Objective To evaluate clinical characteristics of acquired immunodeficiency syndrome (AIDS) patients infected with mycobacterium tuberculosis.Methods The clinical data of 440 AIDS patients infected with mycobacterium tuberculosis were collected and subjected to statistical analysis,including infectious type,sites,and situation.Results There were mainly multitopical or disseminated tuberculosis in 163 cases;the rate of extrapulmonary tuberculosis was 32.50%(143 cases);there were 48 cases of confidently extrapulmonay tuberculosis;there were 8 cases of adult primary pulmonary tuberculosis.Conclusion Acquired immunodeficiency syndrome patients infected with mycobacterium tuberculosis is the most opportunistic infection.There were multitopical or disseminated infection of mycobacterium tuberculosis in acquired immunodeficiency syndrome patients infected with mycobacterium tuberculosis.

This article evaluates the curative effect of intestinal sustenance of larygo-Carlinomactomee, the intestinal sustenance therapy has two stages. In the first stage (five days), patients are given nasal feeding liauid diet, takeing protein 44.7g, appropriate vitamin and ferralia every day, the total calorie is 1 631 large calories. In the second stage (eleven days), patients are given high caloric liguid diet, take in protein 65g, every day. The total caloric is 3 345 large calories. The result indicates: giving intestinal sustenance earlier has a good effect on laryngocarcinomactomee by improving the patients's immunity and accelerating the patients' early recover.

A catalytic spectrophotometric method for determination of trace molybdenum by flow injection analysis has been established, based on the catalytic oxidation of potassium iodide by hydrogen peroxide in the presence of molybedenum (VI) and the iodine produced is coloured with starch. The linear range and detection limit for molybdenum (VI) were 0 to 0.4 ppm and 3.2 ppb. The method has been successfully applied to determine molybdenum in the grain, soil and hair.

[Objective] To investigate the construction and inhibitory effects of artificial microRNA targeted on hepatitis B virus.[Methods] Four artificial microRNA sequences for HBV gene were designed with Invitrogen online design software (BLOCK-iTTM RNAi Designer),amiRNA eukaryotic expression plasmid were constructed and transfected into human hepatocellular carcinoma cell line HepG2.2.15.Hepatitis B virus DNA in supernatant was detected by quantitative fluorescence PCR.HBsAg and HBeAg detected by ELISA.[Results] All of the four artificial microRNA sequences could down regulate the level of HBV-DNA,HBsAg and HBeAg.miR3 had the most significant effect on inhibiting HBV-DNA replication.[Conclusion] Artificial microRNA sequences can effectively inhibit the replication of HBV in vitro.

Objective To analyze the DCE-MRI features and ADC value in triple-negative breast cancer and HER-2 overexpres-sion breast cancer,and to improve the j udgment of both characteristic in MRI.Methods To analyse the DCE-MRI imaging of TNBC and HER-2 overexpression breast cancer,measured the ADC value and compared the statistics.Results Compared with the shape, margin,the way of enhancement and ADC value of the TNBC and HER-2 overexpression breast cancer,the distinction has statisti-cally significant(P<0.05);there were no statistically significant difference among age,mass or nomass,categories of lesions and TIC,(P>0.05).Conclusion DCE-MRI features of the shape,margin,the way of enhancement and ADC values were useful for the diagnosis of TNBC and HER-2 overexpression breast cancer.

Objective To explore the level of knowledge,attitude and practice in preventing sudden deafness and the influencing factors among nursing students in Taiyuan City.Methods By random cluster sampling,1 187 nursing students from 6 universities in Taiyuan City were surveyed about the level of knowledge,attitude and practice and the influencing factors by questionnaires in November 2013.The results underwent analysis.Results Total scoring rate of knowledge,attitude and practice of preventing sudden deafness in the nursing students was 48.36％ (574/1 187),among which knowledge,attitude and practice was 28.30％ (336/1 187),62.68％ (744/1 187) and 54.34％ (645/1 187),respectively.Logistic regression analysis showed that whether taking health education or not,different parents' education degree and household capita monthly income had significantly different influence on level of knowledge,attitude and practice of preventing sudden deafness,P＜0.01.Significant correlation existed between knowledge and attitude,attitude and practice,r=0.467,0.149,P＜0.05.Conclusions The level of knowledge,attitude and practice of preventing sudden deafness of the nursing students is low and has muhifaceted influencing factors.Colleges and universities should strengthen the health propaganda,and community should step up intervention to the families of low income and low education degree,at the same time to improve the compliance of health behavior in order to play a role in prevention and treatment of sudden deafness among nursing students.

Objective To investigate the clinical features,treatment response and prognosis of patients with severe hemorrhagic fever with renal syndrome and complicated with acute anhemopoisis.Methods The clinical features of 18 patients with hemorrhagic fever with renal syndrome complicated with acute anhemopoisis were retrospectively analyzed.Resalts The clinical manifestations of the 18 adult patients(10 males and 8 females,age ranged from 14 to 55 years)with hemorrhagic fever with renal syndrome showed irregular fever,abrupt anemia and multiple sites hemorrhage at the severe stage(9-25 days after the onset).Peripheral leucocytes counts reduced to(2.3-3.2)×109/L,platelet to(23-31)×109/L,erythrocytes to(2.23-2.79)×1012/L,hemoglobulin to 52-67 g/L and reticulocyte to 0-0.002.Bone marrow test results showed that the hyperplasia of erythrocytes series decreased.The ratio of granulocyte to erythroeyte was significantly elevated,while granuloeyte and megalocaryocyte series decreased.The specific manifestations of acute anhemopoiesis were abrupt anemia,irregular fever and multiple sites hemorrhage.The major combined treatments were cortecosteroids and blood transfusion.These patients recovered at 26-60 days after the onset of the disease.Conclusions Hantaan virus could attack human bone marrow cells and induce cytopathogenic effect.In the course of hemorrhagic fever with renal syndrome,acute anhemopoisis could occur.The prognosis of these complicated cases is usually favorable.

