1.Significance of modified gold nanocages in photothermal therapy and radiosensitivity enhancement
Aiwei ZHANG ; Dexin YU ; Yafei QI ; Weihua GUO ; Xiangxing MA
Chinese Journal of Radiation Oncology 2016;25(4):407-411
Objective To investigate the significance of modified gold nanocages ( GNCs ) in photothermal therapy and radiosensitivity enhancement for murine mammary carcinoma 4T1 cells.Methods A molecule probe, CD44-PEG-GNCs, was constructed on the basis of GNCs.Inductively coupled plasma mass spectrometry ( ICP-MS) and transmission electron microscopy ( TEM) were used to analyze Au uptake by 4T1 cells.The cell counting kit-8( CCK-8) assay was used to determine the impact of the probe on the viability of 4T1 cells.The CCK-8 assay and the Hoechst/PI double staining were used to evaluate the photothermal killing effects of near infrared laser (NIR).The colony forming assay and the Hoechst/PI double staining were used to measure the viability of 4T1 cells after irradiation with 6 MV of X-ray.Results ICP-MS and TEM showed that 4T1 cells had an uptake of a large number of GNCs, in which the number of CD44-PEG-GNCs taken up by 4T1 was 3-4 times larger than that of PEG-GNCs.The cell toxicity assay revealed that in a certain range of concentration, GNCs showed little effect on the viability of 4T1 cells, which was reduced to 81.2%in 48 hours.The CCK-8 assay, the Hoechst/PI double staining, and the colony forming assay showed that after NIR irradiation and radiotherapy, large-scale apoptosis was induced in CD44-PEG-GNCs-contained tumor cells by photothermal therapy combined with radiotherapy, which had substantially better outcomes than other groups.Conclusions The GNC-based molecular probe actively targets 4T1 cells with little cytotoxicity, indicating that it enhances the sensitivity of tumor towards photothermal therapy and radiotherapy.
2.Features of computed tomography examination of esophageal submucosal hematoma
Aiwei ZHANG ; Haisheng ZHOU ; Guobao JIA ; Zhigang ZHOU ; Huanhai XU ; Qingdong CHEN ; Qiande QIU
Chinese Journal of Digestive Surgery 2014;13(8):654-657
Esophageal submucosal hematoma is rarely seen in clinical practice and its clinical presentations are untypical.Gastroscopy might injure the esophageal mucosa and cause bleeding.Computed tomography had the advantages of quick examination and formation of imaging,as well as non-invasiveness,which is helpful for the diagnosis and differential diagnosis of the esophageal submucosal hematoma.The clinical data of 9 patients with esophageal submucosal hematoma who were admitted to the Yueqing People's Hospital,the First Affiliated Hospital of Wenzhou Medical University,the First Affiliated Hospital of the Zhengzhou University and the Third People's Hospital of Wenzhou from July 2005 to February 2014 were retrospectively analyzed.The features of computed tomography examination of esophageal submocosal hematoma include the range of the esophageal submucosal hematoma exceeds 2 parts of the esophagus,the rip of the esophageal mucosa begins and ends at the physiological narrowing of the esophagus,thickening of the esophageal wall,stricture of the esophagus,annular thickening and eccentric thickening of the esophageal wall,crescent,spot or gas shadows of the esophageal cavity,the density of the hematoma decreases as the time passed by.These features of computed tomography are of great importance for the diagnosis of esophageal submucosal hematoma.
