Objective To research the mechanism of treating cyclomastopathy with Runingshuang by measuring the blood rheology and mammary microcirculation. Methods 70 female Wistar rats of SPF were randomly divided into seven groups:the blank control group, the pathologic model group, the comparative group of tamoxifen ointment, the comparative group of Sanjierupi Glue, three remedial groups with large, middle and small dosage of Runingshuang. Pathologic model of mammary hyperplasia was made by injecting estrogen and progesterone. Except the former two groups, each group was treated with different drugs. The blood rheology and mammary microcirculation were measured after 30 days. Results Compared with the blank control group, blood viscosity, plasma viscosity, packed cell volume and the fibrinogen of the pathologic model group increased evidently (P

OBJECTIVE:To study the anti-inflammation,analgesia and microcirculation improvement actions of Runingshuang.METHODS:The animals were randomly divided into control group,Sanjierupi glue group and lose dose and high dose Runingshuang groups.The curative effects of the externally applied Runingshuang on dimethylbenzene-induced earlap turgescence and the agar-induced chronic granulomatous inflammation were monitored.And the analgesic effect of the intragastrically administered or externally applied Runingshuang and its antagonistic action in microcirculation disturbance caused by pituitrin were determined by glacial acetic acid writhing method and hot plate method.RESULTS:The inhibitory rate of high and low dosage of Runingshuang on the earlap turgescence caused by dimethylbenzene were 46.2%and 43.1%,respectively,which were significantly different as compared with the control group(P

Objective To study the feasibility of noninvasive detection of unstable plaques with 18F-Fluorodeoxyglucose (18F-FDG) PET/CT imaging. Methods Atherosclerotic plaques were induced in male New Zealand white rabbits. Animals were injected with FDG labeled with 18F,then examined with PET/CT. Aorta was explanted for photography with digital camera,and 18F-FDG uptake analysis. Thirty unstable plaques and 30 stable plaques were choosed so as to compare the quantitativly 18F-FDG uptake.The number of macrophages and smooth muscle cells was detected by immunohistochemical technique. Results Experimental group showed inconsistent uptake of 18F-FDG in the abdominal aorta. The results were confirmed in the ex vivo digital photo of the explanted aorta. The target to non target ratio(T/NT) and macrophages of unstable plaques were higher than stable plaques(P

Objective To study the feasibility of noninvasive detection of unstable plaques with ~(18)F-Fluorodeoxyglu-cose (~(18)F-FDG) PET/CT imaging. Methods Atherosclerotic plaques were induced in male New Zealand white rabbits. Animals were injected with FDG labeled with ~(18)F, then examined with PET/CT. Aorta was explanted for photography with digital camera, and ~(18)F-FDG uptake analysis. Thirty unstable plaques and 30 stable plaques were choosed so as to compare the quantitativly ~(18)F-FDG uptake. The number of macrophages and smooth muscle cells was detected by immunohistochemical technique. Results Experimental group showed inconsistent uptake of ~(18)F-FDG in the abdominal aorta. The results were confirmed in the ex vivo digital photo of the explanted aorta. The target to non target ratio (T/NT) and macrophages of unstable plaques were higher than stable plaques (P<0.01) , but smooth muscle cells obviously reduced (P <0. 01). Correlation analysis showed that there was a positive correlation between T/NT and macrophage content (r=0. 815,P<0. 01), and a negative correlation between T/NT and SMC content(r=-0. 684,P <0. 01). Conclusion ~(18)F-FDG PET/CT can constitute an attractive imaging method for the noninvasive detection of experimental unstable plaques.

Objective To study the relation between mammary hyperplasia and expression of VEGF,bFGF. Methods After the breast mass undergoing core needle biopsy, the pathological type and expression of VEGF and bFGF were observed in 140 cases of breast mass. Results Of the 140 cases, general ~hyperplasia was found in 92 cases( 65.7%), atypical hyperplasia in 48 cases(34.3%) . The expression of VEGF and bFGF were increased with the increase of pathological degree of breast hyperplasia (P

