Objective To study the effect of acupuncture and medication on plasma ealcitonin gene-related peptide(CGRP) levels in patients with acute cerebral infarction. Methods 90 patients with simple obesity were ran-domly divided into acupuncture and medication group( n = 45) and medication group( n = 45 ). All patients were ex-amined the plasma level of CGRP to observe clinical curative effect before and after 1 month treatment. Results The total effective rate of acupuncture and medication group 91.1% (41/45) was significantly higher than that in medication group 77.8 % (36/45) (P < 0.05) ; after treatment the plasma level of CGRP in two groups were signifi-candy higher than those before treatment(P < 0.05) , acupuncture and medication group was significantly higher than that in medication group (P < 0.05). Conclusion Acupuncture in combination with drug could cut down nerve cell damage, and it may be significantly related to levels of CGRP in patients with acute cerebral infarction.

Aim To observe the effect of Ginkgo biloba extract 50(GBE50)on L-type calcium current and cytosolic[Ca~(2+)]_i in ischemic guinea pig ventricular myocytes.Methods Single ventricular myocytes were isolated by enzymatic dissociation. I Ca-L was recorded by whole-cell patch clamp technique in voltage clamp mode.[Ca~(2+)]_i was detected by laser confocal micros-copy and represented by relative fluorescent intensity (FI).Results During ischemia, the peak Ca~(2+) current was reduced, and the I-U curve of I Ca-L was shifted upward.50 mg·L~(-1) GBE50 reversed the change induced by ischemia(n =6, P >0.05).After perfusing ischemic solution for 12 min, intracellular calcium concentration was increased(n =10, P <0.01).After perfusion with ischemic solution containing 50 mg·L~(-1) GBE50, the increase of intracellular calcium concentration was markedly inhibited(n =10, P >0.05).Conclusion GBE50 can reverse the decrease of I Ca-L and partially inhibit calcium overloading during ischemia.

With use of ~(125)I-udR release test,a significant natural killer activityagainst K_(562) target cells has been determined in human peripheral wholeblood(WB).r There was a gradual decrease in NK activity as rise inthe dilution or WB.In comparison with peripheral monouclear cells(PBMC)oneself,the NK activity lowered distinctly at both 1:4 and 1:8 dilution ofWB(p0.4).It seems that neither the red blood cells nor thePlasma ffeect on NK activity of WB,The NK activity in WB as well as inPBMO was improved by incubation with human leukocyte interferon.TheNK activity of WB and PBMC in patients with hepatocellular carcinomawas signiticantly lower than in normal controls.The results showed tahtNK activity may be determined by using properly diluted WB instead orPBMC,and the assay can be easily performed in clinical practice.

Objective To evaluate therapeutic effect and safety of hysteroscope operation in treating hysteromyoma. Methods 146 patients were subjected to transcervical resection of myoma (TCRM), curative effects were evaluated as satisfaction or dis-satisfaction according to the degree of bleeding control, the rate of growth of myoma and the need of subsequent surgery. Results The total satisfactory rate is 95%. and satisfactory rate of type 0 and Ⅰ reached 100%. There was no complication in all patients. Conclusion TCRM is the best choice in treating submucous myoma. The results of surgery were satisfactory to intramural myoma if gasp the indictions. Selecting proper indications for operation, B ultrasonic monitoring severity are important for ensuring the operation effect and safety.

Objective To investigate the prevalence of human immunodeficiency virus (HIV) infection and its characteristics among pregnant women in Hunan.Methods Data from information system of prevention of mother-to-child transmission of HIV management in Hunan 2011-2015 was analyzed in the study (3 + 1 mode by year statistics).Results The total HIV-positive infection rate was 0.19‰ among pregnant women from 2011 to 2015 in Hunan.The rate of HIV infection showed upward trend by years (P ＜ 0.05).The proportion of diagnosis of HIV positive cases intrapartum was 44.66％,showed declining trend by years (P ＜ 0.05).The 786 cases of HIV positive pregnant women were mainly the Han's,the age distribution of 20 to 35 years old,90.21％ of them were farmers or unemployed.A percentage (76.84％) of them had junior high school education level or lower 37.91％ of them were found in delivery or postpartum.A percentage (36.51％) of them accepted service in delivery or postpartum.A percentage (45.67％) of them was infected through sexual contact,46.82％ of them were infected by unknown ways.Conclusions The rate of HIV infection among pregnant women was increased by years in Hunan.It is suggested to strengthen health education among high-risk groups and high incidence areas,improve detection rate of early pregnant women,implement the Prevention of Mother to Child Transmission (PMTCT) measures to reduce the rate of mother to child transmission of Acquired Immune Deficiency Syndrome (AIDS).

