Objective To get more understanding on the symptom,and laboratory characteristics of nonsecretory myeloma(NSM).Methods 11 patients with NSM were examined by immunoglobulins IgG,IgA,IgM,IgD,IgE and their light chains,electrophoresis(PE),immunofixation electrophoresis(IFE)and serum free light chain(FLC).Results All 11 patients had more serious bone pains.all had osteolytic bone lesions.All patients were negative in PE,IFE,urine FLC tests.2 newly diagnosed patients had increased serum FLC,abnormal k/λ ratio.1 patient had normal serum FLC and normal k/λ ratio.All 11 patients had more than 30%bone marrow plasma cells.Their overall survivals were same with the typical multiple myeloma(MM).Conclusion Most NSM have more serious bone pains,and osteolytic bone lesions.PE,IFE,urine FLC tests are negative.Newly diagnosed or refractory patients have increased serum FLC,abnormal k/λ ratio,plateau phase patients have normal or reduced serum FLC.Most NSM have more than 30%bone marrow plasma cells.The therapy and prognosis of NSM are same with typical MM.

Objective To study the role of bone turnover markers in multiple myeloma(MM)bone disease.Methods Thirty-eight MM patients were studied.Serum and urine samples were taken before,after 3 months and 6 months therapy.Serum samples of tartrate resistant acid phosphatage isoform-5b(sTRACP-5b),bonespesific alkaline phosphatase(sBAP),osteocslcin(sOC),urine samples of N-telopeptides of type Ⅰ collagen(NTX)were measured.Results Urine NTX concentrations were significantly higher in newly diagnosed and relapsed or refractory patients than that in pleateum patients and controls.serum TRACP-5b concentrations were significantly higher in newly diagnosed and relapsed or refractory patients than that in controls.There were no statistical significance compared with pleateum patients.Serum BAP concentrations were significantly lower than that in pleateum patients and controls.Serum OC concentrations were not statistically significant among the newly diagnosed patients,relapsed or refractory patients,pleateum patients and control.In newly diagnosed patients.urine NTX levels were significantly higher in stage Ⅲ than that in stage Ⅰ/Ⅱ,serum BAP levels were significantly lower in stage Ⅲ than that in stage Ⅰ/Ⅱ.There was a positive relationship between urine NTX and serum TRACP-5b in newly diagnosed patients.Urine NTX levels were significantly decreased,and serum BAP levels were significantly increased after 3 cycles effective therapy.There was no change in serum TRACP-5b and OC.Urine NTX and serum TRACP-5b were significantly diseased,serum BAP and OC were significantly increased after 6 cycles of effective therapy.But bone lesions on X-ray did not diminish at that time.Conclusion There is a closely relationship between bone turnover markers and bone lesions in MM.The bone turnover markers may be useful in monitoring MM progress and therapy.

Objective To study the expression of E-cadherin and ?-catenin in endometrial and endometriotic tissues of patients with endometriosis (EM) and to compare their expression in endometriosis at different stages. Methods Twenty-four EM patients were enrolled and the expression of E-cadherin and ?-catenin in endometrial and endometriotic tissues were determined by immunohistochemistry method. Normal endometrial tissue from 21 individuals was included as the control group. Results Compared with the control group, in E-cadherin and ?-catenin expression was shown in no difference between the secretory and the proliferative phase of endometrial tissue from EM patients. Furthermore, the expressions of E-cadherin and ?-catenin in normal endometrial tissue were stronger than that of EM patients(P

Objective To explore the differentiation potency of ES cell-derived epidermal stem cells compounded by dermal analogs reconstructed with bone MSCs in hypodermis.Methods The dermal analogs were reconstructed with rat bone MSCs and compound gel-gelatin sponge.E14-ES cells,labeled with Hoechst 33342,were cocultured with human amnion.Four days later,epidermal stem cell clones were formed.The skin analogs,reconstructed with ES cell-derived epidermal stem cells and dermal analogs,were transplanted into 129 mice hypodermis.The differentiation tissue of skin analogs was sampled at 2,4,6,8 weeks.The sections were observed with HE staining,immunohistochemical and di-labeled immunofluorescence methods to test the expression of ?1 integrin,CK15,CK19,CEA,CK18.Results The sections were showed tubular or follicular like structures formed with simple or stratified epithelium at 2 and 4 weeks.Keratinized stratified squamous epithelium,sweat glands-like,sebaceous glands-like and hair follicles-like structures were observed at 6 week and 8 week after transplantation.The cells labeled by Hoechst 33342,formed tubular or follicular like structures,expressed?1 integrin,CK15,CK19,CEA and CK18positive respectively at 2 and 4 weeks.The sweat glands-like structure expressed CEA and CK18 positive respectively at 6 and 8 weeks.There were more sebaceous glands-like structures.Conclusion ES cell-derived epidermal stem cells compounded by dermal analogs reconstructed with bone MSCs can differentiate into keratinized stratified squamous epithelium,sweat glands-like,sebaceous glands-like and hair follicles-like structures in hypodermis.

