Objective To discuss the recent effect of professional instruction on patients with type 2 diabetes mellitus. Methods 200 patients with type 2 diabetes mellitus were divided into the observation group and the control group. The observation group received professional instruction while the control group only got doctors'simple advice about the characteristic, results and attentions of diabetes mellitus. Various in dices were determined before and 3 months after intervention and patients compliance was evaluated 3 months after intervention. Results The indices such as blood glucose,glycosylated hemoglobin, urine trace protein, blood lipid, weight index, blood pressue and waistline were different between the two groups(P＜0.05) after intervention although no difference was seen between them before intervention(P＞0.05).The patients compliance in the observation was better compared with the control group(P＜0.01). Conclusion Application of professional instruction proved to be an effective method to alleviate the symptoms of patients with type 2 diabetes mellitus.

In traditional Chinese medicine,Lonicerae Japonicae Flos is commonly used as anti-inflammatory,antiviral,and antipyretic herbal medicine,and geo-authentic herbs are believed to present the highest quality among all samples from different regions.To discuss the current situation and trend of geo-authentic Lonicerae Japonicae Flos,we searched Chinese Biomedicine Literature Database,Chinese Journal Full-text Database,Chinese Scientific Journal Full-text Database,Cochrane Central Register of Controlled Trials,Wanfang,and PubMed.We investigated all studies up to November 2015 pertaining to quality assessment,discrimination,pharmacological effects,planting or processing,or ecological system of geo-authentic Lonicerae Japonicae Flos.Sixty-five studies mainly discussing about chemical fingerprint,component analysis,planting and processing,discrimination between varieties,ecological system,pharmacological effects,and safety were systematically reviewed.By analyzing these studies,we found that the key points of geo-authentic Lonicerae Japonicae Flos research were quality and application.Further studies should focus on improving the quality by selecting the more superior of all varieties and evaluating clinical effectiveness.

Objective To discuss the effects of narrative medicine on diabetic foot knowledge level and metabolic parameters of patients with middle or high risks for diabetic foot. Methods We selected 90 patients with mid-and-high risks for diabetic foot who accorded with the inclusion criteria from October 2012 to May 2013. All patients were given a questionnaire about knowledge of diabetic foot to evaluate the degree of patients′mastery level of diabetic foot knowledge and after routine education, patients were randomly divided into experimental group and control group by random number table. The patients of control group received conventional education, while the patients of experimental group were intervened with narrative medicine education. We compared patient′s foot related knowledge level and metabolic parameters after 6 months. Results A total of 87 patients completed the whole study. Forty-three patients were in the control group, and forty-four patients were in the experimental group. After interventions, patient′s systolic pressure, diastolic pressure, 2 h postprandial blood sugar, and glycosylated hemoglobin in the experimental group were lower than that of the control group (P<0. 05). After interventions, the scores of the questionnaire were higher than that of the control group (P <0. 05). After interventions, the basic information of diabetes knowledge, recognition of diabetic food, food nursing, correct knowledge of wearing shoes and socks, knowledge of avoiding food injuries, total score of food knowledge questionnaire in the experimental group were compared with those of control group with statistical significance (P<0. 05). Conclusions Narrative medicine can improve middle or high risks diabetic foot patient′s foot related knowledge level and metabolic parameters.

The application of elicitors, which is currently the focus of research, has been considered as one of the most effective methods to improve the synthesis of secondary metabolites in medicinal plants. Biotic and abiotic elicitors can regulate the secondary metabolic pathways of effective ingredients in Salvia miltiorrhiza. This paper has introduced the research progress about the induction and the regulation mechanism of S. miltiorrhiza by elicitors.
Biosynthetic Pathways ; drug effects ; Drugs, Chinese Herbal ; pharmacology ; Salvia miltiorrhiza ; drug effects ; metabolism

Objective To explore the application of Pareto analysis method to assess the negative feeling of caregivers for Alzheimer′s disease ( AD) patients, to acquire the main issues and demands to intervene AD caregiver for psychological and affection support, and minimize negative feeling.Methods We surveyed forty six AD caregivers to find out their main issues and demands by return visit and carried out corresponding nursing measures.Pareto analysis had been utilized for statistical analysis.Six months later, caregivers had been investigated again and the results before and after intervention were compared.Results Before intervention, A ( sense of heavy burden ) , B ( social detachment ) , C ( physical fatigued ) accumulated to 49.82%, while D (family tensions) and E (the shortage of social support) occupied 28.57%(25%-75%), and F (the shortage of knowledge) and G (averseness to patients) held 22.61%(<25%).After six months, nurses who selected A decreased from 46 case times to 30 case times, B from 45 case times to 29 case times, C from 45 case times to 25 case times, and the time cases of all these three options reduced from 136 to 84. Conclusions By application of Pareto analysis to assess the statistic of AD caregiver′s negative feeling, it can find out the main issues, release psychological stress for caregivers, enhance quality of life, and provide liable evidence for their physical and psychological demands.

BACKGROUND: Tissue engineering is increasingly viewed as a promising avenue for functional cartilage reconstruction.However, chondrocyte dedifferentiation during in vitro culture remains an obstacle for clinical translation of tissue engineered cartilage. Re-differentiated induction have been employed to induce dedifferentiated chondrocytes back to their original phenotype. Regrettably, these strategies have been proven to be only moderately effective. METHODS: To explore underlying mechanism, RNA transcriptome sequencing was conducted on primary chondrocytes (P0), dedifferentiated chondrocytes (P5), and redifferentiated chondrocytes (redifferentiation-induction of P5, P5.R). Based on multiple bioinformatics analysis, LGR5 was identified as a target gene. Subsequently, stable cell lines with LGR5 knocking-down and overexpression were established using P0 chondrocytes. The phenotypic changes in P1 and P5 chondrocytes with either LGR5 knockdown or overexpression were assessed to ascertain the potential influence of LGR5 dysregulation on chondrocyte phenotypes. Regulatory mechanism was then investigated using bioinformatic analysis, protein–protein docking, immunofluorescence co-localization and immunoprecipitation. RESULTS: The current study found that dysregulation of LGR5 can significantly impact the dedifferentiated phenotypes of chondrocytes (P5). Upregulation of LGR5 appears to activate the PI3K/AKT signal via increasing the phosphorylation levels of AKT (p-AKT1). Moreover, the increase of p-AKT1 may stabilize b-catenin and enhance the intensity of Wnt/b-catenin signal, and help to restore the dedifferentated phenotype of chondrocytes. CONCLUSION LGR5 can modulate the phenotypes of chondrocytes in P5 passage through PI3K/AKT signaling pathway.