Measurement of serum Fe concentration and its physiologic variation were carried out in 240 cases with sensorineural deafness and 60 healthy subjects.The mean value and standard deviation of serum Fe concentration was 148.17?37. 64?g/dl in normal individual and 115.19?16.98?g/dl in the patients with senso-rineural deafness.The difference in serum Fe concentration between the above two groups was highly significant statistically(p

To investigate the effects of iron deficiency on cochlear structure and functions, an auditory electrophysiological and histopathological study was conducted in the rats which were fed with either a basic Fe-deficient diet or a standard diet.It was found in iron-deficient rats that the incidence of threshold elevation (more than 15 dB) in both auditory brain-stem response and electrocochleogram-N1Was 31.85%, and cochlear histopathological changes were stria atrophy and reduction of spiral ganglion cells. No change was found in the controls.These findings suggest that iron deficiency could be a direct cause of sensorineural hearing loss, or could provide a pathological basis for cochlear deafness.

Objective To compare the effect of adenoidectomy with adenoidectomy under nasal endoscope in the treatment of adenoid hypertrophy.Methods 120 cases with adenoid hypertrophy were selected as the research subjects.The patients were randomly divided into two groups by random number table,and the control group(n =60) was treated with routine adenoidectomy.The surgical treatment of the observation group(n =60) was performed under nasal endoscope.Surgical treatment,surgery related indicators (operative time,the average amount of bleeding,hospitalization time and the incidence of postoperative complications) were compared between the two groups.Before and after operation,acoustic rhinometry examination of nasopharynx was used to detect the smallest cross-sectional area and compared.Results In the observation group,the operation time,the average amount of bleeding,hospitalization time and incidence rate of postoperative complication were (5.32 ± 2.05) min,(51.05 ± 8.26) mL,(8.50 ± 2.50) d,15.00％,respectively,which in the control group were (8.56 ± 2.68) min,(78.45 ± 10.15) mL,(12.00 ± 3.50) d,36.67 ％,respectively,there were significant differences between the two groups (t =7.44,15.98,6.30,x2 =7.35,all P ＜0.05).12 months after surgery,the nasal minimal cross-sectional area of nasal pharynx in the observation group was (1.99 ± 0.51) cm2,which of the control group was (1.81 ± 0.48) cm2,there was significant difference between the two groups(t =3.99,P ＜ 0.05).Conclusion Compared with conventional adenoidectomy,endoscopic adenoidectomy in the treatment of adenoid hypertrophy has good clinical curative effect,less bleeding,shorter operative time and less postoperative complications and other advantages,it is worthy of promotion.

Objective To investigate the antiplatelet aggregation activity of clopidogrel ( small dose) combined with paeonol. Methods Antiplatelet aggregation activity was evaluated by platelet aggregation induced by adenosine diphosphate in vitro, the weight of arteriovenous bypass thrombosis in rats and bleeding time in mice. Results The combination significantly inhibited platelet aggregation, and reduced formation of arteriovenous bypass thrombosis as well as prolonged the bleeding time. Moreover, the activity of combined group was more superior to clopidogrel and paeonol, respectively. Conclusion The antiplatelet aggregation activity of the combination is better than clopi-dogrel, which has bright prospects in clinical applications.

Objective To generate a comD gene knock-out mutant of Streptococcus pneumoniae,and determine the correlation of comD gene and the bacterial resistance against β-lactam antibiotics and understand the effect of closantel down-regulating comD, comE and comC mRNA levels. Methods A suicide plasmid pEVP3comD was constructed for comD gene knock-out and a comD gene knock-out mutant (comD-)was generated through homologous recombination and insertion inactivation. PCR and immunofluorescence method were used to identify the comD- mutant and real-time fluorescence quantitative PCR was applied to detect the changes of comD, comE and comC mRNA levels before and after closantel treatment in comD-mutant and wild-type strain. Double agar dilution method was performed to determine the sensitivity of comD- mutant and wild-type strain to penicillin G and cefotaxime. Results The comD gene in genome DNA of the generated comD- mutant was inactivated by sequencing and immunofluorescence detection. 50 μ mol/L or 100 μmol/L closantel had a function to down-regulate the comD, comE and comC mRNA levels ( P ＜ 0. 05) whereas 25 μmol/L closantel did not. Both the MIC values of penicillin G and cefotaxime inhibiting comD- mutant were 32 μg/ml which was significantly higher than that of wild-type strain (0.06 μg/ml and 1 μg/ml). Conclusion In this study a comD gene knock-out mutant of S. pneumoniae was successfully generated. There is a close correlation between comD gene and β-lactam antibiotics resistance of S. pneumoniae. Closantel has a function to inhibit the competence formation of S. pneumoniae through down-regulating the transcription levels of comD, comE and comC genes.

