ObjectiveTo investigate the nursing scheme to prevent infection,thromboembolism,accidental shedding during application of permanent deep venous catheter in hemodialysis. Methods28 patients undergoing maintenance hemodialysis from January 2007 to February 2009 were randomly divided into the observation group and the control group with 14 patients in each group.The control group took conventional care only,the observation group was given pertinent nursing intervention.After treatment the number of patients with infection and thromboembolism were compared in both groups. ResultsAll patients were successfully placed catheters.Number of patients with infection,thromboembolism and accidental shedding in the observation group were less than those in the control group.But there was no significant difference in average indwelling time of catheters between two groups. ConclusionsApplication of permanent deep venous catheter in hemodialysis shows good effect,it can decrease the incidence of complication and should be widely applied.

Purpose: To investigate the relationship between quantity of suppurative moxibustion and its effect on allergic asthma. Methods: The patients were randomly divided into suppurative moxibustion 9 cones group, 3 cones group and 6 cones group. The biggest area, scar-losing time, suppurating time and scarring time of post-moxibustion sore, and the clinical effects of each group were observed. Results: The biggest post-moxibustion sore area of 9 cones group was bigger than 3 cones group but had no difference with 6 cones group,and scar-losing time, suppurating time and scarring time of post-moxibustion sore of each group were of no difference. The clinical effects of 9 cones were better than 3 cones group but have no difference with 6 cones group. Conclusion: The quantity of suppurative moxibustion is in close association with the effects in the treatment of allergic asthma, and eventually becomes the key factor for good results.

OBJECTIVE:To explore the role of clinical pharmacists participating in the individualized treatment for purulent meningitis. METHODS:Clinical pharmacists participated in the therapy for a patient with purulent meningitis complicated with Staphylococcus aureus infection. According to patient's condition,clinical pharmacists assisted physicians to formulate preliminary therapeutic plan. Reviewing relevant guidelines,domestic and foreign literatures,clinical pharmacists suggested to combine with dexamethasone so as to relieve inflammatory reaction. According to the results of drug sensitivity test,based on vancomycin plasma concentration monitoring and population pharmacokinetics model fitting,clinical pharmacists suggested to reduce the dose of vanco-mycin to 0.5 g,ivgtt,q12 h. The pharmaceutical care were conducted throughout the therapy,including efficacy evaluation of an-ti-infective therapy,ADR monitoring,renal function monitoring,etc. RESULTS:Physicians adopted some suggestions of clinical pharmacists. The disease condition of the patient was recovred,and no ADR related to vancomycin was found. On the 16th day, the patient was discharged from the hospital. CONCLUSIONS:Clinical pharmacists participate in treatment of purulent meningitis, assist physicians to optimize therapy plan based on relevant guideline,literature,etiological examination,blood concentration moni-toring and pharmacokinetics model fitting results. It not only guarantee therapeutic efficacy of anti-infective therapy,but also pre-vent and reduce the occurrence of ADR.

Results obtained from current studies showed that the vitamin C (VC) content in Rose roxburghii Tratt was higher, about 1664-2601 mg/100g fresh sample, than all the fruits and vagetables. Vitamin E(VE) ranged from 2.4 to 3.6mg/100g sample was also higher than any other fruits and vegetables.After the wild plants were domestricated for 2 years, its VC content increased about 26%, Sugar increased 50%, tannin, however, decreased approximately 50%.

Objective:To investigate the relationship between fester capacity and clinical efficacy on allergic asthma treated by scarring moxibustion.Methods:The patients were randomly divided into scarring moxibustion 9-cone group,3-cone group and 6-cone group.The biggest area,scab-lost time,first-festering time and scarring time of moxibustion sore,and the clinical efficacy in each group was observed.Results:The biggest post-moxibustion sore area in 9-cone group was larger than that in 3-cone group,but had no difference in comparison with 6-cone group;the scab-lost time,first-festering time and scarring time of post-moxibustion sore in each group were of no difference.The clinical efficacy was better in 9-cone group than in 3-cone group,but had no difference in comparison with the 6-cone group.Conclusion:Fester capacity in treating allergic asthma by scarring moxibustion is related to the clinical efficacy and definite fester capacity is the key to good results on allergic asthma.

Objective: To investigate the effects of miRNA on the expression of paraoxonase 1 (PON1) and its clinical application in the patients with nonalcoholic steatohepatitis (NASH). Methods: Bioinformatics methods were used to analyze and predict PON1 related regulation on miRNA. PON1 luciferase reporter gene vectors were constructed and the activity of dual luciferase was analyzed. The up/down-regulated levels of miRNA in HepG2 cells of different groups were detected by real-time fluorescence quantitative PCR (qRT-PCR), and the levels of PON1 protein in HepG2 cells were detected by western blot. The levels of miR140-5p in the serum of healthy people and NASH patients were also analyzed by qRT-PCR. Results: According to the prediction of TargetScan database, miR140-5p may bind complementarily to the end of PON13′-UTR. The analysis for the activity of dual luciferase reporter gene showed that miR-140-5p mimic significantly downregulated the fluorescence of wild type PON1 vector (P＜0.01). The results of qRT-PCR demonstrated that miR-140-5p mimic group showed high overexpression (P＜0.01) compared with the normal cell control group and the negative mimic control group, while miR-140-5p inhibitor group appeared corresponding low expression (P＜0.05). western blot results suggested that the transfection of miR140-5p mimic significantly down-regulated the expression of PON1 (P＜0.01) while miR140-5p inhibitor up-regulated this expression (P＜0.01). Compared with the healthy control group, the level of miR140-5p was decreased in the serum of NASH patients, and the difference was statistically significant (P＜0.01). Conclusion miR140-5p may be involved in the progression of nonalcoholic steatohepatitis through regulation for the posttranscriptional gene expression of PON1.