[Objective] To establish a content determination method of Berberine hydrochloride in San Yu Ointment by HPLC.[Methods] RP-HPLC was performed to determine Berberine hydrochloride on a column of Agilent Zorbax Eclipse XDB-C8(4.6?150mm,5?m)at 20℃; Acetonitrile-0.1 % H3PO4 (50∶50)(Adding 0.1 g C12H25NaO4S to every 100 mL)was used as mobile phase,the flow rate was 1.0 mL?min-1.The detection wavelength was at 265 nm.[Results]A good linearity can be found in the range of 39.40 ~394.0 ng,and the regression equation was Y = 4.073?103x+2.248 ? 103 (r=0.9992).The recovery was between 97.9 %~100.9 %,and the average recovery rate was 99.4 %,RSD was 1.5 %.[Conclusion]The method is simple,rapid,accurate and available,which can be used to control the quality of San Yu Ointment.

Objective To investigate healthcare-associated infection(HAI)in different departments in a children’s hos-pital,and provide scientific basis for effective prevention and control of HAI in children.Methods By bed-side visiting and medical record reviewing,survey on HAI prevalence rate was conducted among hospitalized patients in a given day in 2012, 2013,and 2014 respectively,and surveyed data of three years were analyzed.Results HAI prevalence rates in 2012,2013, and 2014 were 6.52%,5.59%,and 5.85% respectively,there was no significant difference (χ2 =1.02,P =0.60);the average HAI prevalence rate in three years was 5.98%,there was significant difference in average HAI prevalence rate among different departments(χ2 =291.83,P <0.001 ),the top 4 departments of HAI prevalence rate was hematology (27.49%),cardiothoracic surgery (21.13%),neonatal intensive care unit (16.43%),and pediatric intensive care unit (12.76%);the main infection sites were lower respiratory tract (58.44%),upper respiratory tract (22.63%),and gas-trointestinal tract (6.58%);the main pathogens causing HAI was Klebsiella pneumoniae (n=32,28.32%),followed by Acinetobacter baumannii (n=9,7.96%),Escherichia coli (n=9,7.96%),and Pseudomonas aeruginosa (n=7,6.19%). Conclusion Strengthening prevention and control intervention of HAI in high risk departments has important significance to reduce the incidence of HAI in children’s hospital.

OBJECTIVETo determine the optimum compatibility extraction technology of Rhei Radix et Rhizoma, Scutellariae Radix and Phellodendri Chinensis Cortex.

METHODThe drugs were combined respectively firstly, then use HPLC to determinate extraction rates of evaluating indicators including emodin, chrysophano, baicalin and berberine hydrochloride. Orthogonal experiment was used to detect the effect on concentration and volume of ethanol, times of extraction.

RESULTThe extraction rates of evaluating indicators were relatively high in combined extraction of Rhei Radix et Rhizoma with Scutellariae Radix and single extraction of Phellodendri Chinensis Cortex. The optimized combined extraction were 12 times more than the volume of 60% ethanol solution, by boiling for 3 times, each for 1.0 hour, and that of single extraction were 10 times more than the volume of 60% ethanol solution, by boiling for 3 times, each for 1.0 hour.

CONCLUSIONThe optimized compatibility extraction technology is reasonable, stable and practical.

Chemical Fractionation ; methods ; Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; analysis ; isolation & purification ; Ethanol ; chemistry ; Phellodendron ; chemistry ; Scutellaria baicalensis ; chemistry ; Time Factors

Objective To compare the clinical characteristics of patients with non-valve atrial fibrillation(NAVF)and acute ischemic stroke(AIS) between two groups:aged ≥80 years versus 60-79 years.Methods One hundred and five inpatients with NVAF and AIS,treated in Neurological Department Beijing Hospital from November 2009 to November 2014,were divided into two groups of the very elderly patients (≥ 80 years,n =44) and old patients (60-79 years,n =61).Baseline data and clinical characteristics of patients with NAVF and AIS were retrospectively compared between the two groups to explore their risk factors,subtypes of AIS,the complications and prognosis.Results Age was the risk factor for stroke in the very elderly patients with NAVF.Compared with old patients group,the very elderly patients were associated with significantly higher baseline NIHSS(National Institutes of Health Stroke Scale) (11.59 ± 7.76 vs.6.03±7.12,t=3.80,P=0.000).The rates of mRS(modified Rankin Scale)≥3(poorer clinical turnover)1 or more month after AIS was 21.3％ in the old patients versus 56.8％ in the very elderly patients(x2 =13.96,P＜0.01).Moreover,anterior circulation infarction was the main subtypes of AIS in the very elderly patients versus old patients(x2 =12.28,P =0.020).Meanwhile,the very elderly patients versus the old patients had markedly higher rates of complications of AIS,such as pneumonia (65.9％ vs.27.9％;x2 =14.53,P ＜ 0.05),gastrointestinal bleeding(36.4％ vs.13.1％;x2 =7.84,P＜0.05),acute coronary syndrome or heart failure(34.1％ vs.11.5％;x2 =7.89,P＜0.05).Conclusions The very elderly patients versus the old patients show that risk factors for NVAF with AIS are more often the ageing and smoking,and that anterior cerebral circulation is more often affected.The degrees of NVAF and AIS severity are higher,the more complications are found,and the prognosis is worse.