1.Study on the association between CYP2E1 enzymes and susceptibility of alcoholic liver disease
Yanmei GUO ; Qin WANG ; Aihua FENG
Chinese Journal of Practical Internal Medicine 2006;0(24):-
Objective To investigate the correlation between the CYP2E1 enzymes and alcoholic liver disease.Methods Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)method was used to determine polymorphism of CYP2E1.A case control study of 183 subjects was carried out including 40 cases of alcoholic liver disease,40 alcohol dependent group,40 nonalcoholic liver disease and 63 controls.Results The frequency of B genotype or C2 allele gene was significantly higher in alcoholic liver disease than in control(P
2.Current views on surface enhanced Raman spectroscopy in microbiology.
Xiaoxiao JIA ; Jing LI ; Tian QIN ; Aihua DENG ; Wenjun LIU
Chinese Journal of Biotechnology 2015;31(5):611-620
Raman spectroscopy has generated many branches during the development for more than 90 years. Surface enhanced Raman spectroscopy (SERS) improves SNR by using the interaction between tested materials and the surface of rough metal, as to quickly get higher sensitivity and precision spectroscopy without sample pretreatment. This article describes the characteristic and classification of SERS, and updates the theory and clinical application of SERS. It also summarizes the present status and progress of SERS in various disciplines and illustrates the necessity and urgency of its research, which provides rationale for the application for SERS in microbiology.
Microbiology
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Spectrum Analysis, Raman
3.Color Doppler ultrasonography in evaluation on renal hemodynamiacs in perioperation of percutaneous nephrolithotomy
Li LIU ; Minhui QIN ; Liang MU ; Shen LI ; Aihua WEI
Chinese Journal of Interventional Imaging and Therapy 2011;08(5):371-375
ObjectiveTo evaluate the impacts of pereutaneous nephrolithotomy (PCNL) on perioperative renal hemodynamics.MethodsThe hemodynamics of operated renal arteries of 30 patients who underwent unilateral PCNL with single pole access were observed 1 day before and 5-7 days after operation with CDFI.Parameters were analyzed statistically.ResultsAfter operation,resistance index (RI) of renal arteries decreased (P<0.05).The diastolic flow statistically increased at main renal artery (MRA) of renal hilus,interlobar renal artery and interlobular renal artery (all P<0.05).After PCNL,in serious hydronephrosis patients,RI decreased (P<0.05) at segmental renal artery (SRA) and interlobar artery,end-diastolic flow velocity (Vmin) increased at interlobar renal artery (P<0.05).In moderate hydronephrosis patients,RI decreased at all renal arteries (P<0.05) after PCNL,Vmin increased at MRA and interlobular renal artery (P<0.05).In minor hydronephrosis patients,RI decreased at MRA and SRA,Vmin increased at SRA.In patients without hydronephrosis,RI changeed like serious hydronephrosis patients.ConclusionAfter PCNL,ipsilateral renal perfusion improves,renal diastolic flow increases and RI decreases.CDFI can be used to observe the blood perfusion of kidney,and provide quantitative information of renal hemodynamics.
