1.The preparation of chitosan-polyvinyl alcohol membrane
Yinghong DING ; Aifen HUANG ; Wanxiang LI ;
Chinese Journal of Marine Drugs 2000;0(06):-
A New Combination membrane was prepared by chitosan and polyvinyl alcohol (PVA).The gas permeability and water content of the membrane are all improved better than that of simple chitosan membrane.
2.Expression of HSP70,c-fos following focal cerebral ischemic reperfusion in rats and neuroprotective effect of Cudrania tricuspidata
Lin SUN ; Aifen LIU ; Yizhao LI
Journal of Clinical Neurology 2001;0(05):-
Objective To investigate the expression of heat shock protein 70(HSP70) and c-fos,the presence of apoptosis and the neuroprotective effects of Cudrania tricuspidata root extrate(Ecr) after focal cerebral ischemic reperfusion in rats.Methods Models of middle cerebral artery occlusion(MCAO) were used in this study.The rats in Ecr pretreated ischemia reperfusion(IR) group were fed with Ecr(2 ml tid) for 5 days before MCAO.The brain specimen were took at different reperfusion times: 1 h,6 h,12 h,24 h,3 d and 7 d after recirculation.Then immunohistochemistry,insitu hybridization,terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL) were used to detect the expression of HSP70,c-fos gene and cell apoptosis in the brains.Positive results were semiquantitatively analyzed.Results IR could induce the expression of HSP70 and c-fos.HSP70 was detected at 6 h following reperfusion and peaked at 24 h both in cortical and basal ganglia regions.c-fos was markedly expressed at 1 h and the level peaked at 6 h in the ischemic hemispheres,and then reduced gradually.The TUNEL positive cells were markedly observed at 6 h.After treatment with Ecr,the positive reaction cells both of HSP70 and c-fos were significantly increased(all(P
3.STUDIES ON FLASK FERMENTATION OF TRACE ELEMENT CR-RICH YEAST
Aifen LI ; Zuli SUN ; Min CHEN ; Huijun ZHIA ; Jiangtao LU ;
Microbiology 1992;0(03):-
From 6 stains of yeasts, Saccharomyces cerevisiae 2016 which was suitable for Cr rich yeasts fermentation was selected Concentration and additive method of CrCl 3 were investigated by liquid culture and the optimum conditions of Cr rich yeasts were obtained The optimum concentration of CrCl 3 were 5?10 4 mol/L that were added to liquid medium at the initial 8 h of culture time At this condition, the biomass of Cr rich yeasts was 1 55 g dry weight per 100 milliliter, Cr content in yeast cells was above 1100 ?g/g, the utilizable rate of Cr ion was about 60%
4.Observation on curative effect of invigorating spleen qi, bushen fillixi combined with oxaliplatin+CF/5-FU on colorectal cancer
Yufen XU ; Binbin SONG ; Xia LI ; Aifen WANG ; Xiaofang XU ; Qiang ZHOU ; Yiming JIANG ; Xinmei YANG
Chinese Journal of Biochemical Pharmaceutics 2017;37(6):74-75
Objective To investigate the therapeutic effect of Jianpi Yiqi, Bushen Fenjing combined with oxaliplatin+CF/5-FU on colorectal cancer.MethodsEighty patients with colorectal cancer who were treated in our hospital from January 2014 to December 2015 were randomly divided into observation group(40cases) and control group (40cases).The control group was given oxaliplatin+CF/5-FU treatment, the observation group in the control group based on the application of Spleen Qi, Bushen fill essence treatment, observed before and after treatment of two groups of serum tumor markers, clinical symptoms and living conditions, changes, record adverse reactions.ResultsThe observation effect of 82.50% was significantly higher than that of the control group (62.50%).The QOL score (37.8±5.6) was significantly higher than that of the control group (33.5±3.1) and the adverse reaction rate was 30.00% (P<0.05).The difference between the two groups was significant (P<0.05).ConclusionThe effect of invigorating spleen qi and tonifying siren solution combined with oxaliplatin+CF/5-FU on colorectal cancer can improve the clinical symptoms and improve the quality of life of patients.
