ObjectiveTo explore application effect of predictive nursing in children severe craniocerebral injury combined with pulmonary contusion.Methods 60 children severe craniocere-bral injury combined with pulmonary contusion were divided into observation group and control group.The control group was received routine nursing while the observation group was given pre-dictive nursing.Nursing effect was compared between the two groups.Results The blood oxygen saturation in the observation group was higher than that in the control group,the infection time was longer than that in the control group,the infection rate and mortality rate was lower than that in control group,Karnofsky scores after intervention was higher than that in the control group,the hospitalization time was shorter than that in the control group,the results revealed that the differ-ence was statistical significant (P <0.05).The difference was statistical significant since nursing satisfaction in the observation group was higher than that in the control group (P <0.05).Con-clusion Preventive nursing could reduce infection in children severe craniocerebral injury combined with pulmonary contusion and improve the prognosis.

ObjectiveTo explore application effect of predictive nursing in children severe craniocerebral injury combined with pulmonary contusion.Methods 60 children severe craniocere-bral injury combined with pulmonary contusion were divided into observation group and control group.The control group was received routine nursing while the observation group was given pre-dictive nursing.Nursing effect was compared between the two groups.Results The blood oxygen saturation in the observation group was higher than that in the control group,the infection time was longer than that in the control group,the infection rate and mortality rate was lower than that in control group,Karnofsky scores after intervention was higher than that in the control group,the hospitalization time was shorter than that in the control group,the results revealed that the differ-ence was statistical significant (P <0.05).The difference was statistical significant since nursing satisfaction in the observation group was higher than that in the control group (P <0.05).Con-clusion Preventive nursing could reduce infection in children severe craniocerebral injury combined with pulmonary contusion and improve the prognosis.

OBJECTIVE To investigate whether icari-in(ICA)plays a neuroprotective role by improving glyco-lytic function through activating Wnt/β-catenin signaling pathway.METHODS HT22 cells were treated with Aβ25-35 for 24 h to establish AD cell model,ICA was added in 2 h before Aβ25-35 and the DKK1(a specific inhibitor of the Wnt signaling pathway)was added in 0.5 h before ICA.Pharmacodynamic study:HT22 cells were divided into control group,ICA group(ICA 10 μmol·L-1),model group(Aβ25-3520 μmol·L-1),model + ICA group(Aβ25-3520 μmol·L-1 +ICA 2.5,5,10 μmol·L-1);Mechanism study:HT22 cells were divided into control group,model group,Aβ25-35+ICA 10 μmol·L-1 group,Aβ25-35+DKK1 group,Aβ25-35+DKK1+ ICA group.The cell viability was detected by MTT assay and the cell morphology was obtained by microscope,the lactate content was detected by lactate assay,the ATP content was measured with the chemiluminescence method,the expression levels of HK1,PKM1 and the pro-tein expression of molecules related to the Wnt/β-catenin signaling pathway(Wnt3a,GSK3β,pGSK3β Try216,pGSK3β Ser9,β-catenin,pβ-catenin Ser33/37 Thr41,Active β-catenin and nuclear β-catenin)was assayed by Western blotting.The nuclear translocation of β-catenin was observed by immunofluorescent staining.RESULTS Compared with the control group,the viability of cells in the model group was reduced,the morphology of cells was significantly damaged,the ATP content and lactate content were significantly decreased,and the glycolytic key enzymes:the protein levels of HK1,PKM1 and the protein levels of Wnt3a,pGSK3β Ser9,active β-catenin and nuclear β-catenin were significantly reduced,and the phosphorylation levels of β-catenin Ser33/37 Thr41 were significantly increased.Compared with the model group,the cell morphology was significantly improved and the viability was significantly increased,the ATP and lactate content were significantly increased,the expressions of HK1,PKM1 and Wnt3a,pGSK3β Ser9,active β-catenin and nuclear β-catenin protein were significantly upregulat-ed,and the phosphorylation levels of β-catenin Ser33/37 Thr41 were significantly reduced after ICA treatment.However,when the canonical Wnt signaling was inhibited by DKK1,the above effects of ICA on glycolysis were abolished.CONCLUSION ICA exerts neuroprotective effects on Aβ25-35-induced HT22 cell injury by enhancing the glycolysis function through the activation of the Wnt/β-catenin signaling pathway.