1.Studies on the Quality Control Standard of Adansan Eye-drops
China Pharmacy 1991;0(05):-
OBJECTIVE:To establish the quality standard of Adansan eye-drops(ADS).METHODS:Saliva miltiorrhiza and ATP were qualitatively detected by TLC and HPLC respectively.Atropine sulfate was quantitatively detected by VIS-spectrophotometry.RESULTS:The average recovery was 99.9% with a RSD of 2.46%(n=5).CONCLUSION:This method is simple,accurate and specific.It can be used for the quality control of ADS.
2.Determination of Content of Ceftizoxime by RP-HPLC
Aichun YI ; Shunzhi ZHANG ; Difang CHEN
China Pharmacy 2001;0(11):-
OBJECTIVE:To determine the content of ceftizoxime for injection METHODS:A RP-HPLC method was established,the polarls C18 was used as analysis column The mobile phase consisted of 0 23% sodium hydrogen phosphate and 0 14% trisodium buffer-acetonitrile(85∶15),the flow rate was 1 0ml/min,the detection wavelength was 254nm RESULTS:The linear range of ceftizoxime was 4 1 462~37 3 156?g/ml,r=0 9 999 The relative standard deviation was 0 6% CONCL_USION:The method is rapid,accurate and suitable for determination of content of ceftizoxime for injection
3.Effect of integrated nursing intervention on postoperative recovery of supracondylar humerus fractures with ulnar deviation in children
Jianfeng MA ; Tina ZHANG ; Aichun XING ; Jing DOU
Chinese Journal of Practical Nursing 2017;33(22):1690-1693
Objective To observe the effect of comprehensive nursing of traditional Chinese medicine and Western Medicine on children′s supracondylar humerus fracture of ulnar deviation on postoperative recovery. Methods From November 2015 to February 2016, 52 inpatients were divided into 2 groups by random digits table method after the external fixation removal surgery with 26 cases each. The observation group was intervened by integrated nursing and was given TCM rehabilitation guidance, while the control group was intervened by regular nursing and was given regular rehabilitation guidance. After 4 weeks, the therapeutic effect was evaluated according to Chinese Medicine Orthopedic Syndrome Diagnosis and their elbow joint function was evaluated by Flynn score. In 8 weeks of follow up, all the patients were valued by their quality of life. Results In the process of intervention, with 1 case fell off in the observation group, 3 cases fell off in the control group, so finally the observation group with 25 cases, the control group with 23 cases. Fifteen cases were cured, 7 cases were improved, 3 cases were not cured at 4 weeks after surgery in the observation group, 8, 6, 9 cases in the control group, and the difference was statistically significant (Z=-2.120, P=0.034). At 4 weeks after surgery, 10, 6, 6, 3 cases were excellent, good, medium and poor evaluated by Flynn score in the observation group, 5, 4, 5, 9 cases in the control group, and the difference was statistically significant (Z=-2.051, P=0.040). Incidence of postoperative complications was 8.0% (2/25) in the observation group, 30.4% (7/23) in the control group, and the difference was statistically significant (χ2=2.622, P=0.047). Physical, psychological, social and environmental fields of quality of life were 77.84 ± 12.15, 81.13 ± 15.72, 79.95 ± 20.68, 79.44 ± 11.57 in the observation group, 63.31 ± 15.86, 63.07 ± 12.63, 65.65 ± 18.51, 61.32 ± 17.24 in the control group, and the difference was statistically significant (t=2.516- 4.363, P<0.05). Conclusions Integrated nursing intervention with TCM rehabilitation guidance is a method which can effectively help in improving the elbow movement function and the life quality of children with supracondylar humerus fractures of ulnar deviation.
