Objective:To learn about the normativity and differences among regions and institutions of the fee of counseling and psychotherapy in China.Methods:The fee standards of 142 mental health service institutions released on their websites were collected through intemet query,the overall situation and the aspects of regions and instimtions of the fee of counseling and psychotherapy were analyzed and compared.Results:Ninety-one institutions set fee standards which had great disparity according to either the practitioner's professional ritles or their qualifications.Private institutions had a significant higher level of both the average minimum and maximum fees of interview counseling than public hospitals (Ps ＜0.00).The differences in the maximum fees of interview counseling among different regions were found to be statistically significant (P ＜ 0.001),the maximum fees of interview counseling in eastern areas were significantly higher than that in central and westem areas,the maximum fees of interview counseling in provincial cities were significantly higher than that in non-provincial cities (P ＜ 0.05).Conclusion:The currein fee of counseling and psychotherapy lacks overall regulations.The fee of counseling and psychotherapy should take an overall account of the nature of institutions,the regions,the qualifications of practitioners and the specific conditions of the clients so as to set proper fee standards,thereby promoting the development of mental health services.

Objective To assess the clinical application value of magnetic susceptibility-weighted imaging in cerebral vascular malformations. Methods Thirty-six patients with cerebral vascular malformations were enrolled in the study. All the patients received examination of T1WI, T2WI and SWI. SWI sequence was compared with T1WI, T2WI sequence in detecting and diagnosing cerebral vascular malformations. Results Cerebral vascular malformations were low signal in SWI sequence. Cavernous angioma, developmental venous anomalies and AVM had been found in 73,8,10 cases, respectively. The bounder of lesions was clearer, more multiple small lesions, more hair-thin pulp veins and draining veins were detected in SWI sequence. Conclusion SWI sequence has more sensitivity than T1WI and T2WI sequence in detecting the cerebral-vascular malformations. SWI sequence may become routine examination se- quence in diagnosis cerebral-vascular malformations.

Objective:In order to understand the professionals' ethical ideas and provide references for ethics education, and try to set up Chinese ethical code, the ethical beliefs of Chinese counselors and psychotherapists were investigated. Methods:145 Chinese counselors and therapists were asked to fill up an ethical questionnaire which was translated from the survey developed by Gibson and Pope in 1993 in the United States. The results of the survey were analyzed and compared with that of American professionals. Results: Chinese participants showed strong ethical beliefs in general, but they need more training in the aspect of informed consent. There were apparent culture differences on some aspects between two samples. Some significant gender and age differences could also be found. The authors believe that ethical training should be considered as an important way to enhance the professionals' awareness on ethical codes.

Objective: To study the relationship between mental health and achievement motivation of undergraduates. Method: 532 undergraduates completed SCL-90 and Academic Achievement Motive Scale. Results:17.29% subjects had mental problems. Undergraduates had higher score in self-oriented motive than society-oriented motive, while the score of SCL-90 correlated positively with score in society-oriented motive. In students with poor academic achievement, the score of SCL-90 negatively correlated with score in self-oriented motive. Conclusion:Society-oriented motive had negative influence on mental health of undergraduates and in those with poor academic achievement, poor mental health positively correlated with lower self-oriented motive.

Objective:To evaluate the in vitro and in vivo immunostimulatory activity and the safety of ethanol extract of wild Artemisia rupestris L. (EEWAR). Methods:Bone marrow dendritic cells (BMDCs) from C57BL/6 mice were treated with different concentrations of EEWAR in vitro and the expression of CD40 and CD80 on BMDCs was detected by flow cytometry. ICR mice were subcutaneously immunized with different concentrations of EEWAR in combination with ovalbumin (OVA) or OVA alone. Aluminum adjuvant was used as the positive control. OVA-specific IgG antibodies in mouse serum samples were measured by ELISA following immunization. T cell proliferation in spleen tissues was detected by MTT method. Acute toxicity test was conducted in ICR mice to analyze the safety of EEWAR. Results:In vitro experiment showed that EEWAR at the concentrations of 10-20 μg/ml increased the expression of CD40 and CD80 on BMDCs ( P<0.05), and had no significant effect on the morphology of BMDCs; EEWAR at the concentrations of 100-200 μg/ml significantly promoted the expression of CD40 and CD80 on BMDCs ( P<0.01), but had a certain influence on the morphology of BMDCs. In vivo experiment showed that EEWAR enhanced the production of IgG, IgG 1 and IgG 2a antibodies against OVA and the proliferation of splenocytes ( P<0.05). In the acute toxicity test, EEWAR at the concentrations of 50-5 000 μg/ml had no side effects on mouse body weight and was relatively safe. Conclusions:EEWAR could promote the maturation of DCs and enhance the humoral and cellular immune responses when used as an adjuvant to OVA. It was safe in a certain dose range. This study provided reference for further research on EEWAR as a new-generation adjuvant.

Objective To investigate the levels of serum chemerin in the acute myocardial infarction (AMI) patients and explore the clinical significance.Methods A total of 76 AMI patients from January 2013 to December 2013 were inrolled and 30 healthy patients were included as normal control(NC) group.Chemerin, tumor necrosis factor α(TNF-α), adiponectin were asessed by enzyme linked immunosorbent assay(ELlSA).The clinical data of high sensitivity C reactive protein (hs-CRP), body mass index (BMI), blood lipids, underlying diseases et al were collected.Results Serum levels of chemerin in AMI patients was higher than the NC group significantly((12.97±4.17) μg/L vs.(30.96± 17.16) μg/L, t =5.642, P＜0.001).The Serum levels of Chemerin in single vessel disease group, two vessel disease group and triple vessel disease group were (22.25 ± ±6.93) μg/L, (28.57 ± 15.92) μg/L, (37.95 ± 16.52) μg/L respectively and was linearly increasing relationship with increasing AMI severity (P＜0.001, F =22.84, r2 =0.397).Serum levels of Chemerin positively correlated with TNF-α, hs-CRP level(r2=0.347,0.455 ,P＜0.001) and negative correlated with adiponectin(r2 =0.396,P＜0.001).Logistic regression analysis showed that chemerin was an independent risk factor for AMI (OR =4.822,95％ CI 4.422-7.141, P =0.032).Conclusion The results showed that serum chemerin levels were significantly elevated in AMI patients,and chemerin may be involved in the development process of AMI.