4.Clinical values of superoxide dismutase and malondialdehyde detection in cord blood of newborns with fetal distress
Lina QIN ; Aihua FENG ; Chuanhua YANG ; Haiyan XING
Chinese Journal of Obstetrics and Gynecology 2001;0(05):-
Objective To investigate the relations between intrauterine asphyxia and peroxidation and newborn hypoxic-ischemic encephalopathy(HIE). Methods The levels of superoxide dismutase (SOD) and malondialdehyde (MDA) in cord blood of 60 newborns with intrauterine asphyxia during labor(which was divided into two groups,39 cases with asphyxia in groupⅠ, and 21 cases with asphyxia in groupⅡ),and in 30 newborns without intrauterine asphyxia(control group) were determined. The levels of SOD and MDA in cord blood of newborns with HIE were compared with those in newborns without HIE. The incidence of HIE was estimated simultaneously. Results (1) The levels of SOD were (12 896?247) U/g Hb in groupⅠ, (9846?268) U/g Hb in groupⅡ, (17 282?134) U/g Hb in control group, significantly lower in the former two groups compared with control group, while the level of SOD in group Ⅰ was higher than that in group Ⅱ(P121 min group, and the levels of SOD was (9786?249) U/g Hb.(2)The levels of MDA were (6.3?0.4) ?mol/L in group Ⅰ, (8.6?1.5) ?mol/L in group Ⅱ, and (4.1?0.5) ?mol/L in control group, significantly higher in the former two groups compared with control group (P
5.Investigation and analysis of community psychiatric stigma in schizophrenic patients
Hongying LU ; Qin YUAN ; Li TAO ; Aihua WANG ; Xuemei ZHU ; Li CHEN
Chinese Journal of Practical Nursing 2017;33(3):202-206
Objective To investigate the community psychiatric stigma among schizophrenic patients, to explore the related factors. Methods A total of 480 cases of schizophrenia patients were investigated using the method of Link series of stigma scale. Results A total of 480 questionnaires were issued, 436 valid questionnaires were recovered, and the effective recovery rate was 90.83%. These 436 cases of schizophrenia patients perceived devaluation discrimination subscale scores, confidentiality, separation, withdrawal, education scores of coping style, the score of two dimensions of emotional experience showed statistically significant difference with median score 2.50 of the scale respectively (t=-8.393-7.930, P<0.01). The scores were significantly different in age, marriage, medical treatment among patients perceived devaluation discrimination score and stigma coping scores and the stigma of the emotional experience (P<0.05 or 0.01). Conclusions There was widespread stigma in community patients with schizophrenia, suggesting that mental health intervention should be taken for timely intervention.
6.The genotype and phenotype studies of 40 Chinese patients with X-linked adrenoleukodystrophy(X-ALD)
Lili PING ; Xinhua BAO ; Aihua WANG ; Hong PAN ; Ye WU ; Hui XIONG ; Yuehua ZHANG ; Yuwu JIANG ; Jiong QIN ; Xiru WU
Journal of Peking University(Health Sciences) 2006;38(1):66-70
Obiective:To elucidate the phenotype and the genotype-phenotype correlations in Chinese patients with X-linked adrenoleukodystrophy(X-ALD).Methods:Clinical features of 40 Chinese patients with X-ALD were studied and mutation spectrums were investigated by polymerase chain reaction (PCR) and sequencing. Results:Among these patients, four were siblings from two unrelated families, the others were unrelated. There were 31 cases with childhood cerebral (CCALD), 8 cases with adolescent cerebral (ACALD) and 1 case with adrenomyeloneuropathy (AMN). Visual impairment, which presented in 12 cases (30%), was the most common initial symptom. Nine (69%) of 13 cases who had hydrocortisone and ACTH measured showed adrenal insufficiency. By follow-up date, 19 cases (47.5%) were dead. The interval from onset to death varied from 1 to 6 years and the average were 3.3 years. The mean age at death was 10.5 years. Eleven cases (27.5%) were in vegetable state. The mean interval from onset to apparently vegetable state was 2.8 years (range from 1 to 6 years). Four cases had progressive neurological disability. Four cases were lost follow-up. One case with CCALD and one case with ACALD progressed slowly. The courses of the disease of these two patients were 5 years and 15 years respectively. Thirty five mutations were identified in 40 cases. Most were located within exon 1-3 (40%, 16/40) and exon 6-8 (42%, 17/40). There is a distinct clustering of missense mutations in exon 6 (17%, 7/40). Five types of mutations were associated with CCALD, three with ACALD and a missense mutation was identified in the patients with AMN. The two patients with long disease courses had a missence mutation c.1559 T>A and a nonsense mutation c.1785 G>A respectively. The siblings with similar manifestations and onset age were observed in two families, whose mutations were c.887 A>G and c.1028 G>T. Conclusion:The phenotypes, disease severity and rate of neurodegeneration could not be predicted by the nature of mutations.
