Objective:To do research on designing the new applied multifunctional integrated tubular pipeline in femoral artery and vein which combined with blood transfusion, fluid infusion, blood sampling, administration, measurement of central venous pressure. Methods: Pipeline is consisted of three parts, which are blood transfusion part and fluid infusion part, including three way switch and three way extended tube. The first way connected with the injection is for blood sampling and doses, the second way connected with the central venous is for measurement of central venous pressure, and the third way connected with the blood transfusion set and fluid infusion set is for blood transfusion and fluid infusion. In a standard thoraco-abdominal combined injury and revivification of 31 beagle, compare the time effect. Results: In the injury and revivification test, the average time cost in 532 times nursing care operation with the multifunctional integrated tubular pipeline is 19+5.1 seconds, the average time cost in 31 times individual nursing care operation of 5 procedure is 80+12 seconds. The multifunctional integrated tubular pipeline can quickly and effectively work on blood transfusion, fluid infusion, blood sampling, administration, measurement of central venous pressure. Conclusion:The multifunctional integrated tubular pipeline in femoral artery and vein saves time and human resources and reduces the infection rate in repeated venous puncture and works quickly and conveniently on animal experiment and acute fatal injury, so it is worthy a further clinical application.

Objective: To verify that COX-2 promoter can drive its downstream genes specifically in COX-2-positive o-varian cancer cells; Moreover, comparing with CMV promoter, analyze the transcript efficiency of COX-2 promoter. Methods: Contacting the recombinant plasmids named COX-2-BAX and CMV-Luc. After transient transfection liposome-mediated with the plasmids COX-2-Luc and CMV-Luc, respectively, the expression of Luciferase reporter gene was measured in COX-2-positive ovarian cancer cell line-SKOV3 and COX-2-negative colon cancer cell line-SW480. SKOV-3 and SW480 were transfected with COX-2-BAX and CMV-BAX in the same way, respectively. The apoptosis rates were measured through flow cytometry. Results: The recombinant plasmids named COX-2-BAX and CMV-Luc were constructed successfully. The expression efficiency of reporter gene was 1554 ? 86. 5 in SKOV3 and 53. 7 ? 10.9 in SW480 after 24 hours transfected with phPES2, 9851. 7 ? 129. 5 in SKOV3 and 8831. 0 ? 167. 3 in SW480 after 24 hours transfected with CMV-Luc in the same way. The apoptosis rate was 10.4% in SKOV3 and 3.7% in SW480 after transfected with COX-2-BAX, 21.7%in SKOV3 and 25. 6% in SW480 after 36 hours transfected with pcDNA3-BAX in the same way. Conclusions: COX-2 promoter can drive its downstream genes specifically in COX-2-positive ovarian cancer cell lines, but its expression efficiency wasmarkedly lower than CMV promoters. With proper modification, COX-2 promoter is expected to be useful in gene therapy of ovarian cancers.

With the gradual increase of China's total expenditure on research and development (R&D),the national megaprojects implementation of state-dominated megaprojects should solve major national problems and meet the national great strategic demand according joint research of multiunit and multi-discipline.The importance of management for research cooperation agreement and business contracts are increasingly prominent.Based on the author's own experience,a scientific research institute's perspective,contractual agreements on the management audit and review the content of the key points are summarized.It gives the reference for the staff of scientific management.

Objective To research the electrocardiogram (ECG) manifestations of common electrolyte disturbance and to discuss the accuracy of ECG diagnosis. Methods One hundred and sixty-nine hospitalized patients with hyperkalemia and 170 patients with hypokalemia were selected and the results of ECG were analyzed. Results In mild hyperkalemia patients (serum potassium concentration 5.5- 6.5 mmol/L), the ECG abnormal rate was 91.4% (64/70). In mild hypokalemia patients (serum potassium concentration 3.0-3.5 mmol/L), the ECG abnormal rate was 56.9% (41/72). There was significant difference (P < 0.05). The greater the deviation of serum potassium concentration, the more obvious the characteristic changes of ECG. Conclusions In addition to the blood biochemical test, ECG is also a kind of check tool that can help to diagnose abnormal blood potassium.

Objective To analyze the clinical effect of community comprehensive intervention in the prevention and treatment of hypertension.Methods A total of 160 cases of patients were randomly divided into two groups,the control group (80 cases)were treated with conventional antihypertensive drug treatment;the observation group 80 cases of patients were treated with comprehensive community intervention on the basis of treatment of the control group.Two groups of patients before and after the intervention of blood pressure changes were observed and recorded,treatment effect and medication compliance were comparative analysized.Results The total effective rate of patients in the observation group was 86.25%,which was significantly higher than that in the control group (57.5%),the difference was statistically significant (χ2 =20.44,P <0.05).DBP,SBP after intervention of patients in the observation group were significantly lower than those before intervention and in the control group after the intervention,the differences were statistically significant (t =18.48,6.06,all P <0.05).Conclusion The effect of community comprehensive intervention on hypertension patients is significant,it can effectively control the patient's blood pressure level,improve the quality of life of patients,which is worth promoting.

