Objective To compare the changes in fibrinogen (Fib), C-reactive protein (CRP) and left atrial (LA) size between patients with atrial flatter (AFL) and those with atrial fibrillation (AF) to explore the relations among them. Methods We selected 53 AF patients (including 16 cases of paroxymal AF, 13 of persistent AF and 24 of permanent AF) and 18 patients with AFL; the control group consisted of 32 cases of sinus rhythm (SR). In all the patients, ECG or Horter and UCG were conducted; Fib and CRP were measured. The levels of the above indexes in AF group, AFL group and subgroups were compared with each other, and with those in the control group. Correlation analysis between CRP and LA size was made. Results Fib, CRP and LA size in AF group were significantly different from those in AFL and SR groups, but did not differ between the latter two groups. So did other parameters among the three groups. CRP in persistent AF and permanent AF differed significantly from that in AFL, SR and paraxymal AF. LA size in the groups of persistent AF differed from that in SR group, but there was no difference between those in persistent AF and AFL groups. LA size in permanent AF was significantly different from that in AF, AFL and SR. Positive significant linear correlations were found between CRP and LA size in AF (r=0.33). Conclusion Hypercoagulable state exists in AF; the AF. Positive correlation exists between LA size and inflammatory reaction; there is no hypercoagulable state in AFL.

Objective: To explore the clinical and magnetic resonance imaging (MRI) features of papillary musclehypertrophic cardiomyopathy. Methods: Our research contained 2 groups: Papillary muscle hypertrophic cardiomyopathy group,n=21 patients treated in our hospital from 2013-01 to 2015-12 including 18 male and 3 female; Control group,n=50 subjects without cardiovascular disease those were conifrmed by our hospital at the same period of time. Clinical and MRI examinations were conducted in all patients, the ifndings were compared between 2 groups. Results: Compared with control subjects, papillary musclehypertrophic cardiomyopathy patients had the main symptoms of shortness of breath, chest tightness and pain; associated with systolic murmur; ECG could be normal or with T wave inversion; cardiac MRI showed that 1/2 papillary muscle diameter>1.1cm. Blood levels of triglyceride, left atrial diameter, inter-ventricular septum thickness, the values of E/A and EDT were statistically different between 2 groups, allP<0.05. Conclusion: Clinical features of papillary muscle hypertrophic cardiomyopathy were lack of speciifcity, the morbidity and clinical signiifcance should be further investigated.

Objective To investigate the influence of high-fat diet on blood pressure and metabolism in Sprague-Dawley(SD) rats and its mechanism.Methods Forty male SD rats were randomly divided into 4 groups which were fed with a diet containing 53% calorie as fat(HF) or a normal diet(ND) for 5 or 10 weeks.Systolic blood pressure(SBP),body weight,abdominal adipose tissue,blood lipids,fasting insulin(FINS),and fasting plasma glucose(FPG) were measured after 5 and 10 weeks respectively.Results SBP of HF groups were higher than that of ND groups [HF5 vs.ND5,(105.506?4.634)mmHg vs.(100.060?4.773)mmHg,P

Objective To understand morphological characteristics of sinoatrial node cells(SNCs) by taking purified isolation and culture of neonatal rat's SNCs in vitro and to understand the expression characteristics of surface antigens hyperpolarization activated cyclic nucleotide gated cation channel 4(HCN4) and connexin 45(Cx45) on SNCs.Methods The SNCs were isolated from sinus node tissues removed from neonatal rats by trypsin and purified cultured with differential attachment and 5'-bromodeoxyuridine(BrdU) treatment.Results Three different types of cells were observed among the purified cultured SNCs: spindle,triangular and irregular cells.The spindle cells took up the greatest proportion,had the fastest beats,and had less developed organelle and scarce muscular fibrils.Immunofluorescence staining was positive against HCN4 and Cx45 on the spindle cells.Small amounts of HCN4 was observed on triangular cells.Conclusion ① Isolation by trypsin combined with differential attachment and BrdU treatment is a reliable approach to culturing sinoatrial node cells.② The spindle cells with the fastest beats and positive expressions of HCN4 and Cx45 may be the sinus node pacemaker cells.

AIM: To investigate the time window of bone marrow stem cell homing to the inchemia myocardium. METHODS: 110 SD male rats were randomly divided into four groups: GM-CSF-treated group (n=40) and sham-treated group (n=15) were given GM-CSF (50 ?g/kg/d) for 5days. The control group (n=40) and its sham-treated group (n=15) was injected equal volume of saline, acute myocardiar infarction were induced by LAD ligation in each group. At 1, 3, 5, 10 days in each group, the homed bone marrow stem cells were detected by expression of c-kit with immunohistochemical methods. Cardiac performance and pathological changes were examined at 28 days. RESULTS: At 28 days, both systolic function and diastolic function in GM-CSF-treated group were significantly higher than those in control group (P

6 score group using Wagner's QRS scoring system. CONCLUSIONS: Serum levels of certain inflammatory markers have some diagnostic value for ACS. The high levels of serum SAA, IL-6 and sCRP are not mainly caused by myocardial death, but by the inflammation in the multifocal unstable plague. [

