Multiple myeloma (MM) is a largely incurable B-cell hematologic malignancy. Although some patients treated with traditional therapies, such as chemotherapy and radiotherapy,can enter a remission,the disease remains incurable. One of the biggest improvements in the treatment of MM is the use of high-dose chemotherapy followed by stem cell transplantation, which have resulted in an improved remission rate, diseasefree survival and overall survival.

Objective To evaluated the effect of Total Glucosides of Paeony (TGP)combined with urokinase for the patients with acute cerebral ischemic stroke.MethodsA total of 152 patients with ACIS were divided into the control group (77 patients) and the observation group (75 patients) by completely randomized grouping method. Patients in the control group were treated with Urokinase by intravenous, and the observation group were treated with TGP combined with Urokinase. All patients were treated with thrombolysis treatment with in the 24 h after the exclusion of cerebral hemorrhage by brain CT and coagulation mechanism disorder. In the following 14 days, they were given the treatment of anti platelet aggregation, lowering blood lipid, trophic nerve and improving circulation. The National Institutes of Health Stroke Scale(NIHSS), the modified Rankin scale(mRS) and the daily life ability index(Barthel) were measured.ResultsThere were 28 (37.3%) patients with the NIHSS scores decreasing 4 points in observation group and 12 (15.6%) in control group after treatment of 7 days,and there was significant difference between two groups (χ2=8.181,P=0.004). There were 68 (90.7%) patients with mRS score less than 4 points in observation group and 51 (15.6%) in control group after treatment of 14 days,and there was significant difference between two groups (χ2=11.945,P=0.001). The NIHSS scores in the observation group was lower than control group (8.3 ± 2.2vs. 9.9 ± 2.5;t=4.192,P<0.05). The mRS score in the observation group was lower than control group (2.3 ± 0.9vs. 2.9 ± 1.1;t=3.684,P<0.01), and the Barthel index in the observation group was higher than the control group (88.7 ± 16.2vs. 77.5 ± 15.2;t=4.401,P<0.01). ConclusionsThe TGP capsules combined with Urokinase intravenous thrombolysis could improve the neurological deficit, and the ability of daily life of ACIS patients.

0.05). Conclusions Radical TUVBT can be selected as surgical treatment for muscle-invasive bladder cancer cases,especially for those who are older and weak,and not medically fit for radical cystectomy or those who refuse the open surgery.

Objective To investigate the effect of electrovaporization on prostatic tissue structure in vivo. Methods 26 cases of benign prostatic hyperplasia underwent electrovaporization of prostatic tissue,the surface area of the vaporized prostatic tissue and the depth at the central area being observed with light microscope and electron microscope. Results The vaporizing effect on tissue with pure cut current were stronger than that with pure coagulative current, the greater the output power the stronger the vaporizing effect.6 millimeter under the vaporized surface caused by pure coagulative current still revealed some histological alterations of injury,while these histological alterations in pure cut current electrovaporization was less. Conclusions These findings may have implications that in transurethral electrovaporization of prostate,injury to the adjacent or deep tissues should be avoided as much as possible.

Objective To discuss the relationship between leptin level and polycystic ovary syndrome (PCOS) in adolescent patients with polycystic ovary syndrome, and to explore the classification diagnosis method of adolescent PCOS and indicator for clinical monitoring of obese patients. Methods All enrolled adolescent individuals were assigned into four groups: 30 normal adolescent individuals in the control group, 30 simple adolescent obese individuals in the simple obesity group,27 obese adolescent PCOS patients in the obese PCOS group and 14 nonobese adolescent PCOS patients in the nonobese PCOS group. The fasting serum samples were prepared for leptin level measurement and analysis, Results The serum leptin level of in the control group, the simple obesity group, the obese PCOS group and the nonobese PCOS group were ( 19.44 ± 6. 63 ) μg/L vs.(23.09 ±7. 39) μg/L, (42. 99 ±9. 83) μg/L and (31, 92 ±7, 02) μg/L,respectively. Leptin in the obese PCOS group was significantly higher than that in the control group and the simple obese group (t = 2. 903 and 2. 714 respectively,Ps ＜ 0. 05 ). Conclusion Monitoring the serum level of leptin can not only aid the classification of adolescent PCOS patients and guide the treatment, but also can serve as a indicator for therapeutic monitoring of obese adolescent PCOS.

