Pulmonary fibrosis can severely disrupt lung functions, but its etiology and pathogenesis remain unclear. Animal models of lung fibrosis play an important role in investigation of the mechanism by which pulmonary fibrosis develops. This review summarizes the characteristics, advantages, and disadvantages of widely used and newly established animal models of lung fibrosis.

OBJECTIVE:To determine the contents of ciprofloxacin and tinidazole in compound ciprofloxacin injection.MET_HODS:RP-HPLC method has been developed.The moble phase was 0.4mol/L citric acid-methanol-acetonitrile(2∶1∶0.8).The flow rate was 0.8ml/min.The detective wavelength was 277nm.RESULTS:The average recovery and relative stan_dard deviation were 99.7% and 0.9% for ciprofloxacin,99.6% and 0.7% for tinidazole.CONCLUSION:This method is rapid,simple,accurate and suitable for the quality control of this preparation.

Objective To establish a method of the dissolution test of Xinkeshu capsule and study the dissolution behavior of sodium Danshensu, protocatechuic aldehyde, salvianolic acid B and puerarin in Xinkeshu capsule.Methods Using the dissolution determination first law of Chinese Pharmacopoeia 2010 II edition, according to the dissolution test conditions in Japan′s“quality of medical drugs information set”, different Xinkeshu capsule were inspected in pH 1.2 solution,pH 4.0 acetate buffer, pH 6.8 phosphate buffer and water.The HPLC gradient elution method was adopted to determine the dissolution of sodium Danshensu,protocatechuic aldehyde,salvianolic acid B and puerarin in Xinkeshu capsule.ResuIts Dissolution of sodium Danshensu, protocatechuic aldehyde, puerarin and salvianolic acid B of Xinkeshu capsule sample from each manufacturer were in good condition, 45min dissolution were 80% and dissolution uniformity.ConcIusion The method is simple, accurate and reproducible for the dissolution determination of this capsule .

0.05).Both femur bone lengths in 3.2mm group were shorter than those in the 1.5 mm and 2.2 mm groups.No abnormalities of cartilage cells were observed,without obvious histological evidence of inflammation;only nonspecific foreign body reaction and macrophage infiltration were noticed.Conclusion: PDLLA rod itself does not cause any disturbance to the development of the epiphyseal plate.The growth disturbance of epiphyseal plate depends on the area of injured epiphyseal plate;a injured area over 7% of epiphyseal plate may cause the growth disturbance of epiphyseal plate.

Objective To compare the results of treatment with biodegradable ligaments and metallic fixation for total acromioclavicular joint dislocation. Methods Twenty eight patients with total acromioclavicular joint dislocation were treated with two different materials, 1) by Kirschner pin and steel wire or screw and steel wire tension band (MF group,16 cases), and 2) reconstructed by artificial coracoclavicular ligament and coracoacromial ligament (BF group,12 cases). There were 17 males and 11 females. All patients had acute dislocation. The time from injury to hospital admission varied from 0-8 days (average, 1.5 days). Results All patients were availble for an average duration of follow up of 39.5 months (range,8-70 months). The results were evaluated by radiographic representations and joint function according to Lazcano's standards. In BF group, 10 patients were assesed as good and 2 as fair. In MF group, 13 patients were assesed as good, 2 as fair and 1 as poor. There were no statistic differences between these two methods. Conclusion Biodegradable ligament fixation is believed to be a good simple method for the treatment of acromioclavicular joint dislocation without the necessity of a second operation for removal of implants.

Objective To assess whether 5-HTR1A C( - 1019) G and GNβ3 C825T gene polymorphisms are associated with post-stroke depression (PSD) and explore the genetic mechanism of the pathogenesis of post-stroke depression. Methods All 159 patients with first stroke were divided into the PSD group and the control group according to HAMD scores. Their genotypes were determined with polymerase chain reaction and allele-specific restriction enzyme analysis. Results The frequency of 5-HTR1A (-1019) GG genotype(8/53,15. 1% ), G allele (44/106,41.5%)and GNβ3 825T allele(68/106,64. 2% ) were significantly higher in the post-stroke depression group than in the controls (5/106,4.7% ;35/212, 16. 5%; 113/212, 53.3%; ×2 = 23.204, 23. 655, 3. 392, all P ＜ 0. 05 ). Combined genotype analysis showed that individuals with both 5-HTR1A ( - 1019) G and GNβ3 825T allele ( OR =4. 980,95% CI 2. 429-10. 210,P =0. 000) had a higher risk than those with 5-HTR1 A (-1019) G allele ( OR = 3. 589,95% CI 2. 113-6. 096, P = 0. 000) or GNβ3 825T allele ( OR = 0. 638,95% CI 0.395-1. 031 ,P =0. 042) only for post-stroke depression. Conclusion The 5-HTR1A C( - 1019)G and GNβ3 C825T polymorphisms are predisposing genes of post-stroke depression. Our data also suggest a significant interaction between the 5-HTR1A ( - 1019)G allele and GNβ3 825T allele in post-stroke depression.

Objective To explore the clinical significance of MDR1 gene expression inpatients with gastric cardia carcinoma.Methods Using reverse transcription-polymerase chain reaction(RT PCR), the genes expression of MDR1 and MRP, in 4 6 cases of gastric cardia cancer was determined.Results Positive expression of MDR1 and MRP gene were 6:3% and 50% respectively, which was muchhigher than that in tissue adjacent to 1ilo cancer site. In 9 cases of pre-operative chemotherapy , the levels ofMDR 1, and MRP gene expression were higher higher that without chemotherapy (P

SF-36 scale has been widely accepted because of its objectivity and scientificity. However, until now its application in TCM treatment for rheumatoid arthritis is not common. This article discussed the significance of SF-36 scale as the main content of life quality assessment in the efficacy evaluation system of TCM treatment for rheumatoid arthritis, which can provide certain references for the promotion of the application in this field.

Objective To observe the expression of FcRIIa and FcRIIb on the surface of several immune cells.Methods The expression of FcRIIa and FcRIIb on the surface of several immune cells were detected by RT-PCR and Western blot before and after IVIG incubation. The difference of Fc?RIIb expression on U937 and human peripheral blood mononuclear cells (PBM) after IVIG induction. was also analyze by FACS.Results Under normal conditions Fc?RIIa was mainly detected in macrophages/monocytes and B lymphocyte cells, while Fc?RIIb could not be detected. After IVIG administration, the expression of FcRIIb on the surface of U937 cells was quickly up-regulated (RT-PCR). FACS analysis revealed that the Fc?RIIb expression on U937 and human peripheral blood mononuclear cells (PBM) after IVIG induction was also increased. Conclusion The effect of immunoregulation of IVIG was partly achieved by up-regulation of the expression of FcRIIb on the surface of some immune cells.

MOS 36-Item Short-Form Health Survey has been widely recognized, but the application in the field of traditional Chinese medicine rheumatism was less. This article aimed to summarize the application of MOS 36-Item Short-Form Health Survey in Chinese medicine for rheumatism. This may provide certain reference for the future application.