Objective To investigate risk factors of infections in hepatic cancer patients after partial hepatectomy so as to provide theo-retical basis for the prevation of infections after the hepatecomy. Methods A total of 210 patients who underwent partial hepatectomy from December 2007 to December 2012 were divided into the infection group and the non-infection group,and they were retrospectively reviewed. Multivariate analysis was performed with logistic regression test. Results Totally 30 patients were infected after operation,and the incidence of infection was 14. 3%. There were singinficant difference between age,operation time,blood loss,blood transfusion and cirrhosis in the two groups(P<0. 05). Multivariate logistic regression analysis showed that the age,operation time,blood loss,blood transfusion and cirrhosis were significant risk factors for the infections after partial hepatecomy. Conclusion Aged over 60 years,operation time more than 5 hours, blood loss more than 3 000 mL,blood transfusion and cirrhosis were the key risk factors for the infections after partial hepatecomy.

OBJECTIVE Using bronchial asthma rats, to observe the effects of Smooth wheezing effect of polysaccharide. In addition, to study the effects of COM in different bronchial asthma model. METHODS Bronchial rats established by ovalbumin (OVA), were randomly divided into different group.Every other week building reference literature intraperitoneal injection of OVA, 21 d after injection of 3 consecutive ultrasonic atomization inhalation of 1% OVA stimulating 30 d,stimulate the 31 d began to medicine.Lavage for 4 weeks ELISA test ratio of IgE,SP-A,IL-4 and IL-5 was serum and bronchoal veolar lavage fluid etc.Data was in x ± s tabular format,SPSS16.0 statistics software is used to perform statistical analysis on the data, and P<0.05 shows meaningful statistical difference. RESULTS Quince polysac-charide can reduce the IgE,IL-4 level and elevated the SP-A,IL-5 level in serum and bronchoal veolar lavage fluid.CONCLUSION Quince polysaccharide has antiasthmatic effect on bronchial asthma rats.

OBJECTIVE 1H-NMR-based metabolomics approach was conducted to holistically explore the effect and mechanisms of Cydonia oblanga Mill flavonoids(COMF)on high-fat diet induced Atherosclerosis (AS) apoE-/- mice. METHODS AS model was established on the apolipoprotein e knockout mice by high-fat diet. The ApoE-/- mice were split into 6 groups including control group, AS model group,COMF High dose(COMF-H)group,COMF medium dose(COMF-M)group,COMF Low dose (COMF-L) group and Simvastatin group as the positive control group. Serum samples from all groups were analyzed by 1H-NMR technology and the OPLS-DA was conducted to distinguish the metabolic phenotypes. RESULTS Compared to the control group, serum levels of cholesterol, VLDL, leucine, isoleucine, valine, blood lipid, citrulline, methylamine, glucose, glycine, glycerol, myo-inositol, fructose, phenylalanine, unsaturated lipid, urea and other metabolites content significantly increased, while HDL, lactate, alanine, glutamate, glutamine, pyruvate, carnitine, citrate, choline content signifi-cantly decreased and the difference was statistically significant (P<0.05). The trend of metabolites in serum samples of COMF low, medium and high group was opposite to that of atherosclerosis model group and the difference was statistically significant(P<0.05).CONCLUSION Through functional analysis of these biomakers,amino acid metabolism,lipid metabolism,cholesterol metabolism,energy metabolism and inflammation reaction were considered as the most relevant pathological biomakers in the serum of AS mice.This study also demonstrates that COMF had the therapeutic effectiveness on AS through partly reversing the lipid,cholesterol,amino acid,energy metabolism and Inflammation reaction.

OBJECTIVE To observe the effects of serum metabolites by using 1H-NMR-based metabonomic approach to explore the possible mechanisms of total flavonoids in Ocimum Basilicum Linn (OBL) on atherosclerosis in apolipomtein E knockout (ApoE-/-) mice. METHODS Six-week-old male apoE knockout mice were divided into four groups(n=10)and fed with high fet diet:model,Simv-astatin, OBL-H, OBL-M and OBL-L groups. The homogeneous male mice of C57BL/6J were used as the normol group and fed with normal chow diet. After 14 weeks,1H-NMR technology was used to ex-plore the variability of serum metabolites by the method of PLS-DA and OPLS-DA. RESULTS Com-pared with normal group,Model group showed a significant increase in the serum levels of VLDL,LDL, β-hydroxyisobutyrate,lactate,myo-inositol and showed a significant decrease in the serum levels of al-anine, glutamine, proline, carnitine, methylamine, citrate, creatine, choline, taurine, pyruvate, β-glu-cose, α-glucose, glycine, lysine. Combined with model group OBL-H, OBL-M, OBL-L groups showed the effects of regulating the levels of different metabolites of the glucose,lipid and amino acid metabo-lism.CONCLUSION The anti-atheros-clerotic activity of total flavonoids in Ocimum Basilicum Linn may be related not only to regulation of lipid metabolism,but also glycometabolism and amino acid metabolism.

