Objective To observe the pre-thrombosis markers in patients of different body fluids and discuss the relations between body fluids type and prethrombosis state. Methods The expression of CD41, CD62p on platelets, the level of plasma tissue plasminogen activator (t-PA) and its inhibiter (PAI-1), endothelin (ET-1), activated partial thromboplastin time (APTT), fibrinogen (FIB), prothrombin time (PT) and thrombin time (TT) were tested by using flow cytometer, ELASA, radioimmunoassay method and auto coagulometer. Results Patients with Abnormal Savda syndrome occupy 65.96% and non Abnormal Savda syndrome occupy 34.04% in complex diseases. Compared with normal control group, the average expression of CD62p, the level of plasma PAI-1, ET-1, FIB both in Abnormal Savda syndrome and non Abnormal Savda syndrome groups were significantly increased (P

OBJECTIVETo study the correlation between prethrombotic biomarkers and traditional Uyghur medicinal syndromes of malignant tumor.

METHODSOne hundred and fifty-nine malignant tumor inpatients were randomly selected and typed according to traditional Uyghur medicine theories. The expressions of peripheral platelet membrane glucoprotein (CD41 and CD62p), levels of serum endothelin (ET-1), plasma tissue plasminogen activator (t-PA), plasminogen activator inhibitor (PAI), plasma fibrinogen (FIB), prothrombin time (PT), and thrombin time (TT), and activated partial thromboplastin time (APTT) were detected by flow cytometry, radioimmunoassay, and enzyme-linked immunosorbent assay (ELISA) respectively.

RESULTSThe patients were typed as 68 of abnormal Savda syndrome, 34 of abnormal Khan syndrome, 31 of the abnormal Sepra syndrome, and 26 of the abnormal Belghem syndrome. Compared with the control group, the levels of CD62p, PAI, and ET-1 increased, levels of FIB and t-PA decreased, PT prolongated and APTT shortened in the abnormal Savda group and the non-abnormal Savda group (including abnormal Khan, abnormal Sepra, and abnormal Belghem types) (all P < 0.05). No significant difference of CD41 or TT was shown in inter-group comparison (P > 0.05). Compared with abnormal Khan and Belghem groups, the ET-1 level increased in the abnormal Savda group (P < 0.05). Compared with the abnormal Sepra group, the CD62p positive percentage increased in abnormal Savda group (P < 0.05). No significant difference in CD41 positive percentage, t-PA or PAI-1 contents, PT, TT, or APTT was shown in patients of different traditional Uyghur medicine syndrome groups (P > 0.05).

CONCLUSIONSPrethrombotic changes existed in malignant tumor patients of different Uyghur abnormal syndrome types, manifested as injuries of vessel epithelial cells, platelet activation, increased blood viscosity, lowered fibrinolytic function. The prethrombotic changes were more obviously seen in the abnormal Savda group.

Adult ; Aged ; Case-Control Studies ; Female ; Humans ; Male ; Medicine, Chinese Traditional ; methods ; Middle Aged ; Minority Groups ; Neoplasms ; blood ; diagnosis ; Partial Thromboplastin Time ; Plasminogen Activator Inhibitor 1 ; metabolism ; Platelet Membrane Glycoproteins ; metabolism ; Tissue Plasminogen Activator ; metabolism

OBJECTIVE: To study the changes and significance of prethrombotic state indexes in patients with malignant cancer. METHODS: The expression of CD62p on platelets, level of plasma tissue plasminogen activator (t-PA), level of serum endothelin (ET-1), activated partial thromboplastin time (APTT), fibrinogen ( FIB), prothrombin time (PT), and thrombin time (TT) were examined by flow cytometer, enzyme-linked immunosorbent assay, radioimmunoassay method, and auto coagulometer. RESULTS: The average expression of CD62P, level of plasma FIB and serum ET-1 in the tumor group increased compared with those in the control group (P<0.05); the level of t-PA in the tumor group decreased compared with that in the control group (P<0.05). APTT and PT were significantly shortened in the tumor group (P<0.05). There was no significant changes of TT in 2 groups (P>0.05). CONCLUSION The platelet is activated, the thrombin activity increases, and the fibrinolytic function declines in patients with malignant cancer; there is an obvious prethrombotic state in patients with malignant cancer.
Adult ; Aged ; Blood Coagulation ; Blood Platelets ; metabolism ; Case-Control Studies ; Endothelin-1 ; blood ; Female ; Fibrinogen ; metabolism ; Humans ; Male ; Middle Aged ; Neoplasms ; blood ; P-Selectin ; metabolism ; Partial Thromboplastin Time ; Plasminogen Activator Inhibitor 1 ; blood ; Prothrombin Time ; Thrombin Time ; Tissue Plasminogen Activator ; blood