AIM:To investigate whether the protective effect of adiponectin on glucose and lipid metabolism is achieved through down-regulating major histocompatibility complex classⅡ( MHCⅡ) in the adipose tissue.METHODS:Adiponectin knockout ( KO) mice and C57BL/6 ( WT) mice were fed with high-fat diet and standard diet for 24 weeks, re-spectively.The body weight, fasting blood glucose (FBG), fasting insulin (FINS), homeostasis model assessment of insu-lin resistance (HOMA-IR), triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol ( HDL-C) , hepatic histology, and classⅡtrans-activator ( CIITA) , histocompatibility 2 class II antigen E beta (H2-Eb1) and cluster of differentiation 74 (CD74) mRNA and MHC II protein levels in adipose tissue were measured at sacrifice.siRNA targeting MHC II and overexpression vector was used in 3T3-L1 cells to explore the effect of adiponectin on the protein level of MHCⅡ.RESULTS:The levels of body weight, FBG, FINS, HOMA-IR, TC, TG, LDL-C, hepatic steatosis, CIITA, H2-Eb1 and CD74 mRNA expression, and MHCⅡ protein expression in the KO mice were higher than those in the WT mice that fed with high-fat diet or standard diet.In 3T3-L1 cells, inhibition of adiponectin reversed MHC II protein level induced by specific siRNA.The expression of MHC II in adipocytes decreased after adiponectin was overexpressed.CONCLUSION: Adiponectin improves glucose and lipid metabolism through sup-pressing the expression of MHCⅡin the adipose tissue.

Objective To explore the curative effects of artificial skin(hydrocolloid dressing) and erythromycin ointment in the treatment of neonatal skin injury, and to provide theoretical evidences for the promotion of clinically rapid recov-ery of neonatal skin injury and the improvement of curative effects. Methods A total of 160 patients with neonatal skin injury who were treated in our hospital from March 2014 to March 2015 were selected. They were randomly and aver-agely assigned to two groups, research group and control group. Infant patients in the control group were given the treatment of erythromycin ointment, and infant patients in the research group were given the treatment of artificial skin (hydrophilic dressing). Wound healing time, average frequency of dressing change, incidence of complications, non-cure rate and effective rate of treatment were compared between the two groups of infant patients. Results In infant patients of the research group, wound healing time was (4.47±0.76) d, average frequency of dressing change was (3.18±0.52) times, incidence of complications was 5.00%, non-cure rate was 3.75% and effective rate of treatment was 95.00%. All in-dices in the research group were significantly better than those in the control group, and the differences were statisti-cally significant (P<0.05). Conclusion Artificial skin (hydrophilic dressing) in the treatment of neonatal skin injury is able to promote the wound healing rapidly, can reduce the frequency of dressing change and improve the effective rate of treatment.