Objective To evaluate the incidence and influencing factors for post-operative healthcare-associated in-fection(HAI)in Uyghur patients undergoing cardiothoracic surgery in a hospital.Methods Clinical data of Uyghur patients hospitalized in the department of cardiothoracic surgery in a hospital of Kashgar region between June 2010 and June 2014 were collected,incidence of post-operative HAI was surveyed.Influencing factors for post-operative HAI were analyzed by univariate and multivariate logistic regression models.Results A total of 779 patients’clini-cal data were collected,53 patients (6.80%)developed post-operative HAI.The major infection sites were lower respiratory tract (2.44%),digestive tract(1 .28%),urinary tract(1 .16%),upper respiratory tract(1 .03%),and surgical sites(0.51 %).Multivariate logistic regression analysis showed that history of diabetes,body mass index (BMI)≥30 kg/m2 ,duration of operation ≥ 160 minutes,and invasive procedure were risk factors for post-opera-tive HAI in Uyghur patients undergoing cardiothoracic surgery.Conclusion Incidence of post-operative HAI in Uy-ghur patients undergoing cardiothoracic surgery in this hospital is high,risk factors for HAI are history of diabetes, high BMI,long duration of operation,and invasive procedure.

BACKGROUND:Pneumatic lithotripsy and holmium laser lithotripsy for distal ureteral calculi have their own advantages and disadvantages in efficacy and complications, but both of them are currently better treatments. ＜br> OBJECTIVE:To compare the efficacy and safety of holmium laser lithotripsy and pneumatic lithotripsy for the treatment of distal ureteral calculi through a meta analysis. ＜br> METHODS:A online search of China Biological Medicine disk, PubMed, Cochrane Library, Springerlink, Medline, and Embase databases was performed from March 1999 to December 2013. A manual retrieval of relevant journals was also done for clinical trials about holmium laser lithotripsy and pneumatic lithotripsy for treatment of distal ureteral calculi. RevMan 5.1.5 software (Cachrane Library) was used for meta analysis. The fol owing indexes were used to compare the results:mean operative time, early stone free rate, stone migration rate, ureteral perforation rate, postoperative hematuria rate and retaining double-J catheter rate. ＜br> RESULTS AND CONCLUSION:Four prospective studies (a total of 275 patients) were enrol ed, and there were 140 cases in the group of holmium laser lithotripsy and 133 cases in the pneumatic Lithotripsy group. Holmium laser lithotripsy conveyed significant benefits compared with pneumatic lithotripsy in mean operative time [WMD=-16.38, 95%CI (-20.29,-12.47), P＜0.000 01], stone migration incidence [OR=0.25, 95%CI (0.10, 0.63), P=0.003], retaining double-J catheter rate [OR=0.44, 95%CI (0.25, 0.78), P=0.004], while early stone free rate, postoperative hematuria rate and ureteral perforation rate had no significant changes between the two groups. Compared with pneumatic lithotripsy, holmium laser lithotripsy can shorten mean operative time and reduce the stone migration rate. Because of the lack of prospective studies and smal sample size, large-sized randomized control ed studies are urgently needed to provide evidence.

BACKGROUND:There are few genetic data related to nonsyndromic cleft lip and palate in Xinjiang Uygur population. OBJECTIVE:To investigate the relationship between interferon regulatory factor 6 (IRF6) and non-syndromic cleft lip and palate in Xinjiang Uygur population. METHODS:A total of 100 Uyghur patients with non-syndromic cleft lip and palate from Xinjiang were included as case group, and meanwhile, 60 children with upper respiratory infection were enrol ed as controls. Next-generation sequencing was used to detect coding region, 5’UTR and 500 bp before exon1 of IRF6 gene, and sequencing results were compared with the information on the genome database. RESULTS AND CONCLUSION:Eleven high-frequency SNPs were found:rs861019, rs7552506, rs2235377, rs2235371, rs2013162, rs7545538, rs7545542, rs12403006, rs846808, rs34743335, rs2235373. The frequencies of al eles of loci rs7545538 (C>G, P=0.007), rs7545542 (C>T, P=0.044) and rs2235373 (G>A, P=0.049) were significantly different in the case group and control group;the genotype distribution of rs7545538 showed significant difference between the case group and control group (P=0.037);the genotype distribution and the frequency of al eles of other loci had no significant difference between the case group and control group (P>0.05). Two blocks were identified in the 11 high-frequency SNPs. There were four common haploid types in Block 1:CCGGT>CCGAT>CACAT>TAGAC, and the most significant single type was CCGAT (P=0.032). There were three common haploid types in Block 2:TAC>ATG>TAG, and the most significant single types were TAC (P=0.009) and TAG (P=0.003). These findings indicate that IRF6 gene polymorphisms are probably associated with the occurrence of non-syndromic cleft lip and palate in Xinjiang Uygur population.

