Objeetive To investigate the retired elderly patients who had traditional Chinese medicine for preventing cardio-vascular disease and their complications for providing the best possible care.Methods Forty-eight retired elderly patients who had been given the infusion of traditional Chinese medicine for preventing the cardio-vascular disease over two years were analyzed.Results The finance,individual choice and social influences were the three major factors for the retired elderly people to choose traditional Chinese medicine for preventing cardiovascular diseases.In forty-eight patients of the study,100％ of them had secure medical insurance and they did not have any worries with the finance.93.75％ of them positively agreed with the treatment.91.67％ of them supported the idea use of the traditional Chinese medicine to promote the health and had the positive influence on the society.Conclusion Financial reasons,individual preference and social influences are the main factors to affect the retired elderly patient's choice of having traditional Chinese medicine to prevent cardiovascular disease.Our health care system for the retired elderly people has ensured the compliance of the treatment.With the increased awareness of the importance of the health,the traditional Chinese medicine has been widely used to prevent the occurrence of the cardio-vascular disease.

Objective To explore the curative effect of bisoprolol fumarate combined with wenxin granule for patients with heart failure complicated with premature ventricular.Methods 132 patients with heart failure and premature ventricular complexes were enrolled in our hospital from January 2013 to January 2016,and which patients divided into two groups,bisoprolol fumarate group (Group BF,n =66) accepted bisoprolol fumarate treatment (5 mg/time,1 times/day),and bisoprolol fumarate combined with wenxin granule group (Group BW n =66) adopted wenxin granule (9 g/time,3 times/day) based on the patients in Group BF.After 4 weeks treatment,the curative effect of all patients were evaluated with electrocardiogram monitor and cardiac ultrasound examination,and the plasma NT-proBNP concentration and hemorheology indexes were analyzed compared with pre-treatment.Results The recovery of ventricular function in Group BW was better significantly than that patients in Group BF from the cardiac ultrasound examination (P ＜ 0.05);The total relieved effective of clinical symptom,premature ventricular complexes and heart failure in Group BW were more remarkable than those in Group BF,and which difference from them was significance (P ＜ 0.05);The blood viscosity,plasma viscosity,plasma fibrinogen and plasma NT-proBNP concentration in patients with Group BW were significantly lower than those Group BF (P ＜ 0.05).Conclusions The clinical curative effect of bisoprolol fumarate combined with Wenxin Granule for heart failure complicated with premature ventricular was more remarkable compare with bisoprolol fumarate single treatment,and which possessed the relative safety.

Objective To study the clinical efficacy and safety of Rosiglitazone (RSG) combined with insulin in treatment of elderly type 2 diabetes mellitus (type 2 DM) that had poor response to insulin treatment. Methods 68 cases of elderly type 2 DM patients with poor response to insulin treatment were randomly divided into experimental group and control group.The control group was treated with proper dose of insulin,and the experimental group were administered RSG 4mg/day plus insulin for 20 weeks. 4 weeks and 20 weeks after treatment FBC, PBC, HbA1C, UAER of 24 hrs, daily changes of insulin dosage and side-effect of the medicine were observed. Results In the experimental group the levels of FBG and PBG decreased in 4 weeks(P

Objective To investigate the mechanism that cause allelic dropout of amelogenin gene on X chromosome(Amel-X)when using routine Sullivan106/112 bp primer set in sex identification and discuss its influence on the forensic sex identification and the clinical diagnosis.Methods Amel-X dropout was validated with Sullivan212/218 bp and Haas-Rochholz80/83 bp primer sets.Amplification of amelogenin gene was used to analyze dropout of the Amex-X followed by sequencin.Results Sullivan212/218 bp and Hgas-Rochholz80/83 bp primer sets could be used to identify gender correctly.Three types of point mntation were observed in the forward primer binding region of the Sullivan106/112 bp primer set by sequencing in the lost Amel-X,including single point mutation at 2nd and 13th sites,respectively,and heterozygous multiple point mutations at 2nd and 13th sites.Conclusions Point mutation in the primer binding region may result in a failure to amplify amelogenin allele and thus lead to a null allele.This finding should be mid attention to because it may interfere with the sex identification.

Objective:To investigation the veracity of orientation on modified upright lateral cephalometric radiographs. Methods:Fifty upright lateral cephalometric radiographs were analysed randomly.Twenty-three subjects,who had standard radiographs, were taken with modified technique. The chracterics both of radiographs were compared. Results:The orientations of porion?FH plane and the superimpositions of left and right gonion point on modified standard radiographs were more veracity. Conclusion:The veracity of orientation on upright lateral cephalometric radiographs can be improved.