Objective To analyze the expression of the placenta Grb10 from women conceived by transferred thawed blastocyst,and to evaluate the security of blastocysts vitrification.Methods A cross-sectional study was performed in the Department of Obstetrics and Gynecology of Fujian Provincial Maternity and Children's Hospital from January 2012 to May 2014,50 women conceived by transferring thawing blastocyst and 50 natural pregnancy control women were enrolled in this study.The expression of Grb10 protein was detected by immunohistochemistry and Western blot,and the expression of Grb10 mRNA was detected by Realtime PCR method.Results Comparison of two cases of gestational age,gestational age,fetal sex,fetal body weight,body length,head circumference,abdominal circumference,there were no significant differences(P＞0.05),comparison of placental area,placental weight,the difference was statistically significant(P＜0.05).Real-time PCR and Western blot results showed that,there was no significant difference in the expression of Grb10 mRNA and protein between the two groups(P＞0.05).Conclusion Blastocysts vitrification may increase the area and quality of delivery of placenta,however,there was no significant change in the expression of Grb10 in placenta.

Objective:To investigate the salvage role of anterior serratus branch of thoracodorsal artery in breast reconstruction with pedicled latissimus dorsi myocutaneous flap.Methods:The anatomical study was conducted from September 2015 to December 2019. Four fresh adult female cadavers were dissected to measure the number, length, diameter and origin of the anterior serratus branch of the thoracodorsal artery. The clinical data of patients who underwent modified radical mastectomy and immediate breast reconstruction with pedicled latissimus dorsi myocutaneous flap from March 2015 to March 2019 were analyzed retrospectively. It was found that the thoracodorsal artery was damaged during the flap dissection and harvestion. The latissimus dorsi myocutaneous flap was harvested pedicled with the anterior serratus branch of the thoracodorsal artery as the pedicle to reconstruct the breast instead. Postoperative flap survival, breast appearance and texture, cancer recurrence and donor site were followed up.Results:A total of 11 branches of anterior serratus muscle were found in 4 specimens (8 sides), with a length of (6.3 ± 1.8) cm and a diameter of (2.4 ± 0.4) mm, including 7 from the trunk of dorsal thoracic artery, 3 from the vertical branch of dorsal thoracic artery and 1 from the horizontal branch of dorsal thoracic artery. A total of 6 female breast cancer patients aged 29-61 years, mean 42.7 years, were included in the clinical cases. The course of disease ranged from 2 to 36 months [(10.7 ± 5.4) months]. The area of secondary breast defect after modified mastectomy was 14 cm×6 cm to 16 cm×7 cm, the area of latissimus dorsi flap was 13 cm×6 cm to 17 cm×6 cm. All flaps survived thoroughly, the appearance of reconstructed breast was good. The follow-up ranged from 9 to 28 months, with an average of 13.7 months. The breast appearance and texture of all patients were satisfactory, there was no local recurrence, only linear scar was left in the donor area, and the shoulder joint activity was not affected.Conclusions:The anatomy of the anterior serratus branch of the thoracodorsal artery is constant, and the anatomical basis of communicating with the thoracodorsal artery can supply blood to the latissimus dorsi muscle. The pedicled latissimus dorsi myocutaneous flap with anterior serratus branch of thoracodorsal artery can effectively ensure the flap blood supply safety, and can be used as a salvage method for breast reconstruction in the case of thoracodorsal artery trunk injury.