7.Efficacy and safety of multiple-dose 5-HT3 receptor antagonists in preventing multi-day-based and highly emetogenic chemotherapy-induced nausea and vomiting
Han WANG ; Hongxue WANG ; Weimin XIE ; Fanghui QIN ; Yongkui LU ; Wenxian ZHOU ; Jing TANG ; Yan LIU ; Aihua TAN
Chinese Journal of Clinical Oncology 2017;44(13):667-672
Objective:To evaluate efficacy and safety of multiple-dose tropisetron plus dexamethasone (DXM) versus palonosetron plus DXM for chemotherapy-induced nausea and vomiting. (CINV) in patients received multiple day-based highly emetogenic chemotherapy. Methods:Cancer patients who were receiving multiday-based highly emetogenic chemotherapy were randomly assigned to AB or BA groups. A randomized, cross self-control ed method was applied. Patients in AB group received palonosetron (0.25 mg) 30 min before chemotherapy on day 1 and 3 or additional day 5 in the first cycle;and with tropisetron (5 mg) 30 min before chemotherapy on day 1, 2, and 3, or sup-plementary days (day 4 and 5) in the second cycle. Patients in BA group were treated with tropisetron in the first cycle and with palonosetron in the second cycle. Tropisetron and palonosetron were administered with DXM (10 mg) on day 1, followed by additional doses (5 mg) on days 2 to 5. Palonosetron group comprised patients in the AB group in the first cycle and BA group in the second cycle, whereas tropisetron group included patients in the AB group in the second cycle and BA group in the first cycle. Efficacy and safety of tropisetron versus palonosetron in preventing CINV were evaluated. Results:Ninety-one patients were included in analyses. At day 3, 4, and 5, incidence rates of nausea in the palonosetron group reached 28.6%, 30.8%, and 24.2%, respectively, and those of the tropisetron group totaled 42.8%, 47.3%, and 39.6%, respectively (P<0.05). At day 4, 5, and 6, incidence rates of vomiting in the palonosetron group measured 28.6%, 18.7%, and 5.5%, respectively, and those of the tropisetron group reached 42.9%, 34.1%, and 14.3%, respectively (P<0.05). From day 4 to day 5, day 6 to day 7, and day 1 to day 7, the palonosetron group yielded significantly lower incidence rates of nausea and vomiting than tropisetron group (P<0.05). Rate of rescue treatment in the palonosetron group was lower than that in tropisetron group (13.2%vs. 24.2%, P=0.057). No statistical difference in toxicities was observed between the two groups. Conclusion:Palonosetron plus DXM features better efficacy than that of tropisetron plus DXM against delayed CINV induced by multiple day-based highly emetogenic chemotherapy, which was well tolerated in the two treatments.
8.Application of PDCA Cycle Management Method in Improving Colostomy Bags Replacement Skills of Colostomy Patients
Aihua WANG ; Fang FANG ; Qin LI ; Hui NIU ; Ping LI ; Dongmei WANG
Modern Hospital 2018;18(5):776-780
Objective To study the clinical efficacy of the use of PDCA cycle management method in colostomy bags replacement skills of colostomy patients in our gastrointestinal surgery department. Methods 81 colostomy patients from January to May 2017 were selected as control group with general colostomy nursing management method and 69 colostomy patients from June to October 2017 were selected as experimental group with PDCA cycle management method. Patients' colostomy bags replacement qualified rate and nurses' ARC procedure qualified rate are compared between control group and experimental group. Results The incidence of problems from experimental group (5. 7%) was significantly lower than that from control group (17. 5%) with statistically significant difference (x~ 2 = 66. 74, P<0. 01); Patients' colostomy bags replacement qualified rate from experimental group (84. 1%) was significantly higher than that from control group (60%) with statistically significant difference (x ~2 = 11. 05, P<0. 01); Nurses' ARC procedure qualified rate & excellent rate in October (100%& 75%) were significantly higher than those in June (75% & 10%). Conclusion PDCA cycle management method can significantly improve colostomy bags replacement skills of colostomy patients, improve nurses' professional skills, improve nursing quality in our gastrointestinal surgery department.