3.Molecular epidemiological analysis of tracing and analysis of Yersinia pestis strains isolated from two human plague outbreaks with unknown sources in Gansu Province
GUO Limin ; HE Aiwei ; XI Jinxiao ; WU bin ; WANG Dingsheng ; XU Daqin ; ZHANG Xiaoyan
China Tropical Medicine 2023;23(10):1077-
Abstract: Objective To conduct a molecular epidemiological tracing and analysis of Yersinia pestis strains isolated from two human plague outbreaks with unknown sources in Gansu Province, China. The results of this analysis would provide a basis for isolating and controlling the sources of Yersinia pestis. Methods The strains of Yersinia pestis isolated from two human plague outbreaks occurring on December 12, 2017, and September 27, 2019 were genotyped by the different region (DFR) and the clustered regularly interspaced short palindromic repeats (CRISPR). The repeat numbers of the variable number tandem repeat (VNTR) loci in the tested strains of Yersinia pestis were calculated by the multiple variable number tandem repeats analysis (MLVA), and the location of the phylogenetic tree of the tested strains was determined with the method of minimum spanning tree (MST) by the software BioNumerics 6.6. Results The strain of 20171212 lacked DFR01, DFR02, DFR03, DFR04, DFR13, DFR23, and the DFR type was identified as type 8. The space sequence of YPa was a1'-a2-a3-a4-a5-a6-a7-a35, the space sequence of YPb was b1-b2-b3-b4, the space sequence of YPc was c1-c2-c3, the gene cluster of CRISPR was Ca35', the genotype of CRISPR was 26'. MLVA clustering analysis showed that the strain clustered within in the cluster of Yuerhong pasture in Subei County and formed an independent branch. On the other hand, the strain of 20190927 lacked DFR01, DFR13 and DFR23, with the DFR type identified as type 1b. The space sequence of YPa was a1-a2-a3-a4-a5-a6-a7, the space sequence of YPb was b1-b2-b3-b4, the space sequence of YPc was c1-c2-c3, the gene cluster of CRSIPR was Ca7, the genotype of CRSIPR was 22 MLVA clustering analysis showed that the strain was located close to the cluster of Dangjinshan in Akesai County, and relatively distant from the cluster of Yuerhong pasture in Subei County. Conclusions The genotypes of strain 20171212 by DFR and CRISPR were consistent with the main genotypes of Y. pestis from Himalayana Marmota foci in Subei County, which confirmed that the human plague cases were naturally occurring locally. However, the strain gathered the cluster of Yuerhong pasture in Subei County, which indicated that the source of infection was not in Yanchiwan Town, but in the surrounding area of the Yuerhong pasture. The genotypes of strain 20190927 by DFR and CRISPR were in accordance with the main genotype of Y. pestis from Himalayana Marmota foci in Akesai County and were closer to the cluster of Dangjinshan in Aksai County than to
4.Dynamic changes of tear fluid matrix metralloproteinase-9 within 1 year after laser in situ keratomileusis.
Hongpei JI ; Aiwei CHEN ; Weiwei ZHANG ; Hong GU ; Zhiling ZHANG ; Juqin FU
Journal of Southern Medical University 2014;34(8):1079-1082
OBJECTIVETo investigate the dynamic changes of matrix metalloproteinase-9 (MMP-9) level in tear fluid within 12 months after laser in situ keratomileusis (LASIK).
METHODSTwenty-two myopic patients undergoing uneventful LASIK were enrolled in this study. Tear fluid samples were collected from the patients for measurements of MMP-9 level using Western blotting preoperatively, at 7 and 14 days, and at 1, 2, 3, 6, and 12 months after the surgery.
RESULTSMMP-9 concentrations in the tear fluid of post-LASIK patients showed a time-dependent variation pattern. MMP-9 reached its peak level in the tear fluid at 14 days postoperatively, which was 2.70 times the preoperative level; it gradually decreased thereafter but was still 1.38 times the preoperative level at 12 months after the surgery.
CONCLUSIONSMMP-9 concentrations in the tear fluid of post-LASIK patients show a time-dependent variation pattern and remains higher than the preoperative level even at 12 months after the surgery, suggesting that corneal wound healing after LASIK lasts for more than 12 months.
