OBJECTIVE:To study the stability of ethylenediamine diaceturate(EDD)for injection and compatible stability of 3 kinds of clinically common infusions.METHODS:The experiement of influencing factors(including light,high temperature,10 days)was carried out meanwhile the accelerated test(6 months)was performed for the preparation under the condition of being packaged;the stability of EDD in 5% glucose injection,10% glucose injection or in 0.9% sodium chloride injection within 8.0 h were observed.The major outcome measures were appearance,visible foreign substances,pH value,contents of principal agent(area of the chief peak),the relative substances and 5-hydroxymethyl furfural,etc.RESULTS:No marked change was noted for all the outcome measures except a slight increase of the content of the related substances in the light exposure test.CONCLUSION:EDD for injection stored away from light at room temperature shows good stability,and it is stable within 8.0 h after mixing with 3 kinds of common infusions.

Objective To investigate the role of herpesvirus infection and the activation of Th cell subsets in rheumatoid arthritis (RA) patients.Methods Polymerase chain reaction (PCR) was used to determine herpes viral (HSV 1,HSV 2,EBV,CMV) DNA in whole blood (WB) and serum samples.ELISA used for measuring serum levels of IFN ? and IL 4.Results ① Thirty two WB samples and 32 serum samples from RApatients were tested for the viral DNA types.Of them 2 WB samples were positive for HSV 1 DNA and 1 serum sample did.In normal control samples one serum sample was positive for CMV DNA.There was no significant difference in rate of detection between control group and test group.② Comparing serum levels of IFN ? and the ratio of IFN ?/IL 4,it was found that RA patients had significantly higher values than the normal controls.Increased values directly correlated with severity of disease.[in RA patients IFN ? (21?33) pg/ml.TFN ?/IL 4 (25?20),in normal controls IFN ? (10 1?2 6) pg/ml, IFN ?/IL 4 (17?8)].Conclusion ① The rate of detection of herpes viral (EBV,CMV,HSV 1,HSV 2) DNA does not correlate with RA significantly.② Increased serum levels of Th1 derived inflammatory cytokine (IFN ?) and the impaired production of anti inflammatory Th2 derived cytokines (IL 4) indicate impaired activation of Th1 and Th2 in RA patients and may account for the chronic arthritis.

Objective To investigate the effect of cluster nursing on quality of life and mental state of cervical cancer patients receiving chemotherapy. Methods 72 cervical cancer patients receiving chemotherapy were randomly divided into control group and observation group.The control group was given routine care and the observation group was given cluster nursing on the basis of routine care.The quality of life core questionnaire (EORTC QOL-C30) and Symptom Checklist SCL-90 score were tested at the end of treatment. Results Compared with the control group, after using cluster nursing, the EORTC QOL-C30 functional areas of physical function role function, cognitive function , social function, emotional function of patients in the observation group were significantly improved( <0.05), the main symptoms of nausea areas vomiting and pain were decreased significantly ( <0.05),and sleep,diet,diarrhea and constipation were improved significantly (<0.05) . SCL-90 scale showed that the somatization,obsessive-compulsive symptoms,depression and anxiety in observation group were decreased significantly compared with the control group ( <0.05),the somatization, obsessive-compulsive symptoms and anxiety were increased significantly in control group compared with China norm (<0.05) .Conclusion Cluster nursing on patients with cervical cancer receiving chemotherapy can effectively improve quality of life and reduce the incidence of negative emotions.

Objective To detect the change of precursor protein of sol in breast cancer before and after the neoadjuvant chemotherapy,so as to find the serum protein marker of chemotherapy drug resistance in breast cancer.Methods Breast cancer patients with Ⅲ period neoadjuvant chemotherapy were enrolled in the study.The serum specimens of the patients were obtained.Two-way gel electrophoresis was used to get the gel map before and after chemotherapy,then the image map was analyzed by gel analysis software.The differentially expressed proteins point was screened before and after chemotherapy.Serum protein differences were identified with matrix auxiliary laser disi ntegrate series time of flight mass spectrometer (MALDI-TOF-MS),including coagulation sol precursor protein.Western blot was used to test the variation trend of them before and after chemotherapy.Results Coagulation sol precursor protein levels fell after neoadjuvant chemotherapy in resistance group.The variation trend of the results of western blot and that of the proteomics were consistent.Conclusion Coagulation sol precursor protein can be as the candidate serum marker of chemotherapy drug resistance in breast cancer.

Objective To explore changes of the myeloid derived suppressor cell (MDSC), regulatory T cell (Treg), traditional T cell, and their mechanisms in lung tumor mice. Methods Twenty C57BL/6 mice were randomly divided into the experimental and the normal control groups. The experimental group was injected with Lewis lung cancer cells (LLC, 100μL 1 × 106) subcutaneously to prepare the lung tumor model mice, the normal control group was given the same amount of saline (NC). Spleen cells were obtained from LLC and NC groups. Flow cytometry was used to detect the ratio and number changes of MDSC, Treg, CD4+and CD8+T cells in the lung tumor of mice. CD4+and CD8+T cell apoptosis were detected by Annexin-Ⅴstaining, and their proliferation were detected by 5-bromine deoxidization uracil nucleoside (BrdU) incorporation. Results Compared with normal control mice, the ratio and number of MDSC in spleen increased significantly in LLC group (P<0.01), in addition, the ratio of CD4+Foxp3+Treg in CD4+T cells and their number in spleen increased significantly in LLC group. However, the ratio and number of CD4+and CD8+T cells in spleen decreased significantly in LLC group (P<0.05). The proliferation of CD4+and CD8+T cells decreased significantly in LLC group compared with that of NC group (P<0.05), while the apoptosis of CD8+T cells increased significantly (P<0.05). Conclusion MDSC and Treg cells increase in lung tumor model mice, which inhibit proliferation of CD4+and CD8+T cells and promote apoptosis of CD8+T cells.