Objective To study the influence of seminal plasma reactive oxygen species(ROS),cell factors and sperm quality in infertile male with Uu infection and explore its action mechanism in male infertility.Methods Chose 83 cases of male in-fertility with Uu infection as the experimental group (Uu+ infertility group),30 cases of male infertility without Uu infec-tion (Uu- infertility group)and 30 normal men with children as a control (Normal fertility group).Respectively,determi-nate the levels of seminal plasma malondialdehyde (MDA),superoxide dismutase (SOD),IL-6,IL-10,IL-18 and TNF-α,and analyzed its correlation.Results In Uu+ infertility group,the levels of MDA (19.56±5.22 nmol/ml),IL-6 (58.31±8.94 pg/ml),IL-18 (38.16±17.02 pg/ml)and TNF-α(42.68±11.18 pg/ml)were obviously higher than those in the other two groups (t=4.35~20.43,P value<0.001),and the level of IL-10 (8.62±2.98 pg/ml)and SOD (95.36±20.03 μmol/L) was lower than those in the other two groups (t=3.67~23.74,P value<0.001).Correlation analysis found that MDA of Uu+ infertility group was positively correlated with TNF-α,IL-18 (r=0.61,0.55,P value<0.001),and negatively correla-ted with SOD and IL-10 (r=-0.55,-0.53,P value<0.001).Conclusion The results suggested that Uu infection caused the level of reactive oxidative increasing,cytokines counterbalance disorder and affected sperm quality.So it is of great signif-icance for the treatment to test the levels of ROS and cytokines in patients with male sterility.

Objective:Present study was aimed to analyze the immunogenicity of recombinant Lugurus zona pellucida-3 protein (r-LZP3) expressed in E.coli,and to evaluate the efficacy of its antiserum to affect in vitro mouse sperm-egg binding.Methods:Female mouse were immunized using LZP3 DNA vaccine for priming,and boosted with r-LZP3 protein.The antibody response level of LZP3 antisera was determined by ELISA.The immunoreactivity and specificity of the anti-LZP3 antisera were tested by immunoblot, indirect immunofluorescence and immunohistochemistry using native mouse ZP.A sperm-zone pellucida binding assay was used to evaluate the efficacy of anti-LZP3 antisera to inhibit mouse sperm-egg binding in vitro.Results: The result of ELISA showed that LZP3 DNA vaccine was able to induce higher antibody titers in mouse.Antiserum could specifically recognize or bind to r-LZP3 protein expressed in E.coli and native mouse ZP in vitro.The LZP3 antisera and r-LZP3 protein also inhibited sperm-egg binding in vitro.Condusion:These results show that r-LZP3 protein is of strong immunogenicity.

Objective To investigate the efficacy of using crude polysaccharides extracted from cultivated Artemisia rupestris L. ( CARCP) in Xinjiang as an immunologic adjuvant for ovalbumin ( OVA) . Methods The mice were subcutaneously immunized twice with OVA vaccine formulated with CARCP. ELISA assay was performed to measure the levels of IgG, IgG1 and IgG2a antibodies. Flow cytometry analy-sis was performed to detect the percentages of different T lymphocyte subsets ( CD3+CD4+ and CD3+CD8+T cells, CD4+CD44+ and CD8+CD44+T cells) in splenocytes as well as the expression of intracellular cyto-kines ( CD4+IFN-γ, CD8+IFN-γ) and CD4+CD25+Foxp3+Treg cells. Results The levels of IgG, IgG1 and IgG2a antibodies in serum, the percentages of CD3+CD4+, CD3+CD8+, CD4+CD44+ and CD8+CD44+T cells as well as the ratio of CD4+/CD8+T lymphocytes increased significantly in mice immunized with OVA in combination with CARCP (P<0. 05). Moreover, CARCP enhanced the expression of CD4+IFN-γand CD8+IFN-γ( P<0. 05 ) , but inhibited the expression of CD 4+CD 2 5+Foxp 3+Treg cells ( P<0. 05 ) . Conclusion As an adjuvant for OVA, CARCP enhanced the humoral and cellular immune responses, especially the T cell immune responses.

Objective To evaluate MRI characteristics of solid papillary carcinomas (SPCs) in situ of the breast.Methods A retrospective study included 5 patients with pathologically confirmed SPC in situ was performed.MRI data before operation including conventional MRI,dynamic contrast enhanced MRI (DCE-MRI) and DWI were analyzed.Results All the lesions showed iso/hypointensity on T1 FSPGR sequence,iso/hyperintensity on FSE T2WI sequence and STIR sequence.Mass enhancements were observed for all lesions with oval or irregular shapes on DCE-MRI.The margin of lesions were circumscribed,and internal enhancements were homogeneous or heterogeneous.Time intensity curve appeared a rapid increase in initial contrast phases and platform or outflow types in delayed phases.All the lesions on DWI showed slightly hyperintensity with the ADC value range from 1.34 × 10-3 mm2/s to 1.96)× 10-3 mm2/s.Conclusion MRI manifestations of SPC are characteristics,which may provide valuable information to distinguish SPC in situ from other invasive breast carcinomas.