AIM To study the chemical constituents from the dregs of Euphorbia Alatavica Boiss.METHODS The acetone-methanol extract from the dregs of E.Alatavica was isolated and purified by silica,Sephadex LH-20 and preparative-HPLC column,then the structures of obtained compounds were identified by physicochemical properties and spectral data.RESULTS Nine compounds were isolated and identified as 8-hydroxy-3-methoxy-5H-pyrido [2,1-d] pyrazin-5-one (1),isoscopoletin (2),dihydroapigenin (3),β-carboline alkaloid (4),syringaresinol (5),pinoresinol (6),methylparaben (7),methyl-3,4-dihydroxybenzoate (8),trimethyl3,4-dehydrochebulate (9).CONCLUSION All the compounds are isolated from this plant for the first time,compound 1 and 4 are isolated from the genus Euphorbia for the first time.

OBJECTIVEThe four major active compositions, namely esculetin, lactucin, lactucopicrin and chlorogenic acid in seed, stem and root of the Cichorium glandulosum Boiss. et Huet that planted in Xinjiang have been quantified by HPLC.

METHODHPLC method was used, with Inertsil ODS-SP column (4.6 mm x 250 mm, 5 pm). The flow rate was 1.0 mL x min(-1). The column temperature was set at 32 degrees C. The mobile phase was methanol--0.2% formic acid, 0-40 min, methanol 30%--70% gradients. Injection volume was 5 microL. The detecting wavelength were 256, 350, 299 and 229 nm, respectively.

RESULTThe percentage recoveries were 98.2%, 99.57%, 100.50%, and 99.46% for chlorogenic acid, esculetin, lactucin, and lactucopicrin, respectively. The correlation coefficients (r) were 1.000, 0.9989, 0.9998, 1.000 and RSD were 1.6%, 1.5%, 0.77%, 2.0% for chlorogenic acid, esculetin, lactucin, and lactucopicrin, respectively. The contents of the chlorogenic acid, esculetin, lactucin and lactucopicrin were 0.0048, 0.0043, 0.6789, 0.7520 mg x g(-1), respectively in the root, and 0.0710, 0.1890, 0.2396 and 0.0520 mg x g(-1) in the seeds of C. glandulosum, respectively.

CONCLUSIONThis method was sensitive, rapid and simple, with good linearity, recovery and reproducibility.

Asteraceae ; chemistry ; Chromatography, High Pressure Liquid ; Linear Models ; Organic Chemicals ; analysis ; chemistry ; pharmacology ; Plant Structures ; chemistry ; Quality Control ; Reproducibility of Results

OBJECTIVETo study on the new preparation technology of Hugan Buzure granule and to compare protective effect on liver injury in rats by different extraction processes.

METHODVolatile oil extraction technology, inclusion condition and ethanol extraction condition were selected by orthogonal experiments. The experiment models of liver injury were induced by carbon tetrachloride, bacillus calmette-guerin (BCG) and plus lipolysaccharides (LPS) in rats, respectively. ALT, AST in serum, and MDA, SOD in liver were measured and the rats were killed to calculate the liver coefficient to evaluate the protective effect of Hugan Buzure granule on experimental injury in rats.

RESULTThe optimum conditions of volatile oil extraction were 1:12 of solid-liquid ratio, 2 h of soaking time, and 8 h of extracting time. The optimal beta-cyclodextrin inclusion complex condition was as follows: the volatile oils formed complex with the beta-CD in a ratio of 1: 6 and stirring for 1 h at 40 degrees C. The optimum ethanol extraction was as follows: refluxing and extracting 3 times with 10-fold 50% ethanol, 2 h for each time. Compared with the model group, the new technology extraction of Hugan Buzure granule could obviously inhibite the elevation of serum ALT (P < 0.01), AST (P < 0.05) of liver injury induced by BCG + LPS, and elevate the contents of SOD, and obviously inhibite the elevation of serum AST (P < 0.01) of liver injury induced by carbon tetrachloride.

CONCLUSIONThe new preparation technology was feasible. The new extraction could protect the liver injury in rats, which was better than extraction of current preparation technology.

Animals ; Drugs, Chinese Herbal ; analysis ; pharmacology ; Liver ; drug effects ; Mice ; Oils, Volatile ; isolation & purification ; pharmacology ; Technology, Pharmaceutical

Objective To establish a method for quality evaluation of Nardostachyos Radix et Rhizoma derived from Nardostachys jatamansi DC by determining the contents of nardosinone and establishing fingerprints. Methods HPLC fingerprint and content determination methods were established to evaluate ten batches of Nardostachyos Radix et Rhizoma from Nardostachys jatamansi DC.For the determination of nardosinone, the samples were separated by Phenomenex-C18 ( 4. 6 mm × 250 mm, 5 μm) with the mobile phase consisting of acetonitrile and water at the flow rate of 0. 8 mL · min-1 , the detection wavelength was set at 250 nm,and column temperature was 25℃.The gradient mobile phase system was used for the fingerprints. Results HPLC fingerprint of Nardostachyos Radix et Rhizoma was established with good chromatography separation and repeatability, which could be used for quality control of Nardostachyos Radix et Rhizoma. After similarity analysis, the results showed that there was no significantly difference between the HPLC fingerprints of samples from different origins,but the content of four major peaks were different.The amount of nardosinone from ten batches of Nardostachyos Radix et Rhizoma was determined by HPLC-DAD, which showed that the contents of nardosinone from Nardostachys jatamansi DC were obviously different with each other. Conclusion The method is sensitive, repeatable and accurate. It can be used as a method of quality control for Nardostachyos Radix et Rhizoma.

Twelve alkaloids were isolated from the bulbs of Fritillaria yuminensis by column chromatography over silica gel, ODS, and Sephadex LH-20, as well as RP-HPLC. Their structures were identified mainly by NMR and MS analyses as yubeinine(1), imperialine(2), delavinone(3), tortifoline(4), hupehenizioiside(5), imperialine-β-D-glucoside(6), kuroyurinidine(7), pengbeisine A(8), walujewine A(9), peimisine-3-O-β-D-glucopyranoside(10), solanidine-3-O-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside(11), and solanidine-3-O-α-L-rhamnopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→4)]-β-D-glucopyranoside(12). Compounds 4-12 were obtained from F. yuminensis for the first time.
Alkaloids ; analysis ; Chromatography, High Pressure Liquid ; Fritillaria ; chemistry ; Magnetic Resonance Spectroscopy ; Molecular Structure ; Phytochemicals ; analysis ; Plant Roots ; chemistry

Seven aromatic glycosides (1-7), including four phenylethanol glycosides, one phenylmethanol glycoside, one phenylpropane glycoside and one benzoside, were isolated from the methanolic extract of Uighur Medicine Elaeagnus angustifolia flowers. Their structures were elucidated based on the analysis of spectroscopic data (1D, 2D NMR and HR-MS). Compound 1 is a new compound, named as angustifol A. Six known compounds were identified as 2-phenylethyl-O-β-D-glucopyranoside(2), salidroside (3), vanillic acid 4-O-β-D-glucopyranoside(4), vanilloloside (5), (Z)-isoconiferin (6), 2-phenylethyl-6-O-α-L-arabinofuranosyl-β-D-glucopyranoside (7). Compounds 2-7 were isolated from the genus Elaeagnus for the first time. In vitro anti-inflammatory assays revealed that none of these compounds showed good COX inhibitory activities.