Objective To explore the changes of the levels of IL-8,LTB4 and its function in invasion in patients with chronic obstructive pulmonary disease(COPD).Methods The concentrations of IL-8 and LTB4 in serum and sputum were determined with enzyme linked immunosorbent assay(ELISA)in 30 cases of patients with COPD in acute aggravating stage and paracmasis and in 20 cases healthy adults.Blood routines were detected in all obstructs.Results The concentrations of IL-8 in serum and sputum in acute aggravating stage were(181.41±18.49)ng/L and(74.03±20.64)ng/L,significantly higher than those in paracmasis and the reference group(152.33±17.48)ng/L and(42.30±14.99)ng/L,P＜0.05).The concentrations of IL-8 in serum and sputum in paracmasis were significantly higher than those in reference group[(52.75±13.79)pg/ml and(17.15±5.36)ng/L,P＜0.05].The concentrations of LTB4 in sputum in acute aggravating stage were(2300.91±496.01)ng/L,significantly higher than those in paracmasis and the reference group[(1884.14±651.37)ng/L,P＜0.05].LTB4 level in acute aggravating is not significantly different from those in paracmasis and the reference group[(1941.30±301.62)ng/L and(1880.77±302.11)ng/L,P＞0.05).Conclusion Both IL-8 and LTB4 play important roles in the coupe of inflammatory reaction of air passage of COPD.

OBJECTIVE:To study the anti-inflammation,analgesia and microcirculation improvement actions of Runingshuang.METHODS:The animals were randomly divided into control group,Sanjierupi glue group and lose dose and high dose Runingshuang groups.The curative effects of the externally applied Runingshuang on dimethylbenzene-induced earlap turgescence and the agar-induced chronic granulomatous inflammation were monitored.And the analgesic effect of the intragastrically administered or externally applied Runingshuang and its antagonistic action in microcirculation disturbance caused by pituitrin were determined by glacial acetic acid writhing method and hot plate method.RESULTS:The inhibitory rate of high and low dosage of Runingshuang on the earlap turgescence caused by dimethylbenzene were 46.2%and 43.1%,respectively,which were significantly different as compared with the control group(P

Objective The expression of Ang-2 and HSP90α in serum of patients with non-small cell lung cancer and their correlation with TNM staging were analyzed.Methods From January 2015 to June 2016,40 patients with elderly non-small cell lung cancer and 40 healthy persons were selected as the study subjects.The serum levels of Ang-2 and HSP90 alpha in the subjects were examined and their correlation with the TNM stage of the patients was analyzed.Results Observation of Ang-2 and HSP90α levels in the serum of the patients group were significantly higher than the control group,and the difference was statistical significance(P<0.05);Serum Ang-2 and HSP90α levels TNM Ⅰ-Ⅱ patients were significantly lower than that of stage Ⅲ and Ⅳ,the difference was statistical significance(P<0.05);Serum Ang-2 and HSP90α levels in TNM Ⅲ patients was significantly lower than those in stage Ⅲ and Ⅳ,the difference was statistical significance(P<0.05);TNM staging was positively correlated with the levels of HSP90α in serum,and statistical significance(r=0.425,P<0.05),TNM stage had a remarkably positive correlation with the level of Ang in serum(r=0.525,P<0.05).Conclusion The levels of Ang-2 and HSP90α were significantly higher in the serum of elderly patients with non-small cell lung cancer,and the expression level was positively correlated with TNM stage.

Objective:To observe the general status, the effect of resisting to the aggression of FBL-3 cells and protective immunity of the mice by using leukemia vaccine during the immune reconstitution.Methods:The immune deficiency C57BL/6 mice induced by total body irradiation were reconstituted immunity with homologous splenic lympocytes.The mice were immunized by injecting FBL-3 cells subcutaneously,and then attacked by using FBL-3 cells 7days thereafter.The activities of mice,the percentage of carcinoma formation and the growth status of tumor were observed.The amount of IFN-? in peripheral blood was determined by ELISA to evaluate the magnitude of cell immunity.The condition of cell infiltration in tumor central region and its periphery region was explored by patologic-section.Results:Immune reconstitution could obviously elevate protective immunity on the basis of using vaccine,and the amount of IFN-? was obviously higher.The FBL-3 tumors inoculated thereafter grwe slowly,and significant infiltration of mononuclear cells peri-tumor as well as necrosis of tumor cells were observed.Also,the life time of the mice was prolonged.Conclusion:Immune reconstitution and vaccine can significantly elevate protective immunity