Cornea ; Humans ; Keratomileusis, Laser In Situ ; Matrix Metalloproteinase 9 ; chemistry ; Myopia ; surgery ; Postoperative Period ; Prospective Studies ; Tears ; chemistry ; Wound Healing
5.Adenocarcinoma of the rete testis: a case report
Junwei WANG ; Miaoyong YE ; Aiwei ZHANG
Chinese Journal of Urology 2022;43(6):460-461
Adenocarcinoma of the rete testis is a rare testicular malignant tumor with high invasiveness and poor prognosis. Due to the lack of specific tumor marker and characteristic features in imaging, early diagnosis of testicular reticulum adenocarcinoma is difficult, and a final diagnosis depends on the pathological and immunohistological examination. At present, there is no systematic treatment guidance, and radical orchiectomy is preferred. This article reported a 66-year-old patient with right adenocarcinoma of the rete testis who underwent right radical orchiectomy. He refused adjuvant radiotherapy or chemotherapy after surgery, and developed lung, liver, and retroperitoneal lymph node metastasis 8 months afterwards. He was followed up at 15 months, when he was died of multiple metastases.
6.Practice and exploration of training for clinical, teaching and scientific research ability of cardiovascular specialists
Fangfang WANG ; Aiwei LI ; Qi ZHANG ; Jiangli HAN
Chinese Journal of Medical Education Research 2022;21(5):584-587
Standardized training of specialists is an important part of medical education system. The training goal is to cultivate high-quality specialists with high clinical practice ability, teaching and scientific research ability. Based on the training practice of clinical, teaching and scientific research ability of the standardized training of cardiovascular specialists in Peking University Third Hospital, this paper discusses how to establish and improve the quality management system of cardiovascular specialist training, and introduces the training content, training methods, training organization and management, and training effect respectively, hoping to provide more practical basis for improving the quality of standardized training for cardiovascular specialists.
7. Report of antimicrobial resistance surveillance program in Chinese children in 2016
Chuanqing WANG ; Aimin WANG ; Hui YU ; Hongmei XU ; Chunmei JING ; Jikui DENG ; Ruizhen ZHAO ; Chunzhen HUA ; Yinghu CHEN ; Xuejun CHEN ; Ting ZHANG ; Hong ZHANG ; Yiping CHEN ; Jinghong YANG ; Aiwei LIN ; Shifu WANG ; Qing CAO ; Xing WANG ; Huiling DENG ; Sancheng CAO ; Jianhua HE ; Wei GAO ; Shuzhen HAN
Chinese Journal of Pediatrics 2018;56(1):29-33
Objective:
To analyze the antimicrobial resistance profile in Chinese children.
Methods:
This was a prevalence survey. From January 1 through December 31, 2016, the isolates were collected from 10 tertiary children hospitals in China. Antimicrobial susceptibility testing was carried out by routine laboratory methods. The penicillin susceptibility of
8. Clinical characteristics and antimicrobial resistance of pneumococcal infections from 9 children's hospitals in 2016
Chao FANG ; Xuejun CHEN ; Mingming ZHOU ; Yinghu CHEN ; Ruizhen ZHAO ; Jikui DENG ; Chunmei JING ; Hongmei XU ; Jinhong YANG ; Yiping CHEN ; Hong ZHANG ; Ting ZHANG ; Sancheng CAO ; Huiling DENG ; Chuanqing WANG ; Aimin WANG ; Hui YU ; Shifu WANG ; Aiwei LIN ; Xing WANG ; Qing CAO
Chinese Journal of Pediatrics 2018;56(8):582-586
Objective:
To describe the clinical characteristics of pneumococcal infections and drug resistance of
9.Distribution characteristics and drug resistance of carbapenem-resistant Enterobacteriaceae in chil-dren from 2016 to 2017