4.Multiple myeloma presented as polyart hritis: case reports and literature review
Yanying LIU ; Li ZHANG ; Aichun LIU ; Hua YE ; Yuhui LI ; Zhanguo LI
Chinese Journal of Rheumatology 2014;18(3):195-198
Objective We reported 5 unusual cases of multiple myeloma (MM) initially presented as polyarthritis.Special attention should be paid to this very unusual scenario.Methods Five cases who presented initially as polyarthritis and finally diagnosed as MM were administered to Peking University People's Hospital and 11 cases from literature were reported.The clinical symptoms and laboratory parameters were analyzed respectively.Results The primarily affected joints were small joints,such as metacarpophalangeal joints,proximal interphalangeal joint,metatarsophalangeal and temporomandibular joints,but large joints such as wrists,elbows,shoulders,knees,ankle joints could be involved,too.Fourteen of 16 patients were misdiagnosed as rheumatoid arthritis (RA).Renal dysfunction presenting as high level of serum creatinine or proteinuria was detected in 14 cases.Laboratory tests showed elevated erythrocyte sedimentation rate or C reactive protein level in 13 cases.Anemia was detected in 9 cases.Hypercalcemia was detected in half of the patients.Hyperglobu-linemia was detected in 6 cases.The serum level of free M-protein was markedly elevated in 14 cases.Auto-antibodies were negative,except that rheumatoid factor was positive in 1 case.Conclusion It is important to pay attention to the clinical pictures presented as polyarthritis with negative autoantibodies,and associated with renal dysfunction,anemia and hypergammaglobulinemia.MM is important in the differential diagnosis.
6. The clinical application of color Doppler ultrasound in guiding the puncture of new internal fistula in deep arteriovenous
Aichun WU ; Huang ZHANG ; Lei WU
Chinese Journal of Practical Nursing 2020;36(1):36-41
Objective:
To investigate the clinical value of color Doppler ultrasound guidance in the puncture of new internal fistula in deep arteriovenous of hemodialysis patients.
Methods:
Selected thirty patients with arteriovenous fistula not less than 5 mm deep in the Department of Hemodialysis Room of Wuhan First Hospital, and they were divided into observation group and control group by the method of random number generator in IBM SPSS Statistics 22.0 software, with 15 patients in each group. The control group was treated with conventional blind puncture by experienced nurses (849 times), while the observation group was treated with color Doppler ultrasound guided puncture (855 times). By comparing two groups of deep arteriovenous fistula in new fistula patients within 6 months of the one-time success rate of puncture, the incidence of adverse events such as subcutaneous hematoma, bleeding, pain, interruption or postponement of treatment, and accidental injury to arteries caused by puncture errors of internal fistula, and the patient′s satisfaction with the puncture and dialysis results was evaluated to observe the advantage of color Doppler ultrasound guidance in clinical application of deep arteriovenous new fistula puncture.
Results:
The success rate of one-time puncture for internal fistula puncture in observation group was 98.95% (846/855), which was significantly higher than that in control group 81.27% (690/849), and the difference was statistically significant (
7.Application of p16 and Ki67 protein in diagnosis of cervical lesions
Pingping ZHONG ; Yiqun GU ; Aichun WANG ; Xiaofei SUN ; Yingnan WANG ; Junling XIE ; Hui ZHANG
Chinese Journal of Primary Medicine and Pharmacy 2015;22(4):524-527
Objective To investigate the diagnosis value of p16 combined with Ki67 protein in cervical lesions.Methods Totally 1 542 women with previous liquid-based cytology smear result of abnormality underwent a colposcopy-directed biopsy excision procedure.Biopsy specimens were detected by p16 and Ki67 immunostaining alongside hematoxylin and eosin (H&E) staining.A four-semiquantitative class was used to describe the immunohistochemical results.Results Biopsy results revealed 1 542 women included 473 women with negative for dysplasia (NEG),629 women with cervical intraepithelial neoplasia (CIN) Ⅰ,206 women with CIN Ⅱ,206 women with CINⅢ and 28 women with cervical squamous cell carcinoma (SCC).The averageage of this study population was 34.47 years.CINs mainly occurred in women aged 20-29 years and 30-39 years.The positive rates of p16 in NEG,CIN Ⅰ,CIN Ⅱ,CINⅢ and SCC were 15.22%,60.25%,98.06%,99.51%,100.00% respectively,and the positive rates of Ki67 were 12.05%,63.12%,96.12%,98.06%,100.00% respectively.p16 expression and Ki-67 expression significantly increased with disease progression (p16:r =0.758,P =0.000 ; Ki67:r =0.773,P =0.000).Expression level of p16 was positively related with Ki-67 (r =0.774,P =0.000).The positive expression rates of p16 and Ki-67 of NEG were significantly lower than those of CIN and SCC (p16:x2 =1 127.46,P =0.000;Ki67:x2 =1 316.85,P =0.000).The positive expression rates of p16 and Ki-67 were markedly higher in CIN Ⅰ than those in CINⅡ,CINⅢⅢ and SCC (p16:x2 =500.19,P =0.000;Ki67:x2 =603.23,P=0.000).Conclusion Women aged 20-39 years are key subjects for cervical cancer screening.p16 and Ki67 immunohistochemistry is important in the ancillary diagnosis of cervical lesions.
