Objective To understand the effect of curcumin and TSA on c-myc gene expression in human gastric cancer and to explore the dynamic balance of the histone acetylation / deacetylation in gene expression in gastric cancer and the significance of the regulation. Methods Gastric cancer cell line SGC-7901 and MGC803 were cultured,and then different concentrations of histone acetylase inhibitors curcumin and histone deacetylase inhibitor TSA were added,respectively.mRNA of c-myc expression wasdetected by RT-PCR. Results With increasing of the concentration of curcumin and redcuing of the TSA concentrations c-myc expression was inhibited (P

Objective To investigate the effect of Helicobacter pylori infection on cell proliferation and apoptosis of gastric cancer cell line SGC 7901. Method SGC 7901 cells were incubated with 1 × 108,5 × 107,1 × 107,5 × 106 cfu / ml concentration gradient of the standard HP NCTC 11637 strains in vitro. Morphological changes of cells were observed at 24, 48, 72 hour, respectively. Cell proliferation inhibition rate were detected with MTT assay. Apoptosis were observed by flow eytometry and TUNEL analysis, mRNA expression of survivin were detected by RT-PCR, and survivin expression were determined by western blot. Results Cell proliferation inhibition rate was 54.5%, 58.9%, 67.6%, 72.9% with 1 × 108,5 × 107, 1 × 107,5 × 106 cfu / ml concentration gradient of the HP bacteria on the role of SGC 7901 cells at 72h, respectively. The rate of apoptosis after 72h detected by flow cytometry and TUNEL with HP concentration gradient of different bacteria were 42.51%, 45.67%,48.57%, 49.51% and 54.61%, 51.26%, 59.41%, 62.46.% Helieobacter pylori could significantly reduce mRNA and protein expression of survivin. These effects were strengthened as the concentration of Helicobacter pylori increased and the time extended. Conclusion H. Pylori infection may be through reducing the expression of survivin to inhibit cell proliferation and pro-mote apoptosis of SGC 7901 in vitro. Moreover, this effect was positive related with time and dose.

Objective To evaluate the safety and efficacy of endoscopic implantation of self-expandable metallic stent (SEMs) for malignant colorectal obstruction. Methods A total of 108 patients who had undergone endoscopic SEMs implantation for malignant colonic obstruction from January 2011 to May 2014 were enrolled. The clinical suc-cess rates and the complications were reviewed. Results The clinical success rates were 92.59%(100/108). Abdomi-nal pain, perforation and bleeding were the most common post-procedure complications, the rates of which were 16.67% (18/108), 7.41% (8/108), 6.48% (7/108), respectively. The abdominal pain in most patients was self-reliev-ing except for 6 patients with perforation of colon. Patients with perforation were cured by emergency surgery. The 7 patients developing bleeding recovered themselves. Conclusion The success rate of endoscopic SEMs implantation is satisfactory in the study. As a bridge to surgery or a palliative care method, endoscopic SEMs implantation is effec-tive and safe for malignant colorectal obstruction.

OBJECTIVE:To improve the related standards in China Pharmacopoeia and to enhance its operation.METH_ ODS:Issues on“general rules on preparations”,“bacteria endotoxin test”,assaying of glucose injection in China Pharmacopoeia were analyzed and discussed.RESULTS&CONCLUSION:The dosage forms recorded are limited and some varieties of preparations are hard to be classified.How long exactly the gel formed by tachypleus amebocyte lysate and bacteria endotoxins remained cemented should be regarded as positive,which should be clearly defined.In determining the optical rotation by po?larimetry,ammonia test solution should be added to the semi-products to achieve balance but not in the finished products for differentiation.

OBJECTIVE:To investigate the problems in the publicity materials of drugs and to discuss the solutions to these problems.METHODS:35copies of publicity materials were randomly chosen from our hospital's pharmacy and were compared with the drug instructions provided by the same manufacturers.Indication,dosage and administration were the main items compared.RESULTS:Inconsistency was found in8out of35copies(22.86%),which was manifested in three aspects:widening of indications,increase in dosages,and inconsistency in administration.CONCLUSIONS:It is suggested that the technology of manufacturers and the comprehensive quality of salesmen should be improved basically.Furthermore,the su?pervisory administration should establish the law to limit drug promotion sales.