Objective: To investigate the current prevalence of excessive weight gain during pregnancy among pregnant and lying-in women in Xining City and to identify the influencing factors, so as to provide insights into reasonable weight control during pregnancy. Methods: The pregnant and lying-in women who were registered and delivered in the Red Cross Hospital of Qinghai Province, the Affiliated Hospital of Qinghai University and the First People's Hospital of Xining City from August 2021 to July 2022 were enrolled. Women's demographics, health status during pregnancy, diet, sleep and exercise were collected through prenatal testing data and questionnaires. Excessive weight gain during pregnancy was identified according to "Weight monitoring and evaluation during pregnancy of Chinese Women" (T/CNSS 009-2021), and the factors affecting excessive weight gain during pregnancy were identified using a multivariable logistic regression model. Results: A total of 902 questionnaires were allocated and 882 valid questionnaires were recovered, with an effective recovery rate of 97.78%. The pregnant and lying-in women had a mean age of (29.06±4.16) years, mean pregestational body mass index of (20.44±2.20) kg/m2 and mean gestational weight gain of (14.17±3.11) kg, and there were 447 primiparas (50.68%) and 360 women with excessive weight gain during pregnancy (40.82%). Multivariable logistic regression analysis identified an educational level of senior high school/technical secondary school (OR=1.400, 95%CI: 1.037-1.889), annual household income of >200 000 Yuan (OR=2.385, 95%CI: 1.924-2.956), a family history of diabetes (OR=1.475, 95%CI: 1.180-1.844), pregestational overweight/obesity (OR=4.079, 95%CI: 2.471-6.734), gestational hypertension (OR=2.061, 95%CI: 1.027-4.136), anxiety (OR=1.315, 95%CI: 1.139-1.518) and preference for sour foods (OR=1.715, 95%CI: 1.237-2.376) as risk factors for excessive weight gain during pregnancy, and early pregnancy reaction as a protective factor (OR=0.636, 95%CI: 0.546-0.742). Conclusions The prevalence of excessive weight gain during pregnancy was high in Xining City, and educational level, annual household income, family history of diabetes, pregestational body mass index, pregnant complications and dietary habits are factors affecting excessive weight gain during pregnancy.

Objective To evaluate the safety and efficacy of super mini-percutaneous nephrolithotomy (SMP) by ultrasound-guided renal access in pediatric with renal calculus.Methods From May to August 2015, 20 pediatric patients with upper tract stones underwent the SMP by ultrasound guidance.The patients aged 11-144 months , median age 31.5 months, The stone size ranged 0.8-2.5 cm, mean(1.48 ±0.59) cm.Among the 20 children, single pelvis stones were in 8, multiple stones in 10 and upper ureter stones in 2.All patients had no previous surgery treatment.The SMP system consists of a F6.0 -7.5 nephroscope and a modified F12-14 access sheath with suction-evacuation function.Nephrostomy tract dilation was performed up to F12-14 and lithotripsy procedure was performed by using pneumatic lithotripter.Nephrostomy tube or double J stent was placed only if clinically indicated.Results The stone size was 0.8-2.5 cm ,mean (1.48 ± 0.59)cm.Among the 20 children, there were 8 patients with single pelvis stone, 10 with multiple stones and 2 with upper ureter stones.All the patients were completed successfully without surgery conversion.Mean operative time ranged 6-40 minutes ,mean(17.6 ± 11.6) minutes.The stone free rate was 100％ after the evaluation in the postoperative day.The hemoglobin drop was 2-16 g/L,mean (7.6-± 4.1) g/L.No major complications occurred, neither patient required transfusion.The tubeless PCNL without double J stents and nephrostomy tubes placed were achieved in all patients.Only 14 patients had a ureter catheter placement for one day.The average hospital stay ranged 1-4 days, mean (2.4 ± 0.8) days.Conclusions SMP could be a safe and effective treatment for kidney stone up to 2.0 cm in pediatric cases with advantages of short recovery time, high stone free rate and no catheter placement.SMP could be the ideal procedure for children with upper urinary tract calculus.

Objective To systematically evaluate the effect of dexmedetomidine on postoperative cellular immune function in patients with malignant tumor.Methods The Medline,Cochrane Library,EMBASE,PubMed,OVID,Superstar Foreign Languages Database and Chinese Database,CNKI,Chinese Biomedical Literature CD-ROM Database and Wanfang Database were searched,and the publication was not limited by language or time.The randomized controlled trials involving the effect of dexmedetomidine on postoperative cellular immune function in patients with malignant tumor were collected.The evaluation indexes included the levels of whole blood T lymphocyte subsets CD3+,CD4+,CD8+ and natural killer cells and ratio of CD4+/CD8+.Meta-analysis was conducted using the Cochrane Collaboration's RevMan 5.2 software.Results Twelve prospective randomized controlled trials involving 692 patients were included in our meta-analysis.Compared with control group,the levels of CD3+,CD4+ and natural killer cells and ratio of CD4+/CD8+ were significantly increased at 1 day after operation (P＜0.01),and no significant change was found in the level of CD8+ in dexmedetomidine group (P＞0.05).Conclusion Dexmedetomidine can improve postoperative cellular immune function in patients with malignant tumors.