Objective To study the curative effects and adverse effects of the thalidomide combined with COMP chemotherapy in treating multiple myeloma(MM).Methods 42 patients were initially diagnosed as MM and 27 patients were refractory and relapsed MM.The small dose of thalidomide combined with COMP management and COMP management alone were used.The effective rate and adverse effects were analyzed.Changes of M-protein in serum,percentage of plasma cells in bone marrow and the level of hemoglobin were also analyzed in both pre-treatment and post-treatment periods.Results In 42 patients who were initially diagnosed as MM,the effective rate was 40.9% for 22 patients treated by chemotherapy alone and 70.0% for 20 patients treated by the thalidomide combined with chemotherapy.Statistic difference was observed between those two group.As to the 27 patients who were refractory and relapsed MM,the effective rate was 42.9% for 13 patients treated by chemotherapy alone and 84.6% for 14 patients treated by the thalidomide combined with chemotherapy.Statistic significance was present between them.Adverse effects were less and tolerated.Conclusion Treatment of small dose of thalidomide combined with COMP chemotherapy could significantly improve the effective rate with less adverse effects.

Objective To investigate the enhancement characteristics of the mass of liver and pancreas on Mn-DPDP enhanced MRI. Methods Mn-DPDP enhanced MRI were carried out in 25 cases, of which 15 cases had examined by Gd-DTPA-enhanced MRI. Conventional plain with SE T 1WI、FSE T 2WI were done in all patient. SE T 1WI and FSPGR T 1WI were performed during 40 minutes～4 hours after the administration of Mn-DPDP, with a dose of 0.5 ml/kg.Parts of the patients were repeat examined 24 hours after the administration of Mn-DPDP. Results Among 10 cases of hepatocelluler carcinoma, slightly heterogeneous enhancement were found in 8 cases.hemi-ring enhancement were found in 2 cases. 2 cases of liver cirrhosis had apparently nodular homogenous enhancement and their signal intensity were higher than normal liver tissue. No enhancement were found in 2 cases of hemangioma, 2 cases of liver metastases and 1 case of hepatic cyst. Also no enhancement were found in 8 cases of pancreatic carcinoma, but the tissue of pancreas around the tumor were enhanced remarkably. Conclusion The Mn- DPDP can show remarkably enhancement of normal liver and pancreas.The medicine's peak value keeps on time grows, can provide plentiful scanning time windows.

BACKGROUND: Coronary artery disease (CAD) risk in type Ⅱ diabetics is one of the hot topics in clinical research.OBJECTIVE: By using electron-beam tomography(EBT) technology, heart scans were performed to investigate coronary artery lesion in type Ⅱ diabetics and their CAD risks were evaluated as well.DESIGN: Comparative study based on type Ⅱ diabetics complicated with coronary artery lesion.SETTING: Gerontology department in a hospital affiliated to a military medical university of Chinese PLA.PARTICIPANTS: From January 2002 to June 2004, 93 inpatients between the ages of 36 and 78 in the Gerontology Department of Xijing Hospital, an affiliation to the Fourth Military Medical University of Chinese PLA, were selected(male 80, female 13) . Totally 25 of all the patients proved normal by physical examinations were set as normal control(male 22, female 3) group; other inpatients were divided into the abnormal blood glucose group and the coronary heart disease (CHD) group respectively according to their examination results. Of the 57 patients in the abnormal blood glucose group,49 were male and 8 were female. This group was divided into two subgroups:the impaired glucose tolerance group(IGT) containing 29 patients and the diabetes group containing 28 patients. In this group, 44 patients have had heart symptom of different extent. Of the 11 patients in the CHD group, 9 were male and 2 were female. Inclusion criteria: patients with type Ⅱ diabetes were defined by American Diabetes Association in 1979; patients with CHD were defined in the report of the Joint International Society and Federation of Cardiology/World Health Organization Task Force on the standardization of clinical nomenclature and criteria for diagnosis of ischemic heart disease in 1979, and they were all confirmed by coronary angiography. Exclusion criteeases.METHODS: Both plain EBT heart scanning and contrast-enhanced heart scanning were performed on the 93 patients to evaluate the occurrence of coronary artery calcification(CAC), the number of stenosed coronary arterie,and the number of soft plaques. Additionally, other examination results including blood glucose, blood HbA1c and blood lipid were also collected for comparison between the conditions of coronary artery and assessment of the CHD risk among groups.MAIN OUTCOME MEASUREMENTS: In all the groups, the occurrence of CAC, the number of stenosed coronary arteries and the number of plaques were compared, and the CHD risk was analyzed.RESULTS: The occurrence of coronary artery calcification was significantly increased in all the study groups as compared with the control group(x2 = 18.88, P < 0. 01 ). Increasing trend was also observed in the study groups when calculating the number of branches with coronary stenosis and plaques ( F = 10. 758, P < 0. 01; F = 9. 991, P < 0.01 ). CHD risk in diabetes was estimated to be 7. 514 (95% confidence interval, 1. 885 -63. 778).CONCLUSION: Coronary artery lesion can be easily observed in type Ⅱ diabetics,and high CHD risk is predicted in this population. Early diagnosis of CAD and early rehabilitation intervention in grade 2 are extremely important in improving the quality of life and reducing the disability ratio of type Ⅱ diabetics.Huang C, Gao JY, Wong XM, Li R, Yang AL, Zheng MW, Xue FB. Risk analysis of coronary artery disease in type Ⅱ diabetics.