9.Correlation study between professional identity and quitintention of pediatric nurses in Tai'an city of Shandong province
Qin QI ; Ronghui ZHAI ; Shuhong ZHANG ; Chengchao ZHOU ; Aihua ZHANG
Chinese Journal of Practical Nursing 2018;34(10):782-786
Objective To investigate the status quo and influencing factors of occupational identification and turnover intention among pediatric nurses in Tai'an,and analyze the correlation between them, and provide theoretical basis for stabilizing the pediatric nursing team. Methods A cross-sectional questionnaire survey was conducted among 442 pediatric nurses from 6 hospitals in Tai'an by means of occupational identity rating scale and turnover intention scale. Results The total score of professional identity was (97.45 ± 16.37) points; there was no very low turnover intention individual; the rate of the relative lower turnover intention was 17.87%(79/442); the rate of relative higher turnover intention was 52.26%(231/442); the rate of the very higher turnover intention was 29.86%(132/442). Career identity and dimensions were negatively related to turnover intention(r=-0.476--0.136,P<0.01). Conclusions Occupational identification of pediatric nurses in Tai'an is at a moderate level and the intention to quit is strong.The higher the occupational identification is,the lower the turnover intention of pediatric nurses in Tai'an, therefore, the nursing managers should pay attention to improve the professional identity of pediatric nurses and lower their turnover intention so as to achieve the goal of stabilizing the pediatric nursing team.
10.Clinical study of Chaichuan Tongren Mixture supplemented with hysteroscopic recanalization for the patients with tubal obstructive infertility of liver depression and qi stagnation type
Haibin LI ; Fang TAN ; Yan YOU ; Aihua QIN
International Journal of Traditional Chinese Medicine 2022;44(5):507-511
Objective:To evaluate the efficacy of Chaichuan Tongren Mixture supplemented with hysteroscopic recanalization in the treatment of tubal obstructive infertility of liver depression and qi stagnation type.Methods:A total of 60 patients with tubal obstructive infertility of liver depression and qi stagnation type who met the inclusion criteria were selected, between August 2018 and June 2020, and they were divided into the observation group with 31 cases and the control group with 29 cases, according to the random number table method. The control group was treated with hysteroscopic recanalization, while the observation group was given Chaichuan Tongren Mixture on the basis of the control group. Both groups were treated for 4 months and followed up for 12 months. TCM syndromes were scored before and after treatment, and prostaglandin E 2 (PGE 2) was detected by fully automatic biochemical analyzer and levels of TNF-α, CRP and IL-6 were measured by ELISA. The tubal patency of patients after treatment was observed, the pregnancy status was recorded after the end of follow-up and the clinical efficacy was evaluated. Results:The total effective rate was 96.77% in the observation group and that in the control group was 75.86% ( χ2=4.01, P<0.01). The scores of breast distending pain, irregular menstruation, dark purple menstrual flow and lumbosacral pain in the observation group after treatment were significantly lower than those in the control group ( t values were 17.69, 21.67, 20.89, 14.67, 18.20, respectively, all Ps<0.001). After treatment, the levels of serum PGE 2, TNF-α, CRP, and IL-6 were significantly lower in the observation group than those in the control group [(65.31±6.73) ng/L vs. (87.10±8.85) ng/L, t=10.78; (6.90±0.71) ng/L vs. (11.35±1.23) ng/L, t=17.30; (2.47±0.25) mg/L vs. (5.10±0.52) mg/L, t=25.23; (12.38±1.26) ng/L vs. (30.16±3.15) ng/L, t=29.05] ( P<0.01). After treatment, there was statistical significance in the total effective rate of tubal patency of 96.8% (30/31) in the observation group compared to 79.3% (23/29) in the control group ( χ2=4.43, P<0.01). At the end of follow-up, the pregnancy rate was 71.0% (22/31) in the observation group and 44.8% (13/29) in the control group, and the difference was statistically significant ( χ2=4.21, P<0.01). Conclusion:The Chaichuan Tongren Mixture supplemented with hysteroscopic recanalization can reduce the levels of inflammatory mediators and increase the pregnancy rate of patients with tubal obstructive infertility of liver depression and qi stagnation type.