Objective To investigate the effect of oleanolic acid (OA) on apoptosis, correlation between apoptosis and intracellular calcium, and its mechanism in human lung adenocarcinoma cell line A549. Methods Human lung adenocarcinoma A549 cells were incubated in vitro and assigned with OA concentrations of 0, 10, 20 and 40μg/mL. The apoptosis status of A549 cell line was detected with Annexin V-FITC/PI by flow cytometry (FCM); fluorescence intensity (FI) of A549 cells was assessed and the level of intracellular calcium was calculated at 24 hour of OA intervention. The relation between apoptosis and calcium FI was illustrated by curve fitting. Results FCM showed that 10, 20 and 40μg/mL of OA could induce A549 cell apoptosis, which followed a concentration-effect pattern; 24-hour intervention with 20μg/mL and 40μg/mL OA showed increased A549 cell apoptosis, and was significantly different from that with 0μg/mL OA (P<0.01). The FI of intracellular calcium concentration in 10, 20 and 40μg/mL OA groups was significantly higher than that in 0μg/mL group after 24 hours' intervention, and the FI showed a trend of increase with increased OA concentration (P<0.01). Curve fitting showed a significant correlation between apoptosis rate and intracellular calcium concentration in A549 cells (r=0.981, P<0.01). Regression equation was Y=0.508X-1.627. Conclusion OA plays a role in inducing apoptosis of human lung adenocarcinoma cells in a concentration-dependent manner. The OA-induced apoptosis is responsible for intracellular calcium overload of the tumor.

Objective To research whether serum hepatocyte growth factor (HGF) level increases in ischemic stroke and hemorrhagic stroke, and explore the relationship between the serum HGF level and stroke recurrence. Methods We studied a total of 92 consecutive acute stroke patients who had been admitted to hospital within 24h of onset from 6 participating hospitals in Xi'an from January 2000 to May 2004. All patients were divided into ischemic stroke group and hemorrhagic stroke group according to the results of brain computed tomography (CT) scan or MRI on admission. Patients in stroke groups were divided into recurrent group and non-recurrent group. Healthy volunteers or patients without cerebrovascular diseases comprised the control group. Stroke and control groups were strictly matched with 1∶1 ratio. The patients were followed up for 4 years. Serum HGF was tested with enzyme-linked immunosorbent assay (ELISA). Results Serum HGF of stroke patients was significantly higher than that of control group (P<0.05). The serum HGF level in recurrent group was higher than that in non-recurrent group of ischemic patients, and there was no significant difference in hemorrhagic ones. Conclusion These results indicate that serum HGF may be used as a diagnostic marker for stroke, and serum HGF level is helpful in predicting the recurrence of ischemic stroke.

Objective To study the serum iaminin (LN) and fibronectin (FN) changes in acute coronary syndromes (ACS), and explore the role of them in assessing the severity of ACS. Methods This study included 46 ACS patients [25 with acute myocardial infarction (AMI) and 21 with unstable angina (UA)], 51 stable angina (SA) patients and 47 people without CHD as controls. Serum levels of LN, FN, fibrinogen and blood fat were assessed. Coronary angiography were performed on 49 of them. Results The serum concentration of LN was lower in ACS patients [(85.20±27. 57)ng/mL], higher in SA patients [(116. 80 ± 28. 80)ng/mL] as compared to that in the control group [(100.06±29.96)ng/mL], with significant difference among the groups (P<0.05). No difference was found in FN among the three groups. However, the subgroup analysis in the group with ACS showed that the serum concentration of FN was significantly higher in UA patients [(229.60±121.39)μg/mL ], and lower in AMI patients [(108.31±47.12) μg/mL ]. The serum LN and FN concentration could respectively enter the logistic regression equations of ACS patients and US patients. Neither LN nor FN concentration was correlated with narrowing of coronary artery of angiography. Conclusion Serum LN and FN level may be a useful indicator for stability of atherosclerosis plaque in coronary arterial disease patients, but could not predict the extent of narrowing in coronary angiography.

Objective To examine the change of body weight (BW) and blood pressure (BP) in obese rats, clarify relationships between BP and BW and other factors. Methods Male Spraque-Dawley rats were fed either with normal diet (ND) or high calorie diet (HC) for 20 weeks. BW and BP of tail artery were observed biweekly and tetraweekly respectively; serum leptin and fasting insulin (FINS) were detected by enzyme-linked immunoadsordent assay (ELISA) and radioimmunoassay (RIA) respectively. Fasting plasma glucose (FPG) and free fatty acid(FFA) were measured by conventional means. Results BW, abdominal fat weight (AFW), ratio of abdominal fat weight to body weight (RF/W), systolic blood pressure (SBP), diastolic blood pressure (DBP), serum levels of leptin and FINS, FPG, FFA increased in the HD group after 20 weeks diet intervention (P＜0.05 or P＜0.01). SBP was strongly correlated with BW, leptin, FINS and FFA (P＜0.05), DBP was correlated with FFA (r=0.47, P＜0.05). In addition, leptin was positively correlated with BW, AFW, RF/W, FINS and FFA (P＜0.05 or P＜0.01). Conclusion In this study of high calorie-diet induced rats, the gain of BW is accompanied by increased BP. The obese rats have hyperleptinemia, hyperinsulinemia, hyperglycemia and dyslipidemia which may have important effects on the development of obesity-related hypertension. RF/W is the key factor in which affect serum leptin level.