OBJECTIVE To analyze the characters of hospital infection among deceased inpatients.METHODS The inpatients who died in hospital from Jan 2004 to Dec 2005 were analyzed retrospectively.RESULTS The infection rate of deceased inpatients was 40.11%,and the location majored in respiratory tract,urinary system and digestive tract.The infection rate was apparently higher among the cases more than 60 years old,and those with hematological disease,circulatory disease and nervous system disease.The hospital infection rate was related with long hospitalization and had seasonal fluctuation.CONCLUSIONS The high hospital infection rate among deceased pabients may be related with the seriously underlying diseases, aging,drug abuse and invasive therapy.

Objective To analyze the causes of blood transfusion adverse reactions in children,and to explore the measures to reduce the adverse reactions of blood transfusion in children.Methods Collect 30 518 cases of transfusion reaction data which is during 2009 to 2014 in Kunming Children's hospital for analyzing.Results 166 cases of transfusion reaction occurred and the occurrence rate is 0.54％ in 30 518 cases,allergic transfusion reaction accounted for 89.16％ (148/166),non-hemolytic febrile reaction 10.24 ％ (17/166),hemoglobinuria accounted for 0.60 ％ (1/166);In children the transfusion reaction rate of hemapheresis platelet is 1.92％(72/3 743),plasma is 0.43％(56/13 132),suspension red blood cells is 0.28％(38/13 480);the signs and symptoms of children's blood transfusion reaction:simplex urticaria and rash 74.10％(123/166),simplex fever 10.24％ (17/166),rash associated with fever 5.42％ (9/166),eyelid or oral edema (mild angioneurotic oedema) 9.04％ (15/166),,Bronchospasm (mild) 0.60％ (1/166),simple hemoglobinuria 0.60％ (1/166).Conclusion Children with blood transfusion adverse reactions to allergies,mainly for local or systemic rash;Platelet is the most-common blood component that causes transfusion reaction with children.

Objective To explore the clinical significance of the three indicators combined detection that plasma endotoxin,pro-calcitonin (PCT)and (1,3)-β-D-glucan(BG)in pulmonary tuberculosis patients complicated with bacterial,fungal infection.Methods Retrospective investigation was conducted in 240 pulmonary tuberculosis patients,who were divided into gram negative bacteria (G- )group(39cases),gram positive bacteria (G+ )group(45 cases)including fungal,germ-free group(156 cases).Other 45 healthy people were selected into control group.The levels of plasma endotoxin,PCT and BG in the four groups were compared.Results The levels of plasma endotoxin(0.682±0.418)EU/mL,PCT(2.93±0.87)μg/L in the G- group were significant higher than those of the G+ group (0.063±0.034)EU/mL,(0.85±0.52)μg/L,the difference was significant (P <0.05).The levels of the plasma endotoxin,PCT in the G- group were higher than those of the germ-free group.The levels of plasma endotoxin,PCT and BG in the control group had no significant difference with those of the germ-free group.Conclusion The combined detection of plasma endo-toxin,PCT and BG have some clinical value on the early diagnosis of the patients with pulmonary tuberculosis complicated with G-fungal infection for the advantages of fast and sensitiveness.

Objective To investigate the effect of umbilical cord serum(UCS)during induction of dendritic cells derived from umbilical cord blood. Methods Umbilical cord blood was collected aseptically and interface cells were collected with density gradient centrifugation method,CD34 +cells were purified and collected with Mini-MACS technique. Pro-DC were obtained and were divided into 3 groups:①cultured in RPMI-1640 medium with 10%UCS and cytokine,②cultured in RPMI-1640 medium with 10%FCS and cytokine③cultured in RPMI-1640 medium with 10%FCS .Interleukin-4(IL-4),granulocyte-macrophage colony-stimulating factor(GM-CSF)and tumor necrosis factor(TNF-a)were used in the first 2 groups, but not in control group (group 3). Some cells were collected on the 8th d for phenotypes analysis. Results The expression of CD80 ,CD54 and HLA-DR on the surface of DC cultured in UCS medium had no significant difference compared with that of DC cultured in FCS medium. The expression of surface molecules in UCS-DC group and FCS-DC group were both enhanced compared with the control group. Conclusion Mature DC could be induced from pro-DC derived from umbilical cord blood when cultured with UCS instead of FCS. That provides a new approach for the clinical application of DC.

OBJECTIVE:To evaluate the efficacy of latanoprost for patients with refractory angle-closure glaucoma in whom the intraocular tension failed to be kept under control by common anti-glaucoma drugs.METHODS:A total of 23 refractory glaucoma cases(36 eyes)whose intraocular pressure(IOP)failed to be kept under control by common anti-glaucoma drugs were assigned to receive latanoprost alone or latanoprost combined with timolol or pilocarpine for 6 months.The intraocular tension,visual acuity,visual field and side-effect were observed.RESULTS:In acute primary angle-closure glaucoma group,the mean IOP was(27.13?5.21)mmHg before treatment vs.(16.21?3.45)mmHg at six months(P