BACKGROUND:As a novel noninvasive method, high frequency ultrasound has been used for the detection of skeletal muscle widely. OBJECTIVE:To detect the thickness of the dorsalLisfrancligament between the first (medial) cuneiform (C1) and second metatarsal (M2) using high frequency ultrasound to evaluate the feasibility and clinical application value of high frequency ultrasound for the detection of theLisfrancjoint. METHODS:Both feet of 100 healthy volunteers were included and the thickness of dorsalLisfranc ligament and the distance between C1 and M2wereexamined using high frequency ultrasound. RESULTS AND CONCLUSION:The display rate of high frequency ultrasound for the dorsalLisfranc ligament in the 100 healthy volunteers was 100%. There was no significant difference in the thickness of the dorsalLisfrancligament between both feet or sexes in adults(P> 0.05); as did the distance between C1 and M2 (P> 0.05). These findings suggest that visual dorsalLisfrancligament is detected by high frequency ultrasound. The measurement of the distance between C1 and M2by high frequency ultrasound may be a feasible, noninvasive, low-cost, and early diagnostic method forLisfrancinjuries.

AIM: To verify the effect of cholecystokinin octapeptide(CCK-8) on cardiac function in endotoxin shock (ES) rats.METHODS: The rats were divided into four groups:control,lipopolysaccharide(LPS),CCK-8 and CCK-8+LPS.The left ventricle pressure(LVP),the maximal/minimum rate of LVP(?LVd p /d t max ),heart rate (HR) and mean arterial pressure (MAP) were measured.The activity of superoxide dismutase (SOD),the contents of malondialdehyde (MDA) and nitric oxide (NO) in both serum and myocardium were also measured,respectively.RESULTS: CCK-8 (40 ?g?kg -1 , iv) elicited bradycardia in short time and gently increase MAP,LVP and ?LV d p /d t max . Lipopolysaccharide(LPS, 8 mg?kg -1 , iv) caused a variation in heart rate (HR)(a bradycardia following a tachycardia) and rapid decreases in MAP,LVP and ?LVd p /d t max . The rapid variation of HR and the decline of MAP,LVP and ?LVd p /d t max were reversed by pretreatment with CCK-8 in ES rats, but didn't restore to normal.The activity of SOD was increased and the contents of MDA and NO were decreased by pretreatment with CCK-8 in ES rats.CONCLUSION: The decline of cardiac function in ES rats could be reversed by pre-administration of CCK-8 and the decrease in NO production may be one of the mechanisms.

Objective To explore the effect of p21Waf1/Cip1 methylation changes on the process of cellular senescence .Methods Bisulfite sequencing was used to analyze the methylation changes of p21Waf1/Cip1 in the process of cellular senescence;p21Waf1/Cip1 ex‐pression was detected by RT‐PCR and Western‐blot ;Middle‐aged 2BS cells was treated by 5‐aza‐CdR and cellular senescence was detected by MTT and SA‐β‐Gal staining .Results Bisulfite sequencing analysis of p21Waf1/Cip1 promoter showed that CpGs were methylated by 1 .25% in the young 2BS cells ,by 27 .27% in the middle‐aged 2BS cells ,while only by 0 .64% in the senescent cells . The expression of p21Waf1/Cip1 was low in the young(28 PD) 2BS cells ,it increased first(35 PD) but decreased later in the middle‐aged(42 PD) cells .In the senescent 2BS cells ,p21Waf1/Cip1 expression was further increased .5‐aza‐CdR treatment resulted in de‐creased growth rate but increasedβ‐Gal staining of middle‐aged 2BS cells .Conclusion The process of cellular senescence is regula‐ted by the status of p21Waf1/Cip1 methylation ,and p21Waf1/Cip1 demethylation accelerates cellular senescence .

Objective To investigate the value of Pituitrin in neonatal cardiopulmonary resuscitation.Methods Seventy-three cases with neonatal cardiopulmonary arrest admitted in emergency department and NICU in our hospital were collected during 2007 to 2011.Newborns who did not respond to conventional neonatal resuscitation therapy were divided into two groups:epinephrine group 47 cases (control group) and Pituitrin combined with epinephrine group 26 cases (treatment group).Results There were no statistical difference (x2 =0.956,P ＞ 0.05) between treatment group and control group in the rates of initial resuscitation success (23.1％,6/26 vs 34.0％,16/47).Conclusion Pituitrin combined with epinephrine has similar efficacy with the use of epinephrine in neonatal cardiopulmonary resuscitation.

Aim To investigate the effect of Dicranostiga Leptodu (Maxim) Fedde (DLF) on experimental liver injury induced by Calmette Guerin bacillus vaccine (BCG) and Lipopolysaccharide (LPS) in mice. Methods Experimental liver injury was induced by Calmette Guerin bacillus vaccine and Lipopolysaccharide in male mice by injecting via tail vein (50 ?g?kg -1 ), and administering LPS (10 ?g?mouse -1 ) 10 d later. Therapeutic groups were given respectively with DLF (0 5,1 0,2 0 g?kg -1 ) before administration of LPS. The level of ALT, AST and LDH and the concentration of ALB and GLB in serum were measured by automatic biochemistry analyzer, and MDA in liver plasma was measured by TBA method and the level of SOD was measured by pyrogallol auto oxidation method. Pathological morphological changes in liver tissue were observed. Results The three experimental groups were found with significant decrease in the elevation of serum GPT and LDH level and the content of MDA in the liver plasma. The liver tissue damages were also ameliorated. Conclusion DLF has protective effect against the experimental liver injury induced by BCG and LPS in mice.