Objective To compare the clinical efficacy of moxifloxacin vs.cefoperazone sodium/tazobactam sodium in the treatment of genitourinary infection,and provide a reference for clinical rational use of antimicrobial drugs through pharmacoeconomics studies.Methods A total of 115 cases with genitourinary infection were randomly divided into moxifloxacin group (59 cases) and cefoperazone sodium/tazobactam sodium group (cephalosporin group,56 cases) by random digits table method.Moxifloxacin group was treated with moxifloxacin hydrochloride tablets,cephalosporin group was given cefoperazone sodium/tazobactam sodium by intravenous infusion for 7 d.The clinical efficacy and incidence of adverse reaction was compared,and the two drugs were evaluated by pharmacoeconomic study.Results The clinical total effective rate in moxifloxacin group and cephalosporin group was respectively 86.4％ (51/59) and 85.7％(48/56),and bacteriological eradication rate was respectively 89.3％(67/75) and 88.9％(64/72),and the difference between the two groups was not statistically significant (P ＞ 0.05).The incremental cost-effectiveness ratios in moxifloxacin group and cephalosporin group were 2.29 and 21.56,and the difference was statistically significant (P ＜0.01).The incidence of adverse reactions in moxifloxacin group and cephalosporin group was 6.8％ (4/59),3.6％ (2/56),and there was no significant difference (P ＞ 0.05).Conclusion In the treatment of genitourinary infections,moxifloxacin and cefoperazone sodium/tazobactam sodium has equivalent efficacy,but moxifloxacin is good to reduce the cost of treatment and is easy to take,which is better treatment programs.

AIM: To observe the expression of hypoxia inducible factor-1? (HIF-1?) gene and heme oxygenase-1 (HO-1) gene in pulmonary arteries in hypoxic rats. METHODS: Forty male Wistar rats were exposed to hypoxia for 0, 3, 7, 14 or 21 days. Mean pulmonary pressure (mPAP), vessel morphometry, right ventricle hypertrophy index (RVHI) were measured. Lungs were either inflation fixed for immunohistochemistry and in situ hybridization or frozen for later measurement of HO-1 enzyme activity. RESULTS: During hypoxia, mPAP increased to significantly higher values than the control values after 7-day of hypoxia,reaching its peak after 14-day of hypoxia, then remained on the high level. Pulmonary artery remodeling developed significantly after 14-day of hypoxia. Expression of HIF-1? protein in control was poorly positive, but was up-regulated in pulmonary arterial tunica intimae of all hypoxic rats. In pulmonary arterial tunica media, the levels of HIF-1? protein were markedly up-regulated after 3-day and 7-day of hypoxia, then tended to decline after 14-day and 21-day of hypoxia. HIF-1? mRNA staining was poorly positive in control, hypoxia for 3 days and hypoxia for 7 days, but began to enhance significantly after 14-day of hypoxia, then remained stable. Expression of HO-1 protein began to increase after 7-day of hypoxia, reaching its peak after 14-day of hypoxia, then remained stable. Expression of HO-1 mRNA began to increase after 3-day of hypoxia, reaching its peak after 7-day of hypoxia, then declined. CONCLUSION: HIF-1? and HO-1 are both involved in the pathogenesis of hypoxia-induced pulmonary hypertension in rats. Furthermore, HIF-1? may inter-regulate with HO-1 gene in this process.

Polybrominated diphenyl ethers (PBDEs) is a kind of important industrial flame retardants,which widely exists in the environment and biosomes.Some researches have indicated that PBDEs is toxic to the nervous system of animals through affecting the release of neuroendocrine hormone,the signal transduction pathways,the transmission of neurotransmitters,the expression of key proteins in the nervous system,and the apoptosis of neuronal cells.Data from environmental monitoring indicated that environmental exposure to PBDEs in China exists,but the research on the effect of PBDEs on nervous system is very limited,which calls for attention.This article summarized researches on the mechanism of neurotoxic effects induced by polybrominated diphenyl ethers from the aspects such as the release of neuroendocrinal hormone,the signal transduction pathways,the transmission of neurotransmitters,the expression of key proteins in the nervous system,and the apoptosis of neuronal cells,and some ideas about the direction of related researches were presented.

To predict the trend of chaotic time series in time series analysis and time series data mining fields, a novel predicting algorithm of chaotic time series trend is presented, and an on-line segmenting algorithm is proposed to convert a time series into a binary string according to ascending or descending trend of each subsequence. The on-line segmenting algorithm is independent of the prior knowledge about time series. The naive Bayesian algorithm is then employed to predict the trend of chaotic time series according to the binary string. The experimental results of three chaotic time series demonstrate that the proposed method predicts the ascending or descending trend of chaotic time series with few error.

ObjectiveTo investigate the effects of fluoxetine on viability and lipopolysaccharide (LPS)-induced TNF-α release of primary cultured rat astrocytes.MethodsThe cells were plated on 96-well tissue culture plates and treated with (5,10,20,40) μM fluoxetine for 24 hours,MTT method was used to measure the cell viability.The cells were plated on 48-well tissue culture plates,treated sequentially with (5,10,20,40) μM fluoxetine and 1 μg/ml LPS,and enzymatic linked immunosorbent assay(ELISA) was used to detect the TNF-α level of the cell supematant.ResultsCompared to viability of the control group( OD value:0.20 ± 0.017 ),fluoxetine at the dose of 20 μM,40 μM increased the viability of astrocytes ( OD value:0.23 ± 0.013,0.24 ± 0.012 ) (P ＜0.05,P＜0.01 ).Treatment with LPS for 24 hours,the level of TNF-α was significantly increased ( 53.84 ±24.84) pg/ml compared to the control group( 8.00 ± 10.87)pg/ml (P ＜ 0.01 ),fluoxetine at a dose of 10 μM can suppressed LPS-induced TNF-α release from astrocytes ( 28.85 ± 3.36 ) pg/ml (P ＜ 0.05 ).ConclusionFluoxetine can increase astrocytes viability and suppress LPS-induced TNF-α release from astrocytes.