[Objective] To determine the effects against human simplex virus type I(HSV-1) and adenovirus type Ⅲ(ADV-3) anti-viral in vitro and in vivo of eye drop prepared by mixed extract of Heartleaf houttuynia and Lonicera japonica.[Methods] By using different numbers of host cells with cytopathic effect(CPE) as the observation index,models of HSV-1 infected Vero cells and ADV-3 infected HeLa cells were established to determine the anti-viral effects in vitro of HLD with different dosages.Rabbit eye conjunctivitis model infected by HSV-1 was established to observe the anti-viral effects in vivo of HLD with different dosages,and the biopsy specimens of pathogenic eye conjunctive tissue were examined by routine pathological methods.In these experiments,a commercial acilovir eye drop(ACV) was used as the control.[Results] The minimum dosages of HLD to completely inhibit the CPEs of HSV-1 and ADV-3,the host cells were 32 mg/ml and 64 mg/ml respectively.After treatment with HLD,the inflammatory reactions of rabbit eye conjunctivitis caused by HSV-1 infection were obviously weak or disappeared.The curative rates of 1,3 and 6 g/ml HLD treating experimental rabbit eye conjunctivitis were 50.0%,83.3% and 100% respectively.Furthermore,the curative effect using 6 g/ml HLD has no significant difference compared to that using ACV drop.[Conclusion] HLD has a certain anti-viral effect in vitro and a stronger anti-viral effect in vivo,indicating the potential of HLD for developing a new drug to prevent or treat viral eye conjunctivitis.

Objective To evaluate the clinical effect of emedastine difumarate eye drops combined with pranoprofen eye drops on histamine (HA), eosinophil cationic protein (ECP), leukotrienes B4(LTB4) and, IgE levels of allergic conjunctival and provide reference for clinical diagnosis and treatment.Methods 76 cases of patients with allergic conjunctivitis were randomly divided into control group and experimental group , control group were treated with fumaric acid emedastine eye drops, experimental group were treated with fumarate emedastine combined with pranoprofen eye drops, and then compare the efficacy and serum ECP, HA, LTB4 and IgE levels between two groups before and after treatment of the symptoms and signs score . Results The total efficacy of experimental group was significantly higher than that in control group (94.70%vs.81.60%)(P<0.05).The serum ECP, HA, LTB4 and IgE levels of experimental group were significantly lower than those in control group(P<0.05).Conclusion The emedastine difumarate combined with pranoprofen eye drops in the treatment of allergic conjunctivitis is better than single medication, and ECP, HA, LTB4, IgE significantly decrease.

In the present experiment the high dose of LRH-A was injected into pregnant rats on Day 9 of pregnancy. Morphology and histochemistry of corpus luteum were studied. It was found that 24 hours after injection LRH-A could effectively cause the corpus luteum to degenarate. A great many large lipid droplets occured in the luteal cells, and the activity of 3?-HSD and of SDH of these cells decreased. At the same time the activity of AcP increased in a few of luteal cells. 48 hours after injection the effects outlined above became more distinct, and the activity of G-6-PD also began to decrease. The activity of 20 ?-HSD in both control and experimental groups was negative 24 hours after injection, while the activity of the AIP increased distinctly in endothelium of the capillary network of corpus luteum. These changes indicated that the high dose of LRH-A had damaged corpus luteum and reduced its synthetic and secretory function of progesterone which, we believe, plays a main role in terminating pregnancy.

Ankle brachial index(ABI),as a non-invasive and simple detection method,can be used to accurately assess and diagnose the severities and prognosis of the diabetic lower extremity arterial disease,cardiovascular and cerebral vascular diseases.

Objective To explore clinical characteristics of chronic total occlusion(CTO) in population with routine coronary angiography,and to cumulate therapeutic experience.Methods Retrospective analysis was performed on clinical data of 57 cases with CTO selected from 825 patients undergone coronary angiography.Results Among 57 cases,the percentage of male,myocaridial infarction,diabetes mellitus,hyperlipidermia and left ventricular dysfunction was 73.7%,84.2%,22.8%,73.7% and 22.8%,respectively.The types of lesions included single branch(n=11),double branches(n=29) and three branches(n=17).Of 70 branches lesion vessels,44 were TIMI 0 of blood flow classification and 26 were TIMI1.The position of lesions occurred at left anterior descending branch(n=32),left circumflex branch(n=16),right coronary artery(n=22).The occlusion time was less than three months(n=41) and more than three months(n=29).The lengths of lesion was divided into less than 20 mm(n=43) and more than 20 mm(n=27).The shapes of terminal lesion looked like rat-tail(n=42) and blaze knife-edge(n=28).The coronary stents were performed at 57 lesions among 70 branches and the successful rate of operation was 82.9%.Conclusions Timely coronary angiography and selecting suitable treatment on different kinds of lesions for male patients with myocardial infraction,especially complicated with diabetes mellitus,might play important roles in prevention of CTO and in improvement of the successful rate of percutanous coronary intervention for the complex lesion.