Objective To investigate the clinical diagnosis and treatment of pneumocystis carinii pneumonia(PCP) after kidney transplantation. Methods Eight patients who developed PCP between 90 days and 140 days following renal transplantation were enrolled in this study.All of them were male with a mean age of 28 years.The clinical manifestations,accessory examinations and treatment were retrospectively analyzed. Results All the 8 patients fully recovered.With early diagnosis and administration of compound sulfamethoxazole(SMZ),clinical symptoms of 6 patients were rapidly controlled within 5 to 7 days.Because of delayed diagnosis and treatment,the conditions of the other 2 cases were more severe and were controlled within 25 days and 46 days separately. Conclusions Application of percutaneous pneumocentesis biopsy can be useful for correct diagnosis in early stage of PCP and has high positive rate of the pathogen. Most PCP patients can be cured with early use of SMZ.Types and doses of immunosuppressor should be adjusted properly.

Objective To evaluate the efficacy and safety of two-dose basiliximab vs two-dose daclizumab for prevention of acute rejection in renal transplantation. Methods A total of 58 renal transplant recipients were randomized into 2 groups:basiliximab group ( n =30) and daclizumab group ( n =28).All the cases received the triple therapy of cyclosporine,mycophenolate mofetil and prednisolone (CsA+MMF+Pred).The 3 medications were administered as follows.For CsA,initial dose of 6 mg/kg per day was downtitrated to 4～5 mg/kg per day at 3 months,then to 3～4 mg/kg per day at 6 months.For MMF,initial dose of 0.5 g,3 times per day was downtitrated to 0.5 g,twice per day at 1 month.For Pred,initial dose of 30 mg per day was downtitrated to 20 mg per day at 3 weeks,then to 10～15 mg per day at 6 months.Basiliximab group received two-dose basiliximab (20 mg intravenous infusion) 2 hours before operation and 4 days after transplantation.Daclizumab group received two-dose daclizumab (50 mg) 1 day before operation and 14 days after transplantation.Postoperatively,acute rejection was monitored for 6 months,and adverse events and person/allograft survival were observed for 6～12 months.CD25 + cell count was tested using Beckman Coulter flow cytometer before operation and postoperatively once a week for 2 months. Results During 6-month follow-up, the number of acute rejection episodes were 5 in daclizumab group and none in basiliximab group ( P

0.05 ). In Zenapax and OKT3 groups, 1 and 2 allografts were removed as a result of rupture respectively. 4 cases were suffered from delayed graft function(DGF) in Zenapax group and 9 in OKT3 group respectively with the difference being significant ( P

Objective To observe the effect of pulse microwave combined with antibiotics on chronic prostatitis.Methods100 outpatients with chronic prostatitis were randomly divided into the trail group and control group with 50 cases in each group. The patients of the trail group were treated with pulse microwave combined with Ofloxoacin, and those of the control group were treated only with Ofloxoacin. The treatment course was 5 weeks. The evaluation with National Institutes of Health-Chronic Prostatitis Symptom Index (NIH-CPSI) and test of white blood cell (WBC) count in prostate secretion were performed before and after treatment for all patients of two groups.ResultsIn the trail group, the mean symptom degree score of NIH-CPSI was 9.3±2.6 and living quality score was 3.4±1.2, the total score was 12.4±4.1, and WBC count was 13.7±5.4/HP. In the control group, these indexes were 13.3±3.5, 4.5±1.2, 17.5±4.6 and 17.1±5.2 respectively. The NIH-CPSI score and WBC count of the trail group were lower than that of the control group ( P<0.05), and cure rate and total effective rate were higher than that of the control group ( P<0.05).ConclusionThe treatment of pulse microwave combined with Ofloxoacin for chronic prostatitis can improve the cure ratio and efficiency.