5.Investigation and Analysis of Internships and Employment Intention of Pharmacy Junior Students in Our University
Zhuo DU ; Feidie LIU ; Jianfeng WU ; Haiyan LI ; Xin NIE ; Zhifang CEN ; Weiguang LIANG ; Aifen YAN ; Bin HAN ; Zhong LI
China Pharmacy 2017;28(12):1588-1591
OBJECTIVE:To provide evidence for strengthening education and training before clinical practice and employment guidance among pharmacy junior students of our university. METHODS:Anonymous questionnaire survey was conducted for our university. Questionnaire survey included student employment intention and internship units,relationship of internships with employ-ment,the tendency of employment after graduation,personal attitude about pharmaceutical professional development prospect,ex-pected monthly income in the first year of their career,etc. RESULTS:Totally 71 questionnaires were sent out,71 were effectively received. Among all respondents,74.3%(52 students)believed employment intention had great relationship with internship units;24.3%(17 students)thought employment intention had not great relationship with internship units;moreover,one student thought employment intention had no any relationship with internship units. Most students(94.3%,66 students)with good attitude on in-ternships believed it could accumulate work experience;32.9%(23 students) thought they could stay in the internship unit after practice;moreover,17.1%(12 students) believed practice had little effect on employment. Among them,94.0%(63 students) tended to be employed after graduation,and only 6.0% chose a graduate school to continue their studies. Among the students who chose employment after graduation,76.1%(51 students)tended to engage in pharmaceutical related work,while 17.9%(12 stu-dents) tended to be engaged in work nothing to do with pharmacy or self-employed. The majority (69.6%,48 students) believed that pharmaceutical major prospect was general and pharmaceutical major development varied from person to person;17.4%(12 students)thought pharmaceutical major had good prospect and was promising;13.0%(9 students)believed that pharmaceutical ma-jor had no good prospect and didn't know its prospect. Most of the students(78.2%,54 students)expected a monthly income of 2500-5000 yuan in the first year of their career;18.9%expected a monthly income more than 5000 yuan(13 students);the minor-ity expected a monthly income of 1500-2500 yuan or more than 8000 yuan. CONCLUSIONS:The view of students on internship and employment have a certain gap with the social situation. Related departments of colleges and universities need to adjust the thought of students and strengthen guidance.
6.Effects of nitrogen, manganese, and sulfur deprivation on photo-hydrogen evolution and growth of Chlorella pyrenoidosa.
Lei ZHANG ; Min SANG ; Aifen LI ; Chengwu ZHANG
Chinese Journal of Biotechnology 2010;26(4):489-494
We studied the hydrogen evolution (HE) of green alga Chlorella pyrenoidosa grown in normal (nutrients sufficient) media and nitrogen, manganese or sulfur deprived medium. The results showed that photo-hydrogen evolution could occur under all conditions herein before, but the efficiency of HE was maximum under nitrogen deprivation, and the total hydrogen yield was 88.613 microL H2/mg Chla, which was 4.61, 1.92, 3.63 times of control, manganese deprivation, sulfur deprivation groups, respectively. We also measured the growth, the photosynthesis and respiration of the alga. The data demonstrated that manganese deprivation had less influence than nitrogen and sulfur deprivation on the growth, the photosynthesis and respiration of C. pyrenoidosa. Compared with the normal (nutrients sufficient), manganese and sulfur deprivation inhibited the photosynthesis and growth of the alga while bringing small impact on respiration. Nitrogen deprivation, however, greatly restrained the photosynthesis and growth while enhancing the respiration. Those data provide clues for the further study on both the conditions optimization and mechanism of hydrogen evolution.
Chlorella
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growth & development
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metabolism
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Hydrogen
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analysis
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metabolism
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Manganese
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analysis
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metabolism
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Nitrogen
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analysis
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metabolism
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Photosynthesis
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Sulfur
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analysis
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metabolism
7.Research progress of protein kinase C in liver diseases
Zhangzhang SONG ; Aifen ZHENG ; Wenyan QIN ; Guojun LI ; Zhi CHEN
Journal of Chinese Physician 2019;21(1):150-155
Protein kinase C (PKC) is a special kinase widely distributed in various tissues and cells of human body and involved in signal transduction of hormones and cytokines.It plays an important role in the pathogenesis of many diseases.Therefore,altering the activity of protein kinase C may be an effective treatment for many diseases.This article reviews the progress of protein kinase C in liver diseases.