OBJECTIVE: To preliminarily determine the gene mutation frequency and the hotspots in Henan province, we analysed the deafness-related gene mutation in patients with non-syndromic hearing loss (NSHL). METHOD: Genomic DNA samples of 100 patients with NSHL in Henan province were extracted from peripheral blood after clinical history inquiry and clinical examination, Four common deafness genes GJB2, SLC26A4, mitochondrial 12SrRNA, and GJB3 were detected by Sanger sequencing method,and then data analysis were conducted. RESULT: Among 100 patients with NSHL. the gene mutation frequency was 44%. In these patients, 29 cases had GJB2 mutations, 13 cases had SLC26A4 gene mutations, and 3 cases had mitochondrial 12SrRNA mutations. CONCLUSION Among the patients with NSHL in Henan province, the most frequent mutation causing hereditary deafness was mutation in GJB2, followed by SLC26A4,and it will provide a theoretical basis to determine the etiology of deafness in Henan Province.
China ; Connexin 26 ; Connexins ; genetics ; DNA Mutational Analysis ; DNA, Mitochondrial ; genetics ; Deafness ; genetics ; Gene Frequency ; Humans ; Membrane Transport Proteins ; genetics ; Mutation ; RNA, Ribosomal ; genetics ; Sulfate Transporters

Objective To observe the efficacy of HAG regimen in remission inductive treatment for elderly patients with acute myeloid leukemia (AML) and myelodysplastic syndrome-refractory anemia with excess blasts (MDS-RAEB). Methods The clinical features of 21 cases with AML and 9 cases with MDSRAEB (≥60 year old) treated with HA-G regimen in remission induction were retrospectively analyzed,including the complete remission (CR) rate, efficiency rate as well as their toxicities. Results In 21 elderly patients with AML treated with HAG regimen, the efficiency rate was 66.7 % (14/21), CR rate 47.6 % (10/21).In 9 elderly patients with MDS-RAEB, the CR rate was 55.6 % (5/9). The main toxicity of HA-G regimen was infections secondary to hematopoiesis suppression after chemotherapy. All patients were well tolerated to adjusted regimen. Conclusion The HA-G regimen is much effective in remission induction for elderly patients with AML and MDS-RAEB.

Objective To assess the value of real-time myocardial contrast echocardiography (RTMCE) and intermittent triggered myocardial contrast echocardiography (ITMCE) in the detection of myocardial no-reflow phenomenon after reperfusion in acute myocardial infarction on mini-swine models. Methods Thirty close-chest mini-swines were used to create acute myocardial infarction and reperfusion model through interventional method. RTMCE and ITMCE were performed at baseline, 2 h after occlusion of left anterior descending coronary artery and 3 h after reperfusion. The myocardial perfusion defects after occlusion was measured as risk area (RA) and that after reperfusion was measured as no-reflow area (NRA). NRA/RA was calculated and compared with pathological findings. Results The whole study protocol was successfully performed in 27 mini-swines. NRA/RA obtained from RTMCE, ITMCE and pathological staining was (47.94±21.29)%, (38.20±21.04)% and (30.07±14.62)% , respectively. NRA/RA had no significant difference by ITMCE and pathological staining (P=0.124), RTMCE and ITMCE (P=0.071). The correlation coefficient of RTMCE and staining was 0.700 (P<0.001), ITMCE and staining was 0.765 (P<0.001), RTMCE and ITMCE was 0.897 (P<0.001). The sensitivity, specificity and accuracy in the detection of myocardial no-reflow was 100%, 58.33% and 79.17% for RTMCE, 91.67%, 73.33% and 81.48% for ITMCE. Conclusion Both RTMCE and ITMCE could be used as noninvasive methods to reveal the myocardial perfusion and quantitatively detect myocardial no-reflow after reperfusion therapy.