Objective To explore the value of phase-contrast magnetic resonance imaging (PC-MR) in diagnosis of secundum atrial septal defect (ASD) in pediatric patients. Methods Totally 42 patients (aged from 9 months to 15 years) with secundum ASD proved with tansthoric echocardiographic (TTE) were evaluated with PC-MRI. Images of the flow through ASD were obtained with PC-MRI. The distances of ASD rim to superior vena cava (SVC), inferior vena cava (IVC), atrioventricular valves (AVV) and right upper pulmonary vein (RUPV), as well as the entrances of the vena cava and right upper pulmonary vein (RUPV) were assessed. Results The sizes of ASD and distances of ASD rim to the adjacent structures (SVC, RVC, AVV and RUPV) at PC-MRI were well consistent with those of TTE in 42 patients (P<0.001). PC-MRI results in 26 patients correlated well with surgical results (P<0.001). With different velocity encoding, compared with surgical results, measurements of ASD's sizes were more accurate when setting velocity from 50 to 70 cm/s than 90 cm/s. Conclusion The shape of ASD can be virtually depicted with PC-MRI. PC-MRI can accurately assess the defect size, number, rim distances to adjacent structures, therefore providing a new method for depiction of congenital heart anomaly.

Objective To investigate the chronic inflammation causing effect of nanobacteria(NB) on the prostates of SD rats,and to provide experiment evidence for etiology of type Ⅲ prostatitis.Methods Forty adult,male rats were divided into model group(16 rats),treatment group(8 rats) and control group(16 rats) at random.The rats in model group and treatment group were reproduced by infusing NB suspension transurethrally,and the animals of control group received an infusion of normal saline.After 4 weeks,WBC,lecithine and pathological changes of prostates were observed in model group(8 rats) and control group(8 rats).Tetracycline(100 mg?kg-1?d-1) was administered to treatment group,and distilled water were given to other groups respectively for 4 weeks.Then all rats were sacrificed,and the same detections were done as before.NB from prostates of animal were re-isolated,cultured and identified.Results After 4 weeks,chronic inflammation were observed in model group.WBC became significantly higher and lecithine of prostates were lower in model group than in control group(P0.05).The positive cases of NB were 16 in model group,and none in control group.Conclusion Nanobacteria causes chronic inflammation in SD rat prostates.NB maybe the etiological agent of type Ⅲ prostatitis.Anti-NB treatment by tetracycline is effective for this inflammation.

Objective To report 56 cases of congenital heart disease with congenital single coronary artery and to evaluate the imaging diagnostic techniques. Methods All 56 patients with congenital single coronary artery underwent angiocardiography. Contrast enhancement magnetic resonance angiography (CE MRA) was performed in 4 cases. 48 cases were confirmed by operation. Results In these 56 cases, single left coronary artery was found in 44 cases and single right coronary artery was found in 12. Conclusion Congenital heart disease with congenital single coronary artery is not rare and correct diagnosis is very important for surgery.

Objective: To determine quercetin and kaempferol in stamen neulumbinis by HPLC. Methods : Shim-pack CLC-ODS column was used. Mobile phase consisted of methanol-0.025% phosphate. UV detector wavelength was set at 370nm. Results : The chromatographic peaks of quercetin and kaempferol and other components were completely separated. The average recovery was 96.8% and RSD was 1.97%. Conclusion : The experiment provided new markers and a reliable method for the quality control of Stamen Neulumbinis.

AIM: To determine adenosine content in Kudiezi Injection(Ixeris sonchifolia Hance). METHODS: HPLC was used with Shim-pack CLC-ODS(6.0 mm?150 mm,5 ?m). The mobile phase consisted of methanol-acelonitrile-0.025 mol?L -1 phosphoric acid (3∶4∶93). The flow rate was 1 mL?min -1 . The UV detection wavelength was at 260 nm. RESULTS: The adenosin content in Kudiezi Injection was 0.15/10 mL. The average recovery was 98.4%(n=5). CONCLUSION: HPLC can be used for the quality control of Kudiezi Injection Injection.