To study the determination method of cholesterol in human serum by Coulometric titration. METHODS: The titration was carried out in an electrolytic solution containing 1 mol.L-1 potassium bromide and glacial acetic acid (1∶3). Bromine was electrogenerated at the anode and cholesterol reacted with bromine with a value of reactional electrons (n) as 2. RESULTS: The ranges of recovery for 0.115 mg to 0.459 mg of cholesterol were from 93.9% to 105%, and the average recovery was 100.3%. The relative standard deviation in all applied analysis was less than 1.5%. The accuracy of the method was also assessed in comparison with the results obtained by the enzymatic method used clinically. CONCLUSION: The method proposed is simple, rapid and accurate, and is suitable for the determination of cholesterol in human serum samples.

Objective:To explore influence of nursing intervention according to comprehensive geriatric assessment (CGA)on quality of life (QOL)in aged patients With coronary heart disease (CHD).Methods:A total of 96 aged CHD inpatients admitted in department of cardiology from Mar 2012 to Mar 2013 Were enrolled.According to num-ber table method,they Were randomly divided into routine nursing group (n=48,received specific nursing of cardi-ology department)and CGA group (n=48,received nursing intervention according to CGA based on routine nurs-ing).Chinese questionnaire of quality of life in patients With cardiovascular disease (CQQC)Was used to score and compare each dimension of QOL betWeen tWo groups at admission and on 6-month after discharge.Results:Com-pared With routine nursing group after intervention,there Were significant increase in scores of physical strength [(7.35±4.12)scores vs.(10.86±5.06)scores],patient's condition [(4.57±0.85)scores vs. (8.75±4.54) scores],medical condition [(4.72±0.82)scores vs.(5.90±0.40)scores],general living [(3.85±1.45)scores vs. (5.22±1.95)scores],social psychological conditions [(3.67±1.52)scores vs.(8.41±3.22)scores],Working sta-tus [(0.70±0.64)scores vs.(0.97±0.66)scores]and total score of CQQC [(24.86±4.43)scores vs.(40.11± 9.05)scores]in CGA group,P<0.01 or<0.05.Conclusion:Nursing intervention according to comprehensive geri-atric assessment can improve quality of life in aged patients With coronary heart disease.

Objective To explore the effects of the comprehensive geriatric assessment ( CGA) nursing model on the quality of life in elderly patients with coronary heart disease ( CHD ) .Methods A total of 143 cases of hospitalized elderly patients with CHD were randomly divided into two groups , from January 2012 to June 2013 .The control group ( 69 cases ) was given the cardiovascular specialty nursing and the CGA group (74 cases) was given the cardiovascular nursing based on the CGA nursing model .The scores of patients ’ physical strength, illness, medical condition, general life, social psychology, working and interpersonal relationship were surveyed using the questionnaire of quality of life in patients with cardiovascular diseases ( CQQC) when patients first admitted to the hospital and 6 months after discharge .Results In the CGA group , 75.7%patients had two or more kinds of elder problems , within which 67.6% was depression, 66.2% was sleep disorder, 62.2% were hearing disability and 60.8% was visual disability.Before the intervention, the scores of quality of life between groups had no significant differences (P >0.05).After the nursing intervention, the scores of physical strength , illness, medical condition, general life, social psychology, working and interpersonal condition in the CGA group were (10.86 ±5.06), (8.75 ±4.54), (5.90 ±0.40), (5.22 ±1.95), (8.41 ±3.22), (0.97 ±0.66) and (40.11 ±9.05).Those scores in the control group were (7.35 ±4.12), (4.57 ±0.85), (4.72 ±0.82), (3.85 ±1.45), (3.67 ±1.52), (0.70 ±0.64) and (24.86 ±4.43).The differences of scores between groups were significant ( t =4.541, 7.545, 11.137, 4.720, 11.095, 2.470, 12.653, respectively;P <0.05 ).Conclusions Based on the comprehensive evaluation, the CGA nursing model can play a positive role in clinical nursing of elderly CHD patients , and it also can improve the quality of life in elderly patients with CHD .