Objective To compare the difference of ultrasound-guided anterior sciatic nerve block by multiple injection and single injection.Methods Among 90 patients undergoing lower limb operation,aged 18-68 years old,47 patients were males and 43 patients were females,all falling into ASA Ⅰ-Ⅲ.The patients were randomized to ultrasound-guided anterior sciatic nerve block by multiple injection (group M,n=45) single injection (group S,n=45).Local anesthetic of 0.5% ropivacaine hydrochloride 20 ml was used.The width,thickness and depth of sciatic nerve and blocking performance time were recorded.Sensory and motor blockade on the operated limb were evaluated every 10 minutes after injection of local anesthetic for 30 minutes and complications were observed in two groups.Results The width,thinkness and depth of sciatic nerve on the ultrasound screen were not statistically significant in the two groups.The performance time of group M was longer than that of group S (P<0.05).The proportion of patients with sensory blockade of each nerve significantly differed between the two groups: for superficial peroneal nerve at 10,20,and 30 minutes,for the sural nerve at 20,30 minutes,for the tibial nerve at 30 minutes,that of group M was higher than that of group S (P<0.05).A significantly greater rate of motor blockade was observed for the dorsal flexion of foot and toes extension in group M than that of group S at 30 minutes (P<0.05).The complications of nausea,vomitting and tinnitus were not different in two groups.Conclusion Multiple injection needs more operation time compared with single injection,but its onset time of sensory and motor blockade is quicker,the complications were not different.

Background Chinese dietary reference intakes for calcium are largely based on foreign studies.We undertook metaregression to estimate calcium requirements for Chinese adults derived from calcium balance data in Chinese adults.Methods We searched PubMed,Cochrane CENTRAL,and SinoMed from inception to March 5,2014,by using a structured search strategy.The bibliographies of any relevant papers and journals were also screened for potentially eligible studies.We extracted a standardized data set from studies in Chinese adults that reported calcium balance data.The relationship between calcium intake and output was examined by an individual participant data (IPD) and aggregate data (AD) meta-regression.Results We identified 11 metabolic studies in Chinese adults within 18-60 years of age.One hundred and forty-one IPD (n=35) expressed as mg/d,127 IPD (n=32) expressed as mg·kg body wt-1·d-1,and 44 AD (n=132) expressed as mg/d were collected.The models predicted a neutral calcium balance (defined as calcium output (Y) equal to calcium intake (C)) at intakes of 460 mg/d (Y=0.60C+183.98) and 8.27 mg·kg body wt-1·d-1 (Y=0.60C+3.33)for IPD,or 409 mg/d (Y=0.66C+139.00) for AD.Calcium requirements at upper intakes were higher than that at lower intakes in all these models.Conclusion Calcium requirement for Chinese adults 18-60 years of age approximately ranges between 400 mg/d and 500 mg/d when consuming traditional plant-based Chinese diets.

OBJECTIVE: To explore the correlations between symptom-based principal factors of rheumatoid arthritis (RA) and the effect of traditional Chinese medicine or Western medicine on RA after factor analysis of RA symptoms. METHODS: Four hundred and thirteen RA patients from 9 clinical centers were included in the clinical trial. They were randomly divided into Western medicine (WM) treated group with 204 cases and traditional Chinese medicine (CM) treated group with 209 cases. A complete physical examination and 18 common clinical manifestations were recorded before the randomization and after the treatment. The WM therapy included voltaren extended action tablet, methotrexate and sulfasalazine. The CM therapy included glucosidorum Tripterygll totorum tablet and Yishen Juanbi Tablet. The American College of Rheumatology 20 (ACR20) was used for efficacy evaluation. All data were analyzed on SAS 8.2 statistical package. Eighteen symptoms in the RA patients were analyzed by factor analysis and the relationships between the factors and effects were analyzed with Chi-Square test. RESULTS: Four principal factors were obtained from the analysis results of the 18 symptoms. The factors could represent the symptoms related to joints, cold-syndrome, deficiency syndrome and heat-syndrome in traditional Chinese medicine (TCM), respectively. The effect of WM therapy was better than CM therapy. After 12 weeks of treatment, the effect of CM on patients without deficiency-syndrome was better than the patients with deficiency-syndrome. After 24 weeks of treatment, WM therapy showed better effect on patients with cold syndrome than patients without cold syndrome. CONCLUSION: The results based on the factor analysis of RA symptoms are similar to the results of syndrome differentiation of TCM, and the factor-related different categories of symptoms are associated with the curative effect, thus further research on the symptoms is necessary.

Objective To initially map the gene responsible for autosomal dominant familial IgA nephropathy of a Chinese family by exclusive the five loci that had been reported with linkage analysis.Methods The genetic pattern of the familial IgA nephropathy was identified and the genomic DNA was extracted from the blood samples collected from the family members.Short tandem repeat (STR) inside the loci that had been reported was selected,such as 2q36,3p23-24,4q26-31,6q22-23,17q12-22,and the data with two-point linkage analysis were performed.Results Autosomal dominant inheritance pattern was demonstrated in phenotypes of the family and there was no linkage relationship in the above five loci of chromosomes because the maximum two-point LOD score was 0.39 at D17S1868.Conclusion Following exclusion of the loci which had been reported,there are other new pathopoiesis loci of FIgAN and it reveals that FIgAN has the genetic heterogeneity according to initial result at the same time.