Objective To investigate the geographic distribution of 5-HT2A T102C genotypes frequency in the PLA schizophrenia patients. Methods The 5-HT2A receptor gene T102C polymorphism was analyzed for genotyping by the method of polymerase chain reaction(PCR)in 177 male PLA schizophrenia patients. Results The distribution of 5-HT2A genotypic frequencies in 177 male PLA schizophrenia patients were 0.07~0.33(? 2=4.44,P=0.617 1) for A1A1, 0.50~0.72 for A1A2 (? 2=1.14, P=0.942 2), and 0.17~0.29 for A2A2 (? 2=0.93, P=0.985 7), respectively. There was no significant correlation between 5-HT2A frequencies and different populations. Conclusion The frequencies of 5-HT2A T102C genotypes in the PLA schizophrenia patients were uniform in different populations.

The aim of the study was to evaluate the efficacy and safety of phloroglucinol in treatment of abdominal pain caused by acute gastroenteritis.Three hundred patients with acute abdominal pain were randomly assigned to receive 80 mg intravenous infusion of phloroglucinol injection ( treatment group,n =150 ) or 10 mg anisodamine injection (control group,n =150).The pain intensity was estimated by the VAS scores before and after the injection,meanwhile adverse reactions were also recorded.The results showed that the pain intensity was relieved significantly after 1h of the injection in both groups(P ＜0.01).The effective rate of phloroglucinol was 84.1％ (126/150),while that of anisodamine was 80.0％ (120/150).The adverse reactions of treatment group were lower than that of control group( all P ＜0.01).The results indicate that phloroglucinol can effectively relieve abdominal pain caused by acute gastroenteritis with safety.

Objeetlve To evaluate the relationship between temporomandibular joint(TMJ)osteoarthrosis (OA)and cytokines.Methods 20 rabbits were enrolled in this study.They were divided into either experiment(n=10)or control group(n=10).The modeh of TMJ osteoarthrosis were formed by injection SMT into upper joint compartment of the TMJ.Cytokines in the aspirated synovial fluid,induding interleukin(IL-1),IL-6,tumor necrosis faetor(TNF-α),were tested before and after SMT iniection.Results The level of IL-1β,IL-6 and TNF-α in experiment group decreased significantly after treatment,there was significance(P＜0.05);and the level of IL-1β,IL-6 and TNF-α in control group is lower after treatment,there was no significance(P＞0.05).Conclusion The cytokines,including IL-1β,IL-6,TNF-α,decreased significantly by injection SMT into upper joint compartment of the TMJ with OA.

Objective To investigate the variation patterm in activity concentration of 210Pb in atmospheric aerosol in Qindao and evaluate its internal radiation effect to human body.Methods 210Pb activity concentration of aerosol samples was measured by high purity germanium(HPGe) γ spectrometer for purpose of ascertaining its variation in aerosol in Qingdao from April 2015 to March 2016.Results 210Pb activity concentration in aerosol varied from 0.06 to 1.61 mBq/m3 and the mean of (0.70 ± 0.50) mBq/m3,slightly higher than the UNSECAR 1988 report recommended value of O.50 mBq/m3.In this study,210Pb activity concentration in aerosol was high from December 2015 to February 2016 and low from August to November.Radiation dose assessment revealed that the annual committed effective dose to adults delivered by 210Pb was 6.35 × 10-6 Sv.The monthly variation in activity concentration variation of 210Pb in atmospheric aerosol in Qingdao was similar as in Xiamen.Conclusions Activity concentration of 210Pb in atmospheric aerosols in Qingdao varied largely dependent on atmospheric environmental pollution.210Pb contributed less to the adult annual committed effective dose.However,more attention should be drawn to the long-term radiation effects to aduhs due to the 210Pb potentially deposited in lungs.

Febrile infection -related epilepsy syndrome(FIRES)is a newly named entity of epileptic encepha-lopathy in recent years.As one of the hot spots studied by domestic and foreign scholars,the etiology and pathogenesis of FIRES,which has a poor treatment and outcome,are undefined.It manifests with refractory seizures and status epilep-ticus in previously healthy school -aged children after banal febrile illness.In addition,with the negative response to currently available treatments,there are a poor prognosis and a high mortality rate in FIRES.Survivors are typically left with refractory partial epilepsy and severe cognitive impairment.Through domestic and foreign literature,this article re-views the potential pathogenesis,clinical features,treatment and prognosis of the syndrome,and provides theoretical ba-sis and new ideas for clinical diagnosis and treatment.