring before and after procedure. Follow-up data were obtained at 1 and 6 months after the surgery.
 RESULTS: Endoscopic thoracic sympathectomy at the thoracic level T4 was performed successfully for all cases. Palmar hyperhidrosis was completely alleviated after the operation and no recurrence was observed during follow-up. The ratio for initial improvement of plantar hyperhidrosis was 28.6% (8/28) at 1 month after the surgery followed by a recurrence of plantar hyperhidrosis. No case continued to show the improvement of palmoplantar hyperhidrosis at 6 months after the sympathectomy. Twenty-seven patients (96.4%) were very satisfied with the outcome of the operation, 1 patient (3.6%) satisfied and no patient regretted the surgical procedure.
 CONCLUSION T4 thoracoscopic sympathectomy could initialliy alleviate plantar hyperhidrosis in some patients with palmoplantar hyperhidrosis, but the improvement was not sustained over a long period. It could not be used to treat plantar hyperhidrosis.
Foot ; Humans ; Hyperhidrosis ; Recurrence ; Surveys and Questionnaires ; Sympathectomy ; Sympathetic Nervous System ; Thoracic Surgery, Video-Assisted

OBJECTIVE: To assess the quality of life in patients with primary palmar hyperhidrosis before and after T4 endoscopic thoracic sympathicotomy (ETS) using the modified rating scales system for life quality.
 METHODS: Between June, 2009 and May, 2014, forty-eight patients with primary palmar hyperhidrosis received ETS at the thoracic level T4. Patients completed self assessment of life quality by the modified rating scales system before and after the surgery. Follow-up data including effectiveness of operation, patient satisfaction and life quality were obtained at 1 and 6 months after the surgery.
 RESULTS: ETS at the thoracic level T4 was performed successfully for all cases. No mortality or serious complications were observed. No one needed thoracotomy in the period of surgery. Mild or moderate compensatory sweating was appeared in 38 cases (79.1%) or 1 case (2.1%), respectively. None severe case was observed after T4 ETS. About 97.9% of the patients were very satisfied with the result of the operation and no patient regretted the surgical procedure. All patients answered the quality of life (QoL) questionnaire and showed the improvement of QoL after the procedure (F=763.67, P<0.001).
 CONCLUSION Primary palmar hyperhidrosis led to the reduction of life quality. ETS at T4 level could reduce sweating production and improve QoL in patients with primary palmar hyperhidrosis.
Endoscopy ; Humans ; Hyperhidrosis ; surgery ; Patient Satisfaction ; Quality of Life ; Surveys and Questionnaires ; Sweating ; Sympathectomy ; Thoracotomy ; Treatment Outcome