Objective To detect the expression of hypoxia inducible factor-lα (HIF-1α) in both mRNA and protein and reactive oxygen species (ROS) levels in human epithelial ovarian cancer,and investigate the abnormal state of oxygen metabolism and their interaction in human epithelial ovarian cancer develoment.Methods The expression of HIF-1α in mRNA and protein in epithelial ovarian cancer were detected by real time reverse transcription-polymerase chain reaction and immunohistochemical Elivision plus two-step method,and the level of ROS was detected by fluorescence microplate reader.Results The relative content of HIF-1α mRNA in the epithelial ovarian cancer group was 4.466±0.754,which was higher than that of the benign control group (6.073±1.053) (t =-7.701,P =0.000).The expression of HIF-1α mRNA was raised gradually in tissues of the benign ovarian tumor,early and advanced stage of epithelial ovarian cancer (t =5.301,P =0.000).The expression of HIF-1α mRNA in the epithelial ovarian cancer had positive correlation with grade and lymph node metastasis (t =3.724,-3.851,P =0.000).The positive expression rate of HIF-1 α protein in epithelial ovarian cancer was 63.49 ％ (40/63),which was higher than that of the benign group (U =433,P =0.005),which had positive correlation with lymph node metastasis and stage (x2 =4.289,12.360; P =0.038,0.000).The expression of ROS in the epithelial ovarian cancer was 110.239±18.414,which was higher than that of the benign group (58.366±20.360) (t =11.068,P =0.000),which had positive correlation with grade,lymph node metastasis and stage (t =-2.055,2.816,-4.138; P =0.044,0.007,0.000).There was a moderate positive correlation between the expression of HIF-1α mRNA and ROS in epithelial ovarian cancer (r =0.572,P =0.000),and that was similar in low differentiation group and advanced group,there was a relatively high positive correlation in lymph node metastasis group (r =0.703,P =0.001),there was a relatively low correlation between HIF-1α mRNA and ROS (r =0.376,P =0.002).Conclusion Hypoxia is the basic characteristic of human epithelial ovarian carcers.This study suggested that HIF-1α is associated with human epithelial overaian cancer prognosis.ROS and HIF-1α might be involved in the genesis of human epithelial ovarian cancer,and the regulation of ROS to HIF-1α may play the important roles in the invasion and metastasis process of human epithelial ovarian cancer.The expression of HIF-1α is regulated by ROS at the transcriptional and protein levels,probably mainly on protein level.

Cardio-protective action of synthetic peptide 6A, fibrinogen degradation segment, was observed on the myocardial injury model produced by subcutaneous injection of iso-proterenol (30 mg?kg-1-d-1) into rat. Treatment with peptide 6A (50 ?mol?kg-1? d-1,iv) significantly ameliorated isoproterenol-induced myocardial lesion, inhibited release of myocardial creatine phosphokinase, ?-hydroxybutyratedehydrogenase, lactate dehydrogenase and gluta-mate-oxaloacetate transaminase, lowered plasma fibrinogen content,and markedly prevented myocardial calcium accumulation. The results suggest that peptide 6A could have potential significance for clinical therapy of ischemia heart diseases.

Objective To investigate change of Th 1/Th 2 Cytokines in the rats with spleen deficiency syndrome and effect of decoction of SiJunZi on it. Methods The contents of IL-4 and IFN-? in the T cells of the rats with spleen deficiency syndrome before and after treatment of decoction of SiJunZi and powder of ChaiHuShuGan were detected by ELISA. Results Compared with normal group, the content of IFN-? decreased (P0 05). After treated with powder of ChaiHuShuGan, the levels of IL-4 and IFN-? did not obviously change compared with normal group (P

Objective To explore relationship between Liver and Spleen of Tradicational Chinse Medicine in T cell activation. Methods The expression of IFN-r and IL-4 of T cells in asthenic spleen qi model and hepatic depression model was detected by RT-PCR. Results IFN-r level of T cells in asthenic spleen qi model significantly decreased, in hepatic depression model significantly increased, in asthenic spleen qi model treated with decoction of four mild drugs rose to normal range, and in hepatic depression model treated with powder of bupleuri dispersing stagnated hepatic qi fell to normal range. The IL-4 level of T cells in asthenic spleen qi model significantly increased, in hepatic depression model significantly decreased, in asthenic spleen qi model treated with decoction of four mild drugs fell to normal range, and in hepatic depression model treated with powder of bupleuri dispersing stagnated hepatic qi rose to normal range. Conclusion Liver and spleen are related to the regulation of THO cells functions.

Objective To explore the relationship between the plasma level of chemokines and clinical condition in patients with congestive heart failure (CHF). Methods The plasma levels of monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) were measured in 62 patients with CHF (NYHA II～IV) and 18 healthy control subjects by ELISA and RIA, respectively. Results The levels of MCP-1 and IL-8 were significantly higher in CHF group than those in control group, and had a gradual increase along with the increasing of NYHA functional class. The increase of the chemokines levels was independent on the cause of the heart failure. The plasma MCP-1 level was significantly correlated with left ventricular ejection fraction, 6-minite walk distance, quality of life scores, and left ventricular end diastolic diameter, respectively(r=-0 422, -0 292, -0 421, 0 263, all P