Objective:To investigate the salvage role of anterior serratus branch of thoracodorsal artery in breast reconstruction with pedicled latissimus dorsi myocutaneous flap.Methods:The anatomical study was conducted from September 2015 to December 2019. Four fresh adult female cadavers were dissected to measure the number, length, diameter and origin of the anterior serratus branch of the thoracodorsal artery. The clinical data of patients who underwent modified radical mastectomy and immediate breast reconstruction with pedicled latissimus dorsi myocutaneous flap from March 2015 to March 2019 were analyzed retrospectively. It was found that the thoracodorsal artery was damaged during the flap dissection and harvestion. The latissimus dorsi myocutaneous flap was harvested pedicled with the anterior serratus branch of the thoracodorsal artery as the pedicle to reconstruct the breast instead. Postoperative flap survival, breast appearance and texture, cancer recurrence and donor site were followed up.Results:A total of 11 branches of anterior serratus muscle were found in 4 specimens (8 sides), with a length of (6.3 ± 1.8) cm and a diameter of (2.4 ± 0.4) mm, including 7 from the trunk of dorsal thoracic artery, 3 from the vertical branch of dorsal thoracic artery and 1 from the horizontal branch of dorsal thoracic artery. A total of 6 female breast cancer patients aged 29-61 years, mean 42.7 years, were included in the clinical cases. The course of disease ranged from 2 to 36 months [(10.7 ± 5.4) months]. The area of secondary breast defect after modified mastectomy was 14 cm×6 cm to 16 cm×7 cm, the area of latissimus dorsi flap was 13 cm×6 cm to 17 cm×6 cm. All flaps survived thoroughly, the appearance of reconstructed breast was good. The follow-up ranged from 9 to 28 months, with an average of 13.7 months. The breast appearance and texture of all patients were satisfactory, there was no local recurrence, only linear scar was left in the donor area, and the shoulder joint activity was not affected.Conclusions:The anatomy of the anterior serratus branch of the thoracodorsal artery is constant, and the anatomical basis of communicating with the thoracodorsal artery can supply blood to the latissimus dorsi muscle. The pedicled latissimus dorsi myocutaneous flap with anterior serratus branch of thoracodorsal artery can effectively ensure the flap blood supply safety, and can be used as a salvage method for breast reconstruction in the case of thoracodorsal artery trunk injury.

Objective The aims of this study were to construct short hairpin RNA(shRNA)lentiviral vector in breast cancer T47D cells,to carry out RNA interference on lysine acetyltransferase 6B(KAT6B/MORF)gene,to down-regulate its expression and to explore its function.Methods Two pairs of single-stranded short hairpin RNA(shRNA5 and shRNA8)and the corresponding control sequences(Scramble5 and Scramble8)were synthesized based on the CDS of KAT6B gene.Polymerase chain reaction(PCR) was used to amplify double-stranded and ligated with the entry vector(pENTR/pSM2(CMV)GFP),which were subjected to a doub-le digestion(EcoRl and Xhol)linearization and homologous recombination with the entry vector(pENTR/pSM2(CMV)GFP)to obtain an entry clone containing the desired fragment.The target fragment was recombined onto the target vector(pLenti x1 puro DEST)via the LR cloning reaction of the Gateway system.The lentiviral packaging plasmids were co-transfected into HEK-293T cells with two pairs of target plasmids. The supernatant of HEK -293T cells was collected and transformed into T47D cells. The expression of KAT6B protein was detected in T47D cells by Western blot.Results The single colony obtained from the transformation was identi-fied by sequencing,which was consistent with the target sequence,indicating that the lentiviral vector had been successfully construc-ted.The expression of KAT6B protein was significantly lower in the shRNA KAT6B group than that in the control group,which indica-ted that the constructed gene silencing vector could play a role in the KAT6B gene in T47D cells.Conclusion The shRNA lentiviral gene silencing vectors of KAT6B were constructed and identified in T47D cells,which indicated that the foundation for further study

Objective To investigate the effect of transcranial magnetoelectric stimulation (TMES) on temporal lobe epilepsy induced by kainic acid (KA) in rats.Methods Sixty-two rats were randomly divided into pretreatment group (n=32) and treatment group (n=30);rats in the pretreatment group were divided into 4 subgroups,giving TMES at 0％,25％,50％,and 75％ maximum current intensity (MCI),respectively,to conform the optimal TMES current.Rats in the treatment group were divided 3 subgroups (n=10):TMES epilepsy group (giving TMES at optimal current),non-TMES epilepsy group,and control group;TMES was given 40 min/once daily for 14 d;the behavior,histological and electrophysiological changes of rats in the 3 groups were recorded,and the treatment efficacy of TMES in epileptic rats were evaluated.Results The optimal current was 50％ MCI.The frequency of epileptic waves in TMES epilepsy group was significantly lower than that in non-TMES epilepsy group ([30.210± 4.580] times/min vs.[31.380±4.247] times/min,t=3.325,P=0.001).Timm staining results indicated significant differences among the three groups (F=17.429,P=0.000),and the degree of Timm staining in the inner molecular layer of hippocampus dentate gyrus in the TMES epilepsy group was significantly slighter than that in the non-TMES epilepsy group (P＜0.05).Conclusion TMES can influence the formation of dentate gyrus neurons loop by improving molecular layer histological changes in epileptic rats,thereby reducing frequency of epileptic EEG seizures.