8.Analysis of prognostic patients with non-ST-segment elevation acute myocardial infarction and clinical characteristics of those with renal dysfunction
Huinan ZHU ; Yong HUO ; Jie JIANG ; Yan ZHANG ; Aichun KANG ; Hua HE
Journal of Peking University(Health Sciences) 2003;0(05):-
Objective:To evaluate the clinical features of non-ST-segment-elevation myocardial infarction(NSTEMI)patients with renal dysfunction and investigate correlation factor for in-hospital death and 6 months adverse events of NSTEMI patients.Methods:One hundred and sixteen patients presenting with NSTEMI were enrolled between January 2006 and September 2007.Estimation of glomerular filtrate rate(eGFR)was conducted by the modified abbreviated MDRD equations based on the Chinese CKD patients.Renal dysfunction was defined as eGFR
9.Research progress on chemical composition, analytical methods, and pharmacological effects of Ophiopogon polysaccharides
Luxin ZHANG ; Xueqian ZHOU ; Dekun LI ; Dazheng ZHOU ; Yuewu YANG ; Boyang YU ; Aichun JU
Drug Evaluation Research 2017;40(2):279-284
The main ingredients of Ophiopogonjaponicas include steroidal saponins,flavonoids,polysaccharides,and so on,but the research on polysaccharides is relatively less.This particle will mainly review the chemical composition,analytical methods,and pharmacological effects of Ophiopogon polysaccharides.The Ophiopogon polysaccharides contain a variety of substances,such as MDG-1,Md-1,Md-2,OJP-1,etc;The main analysis methods were sulfuric acid method,phenol-sulfuric acid method,3,5-dinitrosalicylic acid (DNS) colorimetric method,and near-infrared spectroscopy combined with partial least squares regression method.Ophiopogon polysaccharides can effectively improve the cardiovascular system diseases,with antihypoxia,anti-inflammatory,antitumor,anti-oxidation,and other pharmacological effects.
10.Chemical constituents of Alisma orientalis and their immunosuppressive function.
Chaofeng ZHANG ; Aichun ZHOU ; Mian ZHANG
China Journal of Chinese Materia Medica 2009;34(8):994-998
OBJECTIVETo investigate the chemical constituents with immunosuppressive function from Alisma orientalis.
METHODThe chemical constituents were isolated and purified by kinds of column chromatography and its structures were elucidated by NMR spectra and physicochemical properties. Its immunocompentence of lymphocytes taken from spleen of mouse were examined by MTT assay.
RESULTTwelve compounds were isolated and identified as clovandiol (1), orientalol E (2), alismoxide (3), alismol (4), 4alpha, l0alpha-dihydroxy-5beta-H-guaj-6-en (5), alismorientols A (6), alisol F (7), alisol A (8), 13beta,17beta-epoxy alisol A (9), alisol B 23-acetate (10), 1H-indole-3-carboxylic acid (11) and cuccinic acid (12). Compounds 9, 10 and alisol A 24-acetate showed immunosuppressive function.
CONCLUSIONCompounds 1, 5, 11 and 12 were isolated firstly from this genus,and the NMR spectra data of 1 were corrected firstly, some protostan-type triterpenoids may be developed as new drug with immunosuppressive function.
Alisma ; chemistry ; Animals ; Cells, Cultured ; Cholestenones ; chemistry ; pharmacology ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; Immunosuppressive Agents ; chemistry ; pharmacology ; Indoles ; chemistry ; pharmacology ; Magnetic Resonance Spectroscopy ; Mice ; Mice, Inbred C57BL ; Sesquiterpenes ; chemistry ; pharmacology ; T-Lymphocytes ; cytology ; drug effects ; Triterpenes ; chemistry ; pharmacology