Objective To evaluate whether ?G-455A gene polymorphism increases the risk of coronary heart disease(CHD) due to elevated plasma fibrinogen.Methods A total of 1 485 patients who had received coronary angiography due to chest pain or suspected cardiac ischemia by non-invasive examination were included in the study.According to the angiographic results,all the patients were divided into the control group(n=466) and coronary heart disease group(n=1 019).Patients in the coronary heart disease group were further divided into stable angina pectoris group(SAP,n=674) and acute coronary syndrome group(ACS,n=345) according to their clinical presentation.We investigated G-455A polymorphism of ? fibrinogen gene and plasma fibrinogen level in all the patients.Results Increased plasma fibrinogen levels were observed in CHD groups compared with controls(ACS: 380.92?92.35 mg/dL,SAP: 352.49?94.89 mg/dL,control: 311.72?87.09 mg/dL,P

To identify the factors that affect the hospital social responsibility(HSR)level based on theoretical analysis.79 TCM hospitals in Jiangsu Province were surveyed with questionnaires,with the data analyzed by the multiple linear regression model.The research results show the following: 1. Hospital’s financial performance,hospital size,and the congress of medical workers are positive factors to the HSR level.2.Pressure from HSR performance of neighboring hospitals has a significant negative impact.3.Hospital’s financial performance and hospital size have a significant positive impact on its intention to fulfill HSR.4.Hospital location has a significant negative impact on its intention to fulfill HSR.

Objective To study the relationship between hospital attributes and hospital social responsibility (HSR)behaviors,and measures to improve HSR performance.Methods 79 TCM hospitals in Jiangsu Province was surveyed with questionnaires for their HSR performance in 201 1 and 2012,with the data analyzed by independent two-sample t-test.Results In terms of HSR contribution,different levels of hospitals have statistical significance in bad debt,accomplishment of government mandatory missions,environmental protection,education and research,and social welfare activities.Hospitals in different regions have statistical significance in education and research,low-profit medical services and cheap drugs provision.Teaching hospitals make statistical significance in accomplishment of government mandatory missions,environmental protection,education and research,and social welfare activities.In terms of HSR willingness,different levels of hospitals have statistical significance in accomplishment of government mandatory missions,price discount,and education and research.Hospitals in different regions have statistical significance in health services with positive social effects,education and research,and social welfare activities.Teaching hospitals make statistical significance in education and research. Conclusions Secondary hospitals need to invest more resources on HSR.Hospitals in underdeveloped areas must pay more attention to environmental protection,education and research.Non-teaching hospitals need to strengthen quality of care,accomplishment of governmental mandatory missions and environmental protection.

Multiple myeloma is one of the common hematological malignant disease,but the treatment response was not very promising with conventional and high-dose chemotherapy.There are many new medicines or new therapies having been developed in recent years,such as thalidomide and its derivant,proteasome inhibitors,arsenic trioxide etc,they are more effective than conventional chemotherapy,such as oral melphalan and prednisone(MP);vincristine,doxorubicin,and dexamethasone(VAD).This paper summarize the recent advances in the new options for the treatment of multiple myeloma

The paper reviewed the main methods for the determination of paralytic and diarrhoetic shellfish toxins,including mouse bioassay,immunoassay and high performance liquid chromatography coupled with multiple techniques and so on,and summarized the principles,advantages and disadvantages of these methods.Some newly developed techniques and the development tendency for the detection of paralytic and diarrhetic shellfish poisoning toxins were also described in this paper,in order to give some reference for the detection of marine food in our country.

Objective:To detect the expression of ALDH1 and ABCG2 in the specimens from different levels of cervical lesions.Methods:Use SP immunohistochemistry to detect the expression of ALDH1 and ABCG2 in 76 cases of cervical squamous cancers,52 cases of HSIL,and 37 cases of chronic cervicitis tissues,and analyze the results.Results:①The positive expression rates of ALDH1 and ABCG2 in chronic cervicitis tissues were 5.41％ (2/37) and 8.11％ (3/37),in HSIL tissues were 23.08％ (12/52) and 28.85％ (15/52),and in cervical cancers tissues were 53.95％ (41/76) and 61.84％ (47/76),respectively.The positive expression rates in cervical cancers tissues were significantly higher than in HSIL and Cc hronic cervicitis tissues,(P ＜ 0.05).②The positive expression of ALDH1 in cervical cancer tissues had correlation with tumor grade and lymphnode metastasis (P ＜0.05),while the positive expression of ABCG2 in cervical cancer tissues had correlation with tumor grade,clinical stage and lymph-node metastasis(P ＜0.05).③The expressions of ALDH1 and ARCG2 in cervical cancer were positive relative(r =0.535,P ＜ 0.05).Conclusions:The increased expressions of ALDH1 and ABCG2 in cervical squamous cancer may play a role in the occurrence,development and metastasis of cervical squamous cell carcinoma.ALDH1 and ABCG2 may have synergistic effect in occurrence of cervical cancer.