Objective: To investigate the effects of Chaishao Rupi Granules on the levels of serum hormones and the expression levels of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) proteins in breast tissue of the model with mammary gland hyperplasia rats, and to clarify its therapeutic mechanism. Methods: Sixty female SD rats were selected and randomly divided into control group, model group, tamoxifen group (4 mg · kg-1), and low (0.45 g · kg-1), medium (0. 90 g · kg-1) and high (1.80 g · kg-1) doses of Chaishao Rupi Granules groups. The rats in control group were intraperitoneally injected with normal saline (0. 05 mL), and fed with normal diet; the rats in other groups were intraperitoneally injected with 0. 5 mg · kg-1 estradiol benzoate for 25 d, then they were intraperitoneally injectcel with progesterone injection 5 mg · kg-1 for 5 d to establish the rat models of mammary gland hyperplasia, continuous administration for 8 weeks. The diameters and heights of the second nipples of the rats were measured, the blood of abdominal aorta was taken and the levels of estradiol (E2), progesterone (P), luteinizing hormone (LH), follicle stimulating hormone (FSH), prolactin (PRL), gonadotropin-releasing hormone (GnRH) and the densities of VEGF and bFGF were detected with ELISA. Immunohistochemistry was used to detect the expressions of VEGF and bFGF in breast tissue of the rats in various groups. Results; Compared with model group, the acinar shapes in low, medium, and high doses of Chaishao Rupi Granules groups were regular, the secretion was reduced, and the degrees of cell proliferation were reduced. Compared with model group, the heights and diameters of nipples of the rats in low, medium, and high doses of Chaishao Rupi Granules groups were significantly decreased (P<0. 05), the levels of E2, LH, PRL, GnRH, VEGF and bFGF in serum were significantly decreased (P<0. 05), and the levels of serum P and FSH were significantly increased (P<0. 05). Compared with model group, the expression levels of VRGF and bFGF in breast tissue of the rats in low, medium, and high doses of Chashao Rupi Granules groups were significantly decreased (P<0. 05). Conclusion: Chaishao Rupi Granules have obvious therapeutic effect in the mammary hyperplasia model rats, and its mechanism is to regulate the serum levels of multiple hormones and the expression levels of VEGF and bFGF proteins in brease tissue of the rats to treat the mammary gland hyperplasia.

Objective To evaluate the effectiveness and safety of Yiyang Pill in preventing and treating cardiovascular adverse reactions in patients with traditional Chinese medicine(TCM)syndrome with qi and yin deficiency and thyroid stimulating hormone(TSH)inhibition after differentiated thyroid cancer(DTC)resection.Methods A randomized,double-blind,placebo-controlled clinical trial was conducted,and 120 patients with TSH inhibition after DTC surgery were enrolled and randomized into two groups in a 1∶1 ratio using SAS 9.4 software generated random tables.The control group received a placebo and TSH suppression therapy,whereas the treatment group received the Yiyang Pill and TSH suppression therapy.The treatment period was 3 months.The incidence of cardiovascular adverse reactions,blood pressure,blood lipids,thyroid function,the dosage of levothyroxine,the efficacy of TCM syndrome,and safety indicators were compared between the two groups.Multivariate Logistic regression was used to analyze the influencing factors of cardiovascular adverse reactions.Results The incidence of cardiovascular adverse reactions in the treatment group was lower than that in the control group(P<0.05),and the efficacy of TCM syndrome treatment was significantly higher than in the control group(P<0.05).The free tetraiodothyronine level in the treatment group was higher than that before treatment(P<0.05),and the systolic and diastolic blood pressure in the control group increased compared to those before treatment(P<0.05).No severe adverse events were observed in either group.Compared with the control group,the cardiovascular incidence in the treatment group was lower,and the cardiovascular incidence in the<100 μg/d group was lower than that in the group with≥100 μg/d before treatment.Conclusion The Yiyang Pill can reduce the incidence of cardiovascular adverse reactions in patients after DTC surgery,effectively improve TCM syndromes,and be safe to use.Yiyang Pill treatment is a protective factor for cardiovascular adverse reactions,and the dosage of levothyroxine≥100 μg/d was a risk factor.

To explore the pathogenesis and treatment of recurrent granulomatous mastitis based on "deficiency， toxin and blood stasis". It is believed that the main pathogenesis of recurrent granulomatous mastitis is spleen and stomach deficiency due to chronic illness， and at the same time， the persistent or intermittent presence of various causes makes the residual toxin unclear， which leads to the stagnation of local meridians and collaterals in the breast， accumulation of lumps， and then suppuration. Deficiency of qi and blood in zang-fu organs is the main cause of this disease， and residual toxin is the key factor of this disease. The treatment should focus on promoting therapy， promoting with dispersing， expelling with supplementing， supplementing with warming and dredging， dissolving toxins and releasing stasis， and the prescription is based on modified Tuoli Xiaodu Powder （托里消毒散） or self-prescribed Jiangru No.2 Formula （浆乳2号方）. Overall， the treatment should combine deficiency， toxin and blood stasis with different syndrome differentiation and treatment， reinforce healthy qi and express toxin， and activate blood circulation and dredge collaterals with flexibly modification， to promote disease healing.