Bingjie WANG ; Fen PAN ; Hong ZHANG ; Ting ZHANG ; Hongmei XU ; Chunmei JING ; Chuanqing WANG ; Aimin WANG ; Hui YU ; Shuzhen HAN ; Aiwei LIN ; Shifu WANG ; Qing CAO ; Xing WANG ; Chunzhen HUA ; Yinghu CHEN ; Xuejun CHEN ; Jikui DENG ; Ruizhen ZHAO ; Huiling DENG ; Sancheng CAO ; Jianhua HAO ; Wei GAO ; Yiping CHEN ; Jinhong YANG
Chinese Journal of Microbiology and Immunology 2019;39(8):583-590
Objective To investigate the distribution and drug resistance of carbapenem-resistant Enterobacteriaceae ( CRE) isolated from children in China. Methods CRE strains were collected in 10 ter-tiary children's hospitals of China from January 1, 2016 to December 31, 2017. Antimicrobial susceptibility of the clinical strains was detected with disk diffusion method ( KB method) and automated method. The re-sults were analyzed according to the Clinical and Laboratory Standards Institute ( CLSI) Standards published in 2017. WHONET 5. 6 software was used to retrospectively analyze the distribution characteristics and drug resistance of these strains. Results A total of 3065 CRE clinical strains were isolated from children with an overall prevalence of 7. 7% and among them, 13. 5% were isolated in neonatal group and 5. 8% in non-neo-natal group. The detection rate of CRE in 2017 was higher than that in 2016 (9. 7% vs 5. 7%). Among the 3065 CRE strains, there were 1912 strains of Klebsiella pneumoniae (62. 0%), 667 strains of Escherichia coli (22. 0%), 206 strains of Enterobacter cloacae (7. 0%), 56 strains of Klebsiella aerogenes (1. 8%) and 47 strains of Serratia marcescens (1. 5%). Most of the strains were isolate in neonatology departments including neonatal intensive care units (NICU) and intensive care units (ICU), accounting for 44. 8% and 19. 7%, respectively. Respiratory tract (61. 8%), urine (19. 4%) and blood (5. 7%) specimens were the main sources of CRE isolates. Results of antimicrobial susceptibility test showed that the CRE strains were highly resistant to carbapenem antibiotics such as imipenem, meropenem and ertapenem, as well as penicillins and most cephalosporins (79. 6%-100%), especially those isolated in the neonatal group (P<0. 05). Children had relatively low resistance rates to aminoglycosides such as amikacin (19. 7%) and fos-fomycin (11. 9%), fluoroquinolones such as levofloxacin (37. 7%) and ciprofloxacin (43. 3%), and tige-cycline (3. 8%). Currently, no polymyxin B-resistant strains were isolated. Conclusions The prevalence of common CRE strains in children in 2017 was higher than that in 2016, especially in newborns. Drug re-sistance in CRE strains isolated from neonates to common antibiotics was more severe, suggesting that great attention should be paid to it and timely measures should also be taken.
10. A multicenter clinical study on 1 138 cases of invasive pneumococcal disease in children from 2012 to 2017
Liang ZHU ; Wenhui LI ; Xinhong WANG ; Kun TAN ; Qingfeng FANG ; Qingxiong ZHU ; Kangkang WU ; Qiaozhi YANG ; Aiwei LIN ; Huiling DENG ; Jing BI ; Jing LIU ; Shiyong ZHAO ; Yun LIU ; Shujun JING ; Yumin WANG ; Lianmei LI ; Qing ZHAO ; Kaihu YAO ; Xi WANG ; Li JIA ; Fang WANG ; Jikui DENG ; Jing SUN ; Chunhui ZHU ; Kai ZHOU ; Jun LIANG ; Xiuzhen NIE ; Sancheng CAO ; Dongmeng WANG ; Shuangjie LI ; Xuexia CHEN ; Juan LI ; Yi WANG ; Lan YE ; Yanhong ZHANG ; Fang DONG ; Zhi LI ; Yonghong YANG ; Gang LIU
Chinese Journal of Pediatrics 2018;56(12):915-922
Objective:
To explore the clinical features, the serotype distribution and drug resistance of the isolates in patient with invasive pneumococcal disease (IPD).
Methods:
By retrieving the laboratory information system in 18 children′s hospitals from 2012 to 2017, the children with IPD were enrolled.