To evaluate the effects of calcium on the time course and potency of depolarizing and non depolarizing relaxants, 12 ASA grade Ⅰ Ⅱ patients were randomly divided into two groups. After anesthesia, succinycholine was administered intravenous in a dose of 1mg/kg in group S and vecuronium was administered in a dose of 0 05mg/kg in group V, respectively. 5% calcium chloride 20ml dissolved in 100ml saline were intravenously injected at the same time. The concentration of blood calcium and neuromuscular function (TOF) were monitored. The results showed: (1) Both blood pressure and heart rate were increased in patients of two groups at 2 to 5min after calcium administration, and recovered to normal level 10min later. (2) The concentrations of blood total calcium and dissociated calcium were increased at 5min after calcium chloride administration and maintained to 10min. (3) The time of onset of effect of succinylcholine showed no changes, but the clinical action time and the recovery time were shortened after calcium administration. The recovery time of vecuronium was also obviously shortened by calcium chloride. It suggested that calcium can affect the time course and potency of both depolarizing and non depolarizing relaxants.

To predict the trend of chaotic time series in time series analysis and time series data mining fields, a novel predicting algorithm of chaotic time series trend is presented, and an on-line segmenting algorithm is proposed to convert a time series into a binary string according to ascending or descending trend of each subsequence. The on-line segmenting algorithm is independent of the prior knowledge about time series. The naive Bayesian algorithm is then employed to predict the trend of chaotic time series according to the binary string. The experimental results of three chaotic time series demonstrate that the proposed method predicts the ascending or descending trend of chaotic time series with few error.

BACKGROUND:Different methods of internal fixation can be selected during the repair of femoral shaft fractures in the clinic, including bridging combined internal fixation and metal locking plate screw fixation.
OBJECTIVE: To compare the biomechanical properties of bridging combined internal fixation and metal locking plate screw fixation for femoral shaft fracture.
METHODS:Eighteen femoral samples were colected and randomly divided to three groups, with six in each group. In the normal control group, no treatment was given. In the other groups, models of femoral shaft fracture were established. In the bridging combination group, samples underwent bridging combined internal fixation. In the locking plate screw group, samples underwent metal locking plate screw fixation. Biomechanical test was conducted in samples of the three groups. In the femoral compression experiments, we recorded the maximum displacement under the maximal load 500 N. In the femoral flexion experiment, we recorded the maximum displacement under the maximal load of 100 N. In the femoral retroversion experiment, we recorded the maximum displacement under the maximal load of 100 N.
RESULTS AND CONCLUSION: (1) Compression experiment at different loads: the maximum displacement was larger in the bridging combination group and locking plate screw group than in the normal control group. Under the maximum load of 500 N, the maximum displacement was significantly larger in the bridging combination group and locking plate screw group than in the normal control group (P < 0.05). Moreover, the maximum displacement was significantly less in the bridging combination group than in the locking plate screw group (P< 0.05). (2) Flexion experiment under different loads: the maximum displacement was larger in the bridging combination group and locking plate screw group than in the normal control group. Under the maximal load of 100 N, the maximum displacement was significantly larger in the bridging combination group and locking plate screw group than in the normal control group (P< 0.05). Moreover, the maximum displacement was significantly smaler in the bridging combination group than in the locking plate screw group (P < 0.05). (3) Retroflexion experiment under different loads: the maximum displacement was larger in the bridging combination group and locking plate screw group than in the normal control group. Under the maximal load of 100 N, the maximum displacement was significantly larger in the bridging combination group and locking plate screw group than in the normal control group (P < 0.05). Moreover, the maximum displacement was significantly smaler in the bridging combination group than in the locking plate screw group (P < 0.05). (4) These results suggested thatin vitro simulated femoral shaft fracture bridging combination fixation and metal locking plate screw system can obtain good fixation effect; the design meets the principle of biomechanics. Among them, bridging combined internal fixation can maintain a smaler displacement, suggesting good deformation resistance under compressive stress, anteflexion and retroflexion stress.

Objective To investigate the biological effect of transforming growth factor-?1(TGF-?1) on inducing myofibroblast formation in hypoxic pulmonary vascular remodeling.Methods Forty male Wistar rats were exposed to hypoxia for 0,3,7,14 or 21 days.Mean pulmonary arterial pressure(mPAP),vessel morphometry,right ventricle hypertrophy index(RVHI) were measured.Immunocytochemistry was used to measure the expression of ?-Smooth-muscle actin(?-SMA) and TGF-?1 in pulmonary artery walls and in situ hybridization was used to measure the expression of TGF-?1 mRNA in pulmonary artery walls.Ultrastructure alveolar wall vessels were observed by electron microscopy.Human embryonic lung fibroblasts(KMB17) phenotype after induction of hypoxiaand TGF-?1 were recorded through cell culture.Results(1) mPAP increased significantly after 7-day of hypoxia(P

1.0 ?g/ml) and there was no difference between the young and aged. Conclusions Etomidate and ketmaine have inhibitive effects on proliferation of lymphocytes and there is no difference between the young and aged.