Objective To study the effects of bortezomib on the expression of NF-κB, IκB and P-gp of drug-resistant K562 cells induced by daunorubicin (K562/DNR), to explore the molecular mechanism of drug-resistant reverse. Methods The expression of NF-κB, IκB and P-gp in K562/DNR cells were detected when the cells had been treated with 100 μg/ml DNR only or together with 4 μg/L bortezomib for 12 h, 24 h and 36 h. The apoptosis rates were detected in each group respectively and the activity of NF-κB was detected by ELISA method. Results Compared with the control group, the expressions of NF-κB and P-gp in K562/DNR could be increased and IκB was decreased after being treated with DNR. When K562/DNR were cultured with bortezomib, the expressions of NF-κB and P-gp induced by DNR were significantly suppressed and IκB was increased. The activity of NF-κB were detected in different time points: (15.3±1.87) %[(23.8± 2.27) % in DNR group] at 12 h, (10.2±1.69) % [(25.4±1.98) % in DNR group] at 24 h, (6.08±2.53) % [(26.9±2.58) % in DNR group] at 36 h. There were a significant differences between DNR group and DNR+PS-341group. The apoptosis rates were increased in DNR+PS-341 group at different time points than those in DNRgroup, (35.23±5.15) % [(15.56±4.12) % in DNR group] at 12 h, (40.26±6.89) % [(17.25±2.89) % in DNR group] at 24 h, (43.58±7.69) % [(22.47±4.58) % in DNR group] at 36 h. The effccts showed the character of time-dependent pattern. Conclusion Bortezomib could downregulate the expressions of NF-κB and P-gp in K562/DNR, reverse the drug resistance and up-regulate the apoptotic rates in K562/DNR cells.

Objective To explore the clinical efficacy and complications of transoral endoscopic peroral endo﹣scopic myotomy (POEM) for achalasia (AC). Methods 38 patients with AC received POEM treatment from January 2013 to January 2013 in our digestive endoscopy center. Procedure-related complications and gastroesophageal re﹣flux were observed, and ECKARDT score and the lower esophageal sphincter pressure changes were analysed. Results All patients underwent POEM successfully. No serious POEM-related complications were observed, bleed﹣ing, gas related complications were treated successfully by conservative treatment. Postoperative follow-up time was 10.4 months (range 9 to 12 months), the symptoms of all the patients were alleviated, ECKARDT score average from preoperative 8.7 points dropped to postoperative 1.2 points (P<0.01), esophageal sphincter pressure decreased sig﹣nificantly, mean pressure dropped from preoperative (33.40 ± 11.80) mmHg to postoperative (13.50 ± 4.30) mmHg (P< 0.01) and gas related complications occurred in 6 cases (15.78 %), esophageal reflux occurred rate total was 23.68%(9/38). Conclusion POEM is safe and effective for the treatment of AC, and has better long-term effect.

OBJECTIVE:To promote the rational application of antibiotics on pediatric outpatients.METHODS:Considering the fact of our hospital,taking the acute respiratory tract infection(ARI)as an example,and using retrospective document for analysis from the aspects of the dominant idea in medication,varieties of antibiotics,the ways of administration,the course of treatment and etc.RESULTS:Subjective mistakes gave rise to the abuse of antibiotics;the variety of pediatric drugs was limited by the adverse effects of antibiotics and the diversify of drug resistance;the situation of drug abuse by intravenous drop was serious; the main cause for course shortage was due to the particularity of ambulatory treatment,uncertainty of etiology and drug resistance.CONCLUSIONS:The rational application of antibiotics on pediatric outpatients can be improved by enhancing the publicity of rational drug use,writing drug administration guidance for pediatric antibiotics applicable to the local area,developing more specifications and dosage forms.

Pyroptosis is a kind of proinflammatory programmed cell death mediated by inflammasome.It affects the occurrence and development of gastric cancer through different ways,showing dual effects.On the one hand,inflammasome-mediated inflammatory response is highly likely to participate in the formation and development of early tumors;on the other hand,drugs can inhibit the deterioration process of tumor proliferation,invasion and metastasis through activating the pathways of inflammasome and pyroptosis.Recently,many agents based on pyroptosis have been found to inhibit gastric cancer by promoting the secondary pyroptosis pathway,regulating NOD-like receptor family pyrin domain containing 3(NLRP3)inflammasome and inhibiting caspase-1.The establishment of cell pyrodeath models can predict the prognosis of gastric cancer patients.Most of the models show that gastric cancer patients with high pyroptosis level have better prognosis and longer overall survival.Pyroptosis scores can also be used to predict the response of gastric cancer patients to immunotherapy and to screen potential anti-gastric cancer drugs.Therefore,in-depth understanding of the potential mechanism of pyroptosis affecting the progression of gastric cancer and the role of pyroptosis score in the treatment and prognosis assessment of gastric cancer will be helpful to find a new and effective method for the treatment of gastric cancer.