This article is to introduce our experience in the application of random controll blind trial technique in experimental teaching of pharmacology.

Objective To understand the effect of the resect margin(RM)to hepatectomy for primary hepatocellularcarcinoma(PHC).Methods In 544 cases,72 cases were small PHC(≤3cm),473 cases were large PHC(＞3cm). Four groups were classified according to the distance of RM The 5-year recurrence rate and survival rate after hepatic resection in each group were analyzed. Results RM with residue cancer or cancer embolus had a high 5-year recurrencre rate and lower survival rate in both small and large PHC. Small PHC with RM≥1.0cm and large PHC with RM≥2.0cm had higher 5-year survival rate and lower recurrence rate. Conclusion We recommend RM≥1.0cm for smallPHC and RM≥2.0cm for large PHC.

Manned space environmental simulation technology is a very important branch of Space Medico-Engineering.After introducing the principles and methods of manned space environmental simulation technology,the current development of artificial atmosphere environment,space environment,dynamic environment,weightlessness environment and products of manned space environmental simulation technology were reviewed.

The content and thought on gradually exploratory experiment teaching of pharmacology was introduced to research the cultivation of innovative talents and good experiment concept.

The paper reviewed the main methods for the determination of paralytic and diarrhoetic shellfish toxins,including mouse bioassay,immunoassay and high performance liquid chromatography coupled with multiple techniques and so on,and summarized the principles,advantages and disadvantages of these methods.Some newly developed techniques and the development tendency for the detection of paralytic and diarrhetic shellfish poisoning toxins were also described in this paper,in order to give some reference for the detection of marine food in our country.

Objective To explore the best anticoagulant ratio in SD rats .To analyse the influence of insufficient blood specimens volume on coagulation tests .Methods 60 rats were divided into 2 groups.According to the method of vacuum blood, collect abdominal aortic blood after fasting 12 hours.The first group 20 rats were used only for routine blood test.Fully automatic hematology analyzer detected hematocrit and platelet .The second group 40 rats were used for coagulation test .Every rat was collected 2 blood specimens with different anticoagulant ratio [ the proportion of sodium citrate anticoagulation and the whole blood (vlume∶volume)]1∶9(the control group)and 1∶5( the experimental group),1∶8( the experimental group) and 1∶7( the experimental group).Get plasma without platelet through centrifugation.Fully automatic blood coagulation analyzer detected prothrombin time , activated partial thromboplatin time , thrombin time and Fibrinogen.Results HCT (%) and PLT (x 109/L) in SD rats were respectively 41.7 ±2.9 and 1114 ±173.As anticoagulant ratio was increased ,PT, APTT and TT were extended and FIB was decreased .Compared with the control group ,these PT,APTT,TT,FIB four results of 1∶8 group were not statistically different , of 1∶5 group were statistically different, these PT,APTT,FIB three results of 1∶7 group were not statistically different , TT of 1∶7 group was statistically different.Conclusions The detection of coagulation project coagulation test results were affected by the proportion of anticoagulant and blood .1∶9 was the best anticoagulant ratio in SD rats , 1∶8 can also.Rat had its unique physiological characteristics .The results could provide reference for the evaluation of rats .

10.3969/j.issn.2095-4344.2013.26.022

Objective To provide a new way for treatment of full thickness skin defects by embryonic stem(ES) cell-derived epidermal-like stem cells. Methods Epidermal like stem cells,labeled by Hoechst 33342 and carried by a layer of biomembrane,were transplanted into the defective skin of mice.The differentiation tissue of donor cells was sampled each week.The sections were observed with HE staining,immunohistochemical and di-labeled immunofluorescence methods to test the expression of ?1 integrin,CK15,CK19,CK10,CEA. Results The full thickness skin defects were healed in 2 weeks.The newborn skin was thicker than the normal skin.The basal layer cells proliferated.There were more bulky cellular poles towards dermis.The cells labeled by Hoechst 33342,located in the newborn epidermis and tubular or follicular structures in dermis,expressed ?1 integrin and CK15 positive respectively in the first 3 weeks.There were sweat gland-like,sebaceous gland-like and hair follicle-like structures in the newborn dermis after 4 weeks.Basal cells of keratinized stratified squamous epithelium expressed CK19 and CK10 positive respectively and sweat gland-like structure expressed CEA positive.Conclusion ES cell-derived epidermal stem cells can restore mice full thickness skin defects.There are epidermis,sweat gland-like,sebaceous gland-like and hair follicle-like structures in the newborn skin.

Objective To explore the differentiation potency of ES cell-derived epidermal stem cells compounded by dermal analogs reconstructed with bone MSCs in hypodermis.Methods The dermal analogs were reconstructed with rat bone MSCs and compound gel-gelatin sponge.E14-ES cells,labeled with Hoechst 33342,were cocultured with human amnion.Four days later,epidermal stem cell clones were formed.The skin analogs,reconstructed with ES cell-derived epidermal stem cells and dermal analogs,were transplanted into 129 mice hypodermis.The differentiation tissue of skin analogs was sampled at 2,4,6,8 weeks.The sections were observed with HE staining,immunohistochemical and di-labeled immunofluorescence methods to test the expression of ?1 integrin,CK15,CK19,CEA,CK18.Results The sections were showed tubular or follicular like structures formed with simple or stratified epithelium at 2 and 4 weeks.Keratinized stratified squamous epithelium,sweat glands-like,sebaceous glands-like and hair follicles-like structures were observed at 6 week and 8 week after transplantation.The cells labeled by Hoechst 33342,formed tubular or follicular like structures,expressed?1 integrin,CK15,CK19,CEA and CK18positive respectively at 2 and 4 weeks.The sweat glands-like structure expressed CEA and CK18 positive respectively at 6 and 8 weeks.There were more sebaceous glands-like structures.Conclusion ES cell-derived epidermal stem cells compounded by dermal analogs reconstructed with bone MSCs can differentiate into keratinized stratified squamous epithelium,sweat glands-like,sebaceous glands-like and hair follicles-like structures in hypodermis.

0.05).The results differentiated by Sysmex XS-800i showed excellent correlation with those by handwork.Conclusion The performance of the Sysmex XS-800i automated hematology analyzer is satisfied.