1.Construction of telemedicine information system based on military integrated information network
Zhiping AN ; Qiong ZHANG ; Jinjun CAO ; Aizhong LIU ; Huaqiang XI ; Haibo LIU
Journal of Medical Postgraduates 2015;(11):1189-1192
According to the practical problems including uneven distribution of medical resources in border areas , the difficulty of medical treatment for grass-roots unit, the low efficiency of referral between hospitals , and untimely mastery of health conditions of offi-ciers and soldiers , we constructed and developed a telemedicine information system based on hospital medical database with the backig of military integrated information network platform .A joint regional service platform among hospitals was constructed to realize high efficien -cy of medical referrals for officers and soldiers , computerization of personal medical information transmission , digitalization of health re-cords management , and paperless medical audit .The system provides convenient and high quality health care services , improves the level and efficiency of medical service , and enhances the ability of medical support to the troops by informationization .
2.Screening hv-S/TPK from TAC library of a Triticum aestivum-Haynaldia villosa translocation line.
Yulei SUN ; Aizhong CAO ; Xueming YANG ; Xiaoyun WANG ; Peidu CHEN
Chinese Journal of Biotechnology 2008;24(8):1327-1332
Hv-S/TPK gene, a resistance related gene to powdery mildew, was cloned by using genechip, and its expression was upregulated after the inoculation of Blumeria graminis to Haynaldia villosa. Using the specific primers of Hv-S/TPK to screen a genomic TAC (Transformation-competent artificial chromosome) library of translocation line 6VS/6AL, a positive TAC was screened. A 5-kb fragment containing Hv-S/TPK was subcloned and identified. This 5160-bp fragment (GenBank Accession No. EU153366) was determined by specific primer walking. The analysis of Hv-S/TPK genomic sequence showed three introns and four extrons between start code and stop code. In the promoter region of Hv-S/TPK, there were W-box and OCS-like elements which were the elements related to disease resistance. In this study, the positive TAC clone was used to as probe in situ hybridized to mitotic metaphase chromosomes of translocation line. The result of fluorescence in situ hybridization (FISH) indicated that the TAC clone containing Hv-S/TPK was from Haynaldia villosa chromosome.
Base Sequence
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Chromosomes, Artificial
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Cloning, Molecular
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Genomic Library
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Molecular Sequence Data
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Plant Diseases
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genetics
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Poaceae
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genetics
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Protein-Serine-Threonine Kinases
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genetics
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Serine
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genetics
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Translocation, Genetic
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Triticum
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genetics
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virology