Objective To study the effect of inhibitors of the multidrug transporters including P-glycoprotein (PGP)and multi-drug resistance-associated protein(MRP)on the regulation of concentration of oxcarbazepine in the extra-cellular fluid of the hippocampus after pilocarpine induced seizures in rats.To investigate whether oxcarbazepine are sub-strate for PGP and MRP and whether brain expressions of PGP and MRP are involved in muhidrug resistance mechanisms of refractory epilepsy.Methods The epileptic rats model were established by repeated peritoneal injection treatment with pi-locarpine.Thirty-two Wistar rats were divided into four groups:control group,pilocarpine epileptic model group,verapamil treated group and probenecid treated group.At 30,60,90,120 and 150 min following systemic injection of oxcarbazepine (80 mg/kg),dialysate was collected and the concentration of oxcarbazepine in the extracellular fluid of hippocampus was determined by microdialysis and high-performance liquid chromatography technique.Results After systemic injection of oxcarbazepine,the concentration of oxcarbazepine in extracellular fluid of the hippocampus during 90～150 min(1.26±0.09、0.93±0.10)were much higher in verapamil treated group than in pilocarpine epileptic model group(0.87±0.06、0.66±O.04)(P<0.05)and the concentration of oxcarbazepine in the hippocampus during 60～150 min(1.07 4±0.11、1.32±O.13、1.02±0.10、0.87±0.08)were higher in probenecid treated group than in pilocarpine epileptic model group (0.81±0.08、0.87±0.06、0.66.4±0.04、0.58±0.06)(P<0.05).Conclusions Oxcarbazepine are substrates for PGP and MRP and,penetration of oxcarbazepine through blood-brain barrier are restricted by PGP and MRP.Increased expres-sions of PGP and MRP in brain maybe involved in the mechanisms of multidrug resistance of refractory epilepsy.

Objective:To establish an HPLC method for the determination of levofloxacin in levofloxacin hydrochloride ophthalmic in situ gel. Methods:The chromatographic column was Agilent Zorbax C18(150 mm ×4.6 mm,5μm). The mobile phase consisted of hexane sulfonic acid sodium solution-methanol(72∶28). The flow rate was 1. 0 ml·min-1. The detection wavelength was 293 nm. The chromatographic column temperature was 40℃. The injection volume was 10 μl. Results:The calibration curve was linear within the range of 0. 040 3-0. 403 0 mg·ml-1(r=1. 000 0) for levofloxacin. The average recovery was 99. 8% (RSD=1. 08%,n=9). Conclusion:The method is accurate, simple and rapid, and suitable for the content determination of levofloxacin in levofloxacin hydro-chloride ophthalmic in situ gel.

Objective To evaluate the value of GeneXpert MTB/RIF assay in the clinical diagnosis of tuberculosis and tuberculosis associated rifampin resistance. Methods A total of 851 patients admitted to the Guangxi Longtan Hospital from September 22, 2015 to May 25, 2016 were enrolled. The smear microscopic examination, solid culture test, and GeneXpert MTB/RIF assay was performed on each patient. Drug sensitivity test of rifampicin using proportion method was done on patients infected with mycobacterium tuberculosis isolated by solid culture, and rifampicin resistance detection was performed on GeneXpert MTB/RIF positive patients. Bacteriological positive consistency test was performed on GeneXpert MTB/RIF and solid culture, and rifampicin resistance detection was compared bwtween GeneXpert MTB/RIF and drug sensitivity using proportion method. Culture and drug susceptibility testing were used as the gold standard, and the MTB sensitivity and specificity of smear microscopic examination, solid culture, and GeneXpert MTB/RIF assay was compared. Results Culture was used as the gold standard. Compared with culture, the sensitivity, specificity, positive predictive value, and negative predictive value of GeneXpert MTB/RIF was 95.48%(211/241), 94.91%(559/569), 87.55%(211/241), and 98.24%(559/569) respectively. The sensitivity of Xpert MTB/RIF for culture positive smear positive specimens and culture positive smear negative specimens was 98.78% (162/164) and 85.96%(49/57) respectively. Drug susceptibility testing was used as the gold standard. Compared with drug susceptibility testing, the sensitivity, specificity, positive predictive value, and negative predictive value of rifampin resistance measured by GeneXpert MTB/RIF was 98.70%(152/154), 92.98%(53/57), 97.44%(152/156) and 96.36%(53/55) respectively. The consistency rate of GeneXpert MTB/RIF and solid culture was 93.83% (Kappa = 0.847), and that of GeneXpert MTB/RIF rifampin resistance detection and proportion method for drug sensitivity was 96.38% (Kappa = 0.904). Conclusions The GeneXpert MTB/RIF detection is easily operated with high sensitivity and specificity compared with smear microscopic examination and solid culture, and it also possesses good consistency with solid culture and traditional drug sensitivity. Moreover, it could also be applied to diagnose the resistance to rifampin, which is very promising in application.